Abstract:
:The conserved NAD+-dependent deacylase SIRT1 plays pivotal, sometimes contrasting, roles in diverse physiological and pathophysiological conditions. In this study, we uncover a tissue-restricted isoform of SIRT1 (SIRT1-ΔE2) that lacks exon 2 (E2). Candidate-based screening of SIRT1 substrates demonstrated that the domain encoded by this exon plays a key role in specifying SIRT1 protein-protein interactions. The E2 domain of SIRT1 was both necessary and sufficient for PGC1α binding, enhanced interaction with p53, and thus downstream functions. Since SIRT1-FL and SIRT1-ΔE2 were found to have similar intrinsic catalytic activities, we propose that the E2 domain tethers specific substrate proteins. Given the absence of SIRT1-ΔE2 in liver, our findings provide insight into the role of the E2 domain in specifying "metabolic functions" of SIRT1-FL. Identification of SIRT1-ΔE2 and the conserved specificity domain will enhance our understanding of SIRT1 and guide the development of therapeutic interventions.
journal_name
Cell Repjournal_title
Cell reportsauthors
Deota S,Chattopadhyay T,Ramachandran D,Armstrong E,Camacho B,Maniyadath B,Fulzele A,Gonzalez-de-Peredo A,Denu JM,Kolthur-Seetharam Udoi
10.1016/j.celrep.2017.03.012subject
Has Abstractpub_date
2017-03-28 00:00:00pages
3069-3077issue
13issn
2211-1247pii
S2211-1247(17)30329-7journal_volume
18pub_type
杂志文章相关文献
Cell Reports文献大全abstract::Megakaryocytes (MKs) come from a complex process of hematopoietic progenitor maturation within the bone marrow that gives rise to de novo circulating platelets. Bone marrow microenvironment contains a large number of adipocytes with a still ill-defined role. This study aims to analyze the influence of adipocytes and i...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.107875
更新日期:2020-07-07 00:00:00
abstract::Synapse formation is a complex process that involves the recruitment and assembly of a myriad of pre- and postsynaptic proteins. Despite being present at every synapse in the vertebrate CNS, little is known about the transport, recruitment, and stabilization of synapsin at nascent synapses during development. We exami...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.03.031
更新日期:2013-04-25 00:00:00
abstract::Emerging evidence suggests that crosstalk between glioma cells and the brain microenvironment may influence brain tumor growth. To date, known reciprocal interactions among these cells have been limited to the release of paracrine factors. Combining a genetic strategy with longitudinal live imaging, we find that indiv...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.01.089
更新日期:2020-02-25 00:00:00
abstract::Cilia mediate Hedgehog (Hh) signaling in vertebrates and Hh deregulation results in several clinical manifestations, such as obesity, cognitive disabilities, developmental malformations, and various cancers. Drosophila cells are nonciliated during development, which has led to the assumption that cilia-mediated Hh sig...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.03.052
更新日期:2014-05-08 00:00:00
abstract::The plasma membrane (PM) forms extensive close junctions with the cortical endoplasmic reticulum (cER) in many cell types, ranging from yeast to mammals. How cells modulate structural plasticity of ER-PM contacts to accommodate space-demanding cortical events is largely unknown. Here, we report a role for eisosome-dri...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.12.098
更新日期:2020-02-04 00:00:00
abstract::Dynamic nuclear SUMO modifications play essential roles in orchestrating cellular responses to proteotoxic stress, DNA damage, and DNA virus infection. Here, we describe a non-canonical host SUMOylation response to the nuclear-replicating RNA pathogen, influenza virus, and identify viral RNA polymerase activity as a m...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.10.001
更新日期:2015-11-17 00:00:00
abstract::The architecture of renal glomeruli is acquired through intricate and still poorly understood developmental steps. In our study we identify a crucial glomerular morphogenetic event in nephrogenesis that drives the remodeling/separation of the prospective vascular pole (the future entrance of the glomerular arterioles)...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108304
更新日期:2020-10-27 00:00:00
abstract::CRISPR/Cas-based technologies have revolutionized genetic approaches to addressing a wide range of neurobiological questions. The ability of CRISPR/Cas to introduce mutations into target genes allows us to perform in vivo loss-of-function experiments without generating genetically engineered mice. However, the lack of...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.06.038
