Nucleotide sequence analysis of the linked left and right hand terminal regions of adenovirus type 5 DNA present in the transformed rat cell line 5RK20.

Abstract:

:A peculiar phenomenon is observed in several adenovirus type 2 or 5 (Ad2 or Ad5) transformed cell lines: the right hand and left hand terminal regions of the viral genome present in the viral DNA insertions of these cell lines are found to be linked together. A large part of the viral DNA insertion present in the Ad5 transformed rat cell line 5RK20 has been cloned in the lambda vector Charon21A, including the segment containing the linked terminal regions. Sequence analysis of the linkage region showed a perfect homology with the Ad5 DNA sequence and a direct linkage of basepair (bp) 63 of the left hand end of the viral genome to bp 108 of the right hand end. No cellular or rearranged viral sequences were present. Our findings suggest that the joining of viral sequences into the cellular genome.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Visser L,Reemst AC,van Mansfeld AD,Rozijn TH

doi

10.1093/nar/10.7.2189

subject

Has Abstract

pub_date

1982-04-10 00:00:00

pages

2189-98

issue

7

eissn

0305-1048

issn

1362-4962

journal_volume

10

pub_type

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