Summaries of Affymetrix GeneChip probe level data.

abstract：:Alternative splicing has recently emerged as a major mechanism of regulation in the human genome, occurring in perhaps 40-60% of human genes. Thus, microarray studies of functional regulation could, in principle, be extended to detect not only the changes in the overall expression of a gene, but also changes in its sp...

journal_title：Nucleic acids research

authors： Le K,Mitsouras K,Roy M,Wang Q,Xu Q,Nelson SF,Lee C

更新日期：2004-12-14 00:00:00

abstract：:The products of many bacterial non-ribosomal peptide synthetases (NRPS) are highly important secondary metabolites, including vancomycin and other antibiotics. The ability to predict substrate specificity of newly detected NRPS Adenylation (A-) domains by genome sequencing efforts is of great importance to identify an...

journal_title：Nucleic acids research

authors： Röttig M,Medema MH,Blin K,Weber T,Rausch C,Kohlbacher O

更新日期：2011-07-01 00:00:00

abstract：:IntAct is an open-source, open data molecular interaction database populated by data either curated from the literature or from direct data depositions. Two levels of curation are now available within the database, with both IMEx-level annotation and less detailed MIMIx-compatible entries currently supported. As from ...

journal_title：Nucleic acids research

authors： Kerrien S,Aranda B,Breuza L,Bridge A,Broackes-Carter F,Chen C,Duesbury M,Dumousseau M,Feuermann M,Hinz U,Jandrasits C,Jimenez RC,Khadake J,Mahadevan U,Masson P,Pedruzzi I,Pfeiffenberger E,Porras P,Raghunath A,Roeche

更新日期：2012-01-01 00:00:00

abstract：:Genetic recombination in meiosis plays an important role in generating diversity of genetic information. In yeast an Escherichia coli RecA-like gene, DMC1, is expressed in meiotic prophase and its product co-localizes with Rad51 protein on zygotene chromosomes. We have cloned the mouse and human homologs of the yeast ...

journal_title：Nucleic acids research

authors： Habu T,Taki T,West A,Nishimune Y,Morita T

更新日期：1996-02-01 00:00:00

abstract：:While it has been long recognized that genes are not randomly positioned along the genome, the degree to which its 3D structure influences the arrangement of genes has remained elusive. In particular, several lines of evidence suggest that actively transcribed genes are spatially co-localized, forming transcription fa...

journal_title：Nucleic acids research

authors： Ben-Elazar S,Yakhini Z,Yanai I

更新日期：2013-02-01 00:00:00

abstract：:The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5'- and 3'-non-coding region contains sev...

journal_title：Nucleic acids research

authors： Ledeboer AM,Edens L,Maat J,Visser C,Bos JW,Verrips CT,Janowicz Z,Eckart M,Roggenkamp R,Hollenberg CP

更新日期：1985-05-10 00:00:00

abstract：:The initiation region of the MS2 replicase cistron can be isolated as a fragment 59 bases in length protected from RNAase by the binding of the coat protein which serves as a translational repressor. This fragment MS2 R(-53 leads to 6) starts 53 bases before the initiation codon and retains full activity in binding ri...

journal_title：Nucleic acids research

authors： Borisova GP,Volkova TM,Berzin V,Rosenthal G,Gren EJ

更新日期：1979-01-01 00:00:00

abstract：:mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Us...

journal_title：Nucleic acids research

authors： Gupta R,Thomas P,Beddington RS,Rigby PW

更新日期：1998-10-01 00:00:00

abstract：:WorfDB (Worm ORFeome DataBase; http://worfdb.dfci.harvard.edu) was created to integrate and disseminate the data from the cloning of complete set of approximately 19 000 predicted protein-encoding Open Reading Frames (ORFs) of Caenorhabditis elegans (also referred to as the 'worm ORFeome'). WorfDB serves as a central ...

journal_title：Nucleic acids research

authors： Vaglio P,Lamesch P,Reboul J,Rual JF,Martinez M,Hill D,Vidal M

更新日期：2003-01-01 00:00:00

abstract：:The mouse is widely used as system to study human genetic mechanisms. However, extensive rewiring of transcriptional regulatory networks often confounds translation of findings between human and mouse. Site-specific gain and loss of individual transcription factor binding sites (TFBS) has caused functional divergence ...

journal_title：Nucleic acids research

authors： Diehl AG,Boyle AP

更新日期：2018-02-28 00:00:00

abstract：:Loss-of-function (LOF) methods such as RNA interference (RNAi), antisense oligonucleotides or CRISPR-based genome editing provide unparalleled power for studying the biological function of genes of interest. However, a major concern is non-specific targeting, which involves depletion of transcripts other than those in...

