Abstract:
:Rosemary extracts containing the phenolic diterpenes carnosic acid and its derivative carnosol are approved food additives used in an increasingly wide range of products to enhance shelf-life, thanks to their high anti-oxidant activity. We describe here the elucidation of the complete biosynthetic pathway of carnosic acid and its reconstitution in yeast cells. Cytochrome P450 oxygenases (CYP76AH22-24) from Rosmarinus officinalis and Salvia fruticosa already characterized as ferruginol synthases are also able to produce 11-hydroxyferruginol. Modelling-based mutagenesis of three amino acids in the related ferruginol synthase (CYP76AH1) from S. miltiorrhiza is sufficient to convert it to a 11-hydroxyferruginol synthase (HFS). The three sequential C20 oxidations for the conversion of 11-hydroxyferruginol to carnosic acid are catalysed by the related CYP76AK6-8. The availability of the genes for the biosynthesis of carnosic acid opens opportunities for the metabolic engineering of phenolic diterpenes, a class of compounds with potent anti-oxidant, anti-inflammatory and anti-tumour activities.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Scheler U,Brandt W,Porzel A,Rothe K,Manzano D,Božić D,Papaefthimiou D,Balcke GU,Henning A,Lohse S,Marillonnet S,Kanellis AK,Ferrer A,Tissier Adoi
10.1038/ncomms12942subject
Has Abstractpub_date
2016-10-05 00:00:00pages
12942issn
2041-1723pii
ncomms12942journal_volume
7pub_type
杂志文章abstract::Interaction between light and matter results in new quantum states whose energetics can modify chemical kinetics. In the regime of ensemble vibrational strong coupling (VSC), a macroscopic number [Formula: see text] of molecular transitions couple to each resonant cavity mode, yielding two hybrid light-matter (polarit...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12636-1
更新日期:2019-10-15 00:00:00
abstract::In most metazoan nuclei, heterochromatin is located at the nuclear periphery in contact with the nuclear lamina, which provides mechanical stability to the nucleus. We show that in cultured cells, chromatin decompaction by the nucleosome binding protein HMGN5 decreases the sturdiness, elasticity and rigidity of the nu...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7138
更新日期:2015-01-22 00:00:00
abstract::The use of hyperpolarized agents in magnetic resonance, such as (13)C-labelled compounds, enables powerful new imaging and detection modalities that stem from a 10,000-fold boost in signal. A major challenge for the future of the hyperpolarization technique is the inherently short spin-relaxation times, typically <60 ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9459
更新日期:2015-10-09 00:00:00
abstract::Phonons are the main source of relaxation in molecular nanomagnets, and different mechanisms have been proposed in order to explain the wealth of experimental findings. However, very limited experimental investigations on phonons in these systems have been performed so far, yielding no information about their dispersi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15475-7
更新日期:2020-04-09 00:00:00
abstract::For the large-scale sustainable implementation of polymer electrolyte membrane fuel cells in vehicles, high-performance electrocatalysts with low platinum consumption are desirable for use as cathode material during the oxygen reduction reaction in fuel cells. Here we report a carbon black-supported cost-effective, ef...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15938
更新日期:2017-07-24 00:00:00
abstract::A 'pseudogap' was introduced by Mott to describe a state of matter that has a minimum in the density of states at the Fermi level, deep enough for states to become localized. It can arise either from Coulomb repulsion between electrons, and/or incipient charge or spin order. Here we employ ultrafast spectroscopy to st...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7958
更新日期:2015-04-20 00:00:00
abstract::Synthetic genetic circuits allow us to modify the behavior of living cells. However, changes in environmental conditions and unforeseen interactions with the host cell can cause deviations from a desired function, resulting in the need for time-consuming reassembly to fix these issues. Here, we use a regulatory motif ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15653-7
更新日期:2020-04-29 00:00:00
abstract::Phospholipase C is a key enzyme in phosphoinositide turnover. Although its functions have been extensively studied at the cellular level, many questions remain concerning its functions at the organ and individual animal levels. Here we demonstrate that mice lacking phospholipase Cδ1 develop granulocytosis associated w...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1960
更新日期:2012-07-17 00:00:00
abstract::Theoretical and experimental results have revealed that the lithium-ion storage capacity for nitrogen-doped graphene largely depends on the nitrogen-doping level. However, most nitrogen-doped carbon materials used for lithium-ion batteries are reported to have a nitrogen content of approximately 10 wt% because a highe...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6261
更新日期:2014-11-06 00:00:00
abstract::Mammalian and Drosophila genomes are partitioned into topologically associating domains (TADs). Although this partitioning has been reported to be functionally relevant, it is unclear whether TADs represent true physical units located at the same genomic positions in each cell nucleus or emerge as an average of numero...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20292-z
更新日期:2021-01-04 00:00:00
abstract::A major conundrum in climate science is how to account for dependence between climate models. This complicates interpretation of probabilistic projections derived from such models. Here we show that this problem can be addressed using a novel method to test multiple non-exclusive hypotheses, and to make predictions un...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10561-x
更新日期:2019-07-09 00:00:00
abstract::Blood vessel expansion is driven by sprouting angiogenesis of endothelial cells, and is essential for development, wound healing and disease. Membrane-localized vascular endothelial growth factor receptor-1 (mVEGFR1) is an endothelial cell-intrinsic decoy receptor that negatively modulates blood vessel morphogenesis. ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15699
更新日期:2017-06-07 00:00:00
abstract::Electrodynamic responses from three-dimensional topological insulators are characterized by the universal magnetoelectric term constituent of the Lagrangian formalism. The quantized magnetoelectric coupling, which is generally referred to as topological magnetoelectric effect, has been predicted to induce exotic pheno...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12245
更新日期:2016-07-20 00:00:00
