Abstract:
:The Raf-1 proto-oncogene product is a highly regulated serine/threonine kinase that functions in signal transduction downstream from growth factor receptors and upstream from nuclear proto-oncogene products. Using a transient cotransfection assay we have found that activated Raf-1 activates expression from the HIV-LTR. Analysis of a series of 5' deletion and point mutations revealed the NF-kappa B motifs as the Raf-responsive element in the HIV-LTR. Moreover, Raf-BXB activated expression from heterologous promoters driven by the HIV NF-kappa B binding sites. In addition to Raf, we show that v-Src, v-H-Ras and v-Mos activate HIV-LTR expression through the NF-kappa B binding sites and v-H-Ras-induced HIV-LTR expression is mediated by Raf-1. These findings may have implications for the involvement of the cellular homologues of these oncogenes in the switch from latent to productive infection by HIV in response to T-cell activation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Bruder JT,Heidecker G,Tan TH,Weske JC,Derse D,Rapp URdoi
10.1093/nar/21.22.5229subject
Has Abstractpub_date
1993-11-11 00:00:00pages
5229-34issue
22eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract::Unmethylated CpG rich islands are a feature of vertebrate DNA: they are associated with housekeeping and many tissue specific genes. CpG islands on the active X chromosome of mammals are also unmethylated. However, islands on the inactive X chromosome are heavily methylated. We have identified a CpG island in the 5' r...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.20.9527
更新日期:1988-10-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.14.2897
更新日期:1997-07-15 00:00:00
abstract::We describe the first polyA-containing processed pseudogene reported in the chicken. It includes a 0.52 kb open reading frame which could encode a 175 amino acid protein. The putative protein shows extensive homology to the ras oncogene superfamily, being most closely related to the yeast protein YP2. It is one of the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.5.2123
更新日期:1986-03-11 00:00:00
abstract::The Eukaryotic Promoter Database (EPD) is an annotated non-redundant collection of eukaryotic POL II promoters, experimentally defined by a transcription start site (TSS). Access to promoter sequences is provided by pointers to positions in the corresponding genomes. Promoter evidence comes from conventional TSS mappi...
journal_title:Nucleic acids research
pub_type: 历史文章,杂志文章
doi:10.1093/nar/gkj146
更新日期:2006-01-01 00:00:00
abstract::p73, the p53 homologue, exists as a transactivation-domain-proficient TAp73 or deficient deltaN(DN)p73 form. Expectedly, the oncogenic DNp73 that is capable of inactivating both TAp73 and p53 function, is over-expressed in cancers. However, the role of TAp73, which exhibits tumour-suppressive properties in gain or los...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn414
更新日期:2008-08-01 00:00:00
abstract::The replacement of thymidine with 5-bromo-2'-deoxyuridine (BrdU) is well-known to sensitize cells to ionizing radiation and photoirradiation. We reported here the sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex oligodeoxynucleotides harboring a BrdU or its closely rela...
journal_title:Nucleic acids research
pub_type: 杂志文章
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abstract::Reverse transcription in retroviruses and retrotransposons requires nucleic acid chaperones, which drive the rearrangement of nucleic acid conformation. The nucleic acid chaperone properties of the human immunodeficiency virus type-1 (HIV-1) nucleocapsid (NC) protein have been extensively studied, and nucleic acid agg...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr726
更新日期:2012-01-01 00:00:00
abstract::Molecular dynamics simulations were performed on the duplex DNA dodecamers d(CGCGAA TT CGCG): d(CGCGAATTCGCG) and d(GCACGAA TT AAG): d(CTTAATTCGTGC), where TT denotes a cis, syn cyclobutane thymine dimer. The constant temperature and pressure algorithm of the AMBER 4.1 molecular-modeling package was used with explicit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.7.1432
更新日期:1997-04-01 00:00:00
abstract::MS²PIP is a data-driven tool that accurately predicts peak intensities for a given peptide's fragmentation mass spectrum. Since the release of the MS²PIP web server in 2015, we have brought significant updates to both the tool and the web server. In addition to the original models for CID and HCD fragmentation, we hav...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1287
更新日期:2016-01-04 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkv516
更新日期:2015-07-13 00:00:00
abstract::Conditional gene targeting depends on tissue and time specificity of recombination events. Endogenous promoters are often used to drive various transgenic constructs. To avoid the problems associated with reconstituting a specific expression pattern in transgenic animals by this method, we tested the internal ribosome...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.6.1552
更新日期:1999-03-15 00:00:00
abstract::Sequencing by hybridization (SBH) approaches to DNA sequencing face two conflicting constraints. First, in order to ensure that the target DNA binds reliably, the oligonucleotide probes that are attached to the chip array must be >15 bp in length. Secondly, the total number of possible 15 bp oligonucleotides is too la...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh210
更新日期:2004-02-18 00:00:00