journal_title：Nucleic acids research

authors： Stojic L,Lun ATL,Mangei J,Mascalchi P,Quarantotti V,Barr AR,Bakal C,Marioni JC,Gergely F,Odom DT

更新日期：2018-07-06 00:00:00

abstract：:Integration of foreign genes into plant genomes by the Agrobacterium T-DNA transfer system has been considered to occur at random. It has been speculated that the chromosomal structure of the integration site might affect the expression pattern of the introduced genes. To gain insight into the molecular structure of T...

journal_title：Nucleic acids research

authors： Dietz A,Kay V,Schlake T,Landsmann J,Bode J

更新日期：1994-07-25 00:00:00

abstract：:The molecular mechanism in pancreatic β cells underlying hyperlipidemia and insulin insufficiency remains unclear. Here, we find that the fatty acid-induced decrease in insulin levels occurs due to a decrease in insulin translation. Since regulation at the translational level is generally mediated through RNA-binding ...

journal_title：Nucleic acids research

authors： Li Z,Zhou M,Cai Z,Liu H,Zhong W,Hao Q,Cheng D,Hu X,Hou J,Xu P,Xue Y,Zhou Y,Xu T

更新日期：2018-12-14 00:00:00

abstract：:Hybridization solutions containing chaotropes may be used to modulate the thermal stability (Tm or Td) of oligodeoxynucleotide (ODN) duplexes or hybrids over a 90 degrees C range. Modulation of Td allows formulation of hybridization solutions that permit ambient temperature hybridization using most combinations of pro...

journal_title：Nucleic acids research

authors： Van Ness J,Chen L

更新日期：1991-10-11 00:00:00

abstract：:Xenopus laevis 5S rRNA isolated from 7S particles or transcribed in vitro is found to adopt two alternative conformations. These two conformers contain different structural elements within the major TF III A binding domain, which, when isolated as RNA fragments, still interact with the transcription factor. Chemical m...

journal_title：Nucleic acids research

authors： Pieler T,Guddat U,Oei SL,Erdmann VA

更新日期：1986-08-11 00:00:00

abstract：:A simplified and economical method for the attachment of 2'-deoxyribo, ribo and arabinonucleosides onto long-chain alkylamidopropanoic acid controlled-pore glass (LCAAP-CPG, P-3) is described. In this procedure, 5'-O-tritylated nucleosides are coupled directly to LCAAP-CPG in excellent yields using 1-(3-dimethylaminop...

journal_title：Nucleic acids research

authors： Damha MJ,Giannaris PA,Zabarylo SV

更新日期：1990-07-11 00:00:00

abstract：:Riboswitch RNAs fold into complex tertiary structures upon binding to their cognate ligand. Ligand recognition is accomplished by key residues in the binding pocket. In addition, it often crucially depends on the stability of peripheral structural elements. The ligand-bound complex of the guanine-sensing riboswitch fr...

journal_title：Nucleic acids research

authors： Buck J,Wacker A,Warkentin E,Wöhnert J,Wirmer-Bartoschek J,Schwalbe H

更新日期：2011-12-01 00:00:00

abstract：:Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metallothionein (MT) gene in adult mouse liver, when induced to produce mRNA by injection of cadmium, becomes more susceptible to nuc...

journal_title：Nucleic acids research

authors： Koropatnick J,Andrews G,Duerksen JD,Varshney U,Gedamu L

更新日期：1983-05-25 00:00:00

abstract：:We updated the plant transcription factor (TF) database to version 2.0 (PlantTFDB 2.0, http://planttfdb.cbi.pku.edu.cn) which contains 53,319 putative TFs predicted from 49 species. We made detailed annotation including general information, domain feature, gene ontology, expression pattern and ortholog groups, as well...

journal_title：Nucleic acids research

authors： Zhang H,Jin J,Tang L,Zhao Y,Gu X,Gao G,Luo J

更新日期：2011-01-01 00:00:00

abstract：:RegulonDB is a DataBase that integrates biological knowledge of the mechanisms that regulate the transcription initiation in Escherichia coli , as well as knowledge on the organization of the genes and regulatory signals into operons in the chromosome. The operon is the basic structure used in RegulonDB to describe th...

journal_title：Nucleic acids research

authors： Huerta AM,Salgado H,Thieffry D,Collado-Vides J

更新日期：1998-01-01 00:00:00

abstract：:We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the human papillomavirus E6...

