当前位置： SCI文献检索 > Cell Reports期刊下所有文献 > Early-Onset Hypertrophic Cardiomyopathy Mutations Significantly Increase the Velocity, Force, and Actin-Activated ATPase Activity of Human β-Cardiac Myosin.

Early-Onset Hypertrophic Cardiomyopathy Mutations Significantly Increase the Velocity, Force, and Actin-Activated ATPase Activity of Human β-Cardiac Myosin.

Abstract：

:Hypertrophic cardiomyopathy (HCM) is a heritable cardiovascular disorder that affects 1 in 500 people. A significant percentage of HCM is attributed to mutations in β-cardiac myosin, the motor protein that powers ventricular contraction. This study reports how two early-onset HCM mutations, D239N and H251N, affect the molecular biomechanics of human β-cardiac myosin. We observed significant increases (20%-90%) in actin gliding velocity, intrinsic force, and ATPase activity in comparison to wild-type myosin. Moreover, for H251N, we found significantly lower binding affinity between the S1 and S2 domains of myosin, suggesting that this mutation may further increase hyper-contractility by releasing active motors. Unlike previous HCM mutations studied at the molecular level using human β-cardiac myosin, early-onset HCM mutations lead to significantly larger changes in the fundamental biomechanical parameters and show clear hyper-contractility.

journal_name

Cell Rep

journal_title

Cell reports

authors

Adhikari AS,Kooiker KB,Sarkar SS,Liu C,Bernstein D,Spudich JA,Ruppel KM

doi

10.1016/j.celrep.2016.11.040

subject

Has Abstract

pub_date

2016-12-13 00:00:00

pages

2857-2864

issue

11

issn

2211-1247

pii

S2211-1247(16)31598-4

journal_volume

17

pub_type

杂志文章

相关文献

Cell Reports文献大全
  • mTORC1 Signaling Controls TLR2-Mediated T-Cell Activation by Inducing TIRAP Expression.

    abstract：:Effector, but not naïve, T cells are activated by toll-like receptor-2 (TLR2) stimulation, leading to cytokine production and proliferation. We found that the differential response is attributable to the lack of expression of the adaptor protein TIRAP in naive T cells. TIRAP expression is induced upon T-cell receptor ...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.107911

    authors： Imanishi T,Unno M,Kobayashi W,Yoneda N,Akira S,Saito T

    更新日期：2020-07-21 00:00:00

  • Microglial Displacement of GABAergic Synapses Is a Protective Event during Complex Febrile Seizures.

    abstract：:Complex febrile seizures (FSs) lead to a high risk of intractable temporal lobe epilepsy during adulthood, yet the pathological process of complex FSs is largely unknown. Here, we demonstrate that activated microglia extensively associated with glutamatergic neuronal soma displace surrounding GABAergic presynapses in ...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.108346

    authors： Wan Y,Feng B,You Y,Yu J,Xu C,Dai H,Trapp BD,Shi P,Chen Z,Hu W

    更新日期：2020-11-03 00:00:00

  • SNAREs Controlling Vesicular Release of BDNF and Development of Callosal Axons.

    abstract：:At presynaptic active zones, exocytosis of neurotransmitter vesicles (SVs) is driven by SNARE complexes that recruit Syb2 and SNAP25. However, it remains unknown which SNAREs promote the secretion of neuronal proteins, including those essential for circuit development and experience-dependent plasticity. Here we demon...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2015.04.032

    authors： Shimojo M,Courchet J,Pieraut S,Torabi-Rander N,Sando R 3rd,Polleux F,Maximov A

    更新日期：2015-05-19 00:00:00

  • Elucidating MicroRNA Regulatory Networks Using Transcriptional, Post-transcriptional, and Histone Modification Measurements.

    abstract：:MicroRNAs (miRNAs) regulate diverse biological processes by repressing mRNAs, but their modest effects on direct targets, together with their participation in larger regulatory networks, make it challenging to delineate miRNA-mediated effects. Here, we describe an approach to characterizing miRNA-regulatory networks b...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2015.12.031

    authors： Gosline SJ,Gurtan AM,JnBaptiste CK,Bosson A,Milani P,Dalin S,Matthews BJ,Yap YS,Sharp PA,Fraenkel E

