Staphylococcus aureus from atopic dermatitis skin accumulates in the lysosomes of keratinocytes with induction of IL-1α secretion via TLR9.


BACKGROUND:Staphylococcus aureus (S. aureus) is frequently detected in the skin of patients with atopic dermatitis (AD), and involved in the flare of AD. There are some evidence-specific strains of S. aureus affect the severity of AD. However, the mechanism of predominant colonization and the aggravation of dermatitis by certain strains of S. aureus in the patients with AD are still unknown. OBJECTIVE:To reveal the characteristics of S. aureus from patients with AD (S. aureus-AD), we analyzed the interaction of S. aureus-AD and keratinocytes in comparison with those of S. aureus laboratory strains (S. aureus-stand.). METHODS:We stimulated HaCaT cells, keratinocyte cell line, and human epidermal keratinocytes by heat-killed S. aureus strains, then evaluated immune response of keratinocytes by ELISA, immunofluorescence staining, and flow cytometry. RESULTS:Upon incubation with keratinocytes, three out of four strains of heat-killed S. aureus-AD were strongly agglutinated inside the cytoplasm. In the cells, they are located in lysosomes and promoted the secretion of interleukin-1α (IL-1α). These reactions were not observed by any of four strains of S. aureus-stand. and S. epidermidis and were abolished by the treatment of S. aureus with proteinase K. Moreover, the IL-1α secretion was diminished by the inhibition of Toll-like receptor 9 (TLR9). CONCLUSION:S. aureus-AD accumulates in lysosome of keratinocytes by means of bacterial cell wall proteins and induces IL-1α via TLR9.






Moriwaki M,Iwamoto K,Niitsu Y,Matsushima A,Yanase Y,Hisatsune J,Sugai M,Hide M




Has Abstract


2019-03-01 00:00:00














  • Nasal inhalation of budesonide from a spacer in children with perennial rhinitis and asthma.

    abstract::The standard treatment of allergic rhinitis and asthma consists of topical corticosteroids administered intranasally and inhaled through the mouth. Although this therapy is highly effective, and side-effects are few and mild, it may be possible further to improve the therapeutic index and patient compliance with the t...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Pedersen W,Hjuler I,Bisgaard H,Mygind N

    更新日期:1998-04-01 00:00:00

  • Oral immunotherapy of children with rhinoconjunctivitis due to birch pollen allergy. A double blind study.

    abstract::Thirty children with rhinoconjunctivitis due to birch pollinosis were treated in a double blind manner for 10 months with enteric-coated capsules containing either a birch pollen preparation (n = 14) with doses up to 1.4 X 10(6) biologic units per day or placebo (n = 16). Compared with the placebo group the actively t...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Möller C,Dreborg S,Lanner A,Björkstén B

    更新日期:1986-05-01 00:00:00

  • Excess subcutaneous tissue may preclude intramuscular delivery when using adrenaline autoinjectors in patients with anaphylaxis.

    abstract::Intramuscular adrenaline is the gold standard treatment for anaphylaxis. Intramuscular injection provides more rapid and higher plasma concentrations than subcutaneous routes. Given the increasing epidemic of obesity patients are at increased risk of subcutaneous delivery, we therefore assessed the depth of subcutaneo...


    pub_type: 杂志文章


    authors: Johnstone J,Hobbins S,Parekh D,O'Hickey S

    更新日期:2015-06-01 00:00:00

  • Sensitization to aeroallergens depends on environmental factors.

    abstract::Respiratory allergies and subclinical sensitization to aeroallergens were investigated in 129 rural and in 222 urban probands. The incidence of respiratory allergies was not significantly higher in the urban residents. Sensitization to aeroallergens was investigated with Phadezym RAST (house dust mite, Cladosporium, o...


    pub_type: 杂志文章


    authors: Popp W,Zwick H,Steyrer K,Rauscher H,Wanke T

    更新日期:1989-11-01 00:00:00

  • Eicosanoid mediator profiles in different phenotypes of nonsteroidal anti-inflammatory drug-induced urticaria.

