A plant scaffold attached region detected close to a T-DNA integration site is active in mammalian cells.

abstract：:The sequential arrangement of histones along DNA in nucleosome core particles was determined between 0.5 and 600 mM salt and from 0 to 8 M urea. These concentrations of salt and urea up to 6 M had no significant effect on the linear order of histones along DNA but 8 M urea caused the rearrangement of histones. Conform...

journal_title：Nucleic acids research

authors： Zayetz VW,Bavykin SG,Karpov VL,Mirzabekov AD

更新日期：1981-03-11 00:00:00

abstract：:Mammalian DNA (cytosine-5) methyltransferase 1 (DNMT1) is essential for maintenance methylation. Phosphorylation of Ser143 (pSer143) stabilizes DNMT1 during DNA replication. Here, we show 14-3-3 is a reader protein of DNMT1pSer143. In mammalian cells 14-3-3 colocalizes and binds DNMT1pSer143 post-DNA replication. The ...

journal_title：Nucleic acids research

authors： Estève PO,Zhang G,Ponnaluri VK,Deepti K,Chin HG,Dai N,Sagum C,Black K,Corrêa IR Jr,Bedford MT,Cheng X,Pradhan S

更新日期：2016-02-29 00:00:00

abstract：:The convergently transcribed restriction (R) and methylase (M) genes of the Restriction-Modification system Esp1396I are tightly regulated by a controller (C) protein that forms part of the CR operon. We have mapped the transcriptional start sites from each promoter and examined the regulatory role of C.Esp1396I in vi...

journal_title：Nucleic acids research

authors： Bogdanova E,Zakharova M,Streeter S,Taylor J,Heyduk T,Kneale G,Severinov K

更新日期：2009-06-01 00:00:00

abstract：:Search of interrelationships between the structural-functional protein organization and exon structure of encoding gene provides insights into issues concerned with the function, origin and evolution of genes and proteins. The functions of proteins and their domains are defined mostly by functional sites. The relation...

journal_title：Nucleic acids research

authors： Medvedeva I,Demenkov P,Kolchanov N,Ivanisenko V

更新日期：2012-01-01 00:00:00

abstract：:Selected groups of isolated 14C-labelled proteins from E. coli 30S ribosomal subunits were reconstituted with 32P-labelled 16S RNA, and the reconstituted complexes were partially digested with ribonuclease A. RNA fragments protected by the proteins were separated by gel electrophoresis and subjected to sequence analys...

journal_title：Nucleic acids research

authors： Wiener L,Schüler D,Brimacombe R

更新日期：1988-02-25 00:00:00

abstract：:In many eukaryotic mRNAs one or more short 'upstream' open reading frames, uORFs, precede the initiator of the main coding sequence. Upstream ORFs are functionally diverse as illustrated by their variety of features in polyamine pathway biosynthetic mRNAs. Their propensity to act as sensors for regulatory circuits and...

journal_title：Nucleic acids research

authors： Ivanov IP,Atkins JF,Michael AJ

更新日期：2010-01-01 00:00:00

abstract：:A typical G-rich telomeric DNA strand, which runs 5'-->3' toward the chromosome ends, protrudes by several nucleotides in lower eukaryotes. In human chromosomes long G-rich 3'-overhangs have been found. Apart from the standard G-rich tail, several non-canonical terminal structures have been proposed. However, the mech...

journal_title：Nucleic acids research

authors： Cimino-Reale G,Pascale E,Battiloro E,Starace G,Verna R,D'Ambrosio E

更新日期：2001-04-01 00:00:00

abstract：:Eight base analogs were tested as third strand residues in otherwise homopyrimidine strands opposite each of the 'direct' (A.T and G.C) and 'inverted' (T.A and C.G) Watson-Crick base pairs, using UV melting profiles to assess triplex stability. The target duplexes contained 20 A.T base pairs and a central test base pa...

journal_title：Nucleic acids research

authors： Amosova OA,Fresco JR

更新日期：1999-12-01 00:00:00

abstract：:A minizyme is a hammerhead ribozyme with short oligonucleotide linkers instead of stem-loop II. In a previous study we demonstrated that a minizyme with high activity forms a dimeric structure with a common stem II. Because of their dimeric structure, minizymes are potentially capable of cleaving a substrate at two di...

journal_title：Nucleic acids research

authors： Kuwabara T,Amontov SV,Warashina M,Ohkawa J,Taira K

更新日期：1996-06-15 00:00:00

abstract：:The goal of this study was to address antiparallel triplex formation at duplex targets that do not conform to a strict oligopurine.oligopyrimidine motif. We focused on the ability of natural bases and base analogs incorporated into oligonucleotide third strands to bind to so-called CG inversions. These are sites where...

