Abstract：
:The nucleotide sequence of the portion of a Bacillus subtilis (strain PAP115) 3 kb Pst I fragment which contains an endo-beta-1, 4-glucanase gene has been determined. This gene encodes a protein of 499 amino acid residues (Mr = 55,234) with a typical B. subtilis signal peptide. Escherichia coli which has been transformed with this gene produces an extracellular endoglucanase with an amino-terminus corresponding to the thirtieth encoded amino acid residue. The gene is preceded by a cryptic reading frame with a rho-independent terminator structure, and itself has such a structure in the immediate 3'-flanking region. We have also identified, in the 5'-flanking region, nucleotide sequences which resemble promoter elements recognized by Bacillus RNA polymerase E sigma 43. Comparison of the encoded amino acid sequence to other known beta-glucanases reveals a small region of similarity to the encoded protein of the Clostridium thermocellum celB gene. These similar regions may contain substrate-binding and/or catalytic sites.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
MacKay RM,Lo A,Willick G,Zuker M,Baird S,Dove M,Moranelli F,Seligy Vdoi
10.1093/nar/14.22.9159subject
Has Abstractpub_date
1986-11-25 00:00:00pages
9159-70issue
22eissn
0305-1048issn
1362-4962journal_volume
14pub_type
杂志文章abstract：:Superhelical PM2 DNA can be photochemically modified by u.v. irradiation. The variation of S20,w with dose shows the following characteristics. There is a linear increase from 28 to 31s produced by a low dose of u.v. irradiation (4,000 ergs/mm2). A plateau in S20,w occurs between 4,000 and 10,000 ergs/mm2. The S20,w t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.5.1243
更新日期：1977-01-01 00:00:00
abstract：:Bacillus subtilis OhrR is a dimeric repressor that senses organic peroxides and regulates the expression of the OhrA peroxiredoxin. Derepression results from oxidation of an active site cysteine which ultimately results in formation of a mixed disulfide with a low molecular weight thiol, a cyclic sulfenamide, or overo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn1052
更新日期：2009-03-01 00:00:00
abstract：:Using a partially purified replication complex from tobacco chloroplasts, replication origins have been localized to minimal sequences of 82 (pKN8, positions 137 683-137 764) and 243 bp (pKN3, positions 130 513-130 755) for ori A and ori B respectively. Analysis of in vitro replication products by two-dimensional agar...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.18.3681
更新日期：1997-09-15 00:00:00
abstract：:Camptothecin (CPT), a plant alkaloid with antitumor activity, is a specific inhibitor of eukaryotic DNA topoisomerase I. We have previously isolated and characterized a CPT-resistant topoisomerase I isolated from a CPT-resistant human leukemia cell line, CPT-K5. cDNA clones of topoisomerase I were isolated from the CP...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.1.69
更新日期：1991-01-11 00:00:00
abstract：:Deproteinated 16S RNA was iodinated at pH 5.0 in an aqueous solution containing TlCl3 plus KI for 1-5 hours at 42 degrees C. Under these conditions 33 moles of iodine are incorporated per mole of RNA. As judged by sucrose gradient sedimentation, the iodinated RNA does not exhibit any large alteration in conformation a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.11.3001
更新日期：1976-11-01 00:00:00
abstract：:Pfam is a widely used database of protein families, currently containing more than 13,000 manually curated protein families as of release 26.0. Pfam is available via servers in the UK (http://pfam.sanger.ac.uk/), the USA (http://pfam.janelia.org/) and Sweden (http://pfam.sbc.su.se/). Here, we report on changes that ha...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1065
更新日期：2012-01-01 00:00:00
abstract：:Pax-5, a member of the paired domain family of transcription factors, is a key regulator of B lymphocyte-specific transcription and differentiation. A major target of Pax-5-mediated activation is the mb-1 gene, which encodes the essential transmembrane signaling protein Ig-alpha. Pax-5 recruits three members of the Et...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg785
更新日期：2003-10-01 00:00:00
abstract：:An improved algorithm was elaborated to analyse tRNA interaction with aminoacyl-tRNA synthetase based on analysis of tRNA sequences. The fundamental element defining the interaction between the tRNA and the synthetase is not a single nucleotide but a nucleotide combination named a tile which comprises of a given nucle...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.8.1941
