Abstract：
:Protein-coding genes undergo a wide array of regulatory interactions with factors that engage non-coding regions. Open reading frames (ORFs), in contrast, are thought to be constrained by coding function, precluding a major role in gene regulation. Here, we explore Piwi-interacting (pi)RNA-mediated transgene silencing in C. elegans and show that marked differences in the sensitivity to piRNA silencing map to the endogenous sequences within transgene ORFs. Artificially increasing piRNA targeting within the ORF of a resistant transgene can lead to a partial yet stable reduction in expression, revealing that piRNAs not only silence but can also "tune" gene expression. Our findings support a model that involves a temporal element to mRNA regulation by germline Argonautes, likely prior to translation, and suggest that piRNAs afford incremental control of germline mRNA expression by targeting the body of the mRNA, including the coding region.
journal_name
Cell Repjournal_title
Cell reportsauthors
Seth M,Shirayama M,Tang W,Shen EZ,Tu S,Lee HC,Weng Z,Mello CCdoi
10.1016/j.celrep.2018.02.009subject
Has Abstractpub_date
2018-02-27 00:00:00pages
2254-2264issue
9issn
2211-1247pii
S2211-1247(18)30177-3journal_volume
22pub_type
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