Abstract：
:The cellular responses induced by mitochondrial dysfunction remain elusive. Intrigued by the lack of almost any glomerular phenotype in patients with profound renal ischemia, we comprehensively investigated the primary sources of energy of glomerular podocytes. Combining functional measurements of oxygen consumption rates, glomerular metabolite analysis, and determination of mitochondrial density of podocytes in vivo, we demonstrate that anaerobic glycolysis and fermentation of glucose to lactate represent the key energy source of podocytes. Under physiological conditions, we could detect neither a developmental nor late-onset pathological phenotype in podocytes with impaired mitochondrial biogenesis machinery, defective mitochondrial fusion-fission apparatus, or reduced mtDNA stability and transcription caused by podocyte-specific deletion of Pgc-1α, Drp1, or Tfam, respectively. Anaerobic glycolysis represents the predominant metabolic pathway of podocytes. These findings offer a strategy to therapeutically interfere with the enhanced podocyte metabolism in various progressive kidney diseases, such as diabetic nephropathy or focal segmental glomerulosclerosis (FSGS).
journal_name
Cell Repjournal_title
Cell reportsauthors
Brinkkoetter PT,Bork T,Salou S,Liang W,Mizi A,Özel C,Koehler S,Hagmann HH,Ising C,Kuczkowski A,Schnyder S,Abed A,Schermer B,Benzing T,Kretz O,Puelles VG,Lagies S,Schlimpert M,Kammerer B,Handschin C,Schell C,Hubedoi
10.1016/j.celrep.2019.04.012subject
Has Abstractpub_date
2019-04-30 00:00:00pages
1551-1566.e5issue
5issn
2211-1247pii
S2211-1247(19)30467-Xjournal_volume
27pub_type
杂志文章相关文献
Cell Reports文献大全abstract：:Oncogenic mutations of FLT3 and KIT receptors are associated with poor survival in patients with acute myeloid leukemia (AML) and myeloproliferative neoplasms (MPNs), and currently available drugs are largely ineffective. Although Stat5 has been implicated in regulating several myeloid and lymphoid malignancies, how p...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.10.039
更新日期：2014-11-20 00:00:00
abstract：:The diversity and complexity of the cancer transcriptome may contain transcripts unique to the tumor environment. Here, we report a LIN28B variant, LIN28B-TST, which is specifically expressed in hepatocellular carcinoma (HCC) and many other cancer types. Expression of LIN28B-TST is associated with significantly poor p...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.02.002
更新日期：2018-02-20 00:00:00
abstract：:Lung disease is a major cause of death in the United States, with current therapeutic approaches serving only to manage symptoms. The most common chronic and life-threatening genetic disease of the lung is cystic fibrosis (CF) caused by mutations in the cystic fibrosis transmembrane regulator (CFTR). We have generated...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.07.062
更新日期：2015-09-01 00:00:00
abstract：:Although DNA methylation is commonly invoked as a mechanism for transcriptional repression, the extent to which it actively silences transcription factor (TF) occupancy sites in vivo is unknown. To study the role of DNA methylation in the active modulation of TF binding, we quantified the effect of DNA methylation dep...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.07.024
更新日期：2015-08-18 00:00:00
abstract：:Neural modulation in primate motor cortex exhibits complex patterns. We found that modulation during reaching could be separated into discrete temporal epochs. To determine if these epochs are driven by behavioral events, monkeys performed variations of a center-out reaching task. Monkeys viewed a computer cursor matc...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.11.069
更新日期：2019-12-17 00:00:00
abstract：:Tumor suppressor p53 regulates transcription of stress-response genes. Many p53 targets remain undiscovered because of uncertainty as to where p53 binds in the genome and the fact that few genes reside near p53-bound recognition elements (REs). Using chromatin immunoprecipitation followed by exonuclease treatment (ChI...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.06.030
更新日期：2014-07-24 00:00:00
abstract：:Selecting a suitable site to deposit their eggs is an important reproductive need of Drosophila females. Although their choosiness toward egg-laying sites is well documented, the specific neural mechanism that activates females' search for attractive egg-laying sites is not known. Here, we show that distention and con...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.09.033
更新日期：2014-10-23 00:00:00
