Abstract:
:This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A) x n poly(U) (n = 1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered structures, and it was extended to a wide range of salt concentrations at neutral pH. The dependence of stability of polymer helical order on hydrogen mass does not appear to be merely attributable to the stronger intrahelical deuterium bonding. Substituting Deuterium for Hydrogen implies a probably predominant modulation of the entrophy term of polymer-solvent interactions. Effects of deuteration on the width of the 2(poly(A) x poly (U)) leads to poly(A) x 2poly(U)+poly(A) interconversion reaction were also observed. They bear on the role of polymer-solvent interaction on pattern recognition leading to formation of ordered structures. They also bear on the role of the same interaction on the "breathing" of ordered structures of this type.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Cupane A,Vitrano E,San Biago PL,Madonia F,Palma MUdoi
10.1093/nar/8.18.4283subject
Has Abstractpub_date
1980-09-25 00:00:00pages
4283-303issue
18eissn
0305-1048issn
1362-4962journal_volume
8pub_type
杂志文章abstract::Previously, we have shown that all class-1 polypeptide release factors (RFs) share a common glycine-glycine-glutamine (GGQ) motif, which is critical for RF activity. Here, we subjected to site-directed mutagenesis two invariant amino acids, Gln185 and Arg189, situated in the GGQ minidomain of human eRF1, followed by d...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.19.3982
更新日期:2001-10-01 00:00:00
abstract::The generalized mismatch repair system of Streptococcus pneumoniae (the Hex system) can eliminate base pair mismatches arising in heteroduplex DNA during transformation or by DNA polymerase errors during replication. Mismatch repair is most likely initiated at nicks or gaps. The present work was started to examine the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.20.5525
更新日期:1991-10-25 00:00:00
abstract::Linear motifs are short, evolutionarily plastic components of regulatory proteins and provide low-affinity interaction interfaces. These compact modules play central roles in mediating every aspect of the regulatory functionality of the cell. They are particularly prominent in mediating cell signaling, controlling pro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1064
更新日期:2012-01-01 00:00:00
abstract::Bleomycin B(2)(BLM) in the presence of iron [Fe(II)] and O(2)catalyzes single-stranded (ss) and double-stranded (ds) cleavage of DNA. Electrospray ionization ion trap mass spectrometry was used to monitor these cleavage processes. Two duplex oligonucleotides containing an ethylene oxide tether between both strands wer...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.9.1978
更新日期:2000-05-01 00:00:00
abstract::Human minisatellite probes cross-hybridize to mouse DNA and detect multiple variable loci. The resulting DNA "fingerprints" vary substantially between inbred strains but relatively little within an inbred strain. By studying the segregation of variable DNA fragments in BXD recombinant inbred strains of mice, at least ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.7.2823
更新日期:1987-04-10 00:00:00
abstract::TopoisomeraseII (Topo II) is a major component of chromosomal scaffolds and essential for mitotic chromosome condensation, but the mechanism of this action remains unknown. Here, we used an in vitro chromatin reconstitution system in combination with atomic force and fluorescence microscopic analyses to determine how ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm116
更新日期:2007-01-01 00:00:00
abstract::A novel method to measure mRNA levels has been developed by combining the detection capabilities of RNase protection (RPA) with the quantification advantages of scintillation proximity assay (SPA) technology. Sample processing is reduced to the addition of a single reagent post RNase digestion. As a model system, the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.14.2947
更新日期:1997-07-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky953
更新日期:2019-01-10 00:00:00
abstract::Selected groups of isolated 14C-labelled proteins from E. coli 30S ribosomal subunits were reconstituted with 32P-labelled 16S RNA, and the reconstituted complexes were partially digested with ribonuclease A. RNA fragments protected by the proteins were separated by gel electrophoresis and subjected to sequence analys...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1988-02-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg215
更新日期:2003-02-15 00:00:00
abstract::An effective tool for the global analysis of both DNA methylation status and protein-chromatin interactions is a microarray constructed with sequences containing regulatory elements. One type of array suited for this purpose takes advantage of the strong association between CpG Islands (CGIs) and gene regulatory regio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki582
更新日期:2005-05-23 00:00:00
abstract::DNA triplet repeats, 5'-d(CTG)n and 5'-d(CAG)n, are present in genes which have been implicated in several neurodegenerative disorders. To investigate possible stable structures formed by these repeating sequences, we have examined d(CTG)n, d(CAG)n and d(CTG).d(CAG)n (n = 2 and 3) using NMR and UV optical spectroscopy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.21.4303