    更新日期：2016-01-12 00:00:00

  • An Antifungal Combination Matrix Identifies a Rich Pool of Adjuvant Molecules that Enhance Drug Activity against Diverse Fungal Pathogens.

    abstract：:There is an urgent need to identify new treatments for fungal infections. By combining sub-lethal concentrations of the known antifungals fluconazole, caspofungin, amphotericin B, terbinafine, benomyl, and cyprodinil with ∼3,600 compounds in diverse fungal species, we generated a deep reservoir of chemical-chemical in...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2015.10.018

    authors： Robbins N,Spitzer M,Yu T,Cerone RP,Averette AK,Bahn YS,Heitman J,Sheppard DC,Tyers M,Wright GD

    更新日期：2015-11-17 00:00:00

  • FOXK1 Participates in DNA Damage Response by Controlling 53BP1 Function.

    abstract：:53BP1 plays a central role in dictating DNA repair choice between non-homologous end joining (NHEJ) and homologous recombination (HR), which is important for the sensitivity to poly(ADP-ribose) polymerase inhibitors (PARPis) of BRCA1-deficient cancers. In this study, we show that FOXK1 associates with 53BP1 and regula...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.108018

    authors： Tang M,Feng X,Pei G,Srivastava M,Wang C,Chen Z,Li S,Zhang H,Zhao Z,Li X,Chen J

    更新日期：2020-08-11 00:00:00

  • The Self-Inactivating KamiCas9 System for the Editing of CNS Disease Genes.

    abstract：:Neurodegenerative disorders are a major public health problem because of the high frequency of these diseases. Genome editing with the CRISPR/Cas9 system is making it possible to modify the sequence of genes linked to these disorders. We designed the KamiCas9 self-inactivating editing system to achieve transient expre...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.08.075

    authors： Merienne N,Vachey G,de Longprez L,Meunier C,Zimmer V,Perriard G,Canales M,Mathias A,Herrgott L,Beltraminelli T,Maulet A,Dequesne T,Pythoud C,Rey M,Pellerin L,Brouillet E,Perrier AL,du Pasquier R,Déglon N

    更新日期：2017-09-19 00:00:00

  • Tumor-Associated Macrophages Suppress the Cytotoxic Activity of Antimitotic Agents.

    abstract：:Antimitotic agents, including Taxol, disrupt microtubule dynamics and cause a protracted mitotic arrest and subsequent cell death. Despite the broad utility of these drugs in breast cancer and other tumor types, clinical response remains variable. Tumor-associated macrophages (TAMs) suppress the duration of Taxol-indu...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.03.038

    authors： Olson OC,Kim H,Quail DF,Foley EA,Joyce JA

    更新日期：2017-04-04 00:00:00

  • Regulation of EZH2 by SMYD2-Mediated Lysine Methylation Is Implicated in Tumorigenesis.

    abstract：:The histone methyl transferase enhancer of zeste homolog 2 (EZH2) is a master transcriptional regulator involved in histone H3 lysine 27 trimethylation. We aimed to elucidate the precise post-translational regulations of EZH2 and their role in cancer pathogenesis. Here, we show that SET and MYND domain containing 2 (S...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.10.004

    authors： Zeng Y,Qiu R,Yang Y,Gao T,Zheng Y,Huang W,Gao J,Zhang K,Liu R,Wang S,Hou Y,Yu W,Leng S,Feng D,Liu W,Zhang X,Wang Y

    更新日期：2019-11-05 00:00:00

  • The GLP1R Agonist Liraglutide Reduces Hyperglucagonemia Induced by the SGLT2 Inhibitor Dapagliflozin via Somatostatin Release.

    abstract：:The newest classes of anti-diabetic agents include sodium-glucose cotransporter 2 (SGLT2) inhibitors and glucagon-like peptide 1 receptor (GLP1R) agonists. The SGLT2 inhibitor dapagliflozin reduces glucotoxicity by glycosuria but elevates glucagon secretion. The GLP1R agonist liraglutide inhibits glucagon; therefore, ...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.07.009

    authors： Saponaro C,Gmyr V,Thévenet J,Moerman E,Delalleau N,Pasquetti G,Coddeville A,Quenon A,Daoudi M,Hubert T,Vantyghem MC,Bousquet C,Martineau Y,Kerr-Conte J,Staels B,Pattou F,Bonner C

