Abstract：
:We have investigated the attachment of the DNA to the nuclear matrix during the division cycle of the plasmodial slime mold Physarum polycephalum. The DNA of plasmodia was pulse labelled at different times during the S phase and the label distribution was studied by graded DNase digestion of the matrix-DNA complexes prepared from nuclei isolated by extraction with 2 M NaCl. Pulse labelled DNA was preferentially recovered from the matrix bound residual DNA at any time of the S phase. Label incorporated at the onset of the S phase remained preferentially associated with the matrix during the G2 phase and the subsequent S phase. The occurrence of the pulse label in the matrix associated DNA regions was transiently elevated at the onset of the subsequent S phase. Label incorporated at the end of the S phase was located at DNA regions which, in the G2 phase, were preferentially released from the matrix by DNase treatment. From the results and previously reported data on the distribution of attachment sites it can be concluded that origins of replicons or DNA sites very close to them are attached to the matrix during the entire nuclear cycle. The data further indicate that initiations of DNA replication occur at the same origins in successive S phases. Replicating DNA is bound to the matrix, in addition, by the replication fork or a region close to it. This binding is loosened after completion of the replication.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Aelen JM,Opstelten RJ,Wanka Fdoi
10.1093/nar/11.4.1181subject
Has Abstractpub_date
1983-02-25 00:00:00pages
1181-95issue
4eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract：:Extracellular and intracellular barriers typically prevent non-viral gene vectors from having an effective transfection efficiency. Formulation of a gene delivery vehicle that can overcome the barriers is a key step for successful tissue regeneration. We have developed a novel core-shelled DNA nanoparticle by invoking...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni171
更新日期：2005-11-03 00:00:00
abstract：:Human thymine-DNA glycosylase (TDG) is well known to excise thymine and uracil from G.T and G.U mismatches, respectively, and was therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine. In this study, we characterized ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg344
更新日期：2003-05-01 00:00:00
abstract：:Differential scanning microcalorimetry (DSC), UV absorption and circular dichroism (CD) have been used to study structure and stability of linear (lin), open circular (oc), supercoiled (cd) and relaxed circular duplex (rd) DNA and calf thymus (CT) DNA. Investigations were made in low salt buffer and in the presence of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.24.11737
更新日期：1988-12-23 00:00:00
abstract：:Much effort has long been devoted to unraveling the coordinated cellular response to genotoxic insults. In view of the difficulty of obtaining human biological samples of homogeneous origin, I have established a set of stable human clones where one DNA repair gene has been stably silenced by means of RNA interference....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm195
更新日期：2007-01-01 00:00:00
abstract：:Ribosomal protein L4 of Escherichia coli regulates expression of its own eleven gene S10 operon both by inhibiting translation and by stimulating premature termination of transcription. Both regulatory processes presumably involve L4 recognition of the S10 leader RNA. To help define L4's regulatory target, we have inv...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.10.2429
更新日期：1993-05-25 00:00:00
abstract：:Anti-microRNA (miRNA) oligonucleotides (AMOs) with 2'-O-Methyl (2'OMe) residues are commonly used to study miRNA function and can achieve high potency, with low cytotoxicity. Not withstanding this, we demonstrate the sequence-dependent capacity of 2'OMe AMOs to inhibit Toll-like receptor (TLR) 7 and 8 sensing of immun...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1343
更新日期：2015-01-01 00:00:00
abstract：:Gramene (http://www.gramene.org) is a knowledgebase for comparative functional analysis in major crops and model plant species. The current release, #54, includes over 1.7 million genes from 44 reference genomes, most of which were organized into 62,367 gene families through orthologous and paralogous gene classificat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1111
更新日期：2018-01-04 00:00:00
abstract：:Extragenic sequences in genomes, such as microRNA and CRISPR, are vital players in the cell. Repetitive extragenic palindromic sequences (REPs) are a class of extragenic sequences, which form nucleotide stem-loop structures. REPs are found in many bacterial species at a high copy number and are important in regulation...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks741
更新日期：2012-10-01 00:00:00
abstract：:Sequencing of the 3'-untranslated region of the CCT8 gene of the fungal pathogen Candida albicans revealed that the CCT8 coding region overlaps 13 bp with the coding region of the convergently orientated TRP1 gene. The same overlap was found in three strains with different genetic backgrounds. 3'-RACE was used to dete...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.22.5061
更新日期：1998-11-15 00:00:00
abstract：:A 170bp long BamHI-Sau3A DNA fragment from the actIII-actI intergenic region of the actinorhodin (Act) biosynthetic gene cluster of Streptomyces coelicolor A3(2) contains two promoters directing transcription in a divergent manner. One of them, the actIII promoter, is responsible for the transcription of the actIII ge...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.10.2623
更新日期：1991-05-25 00:00:00
abstract：:Resolvase enzymes that cleave DNA four-way (Holliday) junctions are required for poxvirus replication, but clinically useful inhibitors have not been developed. Here, we report an assay for resolvase cleavage activity based on fluorescence polarization (FP) for high-throughput screening and mechanistic studies. Initia...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks325
更新日期：2012-09-01 00:00:00
abstract：:During carcinogenesis, cells are exposed to increased replication stress due to replication fork arrest at sites of DNA lesions and difficult to replicate genomic regions. Efficient fork restart and DNA repair are important for cancer cell proliferation. We previously showed that the ADP-ribosyltransferase PARP10 inte...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky658
更新日期：2018-09-28 00:00:00
abstract：:Modification of cytosine plays an important role in epigenetic regulation of gene expression and genome stability. Cytosine is converted to 5-methylcytosine (5mC) by DNA methyltransferase; in turn, 5mC may be oxidized to 5-hydroxymethylcytosine (5hmC) by ten-eleven translocation enzyme. The structural flexibility of D...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1210
