Abstract:
:Three clones of non-repetitive sequences and six clones containing repetitive sequences were obtained from micronuclear DNA of Tetrahymena thermophila. All the non-repetitive and three repetitive sequences had the same organization in micro- and macronuclear DNAs as revealed by blot hybridization. On the other hand, the remaining three clones with repetitive sequences had apparently different organization in the two nuclear DNAs. All these repetitive sequences showed a smear on the blot in addition to a number of discrete bands when micronuclear DNA was digested with EcoR I. In macronuclear DNAs, these sequences invariably became one or two bands and the smear disappeared. We conclude that, when a macronucleus develops from a micronucleus, the non-repetitive sequences amplify by more than 20 times with relatively few rearrangement, whereas some selected portions of repeated and/or repeat-contiguous sequences are amplified with rather extensive reorganization.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Iwamura Y,Sakai M,Muramatsu Mdoi
10.1093/nar/10.14.4279subject
Has Abstractpub_date
1982-07-24 00:00:00pages
4279-91issue
14eissn
0305-1048issn
1362-4962journal_volume
10pub_type
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