    abstract:BACKGROUND:The role of arachidonic acid metabolites in NSAID-induced hypersensitivity has been studied in depth for NSAID-exacerbated respiratory disease (NERD) and NSAID-exacerbated cutaneous disease (NECD). However, no information is available for NSAID-induced urticarial/angioedema (NIUA), despite it being the most ...


    pub_type: 杂志文章


    authors: Doña I,Jurado-Escobar R,Perkins JR,Ayuso P,Plaza-Serón MC,Pérez-Sánchez N,Campo P,Bogas-Herrera G,Bartra J,Torres MJ,Sanak M,Cornejo-García JA

    更新日期:2019-06-01 00:00:00

  • Myeloid-derived suppressor cell-like fibrocytes are increased and associated with preserved lung function in chronic obstructive pulmonary disease.

    abstract:BACKGROUND:The role of fibrocytes in chronic obstructive pulmonary disease (COPD) is unknown. We sought to enumerate blood and tissue fibrocytes in COPD and determine the association of blood fibrocytes with clinical features of disease. METHODS:Utilizing flow cytometry to identify circulating, collagen type 1+ cells,...


    pub_type: 杂志文章


    authors: Wright AK,Newby C,Hartley RA,Mistry V,Gupta S,Berair R,Roach KM,Saunders R,Thornton T,Shelley M,Edwards K,Barker B,Brightling CE

    更新日期:2017-04-01 00:00:00

  • Siberian hamster: a new indoor source of allergic sensitization and respiratory disease.

    abstract:BACKGROUND:The identification of 'unknown' allergic sensitizations may determine the prognosis and treatment of patients with respiratory airway disease. Currently, the presence in homes of 'exotic' animals as pets is increasing. In this article the Siberian hamster or dwarf hamster (Phodopus sungorus) was identified a...


    pub_type: 杂志文章


    authors: Bertó JM,Peláez A,Fernández E,Lombardero M,Ferrer M

    更新日期:2002-02-01 00:00:00

  • Nature and function of gastrointestinal antigen-presenting cells.

    abstract::Antigen-presenting cells (APCs) of the human gut are heterogeneous, including both macrophages, a variety of dendritic cells and B cells. They are found both in gut-associated lymphoid tissue and in the mucosal lamina propria, especially beneath the surface epithelium. APCs have diverse phenotypes and therefore probab...


    pub_type: 杂志文章,评审


    authors: Brandtzaeg P

    更新日期:2001-01-01 00:00:00

  • Acute autoimmune response in a case of pyromellitic acid dianhydride-induced hemorrhagic alveolitis.

    abstract::A 17-year-old man was occupationally exposed to pyromellitic acid dianhydride dust during the production of epoxy resin in a chemical factory. He was clinically diagnosed as having acute hemorrhagic alveolitis associated with anemia. The serologic analysis revealed a high concentration of IgG antibodies against pyrome...


    pub_type: 杂志文章


    authors: Czuppon AB,Kaplan V,Speich R,Baur X

    更新日期:1994-05-01 00:00:00

  • Comparison of FEV1 and specific airway conductance in assessing airway response to occupational agents.

    abstract:BACKGROUND:There is theoretical evidence that specific airway conductance (SGaw) could be more reliable than forced expiratory volume in 1 s (FEV1) for assessing changes in airway calibre. We investigated the changes in FEV1 and SGaw when assessing bronchial responses to occupational agents. METHODS:SGaw and FEV1 were...


    pub_type: 杂志文章


    authors: Larbanois A,Delwiche JP,Jamart J,Vandenplas O

    更新日期:2003-12-01 00:00:00

  • Aspirin tolerance induced in aspirin-sensitive asthmatics.

    abstract::Twenty-nine patients with asthma and aspirin-sensitivity were studied in an attempt to induce tolerance to aspirin (ASA). Starting with the smallest ASA doses eliciting bronchial obstruction (threshold doses) we doubled the doses on subsequent days and finally achieved good tolerance of 600 mg ASA per day in 27 patien...


