Abstract:
:The human 5S rRNA genes are found in clusters of tandem repeated units. We have cloned and partially characterized six restriction fragments from two clusters of 2.3 kb and 1.6 kb repeats, respectively. Four fragments from the cluster of 2.3 kb repeats contain a 5S rRNA gene and one fragment contains a gene variant with an additional nucleotide in the internal control region. A fragment from the 1.6 kb cluster contains a gene and is highly homologous to the 2.3 kb repeats, except for a large deletion in the 3'-flanking region starting 12 bp downstream of the gene. The number of genes and closely related gene variants is found to be 300-400 per haploid human genome. 100-150 of these are found in 2.3 kb repeats and 5-10 are found in 1.6 kb repeats. The total number of 5S rRNA sequences, including pseudogenes, is 1700-2000 per haploid genome. The genes and the gene variant are transcribed equally efficient in a HeLa cell extract. If 5'-flanking sequences, including a GC-motif in the -40 region, are removed from the genes, transcription is reduced with a factor 10 or more, suggesting that sequences upstream of the coding region are important for the level of transcription.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sørensen PD,Frederiksen Sdoi
10.1093/nar/19.15.4147subject
Has Abstractpub_date
1991-08-11 00:00:00pages
4147-51issue
15eissn
0305-1048issn
1362-4962journal_volume
19pub_type
杂志文章abstract::alpha2-Macroglobulin covalently linked to poly(L)-lysine can be used as a vehicle for receptor-mediated gene transfer. This modified alpha2-macroglobulin maintains its ability to bind to the alpha2-macroglobulin receptor, and was shown to introduce a luciferase reporter gene plasmid into HepG2 human hepatoma cells in ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.19.3873
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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更新日期:2009-11-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2020-05-07 00:00:00
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journal_title:Nucleic acids research
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更新日期:1982-10-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
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更新日期:2006-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2001-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.16.3327
更新日期:2001-08-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks327
更新日期:2012-08-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2001-04-15 00:00:00
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更新日期:2018-07-02 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.3.470
更新日期:1996-02-01 00:00:00
abstract::Lambda-red system-based recombinogenic engineering is a powerful new method to engineer DNA without the need for restriction enzymes or ligases. Here, we report the use of a single selectable marker to enhance the usefulness of this approach. The strategy is to utilize the thymidylate synthase A (thyA) gene, which enc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gni059
更新日期:2005-03-30 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1990-11-25 00:00:00
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journal_title:Nucleic acids research
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更新日期:2000-06-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1997-09-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq808
更新日期:2011-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.20.5297
更新日期:1981-10-24 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.10.3375
更新日期:1983-05-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt664
更新日期:2013-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.13.3297
更新日期:1992-07-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn584
更新日期:2008-10-01 00:00:00
abstract::Rad54 protein is a key member of the RAD52 epistasis group required for homologous recombination in eukaryotes. Rad54 is a duplex DNA translocase that remodels both DNA and protein-DNA complexes, and functions at multiple steps in the recombination process. Here we use biochemical criteria to demonstrate the existence...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp068
更新日期:2009-05-01 00:00:00