abstract：:The flat bones of the skull are densely innervated during development, but little is known regarding their role during repair. We describe a neurotrophic mechanism that directs sensory nerve transit in the mouse calvaria. Patent cranial suture mesenchyme represents an NGF (nerve growth factor)-rich domain, in which se...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107696
更新日期：2020-05-26 00:00:00
abstract：:The role of CTCF in stabilizing long-range interactions between chromatin sites essential for maintaining nuclear architecture is well established. Most of these interactions involve recruitment of the cohesin complex to chromatin via CTCF. We find that CTCF also interacts with the centromeric protein CENP-E both in v...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.08.005
更新日期：2015-09-08 00:00:00
abstract：:The regulation of innervation by target-derived factors like nerve growth factor (NGF) is the cornerstone of neurotrophic theory. Whereas autocrine signaling in neurons affecting survival and axon growth has been described, it is difficult to reconcile autocrine signaling with the idea that targets control their inner...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.02.016
更新日期：2015-03-10 00:00:00
abstract：:The relationship between the host and its microbiota is challenging to understand because both microbial communities and their environments are highly variable. We have developed a set of techniques based on population dynamics and information theory to address this challenge. These methods identify additional bacteri...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.12.088
更新日期：2016-02-02 00:00:00
abstract：:Hypertrophic cardiomyopathy (HCM) is a heritable cardiovascular disorder that affects 1 in 500 people. A significant percentage of HCM is attributed to mutations in β-cardiac myosin, the motor protein that powers ventricular contraction. This study reports how two early-onset HCM mutations, D239N and H251N, affect the...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.11.040
更新日期：2016-12-13 00:00:00
abstract：:The RNA binding proteins Rbfox1/2/3 regulate alternative splicing in the nervous system, and disruption of Rbfox1 has been implicated in autism. However, comprehensive identification of functional Rbfox targets has been challenging. Here, we perform HITS-CLIP for all three Rbfox family members in order to globally map...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.02.005
更新日期：2014-03-27 00:00:00
abstract：:Protein-coding genes undergo a wide array of regulatory interactions with factors that engage non-coding regions. Open reading frames (ORFs), in contrast, are thought to be constrained by coding function, precluding a major role in gene regulation. Here, we explore Piwi-interacting (pi)RNA-mediated transgene silencing...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.02.009
更新日期：2018-02-27 00:00:00
abstract：:Nonalcoholic fatty liver disease is the most common chronic liver disorder in developed countries. Its pathogenesis is poorly understood, and therapeutic options are limited. Here, we show that SIRT7, an NAD(+)-dependent H3K18Ac deacetylase, functions at chromatin to suppress ER stress and prevent the development of f...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.10.007
更新日期：2013-11-14 00:00:00
abstract：:The neural network of the temporal lobe is thought to provide a cognitive map of our surroundings. Functional analysis of this network has been hampered by coarse tools that often result in collateral damage to other circuits. We developed a chemogenetic system to temporally control electrical input into the hippocamp...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.06.032
更新日期：2016-07-19 00:00:00
abstract：:Electrons feed into the mitochondrial electron transport chain (mETC) from NAD- or FAD-dependent enzymes. A shift from glucose to fatty acids increases electron flux through FAD, which can saturate the oxidation capacity of the dedicated coenzyme Q (CoQ) pool and result in the generation of reactive oxygen species. To...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.03.009
更新日期：2016-04-05 00:00:00
abstract：:Metazoan genomes are partitioned into modular chromosomal domains containing active or repressive chromatin. In flies, Polycomb group (PcG) response elements (PREs) recruit PHO and other DNA-binding factors and act as nucleation sites for the formation of Polycomb repressive domains. The sequence specificity of PREs i...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.08.072
更新日期：2014-10-09 00:00:00
abstract：:The conserved NAD+-dependent deacylase SIRT1 plays pivotal, sometimes contrasting, roles in diverse physiological and pathophysiological conditions. In this study, we uncover a tissue-restricted isoform of SIRT1 (SIRT1-ΔE2) that lacks exon 2 (E2). Candidate-based screening of SIRT1 substrates demonstrated that the dom...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.03.012
