Staphylococcus aureus from atopic dermatitis skin accumulates in the lysosomes of keratinocytes with induction of IL-1α secretion via TLR9.


BACKGROUND:Staphylococcus aureus (S. aureus) is frequently detected in the skin of patients with atopic dermatitis (AD), and involved in the flare of AD. There are some evidence-specific strains of S. aureus affect the severity of AD. However, the mechanism of predominant colonization and the aggravation of dermatitis by certain strains of S. aureus in the patients with AD are still unknown. OBJECTIVE:To reveal the characteristics of S. aureus from patients with AD (S. aureus-AD), we analyzed the interaction of S. aureus-AD and keratinocytes in comparison with those of S. aureus laboratory strains (S. aureus-stand.). METHODS:We stimulated HaCaT cells, keratinocyte cell line, and human epidermal keratinocytes by heat-killed S. aureus strains, then evaluated immune response of keratinocytes by ELISA, immunofluorescence staining, and flow cytometry. RESULTS:Upon incubation with keratinocytes, three out of four strains of heat-killed S. aureus-AD were strongly agglutinated inside the cytoplasm. In the cells, they are located in lysosomes and promoted the secretion of interleukin-1α (IL-1α). These reactions were not observed by any of four strains of S. aureus-stand. and S. epidermidis and were abolished by the treatment of S. aureus with proteinase K. Moreover, the IL-1α secretion was diminished by the inhibition of Toll-like receptor 9 (TLR9). CONCLUSION:S. aureus-AD accumulates in lysosome of keratinocytes by means of bacterial cell wall proteins and induces IL-1α via TLR9.






Moriwaki M,Iwamoto K,Niitsu Y,Matsushima A,Yanase Y,Hisatsune J,Sugai M,Hide M




Has Abstract


2019-03-01 00:00:00














  • Leukocyte migration inhibition test in children with cow milk allergy.

    abstract::The usefulness of the leukocyte migration inhibition factor (LIF) test to detect cow milk (CM) hypersensitivity was studied in 40 children with suspected allergy to CM. Hypersensitivity was carefully investigated by oral milk challenges, which gave a final confirmation of cow milk hypersensitivity in 12 subjects, and ...


    pub_type: 杂志文章


    authors: Vanto T,Smogorzewska EM,Viander M,Kalimo K,Koivikko A

    更新日期:1987-11-01 00:00:00

  • Aspirin tolerance induced in aspirin-sensitive asthmatics.

    abstract::Twenty-nine patients with asthma and aspirin-sensitivity were studied in an attempt to induce tolerance to aspirin (ASA). Starting with the smallest ASA doses eliciting bronchial obstruction (threshold doses) we doubled the doses on subsequent days and finally achieved good tolerance of 600 mg ASA per day in 27 patien...


    pub_type: 杂志文章


    authors: Kowalski ML,Grzelewska-Rzymowska I,Rozniecki J,Szmidt M

    更新日期:1984-04-01 00:00:00

  • Oral allergy syndrome (OAS) in pollinosis patients after eating pistachio nuts: two cases with two different patterns of onset.

    abstract::We describe two uncommon cases of oral allergy syndrome (OAS) after eating pistachio nuts (p.n.) in subjects (a 54-year-old man and a 3-year-old girl) with exclusive skin prick test (SPT) positivity to Parietaria (P.) and pistachio nut (p.n.) allergens. Serologic P.- and p.n.-specific IgE determinations were also carr...


    pub_type: 杂志文章


    authors: Liccardi G,Mistrello G,Noschese P,Falagiani P,D'Amato M,D'Amato G

    更新日期:1996-12-01 00:00:00

  • Can anti-IgE therapy prevent airway remodeling in allergic asthma?

    abstract::Airway remodeling is a central feature of asthma. It is exemplified by thickening of the lamina reticularis and structural changes to the epithelium, submucosa, smooth muscle, and vasculature of the airway wall. Airway remodeling may result from persistent airway inflammation. Immunoglobulin E (IgE) is an important me...


    pub_type: 杂志文章,评审


    authors: Rabe KF,Calhoun WJ,Smith N,Jimenez P

    更新日期:2011-09-01 00:00:00

  • Airborne pollen levels and drug consumption for seasonal allergic rhinoconjunctivitis: a 10-year study in France.

