Ecdysone-Induced 3D Chromatin Reorganization Involves Active Enhancers Bound by Pipsqueak and Polycomb.

abstract：:Transgenic zebrafish embryos expressing tissue-specific green fluorescent protein (GFP) can provide an unlimited supply of primary embryonic cells. Agents that promote the differentiation of these cells may be beneficial for therapeutics. We report a high-throughput approach for screening small molecules that regulate...

journal_title：Cell reports

authors： Huang H,Lindgren A,Wu X,Liu NA,Lin S

更新日期：2012-09-27 00:00:00

abstract：:High-grade serous ovarian carcinoma (HGSOC) originates mainly from the fallopian tube (FT) epithelium and always carries early TP53 mutations. We previously reported that tumors initiate in the FT fimbria epithelium because of apoptotic failure and the expansion of cells with DNA double-strand breaks (DSB) caused by b...

journal_title：Cell reports

authors： Wu NY,Huang HS,Chao TH,Chou HM,Fang C,Qin CZ,Lin CY,Chu TY,Zhou HH

更新日期：2017-03-14 00:00:00

abstract：:Intracellular transport and homeostasis of the endomembrane system in eukaryotic cells depend on the formation and fusion of vesicular carriers. Coat protein complex (COP) II vesicles export newly synthesized secretory proteins from the endoplasmic reticulum (ER), whereas COPI vesicles facilitate traffic from the Golg...

journal_title：Cell reports

authors： Adolf F,Rhiel M,Hessling B,Gao Q,Hellwig A,Béthune J,Wieland FT

更新日期：2019-01-02 00:00:00

abstract：:Wnts are a family of 19 extracellular ligands that regulate cell fate, proliferation, and migration during metazoan embryogenesis and throughout adulthood. Wnts are acylated post-translationally at a conserved serine and bind the extracellular cysteine-rich domain (CRD) of Frizzled (FZD) seven-pass transmembrane recep...

journal_title：Cell reports

authors： Speer KF,Sommer A,Tajer B,Mullins MC,Klein PS,Lemmon MA

更新日期：2019-01-22 00:00:00

abstract：:In metazoans, the nuclear envelope (NE) breakdown (NEBD) occurs during "open" mitosis and meiosis. In the fission yeast Schizosaccharomyces pombe, the mitosis and the first meiotic division (MI) are "closed," during which the NE is maintained. Intriguingly, during the second meiotic division (MII), the NE is also main...

journal_title：Cell reports

authors： Flor-Parra I,Iglesias-Romero AB,Salas-Pino S,Lucena R,Jimenez J,Daga RR

更新日期：2018-04-24 00:00:00

abstract：:Regulation of DNA replication and cell division is essential for tissue growth and maintenance of genomic integrity and is particularly important in tissues that undergo continuous regeneration such as mammary glands. We have previously shown that disruption of the KRAB-domain zinc finger protein Roma/Zfp157 results i...

journal_title：Cell reports

authors： Ho TLF,Guilbaud G,Blow JJ,Sale JE,Watson CJ

更新日期：2016-04-26 00:00:00

abstract：:GABAergic synapses in brain circuits generate inhibitory output signals with submillisecond latency and temporal precision. Whether the molecular identity of the release sensor contributes to these signaling properties remains unclear. Here, we examined the Ca2+ sensor of exocytosis at GABAergic basket cell (BC) to Pu...

journal_title：Cell reports

authors： Chen C,Arai I,Satterfield R,Young SM Jr,Jonas P

更新日期：2017-01-17 00:00:00

abstract：:Fragile X syndrome (FXS) is the leading heritable cause of intellectual disability and commonly co-occurs with autism spectrum disorder. Silencing of the Fmr1 gene leads to the absence of the protein product, fragile X mental retardation protein (FMRP), which represses translation of many target mRNAs. Excess translat...

journal_title：Cell reports

authors： Russo A,DiAntonio A

更新日期：2019-09-03 00:00:00

abstract：:Prior studies have shown that aversive olfactory memory is acquired by dopamine acting on a specific receptor, dDA1, expressed by mushroom body neurons. Active forgetting is mediated by dopamine acting on another receptor, Damb, expressed by the same neurons. Surprisingly, prior studies have shown that both receptors ...