abstract::Epithelial barrier function is maintained by coordination of cell proliferation and cell loss, whereas barrier dysfunction can lead to disease and organismal death. JNK signalling is a conserved stress signalling pathway activated by bacterial infection and tissue damage, often leading to apoptotic cell death and comp...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14289
更新日期:2017-03-08 00:00:00
abstract::Familial hemiplegic migraine is an episodic neurological disorder characterized by transient sensory and motor symptoms and signs. Mutations of the ion pump α2-Na/K ATPase cause familial hemiplegic migraine, but the mechanisms by which α2-Na/K ATPase mutations lead to the migraine phenotype remain incompletely underst...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19915-2
更新日期:2020-12-02 00:00:00
abstract::The degradation of intrinsic properties of graphene during the transfer process constitutes a major challenge in graphene device fabrication, stimulating the need for direct growth of graphene on dielectric substrates. Previous attempts of metal-induced transformation of diamond and silicon carbide into graphene suffe...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12099
更新日期:2016-07-04 00:00:00
abstract::Global climate change has intensified the need to assess the capacity for natural populations to adapt to abrupt shifts in the environment. Reductions in seawater pH constitute a conspicuous global change stressor that is affecting marine ecosystems globally. Here, we quantify the phenotypic and genetic modifications ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13767-1
更新日期:2019-12-20 00:00:00
abstract::Striated muscle contraction is regulated by the translocation of troponin-tropomyosin strands over the thin filament surface. Relaxation relies partly on highly-favorable, conformation-dependent electrostatic contacts between actin and tropomyosin, which position tropomyosin such that it impedes actomyosin association...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15922-5
更新日期:2020-05-15 00:00:00
abstract::The Dansgaard-Oeschger (DO) events, as observed in oxygen isotope ratios from the North Greenland Ice Core Project (NGRIP) record, are an outstanding example of past abrupt climate transitions. Their physical cause remains debated, and previous research indicated that they are not preceded by classical early-warning s...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04881-7
更新日期:2018-07-02 00:00:00
abstract::The power conversion efficiency of hybrid solid-state solar cells has more than doubled from 7 to 15% over the past year. This is largely as a result of the incorporation of organometallic trihalide perovskite absorbers into these devices. But, as promising as this development is, long-term operational stability is ju...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3885
更新日期:2013-01-01 00:00:00
abstract::In bacteria, transcription complexes stalled on DNA represent a major source of roadblocks for the DNA replication machinery that must be removed in order to prevent damaging collisions. Gram-positive bacteria contain a transcription factor HelD that is able to remove and recycle stalled complexes, but it was not know...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20157-5
更新日期:2020-12-18 00:00:00
abstract::With damage tolerance rivalling advanced engineering alloys and thermoplastic forming capabilities analogous to conventional plastics, metallic glasses are emerging as a modern engineering material. Here, we take advantage of their unique electrical and rheological properties along with the classic Lorentz force conce...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10576
更新日期:2016-02-08 00:00:00
abstract::In mammals, a subset of arginine tRNA isoacceptors are methylated in the anticodon loop by the METTL2 methyltransferase to form the 3-methylcytosine (m3C) modification. However, the mechanism by which METTL2 identifies specific tRNA arginine species for m3C formation as well as the biological role of m3C in mammals is...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16321-6
更新日期:2020-05-19 00:00:00
abstract::The RNA-binding protein RC3H1 (also known as ROQUIN) promotes TNFα mRNA decay via a 3'UTR constitutive decay element (CDE). Here we applied PAR-CLIP to human RC3H1 to identify ∼ 3,800 mRNA targets with >16,000 binding sites. A large number of sites are distinct from the consensus CDE and revealed a structure-sequence ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8367
更新日期:2015-07-14 00:00:00
abstract::This corrects the article DOI: 10.1038/ncomms15317. ...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/ncomms16128
更新日期:2017-06-30 00:00:00
abstract::The secretome of cancer and stromal cells generates a microenvironment that contributes to tumour cell invasion and angiogenesis. Here we compare the secretome of human mammary normal and cancer-associated fibroblasts (CAFs). We discover that the chloride intracellular channel protein 3 (CLIC3) is an abundant componen...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14206
更新日期:2017-02-15 00:00:00
abstract::Transition metal reagents and catalysts are generally effective to cleave all three bonds (one σ and two π) in a triple bond despite its high bonding energy. Recently, chemistry of single-bond cleavage by using main-group element compounds is rapidly being developed in the absence of transition metals. However, the cl...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5245
更新日期:2014-06-26 00:00:00
abstract::Calcium ions (Ca(2+)) function as universal second messengers in eukaryotic cells, including immune cells. Ca(2+) is crucial for peripheral T-lymphocyte activation and effector functions, and influences thymocyte selection and motility in the developing thymus. However, the role of Ca(2+) signalling in early T-lymphoc...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5814
更新日期:2014-09-12 00:00:00
abstract::Rhinovirus (RV) infections are major triggers of acute exacerbations of severe respiratory diseases such as pre-school wheeze, asthma and chronic obstructive pulmonary disease (COPD). The occurrence of numerous RV types is a major challenge for the identification of the culprit virus types and for the improvement of v...
journal_title:Nature communications
pub_type: 临床试验,杂志文章
doi:10.1038/s41467-018-04591-0
更新日期:2018-06-18 00:00:00
abstract::Nanocrystal quantum dots are attractive materials for applications as nanoscale light sources. One impediment to these applications is fluctuations of single-dot emission intensity, known as blinking. Recent progress in colloidal synthesis has produced nonblinking nanocrystals; however, the physics underlying blinking...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1916
更新日期:2012-06-19 00:00:00