Abstract：
:Two enzymatic pathways are involved in the inhibitory effects of double-stranded (ds)RNA on protein synthesis in cell extracts derived from interferon-treated human fibroblasts or HeLa cells, an oligonucleotide polymerase that synthesizes (2'-5')An from ATP and a protein kinase that phosphorylates the alpha subunit of initiation factor eIF-2 as well as a polypeptide of Mr = 72,000. We have now evaluated the activation of both the (2'-5')An polymerase and protein kinase by a large variety of polynucleotides, triple-stranded and synthetic dsRNAs, homopolymers, alternating copolymers, triple-stranded polymers, purine-purine duplexes and purine-pyrimidine duplexes with modifications at either the pyrimidine or ribose moieties. All these polynucleotides have been the subject of previous interferon induction studies. Some polynucleotides, i.e. (I)n.(C)n and mycophage dsRNA, which have been recognized as excellent interferon inducers, were also potent activators of both (2'-5')An polymerase and protein kinase, whereas non-inducers such as (A)n. (X)n and (A)n. (br5U)n did not activate either the kinase or the polymerase. However, some polymers like (I)n.(br5C)n, (difl)n(C)n and (dIcl)n (C)n, while potent interferon inducers and kinase activators, behaved poorly as activators of the (2'-5')An polymerase. Other polymers, i.e. (dAfl)n (U)n and (A)n.(U)nl (I)n, that do not induce interferon, activated the kinase but not the polymerase. Finally, (I)n (s2c)n, a relatively potent interferon inducer, did not activate either kinase or polymerase. These findings indicate that there is no simple relationship between the interferon-inducing ability of dsRNAs and their stimulating effects on (2'-5')An polymerase and protein kinase activity.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Baglioni C,Minks MA,De Clercq Edoi
10.1093/nar/9.19.4939subject
Has Abstractpub_date
1981-10-10 00:00:00pages
4939-50issue
19eissn
0305-1048issn
1362-4962journal_volume
9pub_type
杂志文章abstract：:UMSBP is a CCHC-type zinc finger protein, which functions during replication initiation of kinetoplast DNA minicircles and the segregation of kinetoplast DNA networks. Interactions of UMSBP with origin sequences, as well as the protein oligomerization, are affected by its redox state. Reduction yields UMSBP monomers a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn927
更新日期：2009-01-01 00:00:00
abstract：:We have reported that an upstream stimulatory factor (USF) binding site is functional in transcription of the heme oxygenase-1 gene. In this study, we examined the role of USF in the induced state. By transient expression analyses with the chloramphenicol acetyl-transferase gene, we found that the USF binding site pla...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.15.2959
更新日期：1996-08-01 00:00:00
abstract：:The nucleotide sequence of the control region of the divergent argECBH operon has been established in the wild type and in mutants affecting expression of these genes. The argE and argCBH promoters face each other and overlap with an operator region containing two domains which may act as distinct repressor binding si...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.24.8031
更新日期：1982-12-20 00:00:00
abstract：:Given the many roles proposed for Mg2+ in nucleic acids, it is essential to accurately determine their binding modes. Here, we surveyed the PDB to classify Mg2+ inner-sphere binding patterns to nucleobase imine N1/N3/N7 atoms. Among those, purine N7 atoms are considered to be the best nucleobase binding sites for diva...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1175
更新日期：2017-01-25 00:00:00
abstract：:A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity and biological activity studies of TBA variants modified by unlocked nucleic acid (UNA) monomers. UNA-U placed in position U3,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq823
更新日期：2011-02-01 00:00:00
abstract：:We observed overexpression and increased intra-nuclear accumulation of the PRMT5/WDR77 in breast cancer cell lines relative to immortalized breast epithelial cells. Utilizing mass spectrometry and biochemistry approaches we identified the Zn-finger protein ZNF326, as a novel interaction partner and substrate of the nu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx727
更新日期：2017-11-02 00:00:00
abstract：:Polyketide synthases (PKSs) catalyze biosynthesis of a diverse family of pharmaceutically important secondary metabolites. Bioinformatics analysis of sequence and structural features of PKS proteins plays a crucial role in discovery of new natural products by genome mining, as well as in design of novel secondary meta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq340