    更新日期：2019-08-06 00:00:00

  • Vitamin D Receptor Controls Cell Stemness in Acute Myeloid Leukemia and in Normal Bone Marrow.

    abstract：:Vitamin D (VD) is a known differentiating agent, but the role of VD receptor (VDR) is still incompletely described in acute myeloid leukemia (AML), whose treatment is based mostly on antimitotic chemotherapy. Here, we present an unexpected role of VDR in normal hematopoiesis and in leukemogenesis. Limited VDR expressi...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.12.055

    authors： Paubelle E,Zylbersztejn F,Maciel TT,Carvalho C,Mupo A,Cheok M,Lieben L,Sujobert P,Decroocq J,Yokoyama A,Asnafi V,Macintyre E,Tamburini J,Bardet V,Castaigne S,Preudhomme C,Dombret H,Carmeliet G,Bouscary D,Ginzburg YZ

    更新日期：2020-01-21 00:00:00

  • A Transcriptomic Signature of the Hypothalamic Response to Fasting and BDNF Deficiency in Prader-Willi Syndrome.

    abstract：:Transcriptional analysis of brain tissue from people with molecularly defined causes of obesity may highlight disease mechanisms and therapeutic targets. We performed RNA sequencing of hypothalamus from individuals with Prader-Willi syndrome (PWS), a genetic obesity syndrome characterized by severe hyperphagia. We fou...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2018.03.018

    authors： Bochukova EG,Lawler K,Croizier S,Keogh JM,Patel N,Strohbehn G,Lo KK,Humphrey J,Hokken-Koelega A,Damen L,Donze S,Bouret SG,Plagnol V,Farooqi IS

    更新日期：2018-03-27 00:00:00

  • From Retina to Stem Cell and Back Again: Memories of a Chromatin Journey.

    abstract：:The use of retinal organoids requires efficient differentiation from induced pluripotent stem cells (iPSCs). In this issue of Cell Reports, Wang et al. (2018) examine how the chromatin landscape after iPSC programming predicts their ability to differentiate into retinal tissue. ...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2018.02.078

    authors： Roberts JM,Vetter ML

    更新日期：2018-03-06 00:00:00

  • DYRK1A Kinase Positively Regulates Angiogenic Responses in Endothelial Cells.

    abstract：:Angiogenesis is a highly regulated process essential for organ development and maintenance, and its deregulation contributes to inflammation, cardiac disorders, and cancer. The Ca2+/nuclear factor of activated T cells (NFAT) signaling pathway is central to endothelial cell angiogenic responses, and it is activated by ...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2018.04.008

    authors： Rozen EJ,Roewenstrunk J,Barallobre MJ,Di Vona C,Jung C,Figueiredo AF,Luna J,Fillat C,Arbonés ML,Graupera M,Valverde MA,de la Luna S

    更新日期：2018-05-08 00:00:00

  • Pericytes Stimulate Oligodendrocyte Progenitor Cell Differentiation during CNS Remyelination.

    abstract：:The role of the neurovascular niche in CNS myelin regeneration is incompletely understood. Here, we show that, upon demyelination, CNS-resident pericytes (PCs) proliferate, and parenchymal non-vessel-associated PC-like cells (PLCs) rapidly develop. During remyelination, mature oligodendrocytes were found in close prox...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.08.007

    authors： De La Fuente AG,Lange S,Silva ME,Gonzalez GA,Tempfer H,van Wijngaarden P,Zhao C,Di Canio L,Trost A,Bieler L,Zaunmair P,Rotheneichner P,O'Sullivan A,Couillard-Despres S,Errea O,Mäe MA,Andrae J,He L,Keller A,Bátiz LF

    更新日期：2017-08-22 00:00:00

  • A Defective mRNA Cleavage and Polyadenylation Complex Facilitates Expansions of Transcribed (GAA)n Repeats Associated with Friedreich's Ataxia.

    abstract：:Expansions of microsatellite repeats are responsible for numerous hereditary diseases in humans, including myotonic dystrophy and Friedreich's ataxia. Whereas the length of an expandable repeat is the main factor determining disease inheritance, recent data point to genomic trans modifiers that can impact the likeliho...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.08.051

    authors： McGinty RJ,Puleo F,Aksenova AY,Hisey JA,Shishkin AA,Pearson EL,Wang ET,Housman DE,Moore C,Mirkin SM

    更新日期：2017-09-05 00:00:00

  • Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms.

