The p16INK4a tumor suppressor controls p21WAF1 induction in response to ultraviolet light.


:p16INK4a and p21WAF1, two major cyclin-dependent kinase inhibitors, are the products of two tumor suppressor genes that play important roles in various cellular metabolic pathways. p21WAF1 is up-regulated in response to different DNA damaging agents. While the activation of p21WAF1 is p53-dependent following -rays, the effect of ultraviolet (UV) light on p21WAF1 protein level is still unclear. In the present report, we show that the level of the p21WAF1 protein augments in response to low UVC fluences in different mammalian cells. This up-regulation is mediated through the stabilization of p21WAF1 mRNA in a p16INK4a-dependent manner in both human and mouse cells. Furthermore, using p16-siRNA treated human skin fibroblast; we have shown that p16 controls the UV-dependent cytoplasmic accumulation of the mRNA binding HuR protein. In addition, HuR immunoprecipitations showed that UV-dependent binding of HuR to p21 mRNA is p16-related. This suggests that p16 induces p21 by enabling the relocalization of HuR from the nucleus to the cytoplasm. Accordingly, we have also shown that p16 is necessary for efficient UV-dependent p53 up-regulation, which also requires HuR. These results indicate that, in addition to its role in cell proliferation, p16INK4a is also an important regulator of the cellular response to UV damage.


Nucleic Acids Res


Nucleic acids research


Al-Mohanna MA,Al-Khalaf HH,Al-Yousef N,Aboussekhra A




Has Abstract


2007-01-01 00:00:00














  • Site-specific nicking at the avian retrovirus LTR circle junction by the viral pp32 DNA endonuclease.

    abstract::The avian retrovirus pp32 DNA endonuclease prefers to nick supercoiled DNA containing long terminal repeat (LTR) circle junction sequences at one or the other of two sites, each which mapped two nucleotides back from the circle junction. The sequence at the sites of nicking was (sequence: see text) where increases ind...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Grandgenett DP,Vora AC

    更新日期:1985-09-11 00:00:00

  • Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells.

    abstract::LINE-1 (L1) is a highly successful autonomous non-LTR retrotransposon and a major force shaping mammalian genomes. Although there are about 600 000 L1 copies covering 23% of the rat genome, full-length rat L1s (L1Rn) with intact open reading frames (ORFs) representing functional master copies for retrotransposition ha...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kirilyuk A,Tolstonog GV,Damert A,Held U,Hahn S,Löwer R,Buschmann C,Horn AV,Traub P,Schumann GG

    更新日期:2008-02-01 00:00:00

  • The incidence and distribution of CpG----TpG transitions in the coagulation factor IX gene. A fresh look at CpG mutational hotspots.

    abstract::The mutations of 76 haemophilia B patients representing the whole population registered with the Malmö haemophilia centre (42) and referrals from the UK, were characterised. RFLP haplotype analysis of the defective genes indicated that 51 single base pair substitutions were definitely of independent origin and 27 of t...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Green PM,Montandon AJ,Bentley DR,Ljung R,Nilsson IM,Giannelli F

    更新日期:1990-06-11 00:00:00

  • Structural and functional adaptation of Haloferax volcanii TFEα/β.

    abstract::The basal transcription factor TFE enhances transcription initiation by catalysing DNA strand-separation, a process that varies with temperature and ionic strength. Canonical TFE forms a heterodimeric complex whose integrity and function critically relies on a cubane iron-sulphur cluster residing in the TFEβ subunit. ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Blombach F,Ausiannikava D,Figueiredo AM,Soloviev Z,Prentice T,Zhang M,Zhou N,Thalassinos K,Allers T,Werner F

    更新日期:2018-03-16 00:00:00

  • Polymerization retardation isothermal amplification (PRIA): a strategy enables sensitively quantify genome-wide 5-methylcytosine oxides rapidly on handy instruments with nanoscale sample input.

    abstract::The current methods for quantifying genome-wide 5-methylcytosine (5mC) oxides are still scarce, mostly restricted with two limitations: assay sensitivity is seriously compromised with cost, assay time and sample input; epigenetic information is irreproducible during polymerase chain reaction (PCR) amplification withou...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chen D,Wang Y,Mo M,Zhang J,Zhang Y,Xu Y,Liu SY,Chen J,Ma Y,Zhang L,Dai Z,Cai C,Zou X

    更新日期:2019-11-04 00:00:00

  • Air2p is critical for the assembly and RNA-binding of the TRAMP complex and the KOW domain of Mtr4p is crucial for exosome activation.