更新日期:2019-07-09 00:00:00
abstract::Autophagy is an essential cellular survival mechanism that is required for adaptive lymphocyte development; however, its role in innate lymphoid cell (ILC) development remains unknown. Furthermore, the conditions that promote lymphocyte autophagy during homeostasis are poorly understood. Here, we demonstrate that Atg5...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.04.082
更新日期:2016-05-31 00:00:00
abstract::We explored the role of the Krebs cycle enzyme fumarate hydratase (FH) in glucose-stimulated insulin secretion (GSIS). Mice lacking Fh1 in pancreatic β cells (Fh1βKO mice) appear normal for 6-8 weeks but then develop progressive glucose intolerance and diabetes. Glucose tolerance is rescued by expression of mitochondr...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.08.093
更新日期:2017-09-26 00:00:00
abstract::MicroRNAs play an important role in T cell responses. However, how microRNAs regulate CD8 T cell memory remains poorly defined. Here, we found that miR-150 negatively regulates CD8 T cell memory in vivo. Genetic deletion of miR-150 disrupted the balance between memory precursor and terminal effector CD8 T cells follow...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.08.060
更新日期:2017-09-12 00:00:00
abstract::Proper control of DNA replication is essential to ensure faithful transmission of genetic material and prevent chromosomal aberrations that can drive cancer progression and developmental disorders. DNA replication is regulated primarily at the level of initiation and is under strict cell-cycle regulation. Importantly,...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.10.005
更新日期:2014-11-06 00:00:00
abstract::Evidence suggests that Polycomb (Pc) is present at chromatin loop anchors in Drosophila. Pc is recruited to DNA through interactions with the GAGA binding factors GAF and Pipsqueak (Psq). Using HiChIP in Drosophila cells, we find that the psq gene, which has diverse roles in development and tumorigenesis, encodes dist...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.07.096
更新日期:2019-09-03 00:00:00
abstract::The pharyngeal arch arteries (PAAs) are transient embryonic blood vessels that mature into critical segments of the aortic arch and its branches. Although defects in PAA development cause life-threating congenital cardiovascular defects, the molecular mechanisms that orchestrate PAA morphogenesis remain unclear. Throu...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.07.002
更新日期:2017-07-25 00:00:00
abstract::Leptin has beneficial effects on glucose metabolism via actions in the hypothalamus, but the roles of specific subgroups of neurons responsible for these antidiabetic effects remain unresolved. We generated diabetic Lep(ob/ob) or Lepr(db/db) mice lacking or re-expressing leptin receptors (LepRb) in subgroups of neuron...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.04.010
更新日期:2014-05-22 00:00:00
abstract::Polymorphisms that alter serotonin transporter SERT expression and functionality increase the risks for autism and psychiatric traits. Here, we investigate how SERT controls serotonin signaling in developing CNS in mice. SERT is transiently expressed in specific sets of glutamatergic neurons and uptakes extrasynaptic ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.12.033
更新日期:2015-01-20 00:00:00
abstract::Parsing diverse nerve cells into biological types is necessary for understanding neural circuit organization. Morphology is an intuitive criterion for neuronal classification and a proxy of connectivity, but morphological diversity and variability often preclude resolving the granularity of neuron types. Combining gen...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.02.040
更新日期:2019-03-12 00:00:00
abstract::APOBEC family cytidine deaminases have recently been implicated as powerful mutators of cancer genomes. How APOBECs, which are ssDNA-specific enzymes, gain access to chromosomal DNA is unclear. To ascertain the chromosomal ssDNA substrates of the APOBECs, we expressed APOBEC3A and APOBEC3B, the two most probable APOBE...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.01.021
更新日期:2016-02-16 00:00:00
abstract::In humans, Vγ9Vδ2 T cells respond to self and pathogen-associated, diphosphate-containing isoprenoids, also known as phosphoantigens (pAgs). However, activation and homeostasis of Vγ9Vδ2 T cells remain incompletely understood. Here, we show that pAgs induced expression of the ecto-ATPase CD39, which, however, not only...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.06.009
