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A one- and two-dimensional NMR study of the B to Z transition of (m5dC-dG)3 in methanolic solution.

Abstract：

:The deoxyribose hexanucleoside pentaphosphate (m5dC-dG)3 has been studied by 500 MHz 1H NMR in D2O (0.1 M NaCl) and in D2O/deuterated methanol mixtures. Two conformations, in slow equilibrium on the NMR time scale, were detected in methanolic solution. Two-dimensional nuclear Overhauser effect (NOE) experiments were used to assign the base and many of the sugar resonances as well as to determine structural features for both conformations. The results were consistent with the an equilibrium in solution between B-DNA and Z-DNA. The majority of the molecules have a B-DNA structure in low-salt D2O and a Z-DNA structure at high methanol concentrations. A cross-strand NOE between methyl groups on adjacent cytosines is observed for Z-DNA but not B-DNA. The B-DNA conformation predominates at low methanol concentrations and is stabilized by increasing temperature, while the Z-DNA conformation predominates at high methanol concentrations and low temperatures. 31P NMR spectra gave results consistent with those obtained by 1H NMR. Comparison of the 31P spectra with those obtained on poly(dG-m5dC) allow assignment of the lower field resonances to GpC in the Z conformation.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Feigon J,Wang AH,van der Marel GA,Van Boom JH,Rich A

doi

10.1093/nar/12.2.1243

subject

Has Abstract

pub_date

1984-01-25 00:00:00

pages

1243-63

issue

2

eissn

0305-1048

issn

1362-4962

journal_volume

12

pub_type

杂志文章

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