    pub_type: 杂志文章


    authors: Kowalski ML,Grzelewska-Rzymowska I,Rozniecki J,Szmidt M

    更新日期:1984-04-01 00:00:00

  • Regulatory T cells and type 2 innate lymphoid cell-dependent asthma.

    abstract::Group 2 innate lymphoid cells (ILC2s) are a recently identified group of cells with the potent capability to produce Th2-type cytokines such as interleukin (IL)-5 and IL-13. Several studies suggest that ILC2s play an important role in the development of allergic diseases and asthma. Activation of pulmonary ILC2s in mu...


    pub_type: 杂志文章,评审


    authors: Aron JL,Akbari O

    更新日期:2017-08-01 00:00:00

  • Monocytes enhance neutrophil-induced blister formation in an ex vivo model of bullous pemphigoid.

    abstract:BACKGROUND:Lesions of bullous pemphigoid (BP), an autoimmune subepidermal blistering disease characterized by the presence of tissue-bound and circulating autoantibodies to hemidesmosomal antigens, harbor a mixed inflammatory cellular infiltrate. In various models, neutrophils, eosinophils, mast cells, monocytes as wel...


    pub_type: 杂志文章


    authors: de Graauw E,Sitaru C,Horn MP,Borradori L,Yousefi S,Simon D,Simon HU

    更新日期:2018-05-01 00:00:00

  • Ragweed pollen collected along high-traffic roads shows a higher allergenicity than pollen sampled in vegetated areas.

    abstract:BACKGROUND:Pollutants may affect pollen allergenicity and thus the prevalence of allergies. Although a few studies are available in literature, the connection between pollution and the allergenic potential of pollen has yet to be clearly defined. The objective of this study was to evaluate the effect of traffic-related...


    pub_type: 杂志文章


    authors: Ghiani A,Aina R,Asero R,Bellotto E,Citterio S

    更新日期:2012-07-01 00:00:00

  • Impact of diet and the bacterial microbiome on the mucous barrier and immune disorders.

    abstract::The prevalence of chronic immune and metabolic disorders is increasing rapidly. In particular, inflammatory bowel diseases, obesity, diabetes, asthma and chronic obstructive pulmonary disease have become major healthcare and economic burdens worldwide. Recent advances in microbiome research have led to significant dis...


    pub_type: 杂志文章,评审


    authors: Alemao CA,Budden KF,Gomez HM,Rehman SF,Marshall JE,Shukla SD,Donovan C,Forster SC,Yang IA,Keely S,Mann ER,El Omar EM,Belz GT,Hansbro PM

    更新日期:2020-08-06 00:00:00

  • The effect of dry heat on mite, cat, and dog allergens.

    abstract:BACKGROUND:Various techniques have been tried in an attempt to reduce allergen levels in homes. This study investigated the effect of dry heat on mite, cat, and dog allergens. METHODS:Samples (50 mg) of Dermatophagoides pteronyssinus and D. farinae cultures, and of house dust rich in the major cat and dog allergens Fe...


    pub_type: 杂志文章


    authors: Cain G,Elderfield AJ,Green R,Smillie FI,Chapman MD,Custovic A,Woodcock A

    更新日期:1998-12-01 00:00:00

  • Leukocyte migration inhibition test in children with cow milk allergy.

    abstract::The usefulness of the leukocyte migration inhibition factor (LIF) test to detect cow milk (CM) hypersensitivity was studied in 40 children with suspected allergy to CM. Hypersensitivity was carefully investigated by oral milk challenges, which gave a final confirmation of cow milk hypersensitivity in 12 subjects, and ...


    pub_type: 杂志文章


    authors: Vanto T,Smogorzewska EM,Viander M,Kalimo K,Koivikko A

    更新日期:1987-11-01 00:00:00

  • Prevention of allergy by virus-like nanoparticles (VNP) delivering shielded versions of major allergens in a humanized murine allergy model.

