Abstract:
:A peculiar phenomenon is observed in several adenovirus type 2 or 5 (Ad2 or Ad5) transformed cell lines: the right hand and left hand terminal regions of the viral genome present in the viral DNA insertions of these cell lines are found to be linked together. A large part of the viral DNA insertion present in the Ad5 transformed rat cell line 5RK20 has been cloned in the lambda vector Charon21A, including the segment containing the linked terminal regions. Sequence analysis of the linkage region showed a perfect homology with the Ad5 DNA sequence and a direct linkage of basepair (bp) 63 of the left hand end of the viral genome to bp 108 of the right hand end. No cellular or rearranged viral sequences were present. Our findings suggest that the joining of viral sequences into the cellular genome.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Visser L,Reemst AC,van Mansfeld AD,Rozijn THdoi
10.1093/nar/10.7.2189subject
Has Abstractpub_date
1982-04-10 00:00:00pages
2189-98issue
7eissn
0305-1048issn
1362-4962journal_volume
10pub_type
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