更新日期：2010-07-01 00:00:00
abstract：:The single nucleotide polymorphisms (SNPs) in conserved protein regions have been thought to be strong candidates that alter protein functions. Thus, we have developed SNP@Domain, a web resource, to identify SNPs within human protein domains. We annotated SNPs from dbSNP with protein structure-based as well as sequenc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl323
更新日期：2006-07-01 00:00:00
abstract：:From gene expression to nanotechnology, understanding and controlling DNA requires a detailed knowledge of its higher order structure and dynamics. Here we take advantage of the environment-sensitive photoisomerization of cyanine dyes to probe local and global changes in DNA structure. We report that a covalently atta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky887
更新日期：2018-11-30 00:00:00
abstract：:A peculiar phenomenon is observed in several adenovirus type 2 or 5 (Ad2 or Ad5) transformed cell lines: the right hand and left hand terminal regions of the viral genome present in the viral DNA insertions of these cell lines are found to be linked together. A large part of the viral DNA insertion present in the Ad5 ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.7.2189
更新日期：1982-04-10 00:00:00
abstract：:The DINAMelt web server simulates the melting of one or two single-stranded nucleic acids in solution. The goal is to predict not just a melting temperature for a hybridized pair of nucleic acids, but entire equilibrium melting profiles as a function of temperature. The two molecules are not required to be complementa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki591
更新日期：2005-07-01 00:00:00
abstract：:MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides, which negatively regulate the gene expression at the post-transcriptional level. This study describes an update of the miRTarBase (http://miRTarBase.mbc.nctu.edu.tw/) that provides information about experimentally validated miRNA-target inte...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1258
更新日期：2016-01-04 00:00:00
abstract：:The protein property prediction and testing database (PPT-DB) is a database housing nearly 30 carefully curated databases, each of which contains commonly predicted protein property information. These properties include both structural (i.e. secondary structure, contact order, disulfide pairing) and dynamic (i.e. orde...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm800
更新日期：2008-01-01 00:00:00
abstract：:Replication factor C (RF-C) is a eukaryotic heteropentameric protein required for DNA replication and repair processes by loading proliferating cell nuclear antigen (PCNA) onto DNA in an ATP-dependent manner. Prior to loading PCNA, RF-C binds to DNA. This binding is thought to be restricted to a specific DNA structure...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.17.3433
更新日期：1999-09-01 00:00:00
abstract：:An enzyme capable of liberating functional tRNA(Lys) from Escherichia coli diacetyl-lysyl-tRNA(Lys) was purified from the archae Sulfolobus solfataricus. Contrasting with the specificity of peptidyl- tRNA hydrolase (PTH) from E.coli, the S.solfataricus enzyme readily accepts E.coli formyl-methionyl-tRNA(fMet) as a sub...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg428
更新日期：2003-06-15 00:00:00
abstract：:Neuroserpin is an axonally secreted serine protease inhibitor expressed in the nervous system that protects neurons from ischemia-induced apoptosis. Mutant neuroserpin forms have been found polymerized in inclusion bodies in a familial autosomal encephalopathy causing dementia, or associated with epilepsy. Regulation ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.10.2202
更新日期：2002-05-15 00:00:00
abstract：:The genetic etiology of premature ovarian insufficiency (POI) has been well established to date, however, the role of long noncoding RNAs (lncRNAs) in POI is largely unknown. In this study, we identified a down-expressed lncRNA HCP5 in granulosa cells (GCs) from biochemical POI (bPOI) patients, which impaired DNA dama...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa127
更新日期：2020-05-07 00:00:00
abstract：:Restriction fragments of T7 DNA which selectively bind E. coli RNA polymerase have been identified. These include fragments located close to the beginning of gene 1 where according to Minkley and Pribnow (1973) there is a promoter called C. The smallest fragment from this region which binds RNA polymerase has been seq...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.2.525
