Abstract：
:Sequencing of the 3'-untranslated region of the CCT8 gene of the fungal pathogen Candida albicans revealed that the CCT8 coding region overlaps 13 bp with the coding region of the convergently orientated TRP1 gene. The same overlap was found in three strains with different genetic backgrounds. 3'-RACE was used to determine that the CCT8 and TRP1 transcripts extended significantly into the coding region of the adjacent gene, which also contained sequences encoding the poly(A) addition site. A strain retaining one wild-type CCT8/TRP1 locus on one chromosome and a deletion on the other homologous chromosome contained both CCT8 and TRP1 transcripts; this result indicates that both transcripts are synthesized from the same gene locus. The CCT8/TRP1 gene pair of C . albicans constitutes an extreme natural case of transcriptional overlap in a eukaryote. The results confirm that convergent overlapping transcription units are compatible with expression of the overlapping genes.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Gerads M,Ernst JFdoi
10.1093/nar/26.22.5061subject
Has Abstractpub_date
1998-11-15 00:00:00pages
5061-6issue
22eissn
0305-1048issn
1362-4962pii
gkb818journal_volume
26pub_type
杂志文章abstract：:The 3' splice site of the second intron (I2) of the human apolipoprotein-AII gene, (GT)16GGGCAG, is unique in that, although fully functional, a stretch of alternating guanine and thymine residues replaces the polypyrimidine tract usually associated with 3' splice junctions. The transient expression of successive 5' d...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.9.3787
更新日期：1987-05-11 00:00:00
abstract：:We have sequenced the external transcribed spacer (ETS) of a ribosomal transcription unit from Xenopus laevis, together with sections of the preceding non-transcribed spacer. Our analysis was carried out on the same cloned transcription unit as that from which the internal transcribed spacers (ITS) were previously seq...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.7.2387
更新日期：1982-04-10 00:00:00
abstract：:It is well established that gene regulation can be achieved through activator and repressor proteins that bind to DNA and switch particular genes on or off, and that complex metabolic networks determine the levels of transcription of a given gene at a given time. Using three complementary computational techniques to s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw815
更新日期：2016-11-02 00:00:00
abstract：:Proteins are highly dynamic molecules, whose function is intrinsically linked to their molecular motions. Despite the pivotal role of protein dynamics, their computational simulation cost has led to most structure-based approaches for assessing the impact of mutations on protein structure and function relying upon sta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky300
更新日期：2018-07-02 00:00:00
abstract：:The Génolevures online database (http://cbi.labri.fr/Genolevures/ and http://genolevures.org/) provides exploratory tools and curated data sets relative to nine complete and seven partial genome sequences determined and manually annotated by the Génolevures Consortium, to facilitate comparative genomic studies of Hemi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn859
更新日期：2009-01-01 00:00:00
abstract：:Recent evidence indicates a link between Parkinson's Disease (PD) and the expression of a-synuclein (SNCA) isoforms with different 3' untranslated regions (3'UTRs). Yet, the post-transcriptional mechanisms regulating SNCA expression are unknown. Using a large-scale in vitro /in silico screening we identified RNA-bindi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1048
更新日期：2017-12-15 00:00:00
abstract：:Applications of conditional gene expression, whether for therapeutic or basic research purposes, are increasingly requiring mammalian gene control systems that exhibit far tighter control properties. While numerous approaches have been used to improve the widely used Tet-regulatory system, many applications, particula...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn443
更新日期：2008-09-01 00:00:00
abstract：:Calf thymus DNA polymerase alpha (pol alpha) and bacteriophage T4 DNA polymerase (pol T4) were exploited as model enzymes to investigate the molecular mechanism of inhibitory action of N2-(p-n-butylphenyl)dGTP (BuPdGTP) and 2-(p-n-butyl-anilino)dATP (BuAdATP) on the BuPdNTP-susceptible alpha polymerase family. Kinetic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.7.1627
更新日期：1991-04-11 00:00:00
abstract：:We present evidence that the splice sites in mammalian pre-mRNAs are brought together via a three dimensional diffusion mechanism. We tested two mechanisms for splice site pairing: a lateral diffusion ('scanning') model and the currently favored three dimensional diffusion ('jumping') model. Two lines of evidence that...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.9.1638
更新日期：1996-05-01 00:00:00
abstract：:Aging research is a multi-disciplinary field encompassing knowledge from many areas of basic, applied and clinical research. Age-related processes occur on molecular, cellular, tissue, organ, system, organismal and even psychological levels, trigger the onset of multiple debilitating diseases and lead to a loss of fun...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1287
更新日期：2016-01-04 00:00:00
abstract：:We have carried out a survey of potential quadruplex structure sequences (PQSS), which occur in the immediate upstream region (500 bp) of human genes. By examining the number and distribution of these we have established that there is a clear link between them and the occurrence of the SP1-binding element 'GGGCGG', su...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn078
更新日期：2008-05-01 00:00:00
abstract：:ZFP57 is necessary for maintaining repressive epigenetic modifications at Imprinting control regions (ICRs). In mouse embryonic stem cells (ESCs), ZFP57 binds ICRs (ICRBS) and many other loci (non-ICRBS). To address the role of ZFP57 on all its target sites, we performed high-throughput and multi-locus analyses of inb...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw505
更新日期：2016-09-30 00:00:00
abstract：:Current approaches to design efficient antisense RNAs (asRNAs) rely primarily on a thermodynamic understanding of RNA-RNA interactions. However, these approaches depend on structure predictions and have limited accuracy, arguably due to overlooking important cellular environment factors. In this work, we develop a bio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx115
更新日期：2017-05-19 00:00:00