更新日期：2017-03-28 00:00:00
abstract：:The deadly malaria parasite Plasmodium falciparum contains a nonphotosynthetic plastid, known as the apicoplast, that functions to produce essential metabolites, and drugs that target the apicoplast are clinically effective. Several prokaryotic caseinolytic protease (Clp) genes have been identified in the Plasmodium g...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.10.081
更新日期：2017-11-14 00:00:00
abstract：:The local synthesis of transmembrane proteins underlies functional specialization of dendritic microdomains in neuronal plasticity. It is unclear whether these proteins have access to the complete machinery of the secretory pathway following local synthesis. In this study, we describe a probe called pGolt that allows ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.12.024
更新日期：2016-01-12 00:00:00
abstract：:The central mechanism by which neurotensin (Nts) potentiates weight loss has remained elusive. We leveraged chemogenetics to reveal that Nts-expressing neurons of the lateral hypothalamic area (LHA) promote weight loss in mice by increasing volitional activity and restraining food intake. Intriguingly, these dual weig...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.11.068
更新日期：2017-12-12 00:00:00
abstract：:The thalamus plays a central role in sleep rhythms in the mammalian brain and, yet, surprisingly little is known about its function and interaction with local cortical oscillations during NREM sleep (NREM). We investigated the neuronal correlates of cortical barrel activity in the two corresponding thalamic nuclei, th...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.01.038
更新日期：2019-02-05 00:00:00
abstract：:Calcium is a key regulator of mitochondrial function under both normal and pathological conditions. The mechanisms linking metabolic activity to mitochondrial Ca2+ signaling remain elusive, however. Here, by monitoring mitochondrial Ca2+ transients while manipulating mitochondrial membrane potential (ΔΨm), we found th...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.11.084
更新日期：2018-12-18 00:00:00
abstract：:Mouse embryonic stem cells are dynamic and heterogeneous. For example, rare cells cycle through a state characterized by decondensed chromatin and expression of transcripts, including the Zscan4 cluster and MERVL endogenous retrovirus, which are usually restricted to preimplantation embryos. Here, we further character...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.08.087
更新日期：2016-09-27 00:00:00
abstract：:Most tumor cells exhibit obligatory demands for essential amino acids (EAAs), but the regulatory mechanisms whereby tumor cells take up EAAs and EAAs promote malignant transformation remain to be determined. Here, we show that oncogenic MYC, solute carrier family (SLC) 7 member 5 (SLC7A5), and SLC43A1 constitute a fee...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.12.002
更新日期：2017-12-26 00:00:00
abstract：:The Fanconi anemia (FA) pathway repairs DNA interstrand crosslinks (ICLs). Many FA proteins are recruited to ICLs in a timely fashion so that coordinated repair can occur. However, the mechanism of this process is poorly understood. Here, we report the purification of a FANCD2-containing protein complex with multiple ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107850
更新日期：2020-07-07 00:00:00
abstract：:Although vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are under development, the antigen epitopes on the virus and their immunogenicity are poorly understood. Here, we simulate the 3D structures and predict the B cell epitopes on the spike (S), envelope (E), membrane (M), and nucleocap...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108666
更新日期：2021-01-26 00:00:00
abstract：:High-throughput imaging has led to an explosion of observations about cell-size homeostasis across the kingdoms of life. Among bacteria, "adder" behavior-in which a constant size increment appears to be added during each cell cycle-is ubiquitous, while various eukaryotes show other size-homeostasis behaviors. Since in...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107992
更新日期：2020-08-11 00:00:00
abstract：:Replication of a damaged DNA template can threaten the integrity of the genome, requiring the use of various mechanisms to tolerate DNA lesions. The Smc5/6 complex, together with the Nse2/Mms21 SUMO ligase, plays essential roles in genome stability through undefined tasks at damaged replication forks. Various subunits...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.10.123
更新日期：2019-12-03 00:00:00