journal_title：Cell reports

authors： Himmelreich S,Masuho I,Berry JA,MacMullen C,Skamangas NK,Martemyanov KA,Davis RL

更新日期：2017-11-21 00:00:00

abstract：:Thymic epithelial cells (TECs) are critically required for T cell development, but the cellular mechanisms that maintain adult TECs are poorly understood. Here, we show that a previously unidentified subpopulation, EpCam(+)UEA1(-)Ly-51(+)PLET1(+)MHC class II(hi), which comprises <0.5% of adult TECs, contains bipotent ...

journal_title：Cell reports

authors： Ulyanchenko S,O'Neill KE,Medley T,Farley AM,Vaidya HJ,Cook AM,Blair NF,Blackburn CC

更新日期：2016-03-29 00:00:00

abstract：:The two related members of the vasohibin family, VASH1 and VASH2, encode human tubulin detyrosinases. Here we demonstrate that, in contrast to VASH1, which requires binding of small vasohibin binding protein (SVBP), VASH2 has autonomous tubulin detyrosinating activity. Moreover, we demonstrate that SVBP acts as a bona...

journal_title：Cell reports

authors： van der Laan S,Lévêque MF,Marcellin G,Vezenkov L,Lannay Y,Dubra G,Bompard G,Ovejero S,Urbach S,Burgess A,Amblard M,Sterkers Y,Bastien P,Rogowski K

更新日期：2019-12-17 00:00:00

abstract：:Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a stringent protocol to...

journal_title：Cell reports

authors： Fritzsche R,Karra D,Bennett KL,Ang FY,Heraud-Farlow JE,Tolino M,Doyle M,Bauer KE,Thomas S,Planyavsky M,Arn E,Bakosova A,Jungwirth K,Hörmann A,Palfi Z,Sandholzer J,Schwarz M,Macchi P,Colinge J,Superti-Furga G,Kiebl

更新日期：2013-12-26 00:00:00

abstract：:Molecular processes involving lncRNAs regulate cell function. By applying transcriptomics, we identify lncRNAs whose expression is regulated by transforming growth factor β (TGF-β). Upon silencing individual lncRNAs, we identify several that regulate TGF-β signaling. Among these lncRNAs, TGFB2-antisense RNA1 (TGFB2-AS...

journal_title：Cell reports

authors： Papoutsoglou P,Tsubakihara Y,Caja L,Morén A,Pallis P,Ameur A,Heldin CH,Moustakas A

更新日期：2019-09-17 00:00:00

abstract：:Virus infection is sensed by pattern recognition receptors (PRRs) detecting virus nucleic acids and initiating an innate immune response. DNA-dependent protein kinase (DNA-PK) is a PRR that binds cytosolic DNA and is antagonized by vaccinia virus (VACV) protein C16. Here, VACV protein C4 is also shown to antagonize DN...

journal_title：Cell reports

authors： Scutts SR,Ember SW,Ren H,Ye C,Lovejoy CA,Mazzon M,Veyer DL,Sumner RP,Smith GL

更新日期：2018-11-13 00:00:00

abstract：:Nuclear actin has been elusive due to the lack of knowledge about molecular mechanisms. From actin-containing chromatin remodeling complexes, we discovered an arginine mono-methylation mark on an evolutionarily conserved R256 residue of actin (R256me1). Actin R256 mutations in yeast affect nuclear functions and cause ...

journal_title：Cell reports

authors： Kumar A,Zhong Y,Albrecht A,Sang PB,Maples A,Liu Z,Vinayachandran V,Reja R,Lee CF,Kumar A,Chen J,Xiao J,Park B,Shen J,Liu B,Person MD,Trybus KM,Zhang KYJ,Pugh BF,Kamm KE,Milewicz DM,Shen X,Kapoor P

更新日期：2020-09-29 00:00:00

abstract：:Understanding how p53 activates certain gene programs and not others is critical. Here, we identify low-density lipoprotein receptor-related protein 1 (LRP1), a transmembrane endocytic receptor, as a p53 target gene. We show that, although LRP1 transcript expression is upregulated in response to both sub-lethal and le...