abstract：:The deoxyoctanucleotide 5'd (AAGGAGGT) which is complementary to the 3' terminus of 16S RNA has been used as a probe to measure the potential of this rRNA region to engage in intermolecular basepairing. The site specific binding of the octanucleotide is shown by labeling 16S RNA in situ at its 3' end with [32P]pCp and...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.6.1425
更新日期：1981-03-25 00:00:00
abstract：:Poly (ADP-ribose) polymerase 1 (PARP1) has emerged as an attractive target for cancer therapy due to its key role in DNA repair processes. Inhibition of PARP1 in BRCA-mutated cancers has been observed to be clinically beneficial. Recent genome-mapping experiments have identified a non-canonical G-quadruplex-forming se...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1179
更新日期：2019-02-20 00:00:00
abstract：:The detailed conformational features and dynamics of the naturally occurring deoxyribotrinucleoside diphosphates d-TpTpT and d-TpTpC have been investigated at 20 degrees C and 80 degrees C in aqueous solution by nuclear magnetic resonance spectroscopy. The observed NMR parameters indicate that the conformational prope...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.11.4399
更新日期：1978-11-01 00:00:00
abstract：:Major efforts are underway to elucidate the spatial distribution of ribosomal RNAs (rRNAs) in the ribosome. An especially informative approach is the identification of likely base-base tertiary interactions within the RNA by phylogenetic comparison. Herein evidence is presented for three heretofore unrecognized candid...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.6.2215
更新日期：1989-03-25 00:00:00
abstract：:In eukaryotes, DNA is packaged into a basic unit, the nucleosome which consists of 147 bp of DNA wrapped around a histone octamer composed of two copies each of the histones H2A, H2B, H3 and H4. Nucleosome structures are diverse not only by histone variants, histone modifications, histone composition but also through ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv276
更新日期：2015-04-30 00:00:00
abstract：:The template requirements for efficient adenovirus DNA replication were studied in vitro in a reconstituted system with cloned DNA fragments, containing the Ad2 origin region, as templates. Replication is enhanced by nuclear factor I, a cellular protein that binds specifically to the Ad2 origin. This stimulation is sh...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.13.4935
更新日期：1985-07-11 00:00:00
abstract：:A key complication in comparative genomics for reliable gene function prediction is the existence of duplicated genes. To study the effect of gene duplication on function prediction, we analyze orthologs between pairs of genomes where in one genome the orthologous gene has duplicated after the speciation of the two ge...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki913
更新日期：2005-10-27 00:00:00
abstract：:DNA and RNA guanine-quadruplexes (G4s) are stabilized by several cations, in particular by potassium and sodium ions. Generally, potassium stabilizes guanine-quartet assemblies to a larger extent than sodium; in this article we report about a higher-order G4 structure more stable in sodium than in potassium. Repeats o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw003
更新日期：2016-04-07 00:00:00
abstract：:This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A) x n poly(U) (n = 1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered struc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.18.4283
更新日期：1980-09-25 00:00:00
abstract：:The Saccharomyces Genome Database (SGD; http://www.yeastgenome.org) is an expertly curated database of literature-derived functional information for the model organism budding yeast, Saccharomyces cerevisiae. SGD constantly strives to synergize new types of experimental data and bioinformatics predictions with existin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1112
更新日期：2018-01-04 00:00:00
abstract：:2'-O-Aminooxymethyl ribonucleosides are prepared from their 3',5'-disilylated 2'-O-phthalimidooxymethyl derivatives by treatment with NH(4)F in MeOH. The reaction of these novel ribonucleosides with 1-pyrenecarboxaldehyde results in the efficient formation of stable and yet reversible ribonucleoside 2'-conjugates in y...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr896
更新日期：2012-03-01 00:00:00
abstract：:The Blocks Database contains multiple alignments of conserved regions in protein families which can be searched by e-mail (blocks@blocks.fhcrc.org) and World Wide Web (http://blocks.fhcrc.org/ ) servers to classify protein and nucleotide sequences. Recent enhancements to the servers include: (i) improved calculation o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.1.222
更新日期：1997-01-01 00:00:00
abstract：:Histone variants play an important role in shaping the mammalian epigenome and their aberrant expression is frequently observed in several types of cancer. However, the mechanisms that mediate their function and the composition of the variant-containing chromatin are still largely unknown. A proteomic interrogation of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku303
更新日期：2014-06-01 00:00:00
abstract：:Near-UV difference spectral analysis of the triplex formed from d(C-T)6 and d(A-G)6.d(C-T)6 in neutral and acidic solution shows that the third strand dC residues are protonated at pH 7.0, far above their intrinsic pKa. Additional support for ion-dipole interactions between the third strand dC residues and the G.C tar...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.14.2692
更新日期：1995-07-25 00:00:00
abstract：:MicroRNAs (miRNAs) can group together along the human genome to form stable secondary structures made of several hairpins hosting miRNAs in their stems. The few known examples of such structures are all involved in cancer development. A large scale computational analysis of human chromosomes crossing sequence analysis...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt112
更新日期：2013-04-01 00:00:00
abstract：:G-quadruplexes are dynamic structures folded in G-rich single-stranded DNA regions. These structures have been recognized as a potential nucleic acid based mechanism for regulating multiple cellular processes such as replication, transcription and genomic maintenance. So far, their transcriptional role in vivo during ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw011
更新日期：2016-05-19 00:00:00
abstract：:DynOmics (dynomics.pitt.edu) is a portal developed to leverage rapidly growing structural proteomics data by efficiently and accurately evaluating the dynamics of structurally resolved systems, from individual molecules to large complexes and assemblies, in the context of their physiological environment. At the core o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx385
更新日期：2017-07-03 00:00:00