journal_title：Nucleic acids research

authors： Durland RH,Rao TS,Revankar GR,Tinsley JH,Myrick MA,Seth DM,Rayford J,Singh P,Jayaraman K

更新日期：1994-08-11 00:00:00

abstract：:We demonstrate a single DNA molecule optical mapping assay able to resolve a specific Escherichia coli strain from other strains. The assay is based on competitive binding of the fluorescent dye YOYO-1 and the AT-specific antibiotic netropsin. The optical map is visualized by stretching the DNA molecules in nanofluidi...

journal_title：Nucleic acids research

authors： Nilsson AN,Emilsson G,Nyberg LK,Noble C,Stadler LS,Fritzsche J,Moore ER,Tegenfeldt JO,Ambjörnsson T,Westerlund F

更新日期：2014-09-01 00:00:00

abstract：:Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing tran...

journal_title：Nucleic acids research

authors： Sparrow DB,Latinkic B,Mohun TJ

更新日期：2000-02-15 00:00:00

abstract：:The Yeast Resource Center Public Data Repository (YRC PDR) serves as a single point of access for the experimental data produced from many collaborations typically studying Saccharomyces cerevisiae (baker's yeast). The experimental data include large amounts of mass spectrometry results from protein co-purification ex...

journal_title：Nucleic acids research

authors： Riffle M,Malmström L,Davis TN

更新日期：2005-01-01 00:00:00

abstract：:We have investigated the distribution of mitochondrial DNA polymorphisms in a rare maternally transmitted genetic trait that causes hypersensitivity to aminoglycoside antibiotics, in the hope that a characterization of its molecular basis might provide a molecular and cellular understanding of aminoglycoside-induced d...

journal_title：Nucleic acids research

authors： Hutchin T,Haworth I,Higashi K,Fischel-Ghodsian N,Stoneking M,Saha N,Arnos C,Cortopassi G

更新日期：1993-09-11 00:00:00

abstract：:We had shown previously (3) that the E. coli argE gene could be turned-on by an IS3 element inserted in orientation II near the 5' end of the gene. Here we show that this effect is due to the presence of an outward promoter located on IS3. The exact site of insertion of IS3 was determined by DNA sequencing. Using the ...

journal_title：Nucleic acids research

authors： Charlier D,Piette J,Glansdorff N

更新日期：1982-10-11 00:00:00

abstract：:Trf4/5p-Air1/2p-Mtr4p polyadenylation complex (TRAMP) is an essential component of nuclear RNA surveillance in yeast. It recognizes a variety of nuclear transcripts produced by all three RNA polymerases, adds short poly(A) tails to aberrant or unstable RNAs and activates the exosome for their degradation. Despite the ...

journal_title：Nucleic acids research

authors： Holub P,Lalakova J,Cerna H,Pasulka J,Sarazova M,Hrazdilova K,Arce MS,Hobor F,Stefl R,Vanacova S

更新日期：2012-07-01 00:00:00

abstract：:This paper presents TurboFold II, an extension of the TurboFold algorithm for predicting secondary structures for multiple RNA homologs. TurboFold II augments the structure prediction capabilities of TurboFold by additionally providing multiple sequence alignments. Probabilities for alignment of nucleotide positions b...

journal_title：Nucleic acids research

authors： Tan Z,Fu Y,Sharma G,Mathews DH

更新日期：2017-11-16 00:00:00

abstract：:We have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine). Such treatment causes a substantial induction of DNA alkyltransferase activity, with the specific activity in treated cells increasing up to o...

journal_title：Nucleic acids research

authors： Baker SM,Margison GP,Strike P

更新日期：1992-02-25 00:00:00

abstract：:To explain biochemical and genetic data on spontaneous nucleotide replacements in nucleic acid biosynthesis all the 8 mispairs in normal tautomeric forms have been considered. Possible B-conformations of DNA fragments containing each of such mispairs incorporated between Watson-Crick pairs have been found using comput...

journal_title：Nucleic acids research

authors： Chuprina VP,Poltev VI

更新日期：1985-01-11 00:00:00

abstract：:By means of a combination of ion-exchange and sequence-specific affinity chromatography techniques, we have purified to homogeneity two protein complexes binding in a human DNA region (B48) previously recognized to contain a DNA replication origin. The DNA sequence used for the protein purification (B48 binding site) ...