journal_title：Nucleic acids research

authors： Liu WL,Stevenson M,Seymour LW,Fisher KD

更新日期：2009-01-01 00:00:00

abstract：:Bgee is a database to retrieve and compare gene expression patterns in multiple animal species, produced by integrating multiple data types (RNA-Seq, Affymetrix, in situ hybridization, and EST data). It is based exclusively on curated healthy wild-type expression data (e.g., no gene knock-out, no treatment, no disease...

journal_title：Nucleic acids research

authors： Bastian FB,Roux J,Niknejad A,Comte A,Fonseca Costa SS,de Farias TM,Moretti S,Parmentier G,de Laval VR,Rosikiewicz M,Wollbrett J,Echchiki A,Escoriza A,Gharib WH,Gonzales-Porta M,Jarosz Y,Laurenczy B,Moret P,Person E,

更新日期：2021-01-08 00:00:00

abstract：:The Intronerator (http://www.cse.ucsc.edu/ approximately kent/intronerator/ ) is a set of web-based tools for exploring RNA splicing and gene structure in Caenorhabditis elegans. It includes a display of cDNA alignments with the genomic sequence, a catalog of alternatively spliced genes and a database of introns. The ...

journal_title：Nucleic acids research

authors： Kent WJ,Zahler AM

更新日期：2000-01-01 00:00:00

abstract：:Ribosome synthesis involves the concomitance of pre-rRNA processing and ribosomal protein assembly. In eukaryotes, this is a complex process that requires the participation of specific sequences and structures within the pre-rRNAs, at least 200 trans-acting factors and the ribosomal proteins. There is little informati...

journal_title：Nucleic acids research

authors： Babiano R,de la Cruz J

更新日期：2010-08-01 00:00:00

abstract：:Originally identified as an RNA polymerase II interactor, Che-1/AATF (Che-1) has now been recognized as a multifunctional protein involved in cell-cycle regulation and cancer progression, as well as apoptosis inhibition and response to stress. This protein displays a peculiar nucleolar localization and it has recently...

journal_title：Nucleic acids research

authors： Sorino C,Catena V,Bruno T,De Nicola F,Scalera S,Bossi G,Fabretti F,Mano M,De Smaele E,Fanciulli M,Iezzi S

更新日期：2020-06-19 00:00:00

abstract：:The nucleotide sequence of the portion of a Bacillus subtilis (strain PAP115) 3 kb Pst I fragment which contains an endo-beta-1, 4-glucanase gene has been determined. This gene encodes a protein of 499 amino acid residues (Mr = 55,234) with a typical B. subtilis signal peptide. Escherichia coli which has been transfor...

journal_title：Nucleic acids research

authors： MacKay RM,Lo A,Willick G,Zuker M,Baird S,Dove M,Moranelli F,Seligy V

更新日期：1986-11-25 00:00:00

abstract：:The nucleobase modification 5-methylcytosine (m(5)C) is widespread both in DNA and different cellular RNAs. The functions and enzymatic mechanisms of DNA m(5)C-methylation were extensively studied during the last decades. However, the location, the mechanism of formation and the cellular function(s) of the same modifi...

journal_title：Nucleic acids research

authors： Motorin Y,Lyko F,Helm M

更新日期：2010-03-01 00:00:00

abstract：:The mRNA encoding repressor cI of phage lambda is the only known E. coli message which starts directly with the initiation AUG codon. The ability of in vitro synthesized cI mRNA fragments (150 or 400 nts) to form ternary initiation complexes has been studied using the toeprint method. In the presence of tRNA(Met)f, th...

journal_title：Nucleic acids research

authors： Balakin AG,Skripkin EA,Shatsky IN,Bogdanov AA

更新日期：1992-02-11 00:00:00

abstract：:Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented DNA, information traditionally lost in standard NGS library prepa...

journal_title：Nucleic acids research

authors： Harkins KM,Schaefer NK,Troll CJ,Rao V,Kapp J,Naughton C,Shapiro B,Green RE

更新日期：2020-05-07 00:00:00

abstract：:The genomic distribution of 23 nuclear genes from three dicotyledons (pea, sunflower, tobacco) and five monocotyledons of the Gramineae family (barley, maize, rice, oat, wheat) was studied by localizing these genes in DNA fractions obtained by preparative centrifugation in Cs2SO4/BAMD density gradients. Each one of th...

journal_title：Nucleic acids research

authors： Montero LM,Salinas J,Matassi G,Bernardi G

更新日期：1990-04-11 00:00:00