Abstract：
:Protein disulfide isomerases (PDI) are a family of redox chaperones that catalyze formation or isomerization of disulfide bonds in proteins. Previous studies have shown that one member, PDIA3, interacts with influenza A virus (IAV) hemagglutinin (HA), and this interaction is required for efficient oxidative folding of HA in vitro. However, it is unknown whether these host-viral protein interactions occur during active infection and whether such interactions represent a putative target for the treatment of influenza infection. Here we show that PDIA3 is specifically upregulated in IAV-infected mouse or human lung epithelial cells and PDIA3 directly interacts with IAV-HA. Treatment with a PDI inhibitor, LOC14 inhibited PDIA3 activity in lung epithelial cells, decreased intramolecular disulfide bonds and subsequent oligomerization (maturation) of HA in both H1N1 (A/PR8/34) and H3N2 (X31, A/Aichi/68) infected lung epithelial cells. These reduced disulfide bond formation significantly decreased viral burden, and also pro-inflammatory responses from lung epithelial cells. Lung epithelial-specific deletion of PDIA3 in mice resulted in a significant decrease in viral burden and lung inflammatory-immune markers upon IAV infection, as well as significantly improved airway mechanics. Taken together, these results indicate that PDIA3 is required for effective influenza pathogenesis in vivo, and pharmacological inhibition of PDIs represents a promising new anti-influenza therapeutic strategy during pandemic and severe influenza seasons.
journal_name
Redox Bioljournal_title
Redox biologyauthors
Chamberlain N,Korwin-Mihavics BR,Nakada EM,Bruno SR,Heppner DE,Chapman DG,Hoffman SM,van der Vliet A,Suratt BT,Dienz O,Alcorn JF,Anathy Vdoi
10.1016/j.redox.2019.101129subject
Has Abstractpub_date
2019-04-01 00:00:00pages
101129issn
2213-2317pii
S2213-2317(18)31228-Xjournal_volume
22pub_type
杂志文章相关文献
Redox Biology文献大全abstract：:Exposure to the space radiation environment poses risks for a range of deleterious health effects due to the unique types of radiation encountered. Galactic cosmic rays are comprised of a spectrum of highly energetic nuclei that deposit densely ionizing tracks of damage along the particle trajectory. These tracks are ...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2015.03.001
更新日期：2015-08-01 00:00:00
abstract：:Culturing cells and tissues in vitro has provided valuable insights into the molecular mechanisms regulating redox signaling in cells with implications for medicine. However, standard culture techniques maintain mammalian cells in vitro under an artificial physicochemical environment such as ambient air and 5% CO2. Ox...
journal_title：Redox biology
pub_type： 杂志文章,评审
doi：10.1016/j.redox.2014.04.008
更新日期：2014-04-18 00:00:00
abstract：:Hydroxytyrosol (HT) is a main phenolic component of olive oil. In this study, we investigated the safety and effects produced by HT purified (99.5%) from olive mill waste. HT was administered at a daily dosage of 45mg for 8 weeks to volunteers with mild hyperlipidemia (n=14). We measured markers of cardiovascular dise...
journal_title：Redox biology
pub_type： 临床试验,杂志文章
doi：10.1016/j.redox.2016.12.014
更新日期：2017-04-01 00:00:00
abstract：:Cell-free heme (CFH) and hemoglobin (Hb) have emerged as distinct mediators of acute injury characterized by inflammation and microcirculatory dysfunction in hemolytic conditions and critical illness. Several reports have shown changes in Hb and CFH in specific pathophysiological settings. Using PBS, plasma from patie...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2016.08.003
更新日期：2016-10-01 00:00:00
abstract：:Williamson and colleagues present important data on the effects of MitoQ - an antioxidant compound targeted to mitochondria - on mtDNA damage following exercise. Future studies are needed to elucidate, whether or not the observed prevention of MitoQ on DNA damage is beneficial with regard to functional outcomes in hea...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2020.101732
更新日期：2021-01-01 00:00:00
abstract：:In this paper, we describe an assay to analyze simultaneously the oxygen consumption rate (OCR) and superoxide production in a biological system. The analytical set-up uses electron paramagnetic resonance (EPR) spectroscopy with two different isotopically-labelled sensors: 15N-PDT (4-oxo-2,2,6,6-tetramethylpiperidine-...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2020.101852
更新日期：2020-12-28 00:00:00
abstract：:Obesity and excessive inflammation/oxidative stress are pathophysiological forces associated with kidney dysfunction. Although we recently showed that heme-oxygenase (HO) improves renal functions, the mechanisms are largely unclear. Moreover, the effects of the HO-system on podocyte cytoskeletal proteins like podocin,...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2014.09.001
更新日期：2014-01-01 00:00:00
abstract：:Acute oxygen (O2) sensing is essential for individuals to survive under hypoxic conditions. The carotid body (CB) is the main peripheral chemoreceptor, which contains excitable and O2-sensitive glomus cells with O2-regulated ion channels. Upon exposure to acute hypoxia, inhibition of K+ channels is the signal that tri...
