A stimulatory RNA associated with RecBCD enzyme.

abstract：:Cadmium (Cd2+) is a known carcinogen that inactivates the DNA mismatch repair (MMR) pathway. In this study, we have tested the effect of Cd2+ exposure on the enzymatic activity of the mismatch binding complex MSH2-MSH6. Our results indicate that Cd2+ is highly inhibitory to the ATP binding and hydrolysis activities of...

journal_title：Nucleic acids research

authors： Banerjee S,Flores-Rozas H

更新日期：2005-03-03 00:00:00

abstract：:Here we describe the application of a novel combinatorial method, restriction endonuclease protection selection and amplification (REPSA), to identification of a consensus DNA binding site for the TATA binding subunit (hTBP) of the human general transcription factor TFIID. Unlike most combinatorial methods, REPSA is b...

journal_title：Nucleic acids research

authors： Hardenbol P,Wang JC,Van Dyke MW

更新日期：1997-08-15 00:00:00

abstract：:The complete nucleotide sequence of the Escherichia coli recB gene which encodes a subunit of the ATP-dependent DNase, Exonuclease V, has been determined. The proposed coding region for the RecB protein is 3543 nucleotides long and would encode a polypeptide of 1180 amino acids with a calculated molecular weight of 13...

journal_title：Nucleic acids research

authors： Finch PW,Storey A,Chapman KE,Brown K,Hickson ID,Emmerson PT

更新日期：1986-11-11 00:00:00

abstract：:Conformations of 8-bromo-2'-[unk]-triisopropylbenzenesulfonyladenosine ([unk]) and its 3'-[unk]-isomer ([unk]) in solution have been determined by the use of intramolecular nuclear Overhauser effects in (1)H NMR spectroscopy. Compound [unk] has been proved to have a conformation in which the adenosine and benzene ring...

journal_title：Nucleic acids research

authors： Ueyama M,Tori K,Ikehara M,Kaneko M

更新日期：1974-06-01 00:00:00

abstract：:Adenosine deaminases that act on RNA (ADARs) convert adenosine to inosine within double-stranded regions of RNA, resulting in increased transcriptomic diversity, as well as protection of cellular double-stranded RNA (dsRNA) from silencing and improper immune activation. The presence of dsRNA-binding domains (dsRBDs) i...

journal_title：Nucleic acids research

authors： Rajendren S,Manning AC,Al-Awadi H,Yamada K,Takagi Y,Hundley HA

更新日期：2018-10-12 00:00:00

abstract：:Antimicrobial peptides (AMPs) are gaining importance as anti-infective agents. Here we describe the updated Collection of Antimicrobial Peptide (CAMP) database, available online at http://www.camp.bicnirrh.res.in/. The 3D structures of peptides are known to influence antimicrobial activity. Although there exists datab...

journal_title：Nucleic acids research

authors： Waghu FH,Gopi L,Barai RS,Ramteke P,Nizami B,Idicula-Thomas S

更新日期：2014-01-01 00:00:00

abstract：:Ribonuclease III of Escherichia coli is prominently involved in the endoribonucleolytic processing of cell and viral-encoded RNAs. Towards the goal of defining the RNA sequence and structural elements that establish specific catalytic cleavage of RNase III processing signals, this report demonstrates that a 60 nucleot...

journal_title：Nucleic acids research

authors： Chelladurai BS,Li H,Nicholson AW

更新日期：1991-04-25 00:00:00

abstract：:Three clones of non-repetitive sequences and six clones containing repetitive sequences were obtained from micronuclear DNA of Tetrahymena thermophila. All the non-repetitive and three repetitive sequences had the same organization in micro- and macronuclear DNAs as revealed by blot hybridization. On the other hand, t...

journal_title：Nucleic acids research

authors： Iwamura Y,Sakai M,Muramatsu M

更新日期：1982-07-24 00:00:00

abstract：:The tumor suppressor gene, p53, is rarely mutated in neuroblastomas (NB) at the time of diagnosis, but its dysfunction could result from a nonfunctional conformation or cytoplasmic sequestration of the wild-type p53 protein. However, p53 mutation, when it occurs, is found in NB tumors with drug resistance acquired ove...

journal_title：Nucleic acids research

authors： Goldschneider D,Horvilleur E,Plassa LF,Guillaud-Bataille M,Million K,Wittmer-Dupret E,Danglot G,de Thé H,Bénard J,May E,Douc-Rasy S

更新日期：2006-01-01 00:00:00

abstract：:The binary Cre-lox conditional knockout system requires an essential part of the target gene to be flanked by loxP sites, enabling excision in vivo upon Cre expression. LoxP sites are introduced by homologous recombination, together with a selectable marker. However, this marker can disturb gene expression and should ...

journal_title：Nucleic acids research

authors： Leneuve P,Colnot S,Hamard G,Francis F,Niwa-Kawakita M,Giovannini M,Holzenberger M