journal_title：Cell reports

authors： Leslie PL,Franklin DA,Liu Y,Zhang Y

更新日期：2018-08-07 00:00:00

abstract：:Ubiquitin is post-translationally modified by phosphorylation at several sites, but the consequences of these modifications are largely unknown. Here, we synthesize multi-milligram quantities of ubiquitin phosphorylated at serine 20, serine 57, and serine 65 via genetic code expansion. We use these phosphoubiquitins f...

journal_title：Cell reports

authors： Huguenin-Dezot N,De Cesare V,Peltier J,Knebel A,Kristaryianto YA,Rogerson DT,Kulathu Y,Trost M,Chin JW

更新日期：2016-07-26 00:00:00

abstract：:The recent success of derivation of mammalian haploid embryonic stem cells (haESCs) has provided a powerful tool for large-scale functional analysis of the mammalian genome. However, haESCs rapidly become diploidized after differentiation, posing challenges for genetic analysis. Here, we show that the spontaneous dipl...

journal_title：Cell reports

authors： He ZQ,Xia BL,Wang YK,Li J,Feng GH,Zhang LL,Li YH,Wan HF,Li TD,Xu K,Yuan XW,Li YF,Zhang XX,Zhang Y,Wang L,Li W,Zhou Q

更新日期：2017-08-29 00:00:00

abstract：:Human rhinovirus (HRV) causes upper respiratory infections and asthma exacerbations. We screened multiple orthologous RNAi reagents and identified host proteins that modulate HRV replication. Here, we show that RNASEK, a transmembrane protein, was needed for the replication of HRV, influenza A virus, and dengue virus....

journal_title：Cell reports

authors： Perreira JM,Aker AM,Savidis G,Chin CR,McDougall WM,Portmann JM,Meraner P,Smith MC,Rahman M,Baker RE,Gauthier A,Franti M,Brass AL

更新日期：2015-08-04 00:00:00

abstract：:In response to DNA double-strand breaks (DSBs), H2AX is rapidly phosphorylated at Ser139 to promote DSB repair. Here we show that H2AX is rapidly stabilized in response to DSBs to efficiently generate γH2AX foci. This mechanism operated even in quiescent cells that barely expressed H2AX. H2AX stabilization resulted fr...

journal_title：Cell reports

authors： Atsumi Y,Minakawa Y,Ono M,Dobashi S,Shinohe K,Shinohara A,Takeda S,Takagi M,Takamatsu N,Nakagama H,Teraoka H,Yoshioka K

更新日期：2015-12-29 00:00:00

abstract：:Calcium signaling has long been associated with key events of immunity, including chemotaxis, phagocytosis, and activation. However, imaging and manipulation of calcium flux in motile immune cells in live animals remain challenging. Using light-sheet microscopy for in vivo calcium imaging in zebrafish, we observe char...

journal_title：Cell reports

authors： Beerman RW,Matty MA,Au GG,Looger LL,Choudhury KR,Keller PJ,Tobin DM

更新日期：2015-12-15 00:00:00

abstract：:Silencing of FMR1 and loss of its gene product, FMRP, results in fragile X syndrome (FXS). FMRP binds brain mRNAs and inhibits polypeptide elongation. Using ribosome profiling of the hippocampus, we find that ribosome footprint levels in Fmr1-deficient tissue mostly reflect changes in RNA abundance. Profiling over a t...

journal_title：Cell reports

authors： Shah S,Molinaro G,Liu B,Wang R,Huber KM,Richter JD

更新日期：2020-03-31 00:00:00

abstract：:Large-scale, cooperative rearrangements underlie the functions of RNA in RNA-protein machines and gene regulation. To understand how such rearrangements are orchestrated, we used high-throughput chemical footprinting to dissect a seemingly concerted rearrangement in P5abc RNA, a paradigm of RNA folding studies. With m...

journal_title：Cell reports

authors： Gracia B,Al-Hashimi HM,Bisaria N,Das R,Herschlag D,Russell R

更新日期：2018-03-20 00:00:00

abstract：:Ectopic expression of transcription factors (TFs) can reprogram cell state. However, because of the large combinatorial space of possible TF cocktails, it remains difficult to identify TFs that reprogram specific cell types. Here, we develop Reprogram-Seq to experimentally screen thousands of TF cocktails for reprogra...

