Abstract：
:We have analyzed the molecular mechanism that makes translation of the MS2 replicase cistron dependent on the translation of the upstream coat cistron. Deletion mapping on cloned cDNA of the phage shows that the ribosomal binding site of the replicase cistron is masked by a long distance basepairing to an internal coat cistron region. Removal of the internal coat cistron region leads to uncoupled replicase synthesis. Our results confirm the model as originally proposed by Min Jou et al. (1). Activation of the replicase start is sensitive to the frequency of upstream translation, but never reaches the level of uncoupled replicase synthesis.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Berkhout B,van Duin Jdoi
10.1093/nar/13.19.6955subject
Has Abstractpub_date
1985-10-11 00:00:00pages
6955-67issue
19eissn
0305-1048issn
1362-4962journal_volume
13pub_type
杂志文章abstract：:Structural domains are considered as the basic units of protein folding, evolution, function and design. Automatic decomposition of protein structures into structural domains, though after many years of investigation, remains a challenging and unsolved problem. Manual inspection still plays a key role in domain decomp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg189
更新日期：2003-02-01 00:00:00
abstract：:Two residues are invariant in all bZip basic regions: asparagine -18 and arginine -10 (we define the first leucine of the leucine zipper of GCN4 as +1). X-ray structures of two specific GCN4-DNA complexes (Ellenberger et al., Cell, 71, 1223-1237, 1992; König & Richmond, J. Mol. Biol., 233, 139-154, 1993) demonstrate t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.21.4395
更新日期：1994-10-25 00:00:00
abstract：:Alternative splicing has recently emerged as a major mechanism of regulation in the human genome, occurring in perhaps 40-60% of human genes. Thus, microarray studies of functional regulation could, in principle, be extended to detect not only the changes in the overall expression of a gene, but also changes in its sp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh173
更新日期：2004-12-14 00:00:00
abstract：:Despite much information as to the structure and function of the general transcription factors, little is known about the regulation of their expression. Transcription of the Saccharomyces cerevisiae SUA7 (TFIIB) gene results in the formation of two discrete transcripts. It was originally reported that the two transcr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.22.4435
更新日期：2000-11-15 00:00:00
abstract：:Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the resp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.18.3653
更新日期：1996-09-15 00:00:00
abstract：:TOP mRNAs are vertebrate transcripts which contain a 5'terminal oligopyrimidine tract (5'TOP), encode for ribosomal proteins and elongation factors 1alpha and 2, and are candidates for growth-dependent translational control mediated through their 5'TOP. In the present study we show that elongation factor 2 (EF2) mRNA ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.5.995
更新日期：1997-03-01 00:00:00
abstract：:Friedreich ataxia (FRDA) is caused by hyperexpansion of GAA*TTC repeats located in the first intron of the FXN gene, which inhibits transcription leading to the deficiency of frataxin. The FXN gene is an excellent target for therapeutic intervention since (i) 98% of patients carry the same type of mutation, (ii) the m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn604
更新日期：2008-11-01 00:00:00
abstract：:The histone variant H2A.Z has been implicated in many biological processes, such as gene regulation and genome stability. Here, we present the identification of H2A.Z.2.2 (Z.2.2), a novel alternatively spliced variant of histone H2A.Z and provide a comprehensive characterization of its expression and chromatin incorpo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks267
更新日期：2012-07-01 00:00:00
abstract：:Analysis of intergenic sequences for purposes such as the investigation of transcriptional signals or the identification of small RNA genes is frequently complicated by traditional biological database structures. Genome data is commonly treated as chromosome-length sequence records, detailed by gene calls demarcating ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg013
更新日期：2003-01-01 00:00:00
abstract：:RecBCD enzyme acts in the major pathway of homologous recombination of linear DNA in Escherichia coli. The enzyme unwinds DNA and is an ATP-dependent double-strand and single-strand exonuclease and a single-strand endonuclease; it acts at Chi recombination hotspots (5'-GCTGGTGG-3') to produce a recombinogenic single-s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.9.2125
更新日期：1998-05-01 00:00:00
abstract：:Mitochondrial-DNA diseases have no effective treatments. Allotopic expression-synthesis of a wild-type version of the mutated protein in the nuclear-cytosolic compartment and its importation into mitochondria-has been proposed as a gene-therapy approach. Allotopic expression has been successfully demonstrated in yeast...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq769
更新日期：2011-01-01 00:00:00
abstract：:Post-transcriptional modifications are characteristic features of tRNAs and have been shown in a number of cases to influence both their structural and functional properties, including structure stabilization, amino-acylation and codon recognition. We have developed an approach which allows the investigation of the po...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.3.756
更新日期：1999-02-01 00:00:00
abstract：:The replication origin (oriC) of the Escherichia coli chromosome has been cloned and the region essential for chromosomal replication has been delimited to 245 base pairs. In previous studies the ability of recombinants between oriC and ColE1-type vectors, to transform E. coli polA- strains was used to determine which...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.22.8007
更新日期：1983-11-25 00:00:00
abstract：:Rfam is a comprehensive collection of non-coding RNA (ncRNA) families, represented by multiple sequence alignments and profile stochastic context-free grammars. Rfam aims to facilitate the identification and classification of new members of known sequence families, and distributes annotation of ncRNAs in over 200 comp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki081