更新日期：2021-01-25 00:00:00
abstract：:Transcription factors (TFs) regulate gene expression by binding to short DNA sequence motifs, yet their binding specificities alone cannot explain how certain TFs drive a diversity of biological processes. In order to investigate the factors that control the functions of the pleiotropic TF STAT3, we studied its genome...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1300
更新日期：2013-02-01 00:00:00
abstract：:Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl772
更新日期：2006-01-01 00:00:00
abstract：:The three-dimensional Poisson-Boltzmann equation for the distribution of counterion charge density around double helical DNA has been solved by an iterative procedure. These computations have been performed for 0.01M monovalent salt solutions. A systematic study of the ten possible sequences found among adjacent nucle...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.3.1479
更新日期：1986-02-11 00:00:00
abstract：:Mollusca represents the second largest animal phylum but remains poorly explored from a genomic perspective. While the recent increase in genomic resources holds great promise for a deep understanding of molluscan biology and evolution, access and utilization of these resources still pose a challenge. Here, we present...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa918
更新日期：2021-01-08 00:00:00
abstract：:Vaccinia virus DNA topoisomerase catalyzes resolution of synthetic Holliday junctions in vitro. The mechanism entails concerted transesterifications at two recognition sites, 5'-CCCTT/, that are opposed within a partially mobile four-way junction. Efficient resolution occurs on a junction with a 10 bp segment of branc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.14.2658
更新日期：2000-07-15 00:00:00
abstract：:PubChem (http://pubchem.ncbi.nlm.nih.gov) is a public repository for biological activity data of small molecules and RNAi reagents. The mission of PubChem is to deliver free and easy access to all deposited data, and to provide intuitive data analysis tools. The PubChem BioAssay database currently contains 500,000 des...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1132
更新日期：2012-01-01 00:00:00
abstract：:DNA damage tolerance (DDT) is responsible for genomic stability and cell viability by bypassing the replication block. In Saccharomyces cerevisiae DDT employs two parallel branch pathways to bypass the DNA lesion, namely translesion DNA synthesis (TLS) and error-free lesion bypass, which are mediated by sequential mod...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw183
更新日期：2016-06-20 00:00:00
abstract：:One of the master regulators of placental cell fusion in mammals leading to multi-nucleated syncytiotrophoblasts is the transcription factor GCMa. Recently, we proved that the cAMP-driven protein kinase A signaling pathway is fundamental for up-regulation of GCMa transcript levels and protein stability. Here, we show ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn306
更新日期：2008-06-01 00:00:00
abstract：:The Raf-1 proto-oncogene product is a highly regulated serine/threonine kinase that functions in signal transduction downstream from growth factor receptors and upstream from nuclear proto-oncogene products. Using a transient cotransfection assay we have found that activated Raf-1 activates expression from the HIV-LTR...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.22.5229
更新日期：1993-11-11 00:00:00
abstract：:The plastid psbA promoter of tobacco was used with the aim to construct plastid specific marker genes. Upon transfer to the tobacco nuclear genome the plastid promoter fragment appeared to specify a messenger RNA. Placed 5' to the bar or nptII coding sequences the level of expression is sufficient to obtain a selectab...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.1.19
更新日期：1989-01-11 00:00:00
abstract：:TFIIB plays a pivotal role during assembly of the RNA polymerase II transcription preinitiation complex. TFIIB is composed of two domains that engage in an intramolecular interaction that can be disrupted by the VP16 activation domain. In this study, we describe a novel human TFIIB derivative harbouring two point muta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh504
更新日期：2004-03-22 00:00:00
abstract：:The nucleotide excision repair pathway removes ultraviolet (UV) photoproducts from the human genome in the form of short oligonucleotides ∼ 30 nt in length. Because there are limitations to many of the currently available methods for investigating UV photoproduct repair in vivo, we developed a convenient non-radioisot...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1179
更新日期：2014-02-01 00:00:00
abstract：:Ubiquitin mRNA was found to be an abundant transcript which was induced by heat shock (HS), and certain other stresses in mammalian cells. In Chinese hamster cells, the 2 major ubiquitin transcripts of 2.6 kb and 1.7 kb were induced coordinately, while a minor ubiquitin transcript of 0.8 kb was not induced; the respon...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.3.1215
更新日期：1989-02-11 00:00:00
abstract：:We have explored the functional relationships between spliceosome and Microprocessor complex activities in a novel class of microRNAs (miRNAs), named Splice site Overlapping (SO) miRNAs, whose pri-miRNA hairpins overlap splice sites. We focused on the evolutionarily conserved SO miR-34b, and we identified two indispen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt614
更新日期：2013-10-01 00:00:00
abstract：:A simple and efficient method is described to introduce structurally pre-determined mutations into recombinant genomes of filamentous phage M13. The method rests on gapped duplex DNA (gdDNA) molecules of the phage M13 genome as the key intermediate. In this gdDNA, the (+) and the (shorter) (-) strand carry different g...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.24.9441
更新日期：1984-12-21 00:00:00
abstract：:Proteins are highly dynamic molecules, whose function is intrinsically linked to their molecular motions. Despite the pivotal role of protein dynamics, their computational simulation cost has led to most structure-based approaches for assessing the impact of mutations on protein structure and function relying upon sta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky300
更新日期：2018-07-02 00:00:00
abstract：:We describe here the cloning, characterization and expression in E. coli of the gene coding for a DNA methylase from Spiroplasma sp. strain MQ1 (M.SssI). This enzyme methylates completely and exclusively CpG sequences. The Spiroplasma gene was transcribed in E. coli using its own promoter. Translation of the entire me...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.5.1145
更新日期：1990-03-11 00:00:00