journal_title：Cell reports

authors： Duan J,Li B,Bhakta M,Xie S,Zhou P,Munshi NV,Hon GC

更新日期：2019-06-18 00:00:00

abstract：:Adipocytes deficient in fatty acid synthase (iAdFASNKO) emit signals that mimic cold exposure to enhance the appearance of thermogenic beige adipocytes in mouse inguinal white adipose tissues (iWATs). Both cold exposure and iAdFASNKO upregulate the sympathetic nerve fiber (SNF) modulator Neuregulin 4 (Nrg4), activate ...

journal_title：Cell reports

authors： Henriques F,Bedard AH,Guilherme A,Kelly M,Chi J,Zhang P,Lifshitz LM,Bellvé K,Rowland LA,Yenilmez B,Kumar S,Wang Y,Luban J,Weinstein LS,Lin JD,Cohen P,Czech MP

更新日期：2020-08-04 00:00:00

abstract：:Chromodomain Helicase DNA binding protein 5 (CHD5) is a tumor suppressor mapping to 1p36, a genomic region that is frequently deleted in human cancer. Although CHD5 belongs to the CHD family of chromatin-remodeling proteins, whether its tumor-suppressive role involves an interaction with chromatin is unknown. Here we ...

journal_title：Cell reports

authors： Paul S,Kuo A,Schalch T,Vogel H,Joshua-Tor L,McCombie WR,Gozani O,Hammell M,Mills AA

更新日期：2013-01-31 00:00:00

abstract：:Gambogic acid (GA) is a natural compound derived from Chinese herbs that has been approved by the Chinese Food and Drug Administration for clinical trials in cancer patients; however, its molecular targets have not been thoroughly studied. Here, we report that GA inhibits tumor proteasome activity, with potency compar...

journal_title：Cell reports

authors： Li X,Liu S,Huang H,Liu N,Zhao C,Liao S,Yang C,Liu Y,Zhao C,Li S,Lu X,Liu C,Guan L,Zhao K,Shi X,Song W,Zhou P,Dong X,Guo H,Wen G,Zhang C,Jiang L,Ma N,Li B,Wang S,Tan H,Wang X,Dou QP,Liu J

更新日期：2013-01-31 00:00:00

abstract：:In vivo experimental evidence indicates that acute neuronal activation increases Aβ release from presynaptic terminals, whereas long-term effects of chronic synaptic activation on Aβ pathology remain unclear. To address this issue, we adopted optogenetics and transduced stabilized step-function opsin, a channelrhodops...

journal_title：Cell reports

authors： Yamamoto K,Tanei ZI,Hashimoto T,Wakabayashi T,Okuno H,Naka Y,Yizhar O,Fenno LE,Fukayama M,Bito H,Cirrito JR,Holtzman DM,Deisseroth K,Iwatsubo T

更新日期：2015-05-12 00:00:00

abstract：:Melanomas accumulate a high burden of mutations that could potentially generate neoantigens, yet somehow suppress the immune response to facilitate continued growth. In this study, we identify a subset of human melanomas that have loss-of-function mutations in ATR, a kinase that recognizes and repairs UV-induced DNA d...

journal_title：Cell reports

authors： Chen CF,Ruiz-Vega R,Vasudeva P,Espitia F,Krasieva TB,de Feraudy S,Tromberg BJ,Huang S,Garner CP,Wu J,Hoon DS,Ganesan AK

更新日期：2017-03-07 00:00:00

abstract：:Katanin was the first microtubule (MT)-severing enzyme discovered, but how katanin executes MT severing remains poorly understood. Here, we report X-ray crystal structures of the apo and ATPγS-bound states of the catalytic AAA domain of human katanin p60 at 3.0 and 2.9 Å resolution, respectively. Comparison of the two...

journal_title：Cell reports

authors： Shin SC,Im SK,Jang EH,Jin KS,Hur EM,Kim EE

更新日期：2019-01-29 00:00:00