    abstract:BACKGROUND:Few time-series studies, and none lasting longer than 4 years, have investigated the etiology of treated seasonal allergic rhinoconjunctivitis (SAR) on the basis of anti-allergic medication prescriptions. The aim of this article was to study the short-term relationship between pollen exposure and drug-treate...


    pub_type: 杂志文章


    authors: Caillaud DM,Martin S,Ségala C,Vidal P,Lecadet J,Pellier S,Rouzaire P,Tridon A,Evrard B

    更新日期:2015-01-01 00:00:00

  • Gastroesophageal reflux and asthma: a review of pathogenesis, diagnosis, and therapy.

    abstract::Gastroesophageal reflux disease (GERD) occurs in up to one-third of the adult US population. Most affected individuals are either unaware of their condition or do not seek medical help, relying on nonprescription acid suppressants and antacids for relief. GERD, a common disorder of infancy, old age, and pregnancy, is ...


    pub_type: 杂志文章,评审


    authors: Theodoropoulos DS,Lockey RF,Boyce HW Jr,Bukantz SC

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  • Clinical evaluation of in vitro leukocyte histamine release in allergy to muscle relaxant drugs.

    abstract::We have evaluated the in vitro leukocyte histamine release tests for the diagnosis of allergy to muscle relaxant drugs in 40 patients (Group A) and a control group of 44 subjects with negative leukocyte histamine release (Group B). Non-IgE dependent histamine release, expressed as a percentage of the total blood hista...


    pub_type: 杂志文章


    authors: Mata E,Guéant JL,Moneret-Vautrin DA,Bermejo N,Gérard P,Nicolas JP,Laxenaire MC

    更新日期:1992-10-01 00:00:00

  • Placebo-controlled challenges with perfume in patients with asthma-like symptoms.

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    pub_type: 临床试验,杂志文章,随机对照试验


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    更新日期:1996-06-01 00:00:00

  • Design, engineering and in vitro evaluation of MHC class-II targeting allergy vaccines.

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    pub_type: 杂志文章


    authors: Crameri R,Flückiger S,Daigle I,Kündig T,Rhyner C

    更新日期:2007-02-01 00:00:00

  • Rhinitis is an independent risk factor for developing cough apart from colds among adults.

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    pub_type: 杂志文章


    authors: Guerra S,Sherrill DL,Baldacci S,Carrozzi L,Pistelli F,Di Pede F,Viegi G

    更新日期:2005-03-01 00:00:00

  • Possible role of histamine in rheumatoid arthritis. Treatment with cimetidine and mepyramine.

    abstract::Basophilocytes from patients with rheumatoid arthritis (RA) responded to leukocyte nuclei from normal persons with histamine release; a similar histamine release induced by the nuclear components RNA and DNA has been demonstrated previously. A role of histamine in RA is also supported by the findings of clinical impro...


    pub_type: 杂志文章


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    更新日期:1981-08-01 00:00:00

  • Involvement of arachidonate cyclooxygenase products in bronchial hyperresponsiveness induced by subthreshold concentration of aerosolized thromboxane A2 analogue (STA2) in guinea pigs.

    abstract::Effects of a thromboxane synthetase inhibitor (OKY-046) and a cyclooxygenase inhibitor (indomethacin) on bronchial hyperresponsiveness induced by subthreshold concentration of aerosolized thromboxane A2 analogue (STA2) were investigated in anesthetized, artificially ventilated guinea pigs in order to examine the role ...


    pub_type: 杂志文章


    authors: Saito M,Fujimura M,Sakamoto S,Miyake Y,Shintani H,Yasui M,Matsuda T

    更新日期:1992-04-01 00:00:00

  • HLA DRB1*15-DPB1*05 haplotype: a susceptible gene marker for isocyanate-induced occupational asthma?

    abstract:BACKGROUND:There has been no study for evaluating the associations of human leukocyte antigen (HLA) class I and II alleles with toluene diisocyanate (TDI)-induced asthma in an Asian population. OBJECTIVE:The aim of this study was to investigate a susceptible or protective marker of HLA class I and II alleles in TDI-in...


    pub_type: 杂志文章


    authors: Kim SH,Oh HB,Lee KW,Shin ES,Kim CW,Hong CS,Nahm DH,Park HS

    更新日期:2006-07-01 00:00:00

  • A population-based epidemiologic analysis of deaths from anaphylaxis in Florida.