更新日期：2005-01-01 00:00:00
abstract：:We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Repl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.9.3083
更新日期：1977-09-01 00:00:00
abstract：:E2F transcription factors (E2F1-8) are known to coordinately regulate the expression of a plethora of target genes, including those coding for microRNAs (miRNAs), to control cell cycle progression. Recent work has described the atypical E2F factor E2F7 as a transcriptional repressor of cell cycle-related protein-codin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw146
更新日期：2016-07-08 00:00:00
abstract：:A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthrough of these RNAs i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks451
更新日期：2012-08-01 00:00:00
abstract：:These studies employed the synthetic linear DNA, poly dGdC, in the B and cobalt hexammine chloride (Co)-induced Z form to determine the effect of conformation on protein-DNA interactions. The rate of the reaction of the restriction endonucleases, Hha I and Cfo I, are reduced with Z DNA as compared to B DNA. The abilit...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.18.7237
更新日期：1986-09-25 00:00:00
abstract：:Therapeutic oligonucleotides are often modified using the phosphorothioate (PS) backbone modification which enhances stability from nuclease mediated degradation. However, substituting oxygen in the phosphodiester backbone with sulfur introduce chirality into the backbone such that a full PS 16-mer oligonucleotide is ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa031
更新日期：2020-02-28 00:00:00
abstract：:As more and more high-throughput data has been produced by next-generation sequencing, it is still a challenge to classify RNA transcripts into protein-coding or non-coding, especially for poorly annotated species. We upgraded our original coding potential calculator, CNCI (Coding-Non-Coding Index), to CNIT (Coding-No...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz400
更新日期：2019-07-02 00:00:00
abstract：:In vitro compartmentalization (IVC) was employed for the first time to select for novel bacteriophage lambda integrase variants displaying significantly enhanced recombination activity on a non-cognate target DNA sequence. These variants displayed up to 9-fold increased recombination activity over the parental enzyme,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1089
更新日期：2010-03-01 00:00:00
abstract：:Large-scale automatic annotation of protein sequences remains challenging in postgenomics era. E1DS is designed for annotating enzyme sequences based on a repository of 1D signatures. The employed sequence signatures are derived using a novel pattern mining approach that discovers long motifs consisted of several sequ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn324
更新日期：2008-07-01 00:00:00
abstract：:We describe conditions for producing uninterrupted expanded CTG repeats consisting of up to 2000 repeats using 29 DNA polymerase. Previously, generation of such repeats was hindered by CTG repeat instability in plasmid vectors maintained in Escherichia coli and poor in vitro ligation of CTG repeat concatemers due to s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn025
更新日期：2008-03-01 00:00:00
abstract：:The Zebrafish Information Network (ZFIN, http://zfin.org), the model organism database for zebrafish, provides the central location for curated zebrafish genetic, genomic and developmental data. Extensive data integration of mutant phenotypes, genes, expression patterns, sequences, genetic markers, morpholinos, map po...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm956
更新日期：2008-01-01 00:00:00
abstract：:Small inverted repeats (small palindromes) on plasmids have been shown to mediate a recombinational rearrangement event in Escherichia coli leading to the formation of inverted dimers (giant palindromes). This recombinational rearrangement event is efficient and independent of RecA and RecBCD. In this report, we propo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.15.3009
更新日期：1997-08-01 00:00:00
abstract：:Hepatitis B virus (HBV) replication is initiated by HBV RT binding to the highly conserved encapsidation signal, epsilon, at the 5' end of the RNA pregenome. Epsilon contains an apical stem-loop, whose residues are either totally conserved or show rare non-disruptive mutations. Here we present the structure of the api...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl582
更新日期：2006-01-01 00:00:00
abstract：:The use of the ammonia deprotection step to introduce (15)N labels at specific exocyclic amino positions of adenine, cytosine, guanine or 2-aminopurine of oligodeoxynucleotides is described. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.15.2982
更新日期：1994-08-11 00:00:00
abstract：:With the recent increase in the number of mammalian genomes being sequenced, large-scale genome scans for human-specific positive selection are now possible. Selection can be inferred through phylogenetic analysis by comparing the rates of silent and replacement substitution between related species. Maximum-likelihood...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm764
更新日期：2008-01-01 00:00:00
abstract：:The selective and potent inhibition of mitochondrial translation in Saccharomyces cerevisiae by pentamidine suggests a novel antimicrobial action for this drug. Electrophoresis mobility shift assay, T1 ribonuclease footprinting, hydroxyl radical footprinting and isothermal titration calorimetry collectively demonstrat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1180
更新日期：2008-03-01 00:00:00
abstract：:A non-redundant database of nuclear, protein-encoding, genomic DNA sequences highlighting nuclear pre-mRNA introns was constructed using information contained in the SWISS-PROT and GenBank sequence databases. This Intron DataBase (IDB) contains information about (i) introns (including nucleotide sequence, location, ph...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.1.181
更新日期：2000-01-01 00:00:00