更新日期:2016-07-12 00:00:00
abstract::DNA methylation provides a mechanism by which environmental factors can control insulin sensitivity in obesity. Here, we assessed DNA methylation in skeletal muscle from obese people before and after Roux-en-Y gastric bypass (RYGB). Obesity was associated with altered expression of a subset of genes enriched in metabo...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.03.018
更新日期:2013-04-25 00:00:00
abstract::The large number of mutations identified across all cancers represents an untapped reservoir of targets that can be useful for therapeutic targeting if highly selective, mutation-specific reagents are available. We report here our attempt to generate such reagents: monoclonal antibodies against the most common R175H, ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.11.112
更新日期:2018-01-02 00:00:00
abstract::Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates RB and functions as a collaborative nuclear oncogene. The serine threonine kinase Akt plays a pivotal role in the control of cellular metabolism, survival, and mitogenic signaling. Herein, Akt1-mediated phosphorylation of downstream substrate...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108151
更新日期:2020-09-15 00:00:00
abstract::Telomerase biogenesis is a complex process where several steps remain poorly understood. Single-strand-selective uracil-DNA glycosylase (SMUG1) associates with the DKC1-containing H/ACA ribonucleoprotein complex, which is essential for telomerase biogenesis. Herein, we show that SMUG1 interacts with the telomeric RNA ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.07.040
更新日期:2019-08-13 00:00:00
abstract::SUMOylation has been implicated in cellular stress adaptation, but its role in regulating liver kinase B1 (LKB1), a major upstream kinase of the energy sensor AMP-activated protein kinase (AMPK), is unknown. Here, we show that energy stress triggers an increase in SUMO1 modification of LKB1, despite a global reduction...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.07.002
更新日期:2015-08-04 00:00:00
abstract::Pneumonias caused by influenza A virus (IAV) co- and secondary bacterial infections are characterized by their severity and high mortality rate. Previously, we have shown that bacterial pore-forming toxin (PFT)-mediated necroptosis is a key driver of acute lung injury during bacterial pneumonia. Here, we evaluate the ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108062
更新日期:2020-08-25 00:00:00
abstract::Cell-cycle arrest in quiescence and senescence is largely orchestrated by the retinoblastoma (Rb) tumor-suppressor pathway, but the mechanisms underlying the quiescence-senescence switch remain unclear. Here, we show that the crosstalk between the Rb-AKT-signaling pathways forms this switch by controlling the overlapp...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.03.006
更新日期:2014-04-10 00:00:00
abstract::Memory B cells (MBCs) epitomize the adaptation of the immune system to the environment. We identify two MBC subsets in peripheral blood, CD27dull and CD27bright MBCs, whose frequency changes with age. Heavy chain variable region (VH) usage, somatic mutation frequency replacement-to-silent ratio, and CDR3 property chan...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.02.022
更新日期:2020-03-03 00:00:00
abstract::Autism spectrum disorders (ASDs) are associated with mutations affecting synaptic components, including GluN2B-NMDA receptors (NMDARs) and neurobeachin (NBEA). NBEA participates in biosynthetic pathways to regulate synapse receptor targeting, synaptic function, cognition, and social behavior. However, the role of NBEA...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.04.112
更新日期:2018-05-29 00:00:00
abstract::The rate at which genomes diverge during speciation is unknown, as are the physical dynamics of the process. Here, we compare full genome sequences of 32 butterflies, representing five species from a hybridizing Heliconius butterfly community, to examine genome-wide patterns of introgression and infer how divergence e...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.09.042
更新日期:2013-11-14 00:00:00
abstract::Dorsal root ganglia (DRG) relay sensory information to the brain, giving rise to the perception of pain, disorders of which are prevalent and burdensome. Here, we mapped expression quantitative trait loci (eQTLs) in a collection of human DRGs. DRG eQTLs were enriched within untranslated regions of coding genes of low ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.05.018
更新日期:2017-05-30 00:00:00