更新日期:1995-11-11 00:00:00
abstract::Phosphorylation of the cellular single-stranded DNA-binding protein, replication protein A (RPA), occurs during normal mitotic cell cycle progression and also in response to genotoxic stress. In budding yeast, these reactions require the ATM homolog Mec1, a central regulator of the DNA replication and DNA damage check...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.23.4808
更新日期:2001-12-01 00:00:00
abstract::We previously showed that histone H4 serine-1 phosphorylation (H4S1ph) is evolutionarily conserved during gametogenesis, and contributes to post-meiotic nuclear compaction and to full completion of sporulation in the yeast Saccharomyces cerevisiae. Previous studies showed that H4S1ph and another modification of the sa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq218
更新日期:2010-08-01 00:00:00
abstract::The deoxyribose hexanucleoside pentaphosphate (m5dC-dG)3 has been studied by 500 MHz 1H NMR in D2O (0.1 M NaCl) and in D2O/deuterated methanol mixtures. Two conformations, in slow equilibrium on the NMR time scale, were detected in methanolic solution. Two-dimensional nuclear Overhauser effect (NOE) experiments were u...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.2.1243
更新日期:1984-01-25 00:00:00
abstract::Pnkp is the end-healing and end-sealing component of an RNA repair system present in diverse bacteria from many phyla. Pnkp is composed of three catalytic modules: an N-terminal polynucleotide 5' kinase, a central 2',3' phosphatase and a C-terminal ligase. The phosphatase module is a Mn(2+)-dependent phosphodiesterase...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt221
更新日期:2013-06-01 00:00:00
abstract::The HIV RT and Protease Sequence Database is an online relational database that catalogs evolutionary and drug-related human immunodeficiency virus (HIV) reverse transcriptase (RT) and protease sequence variation (http://hivdb.stanford.edu). The database contains a compilation of nearly all published HIV RT and protea...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.1.346
更新日期:2000-01-01 00:00:00
abstract::We have previously investigated the role of the N-terminal region of ribosomal protein S4 to participate in 30S ribosome assembly and function (1-3). In this report we extend these studies to the two fragments produced by the chemical cleavage of protein S4 at the tryptophan residue 167. We find that the carboxyl term...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.17.6343
更新日期:1985-09-11 00:00:00
abstract::Silencing gene expression by siRNAs is rapidly becoming a powerful tool for the genetic analysis of mammalian cells. However, the rapid degradation of siRNA and the limited duration of its action call for an efficient delivery technology. Accordingly, we describe here that Atelocollagen complexed with siRNA is resista...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh093
更新日期:2004-07-22 00:00:00
abstract::Transfer RNAGlu4 was isolated from Drosophila melanogaster by affinity chromatography. The tRNA was iodinated "in vitro" with Na[25I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAGlu4 to the right arm of the second chro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.5.1465
更新日期:1978-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1978-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl582
更新日期:2006-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh913
更新日期:2004-10-27 00:00:00
abstract::The Metabolic Pathway Collection from EMP is an extraction of data from the larger Enzymes and Metabolic Pathways database (EMP). This extraction has been made publicly available in the hope that others will find it useful for a variety of purposes. The original release in October 1995 contained 1814 distinct pathways...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.1.37
更新日期:1997-01-01 00:00:00
abstract::Four pseudorevertants of a -10 region lacP mutation were isolated. Three of these mutations were found to activate nascent promoters. These mutations were: a -2 G/C----A/T change (-2A) promoting transcription at position +11, a +1 A/T----T/A change (+1T) promoting transcription initiation at position +13, and a +10 C/...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.10.3927
更新日期:1989-05-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.20.5311
更新日期:1992-10-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks164
更新日期:2012-06-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks630
更新日期:2012-09-01 00:00:00
abstract::Alu elements have repeatedly been found involved in gene rearrangements in humans. Although these elements have been suggested to stimulate gene rearrangements, sparse information is available for the possible mechanism(s) of these events. Here we present a compilation of Alu elements that have been involved in recomb...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.2.256
更新日期:1995-01-25 00:00:00
abstract::Emerging evidence suggests that recombinant adeno-associated viral (rAAV) vectors can be used for specific gene targeting in human somatic cells. We have developed an rAAV vector construction procedure employing fusion PCR and a single cloning step that considerably simplifies the knockout process. We demonstrate its ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh009
更新日期:2004-01-02 00:00:00