Abstract：
:The local synthesis of transmembrane proteins underlies functional specialization of dendritic microdomains in neuronal plasticity. It is unclear whether these proteins have access to the complete machinery of the secretory pathway following local synthesis. In this study, we describe a probe called pGolt that allows visualization of Golgi-related organelles for live imaging in neurons. We show that pGolt labels a widespread microsecretory Golgi satellite (GS) system that is, in contrast to Golgi outposts, present throughout basal and apical dendrites of all pyramidal neurons. The GS system contains glycosylation machinery and is localized between ERGIC and retromer. Synaptic activity restrains lateral movement of ERGIC, GS, and retromer close to one another, allowing confined processing of secretory cargo. Several synaptic transmembrane proteins pass through and recycle back to the GS system. Thus, the presence of an ER-ERGIC-GS-retromer microsecretory system in all neuronal dendrites enables autonomous local control of transmembrane protein synthesis and processing.
journal_name
Cell Repjournal_title
Cell reportsauthors
Mikhaylova M,Bera S,Kobler O,Frischknecht R,Kreutz MRdoi
10.1016/j.celrep.2015.12.024subject
Has Abstractpub_date
2016-01-12 00:00:00pages
189-99issue
2issn
2211-1247pii
S2211-1247(15)01437-0journal_volume
14pub_type
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