    abstract:BACKGROUND:In high-risk populations, allergen-specific prophylaxis could protect from sensitization and subsequent development of allergic disease. However, such treatment might itself induce sensitization and allergies, thus requiring hypoallergenic vaccine formulations. We here characterized the preventive potential ...


    pub_type: 杂志文章


    authors: Kratzer B,Köhler C,Hofer S,Smole U,Trapin D,Iturri J,Pum D,Kienzl P,Elbe-Bürger A,Gattinger P,Mittermann I,Linhart B,Gadermaier G,Jahn-Schmid B,Neunkirchner A,Valenta R,Pickl WF

    更新日期:2019-02-01 00:00:00

  • Hypoxia inducible factor promotes murine allergic airway inflammation and is increased in asthma and rhinitis.

    abstract:BACKGROUND:New therapies are necessary to address inadequate asthma control in many patients. This study sets out to investigate whether hypoxia-inducible factor (HIF) is essential for development of allergic airway inflammation (AAI) and therefore a potential novel target for asthma treatment. METHODS:Mice conditiona...


    pub_type: 杂志文章


    authors: Huerta-Yepez S,Baay-Guzman GJ,Bebenek IG,Hernandez-Pando R,Vega MI,Chi L,Riedl M,Diaz-Sanchez D,Kleerup E,Tashkin DP,Gonzalez FJ,Bonavida B,Zeidler M,Hankinson O

    更新日期:2011-07-01 00:00:00

  • Detection of differentiation- and activation-linked cell surface antigens on cultured mast cell progenitors.

    abstract:BACKGROUND:Mast cells (MC) are multifunctional effector cells of the immune system. They derive from uncommitted CD34(+) hemopoietic progenitor cells (HPC). Depending on the stage of maturation and the environment, MC variably express differentiation- and activation-linked antigens. Little is known, however, about the ...


    pub_type: 杂志文章


    authors: Schernthaner GH,Hauswirth AW,Baghestanian M,Agis H,Ghannadan M,Worda C,Krauth MT,Printz D,Fritsch G,Sperr WR,Valent P

    更新日期:2005-10-01 00:00:00

  • Histopathological support of antagonism of allergen-induced mast cell mediator release in human skin by a beta-2-agonist.

    abstract::The site of antagonistic action on allergen-induced early skin reactions by the beta 2-agonist terbutaline was studied by light microscopy in 10 atopic subjects. Pretreatment with 1 microgram terbutaline intradermally 5 min prior to challenge with horse dander allergen produced an approximate inhibition of 85 and 55% ...


    pub_type: 杂志文章


    authors: Grönneberg R,Lagerholm B

    更新日期:1986-01-01 00:00:00

  • Rupatadine in allergic rhinitis and chronic urticaria.

    abstract::Histamine is the primary mediator involved the pathophysiology of allergic rhinitis and chronic urticaria, and this explains the prominent role that histamine H(1)-receptor antagonists have in the treatment of these disorders. However, histamine is clearly not the only mediator involved in the inflammatory cascade. Th...


    pub_type: 杂志文章,评审


    authors: Mullol J,Bousquet J,Bachert C,Canonica WG,Gimenez-Arnau A,Kowalski ML,Martí-Guadaño E,Maurer M,Picado C,Scadding G,Van Cauwenberge P

    更新日期:2008-04-01 00:00:00

  • Placebo-controlled challenges with perfume in patients with asthma-like symptoms.

    abstract::A group of nine patients with respiratory symptoms after nonspecific irritating stimuli, but without any IgE-mediated allergy or demonstrable bronchial obstruction, were referred to the asthma/allergy outpatient department for evaluation of suspected asthma. In order to find a provocation model and objectively assess ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Millqvist E,Löwhagen O

    更新日期:1996-06-01 00:00:00

  • Allergic disorders in African countries: linking immunology to accurate phenotype.