更新日期：2003-03-01 00:00:00

abstract：:The polymerase encoded by human hepatitis B virus, which has reverse transcriptase and RNase H activity, binds to its pregenomic RNA template in a two-step process involving a terminal redundancy. Both first strand and second strand DNA synthesis involve primer translocation and second strand synthesis involves a temp...

journal_title：Nucleic acids research

authors： Kidd AH,Kidd-Ljunggren K

更新日期：1996-09-01 00:00:00

abstract：:DNA polymerase beta (polbeta), a member of the X family of DNA polymerases, is the major polymerase in the base excision repair pathway. Using in vitro selection, we obtained RNA aptamers for polbeta from a variable pool of 8 x 10(12) individual RNA sequences containing 30 random nucleotides. A total of 60 individual ...

journal_title：Nucleic acids research

authors： Gening LV,Klincheva SA,Reshetnjak A,Grollman AP,Miller H

更新日期：2006-05-17 00:00:00

abstract：:RNA silencing is an evolutionarily conserved mechanism triggered by double-stranded RNA that is processed into 21- to 24-nt small interfering (si)RNA or micro (mi)RNA by RNaseIII-like enzymes called Dicers. Gene regulations by RNA silencing have fundamental implications in a large number of biological processes that i...

journal_title：Nucleic acids research

authors： Uddin MN,Dunoyer P,Schott G,Akhter S,Shi C,Lucas WJ,Voinnet O,Kim JY

更新日期：2014-07-01 00:00:00

abstract：:The HUGO Gene Nomenclature Committee (HGNC) based at EMBL's European Bioinformatics Institute (EMBL-EBI) assigns unique symbols and names to human genes. There are over 42,000 approved gene symbols in our current database of which over 19 000 are for protein-coding genes. While we still update placeholder and problema...

journal_title：Nucleic acids research

authors： Tweedie S,Braschi B,Gray K,Jones TEM,Seal RL,Yates B,Bruford EA

更新日期：2021-01-08 00:00:00

abstract：:Hammerhead ribozymes were transcribed from a dsDNA template containing four random nucleotides between stems II and III, which replace the naturally occurring GAA nucleotides. In vitro selection was used to select hammerhead ribozymes capable of in cis cleavage using denaturing polyacrylamide gels for the isolation of...

journal_title：Nucleic acids research

authors： Thomson JB,Sigurdsson ST,Zeuch A,Eckstein F

更新日期：1996-11-15 00:00:00

abstract：:Alternative splicing and polyadenylation were observed pervasively in eukaryotic messenger RNAs. These alternative isoforms could either be consequences of physiological regulation or stochastic noise of RNA processing. To quantify the extent of stochastic noise in splicing and polyadenylation, we analyzed the alterna...

journal_title：Nucleic acids research

authors： Hon CC,Weber C,Sismeiro O,Proux C,Koutero M,Deloger M,Das S,Agrahari M,Dillies MA,Jagla B,Coppee JY,Bhattacharya A,Guillen N

更新日期：2013-02-01 00:00:00

abstract：:Remodelling protein nucleic acid interfaces is an important biological task, which is often carried out by nucleic acid stimulated ATPases of the Swi2/Snf2 superfamily. Here we study the mechano-chemical cycle of such an ATPase, namely the catalytic domain of the Sulfolobus solfataricus Rad54 homologue (SsoRad54cd), b...

journal_title：Nucleic acids research

authors： Lewis R,Dürr H,Hopfner KP,Michaelis J

更新日期：2008-04-01 00:00:00

abstract：:We present the PATHOgenic YEAst Search for Transcriptional Regulators And Consensus Tracking (PathoYeastract - http://pathoyeastract.org) database, a tool for the analysis and prediction of transcription regulatory associations at the gene and genomic levels in the pathogenic yeasts Candida albicans and C. glabrata Up...

journal_title：Nucleic acids research

authors： Monteiro PT,Pais P,Costa C,Manna S,Sá-Correia I,Teixeira MC

更新日期：2017-01-04 00:00:00

abstract：:The U2 snRNP complex contains two specific proteins, U2B" and U2A'. We have analysed the interaction of U2A' with U2B" and with U2 RNA. U2A' can form an weak but detectable RNA-protein complex with U2 RNA and a stable protein complex with U2B". This protein-protein complex binds efficiently and specifically to U2 RNA....

journal_title：Nucleic acids research

authors： Boelens W,Scherly D,Beijer RP,Jansen EJ,Dathan NA,Mattaj IW,van Venrooij WJ

更新日期：1991-02-11 00:00:00

abstract：:We describe a dedicated RNA motif search program and web server to identify RNA riboswitches. The Riboswitch finder analyses a given sequence using the web interface, checks specific sequence elements and secondary structure, calculates and displays the energy folding of the RNA structure and runs a number of tests in...