journal_title：Redox biology
pub_type： 杂志文章,评审
doi：10.1016/j.redox.2017.04.033
更新日期：2017-08-01 00:00:00
abstract：:Fanconi Anemia (FA) is a disease characterized by bone marrow (BM) failure and aplastic anemia. In addition to a defective DNA repair system, other mechanisms are involved in its pathogenesis, such as defective mitochondrial metabolism, accumulation of lipids, and increment of oxidative stress production. To better un...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2020.101618
更新日期：2020-09-01 00:00:00
abstract：:The generation of excessive amounts of reactive oxygen species (ROS) leads to cellular oxidative stress that underlies a variety of forms of hepatocyte injury and death including that from alcohol. Although ROS can induce cell damage through direct effects on cellular macromolecules, the injurious effects of ROS are m...
journal_title：Redox biology
pub_type： 杂志文章,评审
doi：10.1016/j.redox.2014.09.004
更新日期：2014-01-01 00:00:00
abstract：:Hydrogen peroxide (H2O2) is an essential second intracellular messenger. To reach its targets in the cytosol, H2O2 must cross a membrane, a feat that requires aquaporins (AQP) endowed with 'peroxiporin' activity (AQP3, AQP8, AQP9). Here, we exploit different organelle-targeted H2O2-sensitive probes to show that also A...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2019.101326
更新日期：2020-01-01 00:00:00
abstract：:In the present study, we aimed to investigate the impact of permanent cystathionine-β-Synthase (CBS) gene knockdown in human telomerase reverse transcriptase (hTERT) immortalized human adipose-derived mesenchymal stem cells (ASC52telo) and in their capacity to differentiate into adipocytes. CBS gene KD in ASC52telo ce...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2020.101668
更新日期：2020-08-02 00:00:00
abstract：BACKGROUND:Macrophages regulate the inflammatory response and affect re-endothelialization. Inflammation and macrophages play important roles in promoting tissue repair, but p38α mitogen-activated protein kinase's role in re-endothelialization is unknown. METHODS AND RESULTS:Wire injuries of carotid arteries and Evans...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2020.101775
更新日期：2021-01-01 00:00:00
abstract：:Sulfane sulfur is common inside cells, playing both regulatory and antioxidant roles. However, there are unresolved issues about its chemistry and biochemistry. We report the discovery that reactive sulfane sulfur such as polysulfides and persulfides could be detected by using resonance synchronous spectroscopy (RS2)....
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2019.101179
更新日期：2019-06-01 00:00:00
abstract：:Hypertension is one of the major predisposing factors for neurodegenerative disease characterized with activated renin-angiotensin system (RAS) in both periphery and brain. Vitamin D (VitD) is recently recognized as a pleiotropic hormone with strong neuroprotective properties. While multiple lines of evidence suggest ...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2019.101295
更新日期：2019-09-01 00:00:00
abstract：:Fibrosis is one of the most prevalent features of age-related diseases like obesity, diabetes, non-alcoholic fatty liver disease, chronic kidney disease, or cardiomyopathy and affects millions of people in all countries. Although the understanding about the pathophysiology of fibrosis has improved a lot during the rec...