    abstract:BACKGROUND:Previous US population-based epidemiologic studies of anaphylactic deaths have been limited by small populations and/or few deaths. The objective of this study was to determine the 10-year incidence of death from anaphylaxis in Florida and its descriptive epidemiology. METHODS:Patients who died from anaphyl...


    pub_type: 杂志文章


    authors: Simon MR,Mulla ZD

    更新日期:2008-08-01 00:00:00

  • Skin prick test reactivity to common allergens in Finnish adolescents.

    abstract::We studied 708 adolescents aged 15-17 years in the 9th grade of school in Imatra. Of the eligible population born in 1962 77% were included. All were skin prick tested with 16 extracts from two manufacturers with 12 common allergens, which included pollens, epithelia, mite, house dust and fish. At least one positive, ...


    pub_type: 杂志文章


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    更新日期:1980-07-01 00:00:00

  • Elevated levels of peripheral-blood, naturally occurring aliphatic polyamines in bronchial asthmatic patients with active symptoms.

    abstract::The levels of peripheral-blood, naturally occurring aliphatic polyamines, such as putrescine, spermidine, and spermine, from 21 bronchial asthmatic patients (11 atopics and 10 nonatopics) were measured by postcolumn derivatization high-performance liquid chromatography analysis. None of the patients, except the 44-yea...


    pub_type: 杂志文章


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    更新日期:1992-12-01 00:00:00

  • Allergen-induced mediator release tests.

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    pub_type: 杂志文章,评审


    authors: Demoly P,Lebel B,Arnoux B

    更新日期:2003-07-01 00:00:00

  • Helminths can protect themselves against rejection inhibiting hostile respiratory allergy symptoms.

    abstract::The ability of common environmental allergens to stimulate IgE responses and thus to produce allergic diseases has tended to overshadow the fact that helminthic parasites are possibly the most potent inducers of this immunoglobulin that exists in nature. Although it has been well established that during these infectio...


    pub_type: 杂志文章,评审


    authors: Mingomataj EC,Xhixha F,Gjata E

    更新日期:2006-04-01 00:00:00

  • Influence of hyposensitization on soluble interleukin-2 receptor, eosinophil cationic protein, in vitro lymphocyte proliferation, in vitro lymphocyte adhesion, and lymphocyte membrane markers in childhood asthma.

    abstract::Soluble interleukin-2 receptor (sIL-2R), eosinophil cationic protein (ECP), the lymphoproliferative response to house-dust mite (HDM), adhesion to human umbilical vein endothelial cells (HUVEC), and lymphocyte membrane markers were studied in three groups of children: healthy children, asthmatic children without hypos...


    pub_type: 杂志文章


    authors: Moens MM,Van Bever HP,Stevens WJ,Mertens AV,Bridts CH,De Clerck LS

    更新日期:1994-09-01 00:00:00

  • The effect of immediate-hypersensitivity reactions on the level of SLPI, granulocyte elastase, alpha1-antitrypsin, and albumin in nasal secretions, by the method of unilateral antigen challenge.

    abstract:BACKGROUND:The aim of this paper was to investigate the role of SLPI in patients with allergic rhinitis. From this point of view, we also examined leukocyte elastase, alpha1-antitrypsin, and albumin. SLPI is an inhibitor of serine proteases such as leukocyte elastase, cathepsin G, and mast-cell chymase. Since chymase i...


    pub_type: 杂志文章


    authors: Westin U,Lundberg E,Wihl JA,Ohlsson K

    更新日期:1999-08-01 00:00:00

  • Latex allergy in Saudi children with spina bifida.

    abstract:BACKGROUND:Children with spina bifida (SB) are exposed to latex soon after birth during bladder catheterization, rectal disimpaction, and multiple surgical procedures. IgE-mediated latex-allergic reactions have been reported recently in these children. Our study was designed to assess the prevalence of allergic reactio...


    pub_type: 杂志文章


    authors: Kattan H,Harfi H,Tipirneni P

    更新日期:1999-01-01 00:00:00

  • Inhibition of leukotriene (SRS-A)-mediated acute lung anaphylaxis by azelastine in guinea pigs.

    abstract::Azelastine hydrochloride, chemically known as 1(2H)-phthalazinone, 4-[(4-chlorophenyl)methyl]-2-(hexahydro-1-methyl-1H-azepine-4-yl)-, monohydrochloride, is a novel, orally effective, long-acting, antiallergic/antiasthmatic agent. The ability of azelastine and selected antiallergic drugs to inhibit SRS-A (leukotriene)...