更新日期：1993-04-25 00:00:00
abstract：:The UGA suppressor tRNA produced by Schizosaccharomyces pombe strain sup3-e was purified to homogeneity. It can be aminoacylated with a serine by a crude aminoacyl-tRNA synthetase preparation from S. pombe cells. By combining post-labeling fingerprinting and gel sequencing methods the nucleotide sequence of this tRNA ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.8.2683
更新日期：1979-06-25 00:00:00
abstract：:Highly toxic DNA double-strand breaks (DSBs) readily trigger the DNA damage response (DDR) in cells, which delays cell cycle progression to ensure proper DSB repair. In Saccharomyces cerevisiae, mitotic S phase (20-30 min) is lengthened upon DNA damage. During meiosis, Spo11-induced DSB onset and repair lasts up to 5 ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa587
更新日期：2020-09-04 00:00:00
abstract：:We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen metabolism. Expression of MLS1, which participates in the utilization of non-fermentable carbon sources, is sensitiv...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.21.5677
更新日期：1992-11-11 00:00:00
abstract：:The RecQ family of helicases is highly conserved both structurally and functionally from bacteria to humans. Defects in human RecQ helicases are associated with genetic diseases that are characterized by cancer predisposition and/or premature aging. RecQ proteins exhibit 3'-5' helicase activity and play critical roles...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz708
更新日期：2019-10-10 00:00:00
abstract：:A newly discovered group of spherical plant viruses contains a bipartite genome consisting of a single-strand linear RNA molecule (RNA 1, Mr 1.5 x 10(6) ), and a single-strand, covalently closed circular viroid-like RNA molecule (RNA 2, Mr approximately 125,000). The nucleotide sequences of the RNA 2 of two of these, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.12.3681
更新日期：1982-06-25 00:00:00
abstract：:Bile acid biosynthesis is subjected to feedback regulation whereby bile acids down-regulate their own synthesis. The major point of this regulation is at the level of cholesterol 7alpha-hydroxylase (7alpha-hydroxylase), which controls bile acid output from the classic pathway. This regulation is at the level of transc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.18.3587
更新日期：2000-09-15 00:00:00
abstract：:A positive cis-acting DNA element in the near 5'-upstream region of the CYP2B1/B2 genes in rat liver was found to play an important role in the transcription of these genes. An oligonucleotide covering -69 to -98 nt mimicked the gel mobility shift pattern given by the fragment -179 to +29 nt, which was earlier found a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.3.557
更新日期：1992-02-11 00:00:00
abstract：:Restriction mapping demonstrated the presence of several distinct proviral forms of mouse mammary tumor virus in the genome of GR mice. One of these proviruses (GR-MTV-2) was highly amplified in GR 3A cells, a cell line derived from a GR mammary tumor. By the criterion of restriction mapping, the amplified GR-MTV-2 pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.23.5715
更新日期：1980-12-11 00:00:00
abstract：:In 4M NaCl solutions (dC-dG)n (n = 3,4; approximately 9 mM) exist as a mixture o +/- B and Z forms. The low and high field components of two 31P NMR resonances originating from internal phosphodiester groups are assigned to the GpC and CpG linkages, respectively. Low temperatures stabilize the Z-forms, which completel...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.11.4625
更新日期：1984-06-11 00:00:00
abstract：:Chloroplast DNA, isolated from a synchronized culture of Chlamydomonas reinhardii, was digested with restriction endonucleases and examined in the electron microscope. Restriction fragments containing displacement loops (D-loop) were photographed and measured to determine the position of replicated sequences in relati...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.9.3843
更新日期：1984-05-11 00:00:00
abstract：:We have adapted a method for making libraries of mutations in any specific gene for use in the fission yeast Schizosaccharomyces pombe . This elegant and simple method consists of PCR amplification of the gene of interest, followed by co-transformation of fission yeast with the PCR fragment and a linearized plasmid ve...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.20.4783
更新日期：1998-10-15 00:00:00