    abstract::Trf4/5p-Air1/2p-Mtr4p polyadenylation complex (TRAMP) is an essential component of nuclear RNA surveillance in yeast. It recognizes a variety of nuclear transcripts produced by all three RNA polymerases, adds short poly(A) tails to aberrant or unstable RNAs and activates the exosome for their degradation. Despite the ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Holub P,Lalakova J,Cerna H,Pasulka J,Sarazova M,Hrazdilova K,Arce MS,Hobor F,Stefl R,Vanacova S

    更新日期:2012-07-01 00:00:00

  • Structural and functional characterization of a Dictyostelium gene encoding a DIF inducible, prestalk-enriched mRNA sequence.

    abstract::The pDd56 mRNA sequence is highly enriched in prestalk over prespore cells and is inducible by DIF, the putative Dictyostelium stalk-specific morphogen. We show that the pDd56 gene is composed of forty one copies of a twenty four amino acid, cysteine rich repeat. This is highly homologus to a repeat which we have prev...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ceccarelli A,McRobbie SJ,Jermyn KA,Duffy K,Early A,Williams JG

    更新日期:1987-09-25 00:00:00

  • Proton magnetic resonance studies of ultraviolet-irradiated apurinic acid.

    abstract::In apurinic acid, a single-stranded polydeoxyribonucleotide easily obtained upon depurination of DNA, the proton resonances arising from thymine and cytosine are readily observable in aqueous solution of 25 degrees C. Two methyl thymine resonances, centered at 1.88 ppm and separated by 0.045 ppm, are observed. We attr...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Rahn RO,Schleich T

    更新日期:1974-08-01 00:00:00

  • SNPWave: a flexible multiplexed SNP genotyping technology.

    abstract::Scalable multiplexed amplification technologies are needed for cost-effective large-scale genotyping of genetic markers such as single nucleotide polymorphisms (SNPs). We present SNPWave, a novel SNP genotyping technology to detect various subsets of sequences in a flexible fashion in a fixed detection format. SNPWave...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: van Eijk MJ,Broekhof JL,van der Poel HJ,Hogers RC,Schneiders H,Kamerbeek J,Verstege E,van Aart JW,Geerlings H,Buntjer JB,van Oeveren AJ,Vos P

    更新日期:2004-03-05 00:00:00

  • On the cooperative and noncooperative binding of ethidium to DNA.

    abstract::The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dC) X poly(dG-dC) has been studied by both phase partition and direct spectrophotometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E. coli DNA. On the other h...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Winkle SA,Rosenberg LS,Krugh TR

    更新日期:1982-12-20 00:00:00

  • Updates of the HbVar database of human hemoglobin variants and thalassemia mutations.

    abstract::HbVar ( is one of the oldest and most appreciated locus-specific databases launched in 2001 by a multi-center academic effort to provide timely information on the genomic alterations leading to hemoglobin variants and all types of thalassemia and hemoglobinopathies. Database records incl...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Giardine B,Borg J,Viennas E,Pavlidis C,Moradkhani K,Joly P,Bartsakoulia M,Riemer C,Miller W,Tzimas G,Wajcman H,Hardison RC,Patrinos GP

    更新日期:2014-01-01 00:00:00

  • Transcription regulation of the type II restriction-modification system AhdI.

    abstract::The Restriction-modification system AhdI contains two convergent transcription units, one with genes encoding methyltransferase subunits M and S and another with genes encoding the controller (C) protein and the restriction endonuclease (R). We show that AhdI transcription is controlled by two independent regulatory l...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Bogdanova E,Djordjevic M,Papapanagiotou I,Heyduk T,Kneale G,Severinov K

    更新日期:2008-03-01 00:00:00

  • Correction of the consequences of mitochondrial 3243A>G mutation in the MT-TL1 gene causing the MELAS syndrome by tRNA import into mitochondria.

    abstract::Mutations in human mitochondrial DNA are often associated with incurable human neuromuscular diseases. Among these mutations, an important number have been identified in tRNA genes, including 29 in the gene MT-TL1 coding for the tRNA(Leu(UUR)). The m.3243A>G mutation was described as the major cause of the MELAS syndr...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Karicheva OZ,Kolesnikova OA,Schirtz T,Vysokikh MY,Mager-Heckel AM,Lombès A,Boucheham A,Krasheninnikov IA,Martin RP,Entelis N,Tarassov I