    pub_type: 杂志文章


    authors: Chand N,Nolan K,Diamantis W,Perhach JL Jr,Sofia RD

    更新日期:1986-09-01 00:00:00

  • The role of ORMDL proteins, guardians of cellular sphingolipids, in asthma.

    abstract::A family of widely expressed ORM-like (ORMDL) proteins has been recently linked to asthma in genomewide association studies in humans and extensively explored in in vivo studies in mice. ORMDL proteins are key regulators of serine palmitoyltransferase, an enzyme catalyzing the initial step of sphingolipid biosynthesis...


    pub_type: 杂志文章,评审


    authors: Paulenda T,Draber P

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  • A novel study design to investigate the early-life origins of asthma in children (SAGE study).

    abstract::This is a description of the Study of Asthma, Genes and the Environment (SAGE), a novel birth cohort created from provincial healthcare administrative records. It is a general population-based cohort, composed of children at high and low risk for asthma, living in urban and rural environments in Manitoba, Canada. The ...


    pub_type: 杂志文章


    authors: Kozyrskyj AL,HayGlass KT,Sandford AJ,Paré PD,Chan-Yeung M,Becker AB

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  • Comparision of two-flow cytometry methods for basophil degranulation in patients sensitized to grass pollen.

    abstract:BACKGROUND:Two-flow cytometry methods for quantification of degranulated basophil after allergen-specific activation were discussed. The methods are discerned by used membrane receptors--FcepsilonRI or IL-3Ralpha Our goal was to evaluate the diagnostic potential of the methods and to correlate them to allergen-specific...


    pub_type: 杂志文章


    authors: Michova A,Abugalia M,Ivanova Ts,Nikolov G,Taskov H,Petrunov B

    更新日期:2006-09-01 00:00:00

  • The management of anaphylaxis in childhood: position paper of the European academy of allergology and clinical immunology.

    abstract::Anaphylaxis is a growing paediatric clinical emergency that is difficult to diagnose because a consensus definition was lacking until recently. Many European countries have no specific guidelines for anaphylaxis. This position paper prepared by the EAACI Taskforce on Anaphylaxis in Children aims to provide practical g...


    pub_type: 杂志文章,实务指引


    authors: Muraro A,Roberts G,Clark A,Eigenmann PA,Halken S,Lack G,Moneret-Vautrin A,Niggemann B,Rancé F,EAACI Task Force on Anaphylaxis in Children.

    更新日期:2007-08-01 00:00:00

  • Nasal hyperreactivity in rhinitis: A diagnostic and therapeutic challenge.

    abstract::Although nasal hyperreactivity (NHR) is a common feature in patients suffering from allergic and nonallergic rhinitis, it is widely neglected during history taking, underdiagnosed in the majority of patients with rhinitis and rhinosinusitis, not considered as an outcome parameter in clinical trials on novel treatments...


    pub_type: 杂志文章,评审


    authors: Van Gerven L,Steelant B,Hellings PW

    更新日期:2018-09-01 00:00:00

  • Single-dose inhaled budesonide in subjects with chronic asthma.

    abstract::In 30 stable asthmatics, a comparison was made between the changes in pulmonary function (FEV1, FVC, PEF, MEF75, MEF50 and MEF25) hourly for 9 h after a single dose of inhaled budesonide 1,600 micrograms, and placebo. All subjects used inhaled steroids daily; this medication was, however, withheld 8 days prior to the ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Engel T,Dirksen A,Heinig JH,Nielsen NH,Weeke B,Johansson SA

    更新日期:1991-10-01 00:00:00

  • Full automation in allergy testing: measurement of specific IgE by the ENEA System.

    abstract:UNLABELLED:We evaluated the ENEA System, a fully automated instrument for the measurement of specific IgE antibodies. The instrument dispenses sera and reagents, incubates, washes, and reads and prints results automatically. The "core" of the instrument is the reactive unit called ACE (Allergy Chamber Enzymatic), which...


    pub_type: 杂志文章


    authors: Plebani M,Faggian D,Borghesan F

    更新日期:1995-03-01 00:00:00

  • Bee moth (Galleria mellonella) allergic reactions are caused by several thermolabile antigens.

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    pub_type: 杂志文章


    authors: Villalta D,Martelli P,Mistrello G,Roncarolo D,Zanoni D

    更新日期:2004-09-01 00:00:00