更新日期：1979-02-01 00:00:00
abstract：:We have isolated and characterized DNA probes that detect homologies between the X and Y chromosomes. Clone St25 is derived from the q13-q22 region of the X chromosome and recognizes a 98% homologous sequence on the Y chromosome. Y specific fragments were present in DNAs from 5 Yq-individuals and from 4 out of 7 XX ma...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.15.5485
更新日期：1985-08-12 00:00:00
abstract：:We describe LincSNP 2.0 (http://bioinfo.hrbmu.edu.cn/LincSNP), an updated database that is used specifically to store and annotate disease-associated single nucleotide polymorphisms (SNPs) in human long non-coding RNAs (lncRNAs) and their transcription factor binding sites (TFBSs). In LincSNP 2.0, we have updated the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw945
更新日期：2017-01-04 00:00:00
abstract：:Aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are importan...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv152
更新日期：2015-04-20 00:00:00
abstract：:High density oligonucleotide array technology is widely used in many areas of biomedical research for quantitative and highly parallel measurements of gene expression. Affymetrix GeneChip arrays are the most popular. In this technology each gene is typically represented by a set of 11-20 pairs of probes. In order to o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gng015
更新日期：2003-02-15 00:00:00
abstract：:Evidence is presented that the editing mechanisms of aminoacyl-tRNA synthetase operate by two alternative pathways: pre-transfer, by hydrolysis of the non-cognate aminoacyl adenylate; post-transfer, by hydrolysis of the mischarged tRNA. The methionyl-tRNA synthetases from Escherichia coli and Bacillus stearothermophil...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.13.3105
更新日期：1981-07-10 00:00:00
abstract：:Transcription is the first step in gene expression, and it is the step at which most of the regulation of expression occurs. Although sequenced prokaryotic genomes provide a wealth of information, transcriptional regulatory networks are still poorly understood using the available genomic information, largely because a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr795
更新日期：2012-02-01 00:00:00
abstract：:RNA levels in a cell are regulated by the relative rates of RNA synthesis and decay. We recently developed a new approach for measuring both RNA synthesis and decay in a single experimental setting by biosynthetic labeling of newly transcribed RNA. Here, we show that this provides measurements of RNA half-lives from m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp542
更新日期：2009-09-01 00:00:00
abstract：:High performance liquid chromatography analysis of imidazole open ring 7-methylguanine, 2-6 diamino-4-hydroxy-5N-methyl-formamidopyrimidine (rom7G), showed two well-separated peaks (fI and fII) of the same magnitude. Rechromatography of each isolated component indicated that they are slowly interconverted to give a 1:...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.13.5429
更新日期：1984-07-11 00:00:00
abstract：:Direct labeling of RNA is an expedient method for labeling large quantities (e.g. micrograms) of target RNA for microarray analysis. We have developed an efficient labeling system that uses T4 RNA ligase to attach a 3'-biotinylated donor molecule to target RNA. Microarray analyses indicate that directly labeled RNA is...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh085
更新日期：2004-06-17 00:00:00
abstract：:Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well as inter- and intra-cellular fluctuations in gene expression tha...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz181
更新日期：2019-05-21 00:00:00
abstract：:TGGCA-binding proteins are nuclear proteins with high affinity for double-stranded DNA homologous to the prototype recognition sequence 5'YTGGCANNNTGCCAR 3'. Their ubiquitous tissue distribution in higher vertebrates characterizes them as a class of highly conserved proteins which may exert a basic function. To obtain...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.6.2045
更新日期：1985-03-25 00:00:00
abstract：:The human 5S rRNA genes are found in clusters of tandem repeated units. We have cloned and partially characterized six restriction fragments from two clusters of 2.3 kb and 1.6 kb repeats, respectively. Four fragments from the cluster of 2.3 kb repeats contain a 5S rRNA gene and one fragment contains a gene variant wi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.15.4147
更新日期：1991-08-11 00:00:00