    abstract::Identification and characterization of risk and protective factors for allergy is important for developing strategies for prevention or treatment. The prevalence of allergy is clearly higher in affluent countries than in developing countries like, e.g. Africa. Especially in urban areas of developing countries, allergy...


    pub_type: 杂志文章,评审


    authors: van Ree R,Yazdanbakhsh M

    更新日期:2007-03-01 00:00:00

  • Risk factors for severe and critically ill COVID-19 patients: A review.

    abstract::The pandemic of coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused an unprecedented global social and economic impact, and high numbers of deaths. Many risk factors have been identified in the progression of COVID-19 into a severe and critical st...


    pub_type: 杂志文章,评审


    authors: Gao YD,Ding M,Dong X,Zhang JJ,Kursat Azkur A,Azkur D,Gan H,Sun YL,Fu W,Li W,Liang HL,Cao YY,Yan Q,Cao C,Gao HY,Brüggen MC,van de Veen W,Sokolowska M,Akdis M,Akdis CA

    更新日期:2020-11-13 00:00:00

  • Symptoms and immunologic markers induced by exposure to methyltetrahydrophthalic anhydride.

    abstract::A study was performed in 43 workers exposed to methyltetrahydrophthalic anhydride (MTHPA) used as a hardener in an epoxy resin system. Ten workers sensitized to MTHPA (group SS; presence of serum IgE antibodies against a conjugate of MTHPA and human serum albumin (HSA) detected by RAST) had significantly higher levels...


    pub_type: 杂志文章


    authors: Nielsen J,Welinder H,Bensryd I,Andersson P,Skerfving S

    更新日期:1994-04-01 00:00:00

  • Allergen-induced mediator release tests.

    abstract::The diagnosis of allergic reactions in clinical practice is based on both clinical history and the determination of specific immunoglobulin E (IgE), either in the serum or on skin mast cells. However, for various reasons, identification of the causative factors is not possible in all the cases. Moreover, not all aller...


    pub_type: 杂志文章,评审


    authors: Demoly P,Lebel B,Arnoux B

    更新日期:2003-07-01 00:00:00

  • Natural evolution of skin-test sensitivity in patients with IgE-mediated hypersensitivity to cephalosporins.

    abstract::There are studies demonstrating that skin-test sensitivity to penicillins can decrease over time and that allergic patients may lose sensitivity if the responsible compounds are avoided. With regard to subjects with IgE-mediated hypersensitivity to cephalosporins, however, such studies are lacking. We evaluated prospe...


    pub_type: 杂志文章


    authors: Romano A,Gaeta F,Valluzzi RL,Zaffiro A,Caruso C,Quaratino D

    更新日期:2014-06-01 00:00:00

  • Airway inflammation in asthma and perennial allergic rhinitis. Relationship with nonspecific bronchial responsiveness and maximal airway narrowing.

    abstract:BACKGROUND:Eosinophilic airway inflammation is the hallmark of asthma, but it has also been reported in other conditions such as allergic rhinitis. We have tested whether the analysis of cells and chemicals in sputum can distinguish between patients with mild allergic asthma, those with allergic rhinitis, and healthy c...


    pub_type: 杂志文章


    authors: Alvarez MJ,Olaguibel JM,García BE,Rodríquez A,Tabar AI,Urbiola E

    更新日期:2000-04-01 00:00:00

  • Lack of control of severe asthma is associated with co-existence of moderate-to-severe rhinitis.

    abstract:BACKGROUND:Retrospective studies provide evidence that rhinitis is associated with more severe asthma. The aim of this study was to evaluate prospectively whether rhinitis is a predictor of increased asthma severity. METHODS:Five hundred and fifty-seven patients with severe asthma were enrolled. During 1 year of follo...


    pub_type: 杂志文章


    authors: Ponte EV,Franco R,Nascimento HF,Souza-Machado A,Cunha S,Barreto ML,Naspitz C,Cruz AA

    更新日期:2008-05-01 00:00:00