Abstract：
:Cell-free DNA in the blood provides a non-invasive diagnostic avenue for patients with cancer1. However, characteristics of the origins and molecular features of cell-free DNA are poorly understood. Here we developed an approach to evaluate fragmentation patterns of cell-free DNA across the genome, and found that profiles of healthy individuals reflected nucleosomal patterns of white blood cells, whereas patients with cancer had altered fragmentation profiles. We used this method to analyse the fragmentation profiles of 236 patients with breast, colorectal, lung, ovarian, pancreatic, gastric or bile duct cancer and 245 healthy individuals. A machine learning model that incorporated genome-wide fragmentation features had sensitivities of detection ranging from 57% to more than 99% among the seven cancer types at 98% specificity, with an overall area under the curve value of 0.94. Fragmentation profiles could be used to identify the tissue of origin of the cancers to a limited number of sites in 75% of cases. Combining our approach with mutation-based cell-free DNA analyses detected 91% of patients with cancer. The results of these analyses highlight important properties of cell-free DNA and provide a proof-of-principle approach for the screening, early detection and monitoring of human cancer.
journal_name
Naturejournal_title
Natureauthors
Cristiano S,Leal A,Phallen J,Fiksel J,Adleff V,Bruhm DC,Jensen SØ,Medina JE,Hruban C,White JR,Palsgrove DN,Niknafs N,Anagnostou V,Forde P,Naidoo J,Marrone K,Brahmer J,Woodward BD,Husain H,van Rooijen KL,Ørntoft MWdoi
10.1038/s41586-019-1272-6subject
Has Abstractpub_date
2019-06-01 00:00:00pages
385-389issue
7761eissn
0028-0836issn
1476-4687pii
10.1038/s41586-019-1272-6journal_volume
570pub_type
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