    更新日期:2011-10-01 00:00:00

  • The University of Minnesota Biocatalysis/Biodegradation database: microorganisms, genomics and prediction.

    abstract::The University of Minnesota Biocatalysis/Biodegradation Database ( ) begins its fifth year having met its initial goals. It contains approximately 100 pathways for microbial catabolic metabolism of primarily xenobiotic organic compounds, including information on approximately 650 reacti...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ellis LB,Hershberger CD,Wackett LP

    更新日期:2000-01-01 00:00:00

  • The proofreading exonuclease subunit epsilon of Escherichia coli DNA polymerase III is tethered to the polymerase subunit alpha via a flexible linker.

    abstract::Escherichia coli DNA polymerase III holoenzyme is composed of 10 different subunits linked by noncovalent interactions. The polymerase activity resides in the alpha-subunit. The epsilon-subunit, which contains the proofreading exonuclease site within its N-terminal 185 residues, binds to alpha via a segment of 57 addi...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ozawa K,Jergic S,Park AY,Dixon NE,Otting G

    更新日期:2008-09-01 00:00:00

  • Genome-wide evidence for local DNA methylation spreading from small RNA-targeted sequences in Arabidopsis.

    abstract::Transposable elements (TEs) and their relics play major roles in genome evolution. However, mobilization of TEs is usually deleterious and strongly repressed. In plants and mammals, this repression is typically associated with DNA methylation, but the relationship between this epigenetic mark and TE sequences has not ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ahmed I,Sarazin A,Bowler C,Colot V,Quesneville H

    更新日期:2011-09-01 00:00:00

  • A conformational study of some adenosines by use of nuclear Overhauser effect.

    abstract::Conformations of 8-bromo-2'-[unk]-triisopropylbenzenesulfonyladenosine ([unk]) and its 3'-[unk]-isomer ([unk]) in solution have been determined by the use of intramolecular nuclear Overhauser effects in (1)H NMR spectroscopy. Compound [unk] has been proved to have a conformation in which the adenosine and benzene ring...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ueyama M,Tori K,Ikehara M,Kaneko M

    更新日期:1974-06-01 00:00:00

  • Combinatorial delivery of small interfering RNAs reduces RNAi efficacy by selective incorporation into RISC.

    abstract::Despite the great potential of RNAi, ectopic expression of shRNA or siRNAs holds the inherent risk of competition for critical RNAi components, thus altering the regulatory functions of some cellular microRNAs. In addition, specific siRNA sequences can potentially hinder incorporation of other siRNAs when used in a co...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Castanotto D,Sakurai K,Lingeman R,Li H,Shively L,Aagaard L,Soifer H,Gatignol A,Riggs A,Rossi JJ

    更新日期:2007-01-01 00:00:00

  • Peptidyl-tRNA hydrolase from Sulfolobus solfataricus.

    abstract::An enzyme capable of liberating functional tRNA(Lys) from Escherichia coli diacetyl-lysyl-tRNA(Lys) was purified from the archae Sulfolobus solfataricus. Contrasting with the specificity of peptidyl- tRNA hydrolase (PTH) from E.coli, the S.solfataricus enzyme readily accepts E.coli formyl-methionyl-tRNA(fMet) as a sub...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Fromant M,Ferri-Fioni ML,Plateau P,Blanquet S

    更新日期:2003-06-15 00:00:00

  • ProtoNet 4.0: a hierarchical classification of one million protein sequences.

    abstract::ProtoNet is an automatic hierarchical classification of the protein sequence space. In 2004, the ProtoNet (version 4.0) presents the analysis of over one million proteins merged from SwissProt and TrEMBL databases. In addition to rich visualization and analysis tools to navigate the clustering hierarchy, we incorporat...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kaplan N,Sasson O,Inbar U,Friedlich M,Fromer M,Fleischer H,Portugaly E,Linial N,Linial M

    更新日期:2005-01-01 00:00:00

  • Transcription of single-copy hybrid lacZ genes by T7 RNA polymerase in Escherichia coli: mRNA synthesis and degradation can be uncoupled from translation.

    abstract::In Escherichia coli transcription of individual genes generally requires concomitant translation, and thus the decay of mRNAs cannot be studied without the complication of translation. Here we have used T7 RNA polymerase to transcribe in vivo lacZ genes carrying ribosome binding sites of variable efficiency. We show t...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chevrier-Miller M,Jacques N,Raibaud O,Dreyfus M

    更新日期:1990-10-11 00:00:00

  • Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery.

