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Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA.

Abstract：

:Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. As termination is defined by the release of product RNA from the transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as recycling. During the recycling stage, post-terminational RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and can initiate transcription again at the original and nearby promoters in the case of retaining a sigma factor. The efficiency of this event, termed here as reinitiation, increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on the DNA template for reinitiation most of the time.

journal_name

Nat Commun

journal_title

Nature communications

authors

Kang W,Ha KS,Uhm H,Park K,Lee JY,Hohng S,Kang C

doi

10.1038/s41467-019-14200-3

subject

Has Abstract

pub_date

2020-01-23 00:00:00

pages

450

issue

1

issn

2041-1723

pii

10.1038/s41467-019-14200-3

journal_volume

11

pub_type

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