abstract::The nucleoside compositions of chloroplast and cytoplasmic tRNAPhe's from Euglena gracilis have been determined. The modified nucleoside compositions of these two tRNAs indicate that tRNAPheChl is more similar to procaryotic (E. coli) tRNAPhe than to either the Euglena cytoplasmic tRNAPhe or other eucaryotic cytoplasm...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.2.371
更新日期:1976-02-01 00:00:00
abstract::In DNA of the dinoflagellate Crypthecodinium cohnii, 38% of the thymine is replaced by the modified base 5-hydroxymethyluracil, and approximately 3% of the cytosine is replaced by 5-methylcytosine. Both of the modified bases are non-randomly distributed in the DNA. Determinations of 3' nearest neighbors show that HOMe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.20.4709
更新日期:1980-10-24 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.1.96
更新日期:1997-01-01 00:00:00
abstract::The topoisomerase I (TOP1) inhibitor irinotecan triggers cell death by trapping TOP1 on DNA, generating cytotoxic protein-linked DNA breaks (PDBs). Despite its wide application in a variety of solid tumors, the mechanisms of cancer cell resistance to irinotecan remains poorly understood. Here, we generated colorectal ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1026
更新日期:2017-02-17 00:00:00
abstract::Multicellular organismal development is controlled by a complex network of transcription factors, promoters and enhancers. Although reliable computational and experimental methods exist for enhancer detection, prediction of their target genes remains a major challenge. On the basis of available literature and ChIP-seq...
journal_title:Nucleic acids research
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doi:10.1093/nar/gkq1081
更新日期:2011-04-01 00:00:00
abstract::Therapeutic small interfering RNAs (siRNAs) are composed of chemically modified nucleotides, which enhance RNA stability and increase affinity in Watson-Crick base pairing. However, the precise fate of such modified nucleotides once the siRNA is degraded within the cell is unknown. Previously, we demonstrated that deo...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
abstract::Defined DNA substrates containing discrete abasic sites or paired abasic sites set 1, 3, 5 and 7 bases apart on opposite strands were constructed to examine the reactivity of S1, mung bean and P1 nucleases towards abasic sites. None of the enzymes acted on the substrate containing discrete abasic sites. Under conditio...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1995-10-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2010-07-01 00:00:00
abstract::The transcriptional potential of the hsp70 heat shock gene promoter is established prior to induction by stress. It has been shown previously that the TBP subunit of TFIID is associated with the TATA element and that RNA polymerase II is paused downstream from the transcription start site. In order to identify new int...
journal_title:Nucleic acids research
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更新日期:1995-08-25 00:00:00
abstract::We have used gel retardation analysis to show that human DNA topoisomerase IIbeta can bind a 40 bp linear duplex containing a single DNA topoisomerase IIbeta cleavage site. Furthermore, we demonstrate for the first time that human DNA topoisomerase IIbeta binds to four-way junction DNA. This supports previous suggesti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.4.984
更新日期:1999-02-15 00:00:00
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journal_title:Nucleic acids research
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更新日期:1987-04-24 00:00:00
abstract::We have carried out an extended set of standard and enhanced-sampling MD simulations (for a cumulative simulation time of 620 μs) with the aim to study folding landscapes of the rGGGUUAGGG and rGGGAGGG parallel G-hairpins (PH) with propeller loop. We identify folding and unfolding pathways of the PH, which is bridged ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky712
更新日期:2018-09-28 00:00:00
abstract::Acetyl-CoA Carboxylase catalyzes the first committed step in fatty acid synthesis. Escherichia coli acetyl-CoA carboxylase is composed of biotin carboxylase, carboxyltransferase and biotin carboxyl carrier protein functions. The accA and accD genes that code for the alpha- and beta-subunits, respectively, are not in a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1079
更新日期:2010-03-01 00:00:00
abstract::MobA protein, encoded by the broad host-range plasmid R1162, is required for conjugal mobilization of this plasmid. The protein is an essential part of the relaxosome, and is also necessary for the termination of strand transfer. In vitro, MobA is a nuclease specific for one of the two DNA strands of the origin of tra...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1993-09-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1986-03-25 00:00:00
abstract::Instability of complex mammalian genomic DNA inserts is commonplace in cosmid libraries constructed in conventional multicopy vectors. To develop a means to construct stable libraries, we have developed a low copy number cosmid vector based on the E. coli F factor replicon (Fosmid). We have tested relative stability o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.5.1083
更新日期:1992-03-11 00:00:00
abstract::Escherichia coli Exonuclease I (ExoI) digests single-stranded DNA (ssDNA) in the 3'-5' direction in a highly processive manner. The crystal structure of ExoI, determined previously in the absence of DNA, revealed a C-shaped molecule with three domains that form a central positively charged groove. The active site is a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt278
更新日期:2013-06-01 00:00:00