journal_title：Nucleic acids research

authors： Bengert P,Dandekar T

更新日期：2004-07-01 00:00:00

abstract：:The MPI Bioinformatics Toolkit (http://toolkit.tuebingen.mpg.de) is an open, interactive web service for comprehensive and collaborative protein bioinformatic analysis. It offers a wide array of interconnected, state-of-the-art bioinformatics tools to experts and non-experts alike, developed both externally (e.g. BLAS...

journal_title：Nucleic acids research

authors： Alva V,Nam SZ,Söding J,Lupas AN

更新日期：2016-07-08 00:00:00

abstract：:We have studied the coil-to-helix transition of the DNA oligomer d(C4A4T4C4), using circular dichroism measurements to monitor the formation of A.T base pairs within the central self-complementary A4T4 region and the formation of protonated C.C+ base pairs at the ends of the oligomer. We found that both A.T and C.C+ b...

journal_title：Nucleic acids research

authors： Gray DM,Cui T,Ratliff RL

更新日期：1984-10-11 00:00:00

abstract：:Next generation sequencing is widely used to link genetic variants to diseases, and it has massively accelerated the diagnosis and characterization of rare genetic diseases. After initial bioinformatic data processing, the interactive analysis of genome, exome, and panel sequencing data typically starts from lists of ...

journal_title：Nucleic acids research

authors： Müller H,Jimenez-Heredia R,Krolo A,Hirschmugl T,Dmytrus J,Boztug K,Bock C

更新日期：2017-07-03 00:00:00

abstract：:Herein, we describe a case study into the population dynamics of in vitro selection, using RNA-cleaving DNAzymes as a model system. We sought to understand how the composition of the population can change over time in response to different levels of selection pressure, and how well these changes are correlated with se...

journal_title：Nucleic acids research

authors： Schlosser K,Lam JC,Li Y

更新日期：2009-06-01 00:00:00

abstract：:We have developed a new COFACTOR webserver for automated structure-based protein function annotation. Starting from a structural model, given by either experimental determination or computational modeling, COFACTOR first identifies template proteins of similar folds and functional sites by threading the target structu...

journal_title：Nucleic acids research

authors： Roy A,Yang J,Zhang Y

更新日期：2012-07-01 00:00:00

abstract：:Nucleic acids possess the unique property of being enzymatically amplifiable, and have therefore been a popular choice for the combinatorial selection of functional sequences, such as aptamers or ribozymes. However, amplification typically requires known sequence segments that serve as primer binding sites, which can ...

journal_title：Nucleic acids research

authors： Wiesmayr A,Fournier P,Jäschke A

更新日期：2012-05-01 00:00:00

abstract：:Neuroserpin is an axonally secreted serine protease inhibitor expressed in the nervous system that protects neurons from ischemia-induced apoptosis. Mutant neuroserpin forms have been found polymerized in inclusion bodies in a familial autosomal encephalopathy causing dementia, or associated with epilepsy. Regulation ...

journal_title：Nucleic acids research

authors： Cuadrado A,Navarro-Yubero C,Furneaux H,Kinter J,Sonderegger P,Muñoz A

更新日期：2002-05-15 00:00:00

abstract：:The bZip proteins GCN4 and C/EBP differ in their DNA binding specificities: GCN4 binds well to the pseudopalindromic AP1 site 5'-A4T3G2A1C0T1C2'A3'T4'-3' and to the palindromic ATF/CREB sequence 5'-A4T3G2A1-C0*G0'T1'C2'A3'T4'-3'; C/EBP preferentially recognizes the palindromic sequence 5'-A4T3T2G1C0*G0'C1'A2'-A3'T4'-3...

journal_title：Nucleic acids research

authors： Koldin B,Suckow M,Seydel A,von Wilcken-Bergmann B,Müller-Hill B

更新日期：1995-10-25 00:00:00

abstract：:EcoRI digestion of total mouse DNA yields a prominant 1.3 kb fragment amounting to between 1 and 2% of the mouse genome. The majority of the 1.3 kb EcoRI fragments have a single Bg1II site 800 bp from one end. This EcoRI-Bg1II sequence family shows HindIII and HaeIII sequence heterogeneity. We have cloned representati...

journal_title：Nucleic acids research

authors： Heller R,Arnheim N

更新日期：1980-11-11 00:00:00

abstract：:Leishmania species are protozoan parasites whose remarkably plastic genome limits the establishment of effective genetic manipulation and leishmaniasis treatment. The strategies used by Leishmania to maintain its genome while allowing variability are not fully understood. Here, we used DiCre-mediated conditional gene ...

journal_title：Nucleic acids research

authors： Damasceno JD,Obonaga R,Silva GLA,Reis-Cunha JL,Duncan SM,Bartholomeu DC,Mottram JC,McCulloch R,Tosi LRO

更新日期：2018-12-14 00:00:00