    abstract::A reporter assay was developed to detect and quantify nonsense codon suppression by chemically aminoacylated tRNAs in mammalian cells. It is based on the cellular expression of the enhanced green fluorescent protein (EGFP) as a reporter for the site-specific amino acid incorporation in its sequence using an orthogonal...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ilegems E,Pick HM,Vogel H

    更新日期:2002-12-01 00:00:00

  • On the mechanism of the modular primer effect.

    abstract::Modular primers are strings of three contiguously annealed unligated oligonucleotides (modules) as short as 5- or 6-mers, selected from a presynthesized library. It was previously found that such strings can prime DNA sequencing reactions specifically, thus eliminating the need for the primer synthesis step in DNA seq...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Beskin AD,Zevin-Sonkin D,Sobolev IA,Ulanovsky LE

    更新日期:1995-08-11 00:00:00

  • Regulation of error-prone translesion synthesis by Spartan/C1orf124.

    abstract::Translesion synthesis (TLS) employs low fidelity polymerases to replicate past damaged DNA in a potentially error-prone process. Regulatory mechanisms that prevent TLS-associated mutagenesis are unknown; however, our recent studies suggest that the PCNA-binding protein Spartan plays a role in suppression of damage-ind...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kim MS,Machida Y,Vashisht AA,Wohlschlegel JA,Pang YP,Machida YJ

    更新日期:2013-02-01 00:00:00

  • Transient expression directed by homologous and heterologous promoter and enhancer sequences in fish cells.

    abstract::In order to construct fish specific expression vectors for studies on gene regulation in vitro and in vivo a variety of heterologous enhancers and promoters from mammals and from viruses of higher vertebrate cells were tested for expression of the bacterial chloramphenicol acetyl transferase reporter gene in three tel...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Friedenreich H,Schartl M

    更新日期:1990-06-11 00:00:00

  • Glycosyl conformational and inductive effects on the acid catalysed hydrolysis of purine nucleosides.

    abstract::The log kobs vs. pH profiles were determined in the intermediate acidity region for the glycosyl hydrolysis of guanosine and its 8-amino, 8-monomethylamino, 8-dimethylamino and 8-bromo derivatives. The decreased rate of the 8-amino and enhanced rate of the 8-bromo compound compared to guanosine support an A type mecha...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Jordan F,Niv H

    更新日期:1977-03-01 00:00:00

  • Assembly, nuclear import and function of U7 snRNPs studied by microinjection of synthetic U7 RNA into Xenopus oocytes.

    abstract::In Xenopus oocytes in vitro transcribed mouse U7 RNA is assembled into small nuclear ribonucleoproteins (snRNPs) that are functional in histone RNA 3' processing. If the special Sm binding site of U7 (AAUUUGUCUAG, U7 Sm WT) is converted into the canonical Sm sequence derived from the major snRNAs (AAUUUUUGGAG, U7 Sm O...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Stefanovic B,Hackl W,Lührmann R,Schümperli D

    更新日期:1995-08-25 00:00:00

  • Solution structure of duplex DNA containing an extrahelical abasic site analog determined by NMR spectroscopy and molecular dynamics.

    abstract::Translesional DNA synthesis past abasic sites proceeds with the preferential incorporation of dAMP opposite the lesion and, depending on the sequence context, one or two base deletions. High-resolution NMR spectroscopy and molecular dynamics simulations were used to determine the three-dimensional structure of a DNA h...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lin Z,Hung KN,Grollman AP,de los Santos C

    更新日期:1998-05-15 00:00:00

  • Stability of the primary organization of nucleosome core particles upon some conformational transitions.

    abstract::The sequential arrangement of histones along DNA in nucleosome core particles was determined between 0.5 and 600 mM salt and from 0 to 8 M urea. These concentrations of salt and urea up to 6 M had no significant effect on the linear order of histones along DNA but 8 M urea caused the rearrangement of histones. Conform...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Zayetz VW,Bavykin SG,Karpov VL,Mirzabekov AD

    更新日期:1981-03-11 00:00:00

  • SeqA structures behind Escherichia coli replication forks affect replication elongation and restart mechanisms.

    abstract::The SeqA protein binds hemi-methylated GATC sites and forms structures that sequester newly replicated origins and trail the replication forks. Cells that lack SeqA display signs of replication fork disintegration. The broken forks could arise because of over-initiation (the launching of too many forks) or lack of dyn...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Pedersen IB,Helgesen E,Flåtten I,Fossum-Raunehaug S,Skarstad K

    更新日期:2017-06-20 00:00:00