    abstract：:Desensitization, signaling, and trafficking of G-protein-coupled receptors (GPCRs) are critically regulated by multifunctional adaptor proteins, β-arrestins (βarrs). The two isoforms of βarrs (βarr1 and 2) share a high degree of sequence and structural similarity; still, however, they often mediate distinct functional...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.08.053

    authors： Ghosh E,Dwivedi H,Baidya M,Srivastava A,Kumari P,Stepniewski T,Kim HR,Lee MH,van Gastel J,Chaturvedi M,Roy D,Pandey S,Maharana J,Guixà-González R,Luttrell LM,Chung KY,Dutta S,Selent J,Shukla AK

    更新日期：2019-09-24 00:00:00

  • Clarifying the Translational Pausing Landscape in Bacteria by Ribosome Profiling.

    abstract：:The rate of protein synthesis varies according to the mRNA sequence in ways that affect gene expression. Global analysis of translational pausing is now possible with ribosome profiling. Here, we revisit an earlier report that Shine-Dalgarno sequences are the major determinant of translational pausing in bacteria. Usi...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2015.12.073

    authors： Mohammad F,Woolstenhulme CJ,Green R,Buskirk AR

    更新日期：2016-02-02 00:00:00

  • Innate Lymphoid Cells in the Induction of Obesity.

    abstract：:Complex interactions between immune cells are an important component in the induction of obesity. Here, we show that Il2rg-/-Rag2-/- mice lacking all lymphocytes are resistant to diet-induced obesity. Transplantation of bone marrow cells from Rag2-/- mice, which lack only acquired immune cells, into Il2rg-/-Rag2-/- mi...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.06.016

    authors： Sasaki T,Moro K,Kubota T,Kubota N,Kato T,Ohno H,Nakae S,Saito H,Koyasu S

    更新日期：2019-07-02 00:00:00

  • In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9.

    abstract：:CRISPR/Cas-based technologies have revolutionized genetic approaches to addressing a wide range of neurobiological questions. The ability of CRISPR/Cas to introduce mutations into target genes allows us to perform in vivo loss-of-function experiments without generating genetically engineered mice. However, the lack of...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.06.038

    authors： Steinecke A,Kurabayashi N,Hayano Y,Ishino Y,Taniguchi H

    更新日期：2019-07-09 00:00:00

  • Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity.

    abstract：:The quality of mitochondria in skeletal muscle is essential for maintaining metabolic homeostasis during adaptive stress responses. However, the precise control mechanism of muscle mitochondrial quality and its physiological impacts remain unclear. Here, we demonstrate that FUNDC1, a mediator of mitophagy, plays a cri...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2018.03.127

    authors： Fu T,Xu Z,Liu L,Guo Q,Wu H,Liang X,Zhou D,Xiao L,Liu L,Liu Y,Zhu MS,Chen Q,Gan Z

    更新日期：2018-05-01 00:00:00

  • Agrin as a Mechanotransduction Signal Regulating YAP through the Hippo Pathway.

    abstract：:The Hippo pathway effectors YAP and TAZ act as nuclear sensors of mechanical signals in response to extracellular matrix (ECM) cues. However, the identity and nature of regulators in the ECM and the precise pathways relaying mechanoresponsive signals into intracellular sensors remain unclear. Here, we uncover a functi...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.02.041

    authors： Chakraborty S,Njah K,Pobbati AV,Lim YB,Raju A,Lakshmanan M,Tergaonkar V,Lim CT,Hong W

    更新日期：2017-03-07 00:00:00

  • Overexpression of Trophoblast Stem Cell-Enriched MicroRNAs Promotes Trophoblast Fate in Embryonic Stem Cells.

    abstract：:The first cell fate choice of the preimplantation embryo generates the extraembryonic trophoblast and embryonic epiblast lineages. Embryonic stem cells (ESCs) and trophoblast stem cells (TSCs) can be utilized to investigate molecular mechanisms of this first cell fate decision. It has been established that ESCs can be...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.04.040

    authors： Nosi U,Lanner F,Huang T,Cox B

    更新日期：2017-05-09 00:00:00

  • DNA-PK Is Targeted by Multiple Vaccinia Virus Proteins to Inhibit DNA Sensing.