abstract：:MODPROPEP is a web server for knowledge-based modeling of protein-peptide complexes, specifically peptides in complex with major histocompatibility complex (MHC) proteins and kinases. The available crystal structures of protein-peptide complexes in PDB are used as templates for modeling peptides of desired sequence in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm266
更新日期：2007-07-01 00:00:00
abstract：:Over the past 10 years, fluorescent end-labeling of DNA fragments has evolved into the preferred method of DNA detection for a wide variety of applications, including DNA sequencing and PCR fragment analysis. One of the advantages inherent in fluorescent detection methods is the ability to perform multi-color analyses...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.11.2797
更新日期：1998-06-01 00:00:00
abstract：:Selected groups of isolated 14C-labelled proteins from E. coli 30S ribosomal subunits were reconstituted with 32P-labelled 16S RNA, and the reconstituted complexes were partially digested with ribonuclease A. RNA fragments protected by the proteins were separated by gel electrophoresis and subjected to sequence analys...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.4.1233
更新日期：1988-02-25 00:00:00
abstract：:We have investigated the attachment of the DNA to the nuclear matrix during the division cycle of the plasmodial slime mold Physarum polycephalum. The DNA of plasmodia was pulse labelled at different times during the S phase and the label distribution was studied by graded DNase digestion of the matrix-DNA complexes p...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.4.1181
更新日期：1983-02-25 00:00:00
abstract：:Maintaining genome integrity is important for cells and damaged DNA triggers autoimmunity. Previous studies have reported that Three-prime repair exonuclease 1(TREX1), an endogenous DNA exonuclease, prevents immune activation by depleting damaged DNA, thus preventing the development of certain autoimmune diseases. Con...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx178
更新日期：2017-05-05 00:00:00
abstract：:We have sequenced the genome of Shigella flexneri serotype 2a, the most prevalent species and serotype that causes bacillary dysentery or shigellosis in man. The whole genome is composed of a 4 607 203 bp chromosome and a 221 618 bp virulence plasmid, designated pCP301. While the plasmid shows minor divergence from th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkf566
更新日期：2002-10-15 00:00:00
abstract：:A 30 nt RNA with a sequence designed to form an intramolecular triple helix was analyzed by one-and two-dimensional NMR spectroscopy and UV absorption measurements. NMR data show that the RNA contains seven pyrimidine-purine-pyrimidine base triples stabilized by Watson-Crick and Hoogsteen interactions. The temperature...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.14.2841
更新日期：1996-07-15 00:00:00
abstract：:In order to construct fish specific expression vectors for studies on gene regulation in vitro and in vivo a variety of heterologous enhancers and promoters from mammals and from viruses of higher vertebrate cells were tested for expression of the bacterial chloramphenicol acetyl transferase reporter gene in three tel...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.11.3299
更新日期：1990-06-11 00:00:00
abstract：:The C (pifC) protein of miniF represses transcription of its own gene by binding to the pif operator (pifO); it is also needed for replication initiated from the miniF primary origin (ori-1). We have determined the nucleotide sequence of the C gene. The gene has been inserted into an expression vector under Ptrp contr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.24.9699
更新日期：1986-12-22 00:00:00
abstract：:Contrary to the expectation, the Merrifield polystyrene resin, 2% cross-linked by divinylbenzene, is as efficient as the polyacrylmorpholide resin for the synthesis of polydeoxyribonucleotides using a phosphotriester method. On the Merrifield resin, the tetradecamer, dTpCpGpTpCpApApCpTpGpGpCpTpT, and the hexadecamer, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.22.5507
更新日期：1980-11-25 00:00:00
abstract：:To prevent progressive telomere shortening as a result of conventional DNA replication, new telomeric DNA must be added onto the chromosome end. The de novo DNA synthesis involves elongation of the G-rich strand of the telomere by telomerase. In human cells, the CST complex (CTC1-STN1-TEN1) also functions in telomere ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx125
更新日期：2017-05-05 00:00:00
