Abstract:
:The reversible modification of cysteine residues by thioester formation with palmitate (S-palmitoylation) is an abundant lipid post-translational modification (PTM) in mammalian systems. S-palmitoylation has been observed on mitochondrial proteins, providing an intriguing potential connection between metabolic lipids and mitochondrial regulation. However, it is unknown whether and/or how mitochondrial S-palmitoylation is regulated. Here we report the development of mitoDPPs, targeted fluorescent probes that measure the activity levels of "erasers" of S-palmitoylation, acyl-protein thioesterases (APTs), within mitochondria of live cells. Using mitoDPPs, we discover active S-depalmitoylation in mitochondria, in part mediated by APT1, an S-depalmitoylase previously thought to reside in the cytosol and on the Golgi apparatus. We also find that perturbation of long-chain acyl-CoA cytoplasm and mitochondrial regulatory proteins, respectively, results in selective responses from cytosolic and mitochondrial S-depalmitoylases. Altogether, this work reveals that mitochondrial S-palmitoylation is actively regulated by "eraser" enzymes that respond to alterations in mitochondrial lipid homeostasis.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Kathayat RS,Cao Y,Elvira PD,Sandoz PA,Zaballa ME,Springer MZ,Drake LE,Macleod KF,van der Goot FG,Dickinson BCdoi
10.1038/s41467-017-02655-1subject
Has Abstractpub_date
2018-01-23 00:00:00pages
334issue
1issn
2041-1723pii
10.1038/s41467-017-02655-1journal_volume
9pub_type
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