    abstract：:Virus infection is sensed by pattern recognition receptors (PRRs) detecting virus nucleic acids and initiating an innate immune response. DNA-dependent protein kinase (DNA-PK) is a PRR that binds cytosolic DNA and is antagonized by vaccinia virus (VACV) protein C16. Here, VACV protein C4 is also shown to antagonize DN...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2018.10.034

    authors： Scutts SR,Ember SW,Ren H,Ye C,Lovejoy CA,Mazzon M,Veyer DL,Sumner RP,Smith GL

    更新日期：2018-11-13 00:00:00

  • Downregulation of a GPCR by β-Arrestin2-Mediated Switch from an Endosomal to a TGN Recycling Pathway.

    abstract：:Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone involved in nutrient homeostasis. GIP receptor (GIPR) is constitutively internalized and returned to the plasma membrane, atypical behavior for a G-protein-coupled receptor (GPCR). GIP promotes GIPR downregulation from the plasma membrane by inh...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2016.11.050

    authors： Abdullah N,Beg M,Soares D,Dittman JS,McGraw TE

    更新日期：2016-12-13 00:00:00

  • Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions.

    abstract：:After implantation, pluripotent epiblasts are converted to embryonic ectoderm through cell-cell interactions that significantly change the transcriptional and epigenetic networks. An entrée to understanding this vital developmental transition is the t(w5) mutation of the mouse t complex. This mutation produces highly ...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2012.10.004

    authors： Sugimoto M,Kondo M,Hirose M,Suzuki M,Mekada K,Abe T,Kiyonari H,Ogura A,Takagi N,Artzt K,Abe K

    更新日期：2012-11-29 00:00:00

  • Systematic Characterization of Recurrent Genomic Alterations in Cyclin-Dependent Kinases Reveals Potential Therapeutic Strategies for Cancer Treatment.

    abstract：:Recurrent copy-number alterations, mutations, and transcript fusions of the genes encoding CDKs/cyclins are characterized in >10,000 tumors. Genomic alterations of CDKs/cyclins are dominantly driven by copy number aberrations. In contrast to cell-cycle-related CDKs/cyclins, which are globally amplified, transcriptiona...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.107884

    authors： Shan W,Yuan J,Hu Z,Jiang J,Wang Y,Loo N,Fan L,Tang Z,Zhang T,Xu M,Pan Y,Lu J,Long M,Tanyi JL,Montone KT,Fan Y,Hu X,Zhang Y,Zhang L

    更新日期：2020-07-14 00:00:00

  • Three Types of Functional Regulatory T Cells Control T Cell Responses at the Human Maternal-Fetal Interface.

    abstract：:During pregnancy, maternal regulatory T cells (Tregs) are important in establishing immune tolerance to invading fetal extravillous trophoblasts (EVTs). CD25HIFOXP3+ Tregs are found at high levels in decidual tissues and have been shown to suppress fetus-specific and nonspecific responses. However, limited data are av...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.04.109

    authors： Salvany-Celades M,van der Zwan A,Benner M,Setrajcic-Dragos V,Bougleux Gomes HA,Iyer V,Norwitz ER,Strominger JL,Tilburgs T

    更新日期：2019-05-28 00:00:00

  • Aβ secretion and plaque formation depend on autophagy.

    abstract：:Alzheimer's disease (AD) is a neurodegenerative disease biochemically characterized by aberrant protein aggregation, including amyloid beta (Aβ) peptide accumulation. Protein aggregates in the cell are cleared by autophagy, a mechanism impaired in AD. To investigate the role of autophagy in Aβ pathology in vivo, we cr...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2013.08.042

    authors： Nilsson P,Loganathan K,Sekiguchi M,Matsuba Y,Hui K,Tsubuki S,Tanaka M,Iwata N,Saito T,Saido TC

    更新日期：2013-10-17 00:00:00

  • Targeted Neurostimulation in Mouse Brains with Non-invasive Ultrasound.

    abstract：:Recently developed brain stimulation techniques have significantly advanced our ability to manipulate the brain's function. However, stimulating specific neurons in a desired region without significant surgical invasion remains a challenge. Here, we demonstrate a neuron-specific and region-targeted neural excitation s...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.108033

    authors： Qiu Z,Kala S,Guo J,Xian Q,Zhu J,Zhu T,Hou X,Wong KF,Yang M,Wang H,Sun L

    更新日期：2020-08-18 00:00:00