journal_title：Redox biology
pub_type： 杂志文章,评审
doi：10.1016/j.redox.2015.08.015
更新日期：2015-12-01 00:00:00
abstract：:Oxidative stress response in bacteria is mediated through coordination between the regulators of oxidant-remediation systems (e.g. OxyR, SoxR) and nucleoid condensation (e.g. Dps, Fis). However, these genetic factors are either absent or rendered non-functional in the human pathogen Mycobacterium tuberculosis (Mtb). T...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2018.08.006
更新日期：2018-10-01 00:00:00
abstract：:Ferroptosis is an outcome of metabolic disorders and closely linked to liver cancer. However, the mechanism underlying the fine regulation of ferroptosis in liver cancer remains unclear. Here, we have identified two categories of genes: ferroptosis up-regulated factors (FUF) and ferroptosis down-regulated factors (FDF...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2019.101211
更新日期：2019-06-01 00:00:00
abstract：:Hypertonia is pathognomonic of cerebral palsy (CP), often caused by brain injury before birth. To understand the early driving events of hypertonia, we utilized magnetic resonance imaging (MRI) assessment of early critical brain injury in rabbit fetuses (79% term) that will predict hypertonia after birth following ant...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2019.101407
更新日期：2020-01-01 00:00:00
abstract：:The development of hypertension is associated with mitochondrial redox balance disruptions. NADP+-dependent isocitrate dehydrogenase 2 (IDH2) plays an important role in the maintenance of mitochondrial redox balance by producing mitochondrial NADPH, which is an essential cofactor in the reduction of glutathione (from ...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2020.101548
更新日期：2020-07-01 00:00:00
abstract：:Hydrogen sulfide (H2S) is a gaseous signalling molecule involved in many physiological and pathological processes. There is increasing evidence that H2S is implicated in aging and lifespan control in the diet-induced longevity models. However, blood sulfide concentration of naturally long-lived species is not known. H...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2016.01.008
更新日期：2016-08-01 00:00:00
abstract：:Calcium (Ca2+) and reactive oxygen species (ROS) are versatile signaling molecules coordinating physiological and pathophysiological processes. While channels and pumps shuttle Ca2+ ions between extracellular space, cytosol and cellular compartments, short-lived and highly reactive ROS are constantly generated by vari...
journal_title：Redox biology
pub_type： 杂志文章,评审
doi：10.1016/j.redox.2020.101678
更新日期：2020-09-01 00:00:00
abstract：:The biological functions of N6-methyladenosine (m6A) RNA methylation are mainly dependent on the reader; however, its role in lung tumorigenesis remains unclear. Here, we have demonstrated that the m6A reader YT521-B homology domain containing 2 (YTHDC2) is frequently suppressed in lung adenocarcinoma (LUAD). Downregu...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2020.101801
更新日期：2021-01-01 00:00:00
abstract：:The NADPH oxidase reaction produces protons. In the case of the NADPH oxidase, NOX2, activity depends on secretion of these protons and is inhibited by blockade of the voltage-gated proton channel (Hv1). Duox1 and Duox2 activities similarly produce intracellular protons but synthesize hydrogen peroxide directly instea...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2019.101346
更新日期：2020-01-01 00:00:00
abstract：:Glucose starvation is one of the major forms of metabolic stress in cancer cells. Deprivation of glucose impairs glycolysis and the pentose phosphate pathway, which elicits oxidative stress due to enhanced production of reactive oxygen species (ROS) and impaired antioxidant system, leading to redox imbalance and cell ...
journal_title：Redox biology
pub_type： 杂志文章,评审
doi：10.1016/j.redox.2019.101154
更新日期：2019-07-01 00:00:00
abstract：:Macrophage recruitment and pro-inflammatory differentiation are hallmarks of various diseases, including infection and sepsis. Although studies suggest that mitochondria may regulate macrophage immune responses, it remains unclear whether mitochondrial mass affects macrophage pro-inflammatory differentiation. Here, we...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2020.101761
更新日期：2020-10-01 00:00:00
abstract：:Transcription factor Nuclear factor erythroid 2-related factor 2 (Nrf2) and its negative regulator, the E3 ligase adaptor Kelch-like ECH-associated protein 1 (Keap1), control the redox and metabolic homeostasis and oxidative stress. Inhibitors of Keap1-Nrf2 interaction are promising in oxidative stress related inflamm...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2020.101565
更新日期：2020-07-01 00:00:00
abstract：:Artesunate, an anti-malarial drug, has been repurposed as an anticancer drug due to its induction of cell death via reactive oxygen species (ROS) production. However, the molecular mechanisms regulating cancer cell death and the resistance of cells to artesunate remain unclear. We investigated the molecular mechanisms...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2016.12.010
更新日期：2017-04-01 00:00:00
abstract：:The generation of NO by the various NO synthases in normal and malignant tissues is manifested by various biological effects that are involved in the regulation of cell survival, differentiation and cell death. The role of NO in the cytotoxic immune response was first revealed by demonstrating the induction of iNOS in...
journal_title：Redox biology
pub_type： 杂志文章,评审
doi：10.1016/j.redox.2015.08.013
更新日期：2015-12-01 00:00:00
abstract：:Epidemiological studies have shown that estrogens have protective effects in cardiovascular diseases, even though the results from human clinical trials remain controversial, while most of the animal experiments confirmed this effect, but the detailed mechanism remains unclear. In this study, we found that estradiol (...
journal_title：Redox biology
pub_type： 杂志文章
doi：10.1016/j.redox.2014.11.001
更新日期：2014-01-01 00:00:00