journal_title：Nucleic acids research

authors： Tóth EC,Marusic L,Ochem A,Patthy A,Pongor S,Giacca M,Falaschi A

更新日期：1993-07-11 00:00:00

abstract：:Correlation of motif occurrences with gene expression intensity is an effective strategy for elucidating transcriptional cis-regulatory logic. Here we demonstrate that this approach can also identify cis-regulatory elements for alternative pre-mRNA splicing. Using data from a human exon microarray, we identified 56 ca...

journal_title：Nucleic acids research

authors： Das D,Clark TA,Schweitzer A,Yamamoto M,Marr H,Arribere J,Minovitsky S,Poliakov A,Dubchak I,Blume JE,Conboy JG

更新日期：2007-01-01 00:00:00

abstract：:The spliceosomal protein Prp1 (Prp6/U5-102 K) is necessary for the integrity of pre-catalytic spliceosomal complexes. We have identified a novel regulatory function for Prp1. Expression of mutations in the N-terminus of Prp1 leads to the accumulation of pre-catalytic spliceosomal complexes containing the five snRNAs U...

journal_title：Nucleic acids research

authors： Lützelberger M,Bottner CA,Schwelnus W,Zock-Emmenthal S,Razanau A,Käufer NF

更新日期：2010-03-01 00:00:00

abstract：:The mini-exon, a short segment found at the 5' end of trypanosome mRNAs, is contributed by a small RNA, the mini-exon donor (medRNA). In vivo 32P-labeled medRNA, a set of smaller RNAs related to it, and mRNA, were purified from Trypanosoma brucei by hybrid selection and gel electrophoresis. Using RNA fingerprinting an...

journal_title：Nucleic acids research

authors： Freistadt MS,Cross GA,Branch AD,Robertson HD

更新日期：1987-12-10 00:00:00

abstract：:Gut-enriched Krüppel-like factor (GKLF or KLF4) is a pleiotropic (activating and repressive) transcription factor. This study characterizes the mechanisms of transactivation by GKLF. Using a GAL4 fusion assay, the activating domain of murine GKLF was localized to the 109 amino acid residues in the N-terminus. Site-dir...

journal_title：Nucleic acids research

authors： Geiman DE,Ton-That H,Johnson JM,Yang VW

更新日期：2000-03-01 00:00:00

abstract：:Ornithine decarboxylase (ODC) expression is important for proliferation and is elevated in many tumor cells. We previously showed that Sp1 is a major positive regulator of ODC transcription. In this paper we have investigated transcriptional regulation of rat ODC by the closely related factor Sp3. While over-expressio...

journal_title：Nucleic acids research

authors： Kumar AP,Butler AP

更新日期：1997-05-15 00:00:00

abstract：:Deletion of Hop2 in mice eliminates homologous chromosome synapsis and disrupts double-strand break (DSB) repair through homologous recombination. HOP2 in vitro shows two distinctive activities: when it is incorporated into a HOP2-MND1 complex it stimulates DMC1 and RAD51 recombination activities and the purified HOP2...

journal_title：Nucleic acids research

authors： Pezza RJ,Voloshin ON,Volodin AA,Boateng KA,Bellani MA,Mazin AV,Camerini-Otero RD

更新日期：2014-02-01 00:00:00

abstract：:Nematode.net (http://nematode.net) is a publicly available resource dedicated to the study of parasitic nematodes. In 2000, the Genome Center at Washington University (GC) joined a consortium including the Nematode Genomics group in Edinburgh, and the Pathogen Sequencing Unit of the Sanger Institute to generate expres...

journal_title：Nucleic acids research

authors： Martin J,Abubucker S,Wylie T,Yin Y,Wang Z,Mitreva M

更新日期：2009-01-01 00:00:00

abstract：:As opposed to the vast majority of prokaryotic repressors, the immunity repressor of temperate Escherichia coli phage P2 (C) recognizes non-palindromic direct repeats of DNA rather than inverted repeats. We have determined the crystal structure of P2 C at 1.8 Å. This constitutes the first structure solved from the fam...

journal_title：Nucleic acids research

authors： Massad T,Skaar K,Nilsson H,Damberg P,Henriksson-Peltola P,Haggård-Ljungquist E,Högbom M,Stenmark P

更新日期：2010-11-01 00:00:00

abstract：:Allele-specific DNA methylation, histone acetylation and histone methylation are recognized as epigenetic characteristics of imprinted genes and imprinting centers (ICs). These epigenetic modifications are also used to regulate tissue-specific gene expression. Epigenetic differences between alleles can be significant ...

journal_title：Nucleic acids research

authors： Lau JC,Hanel ML,Wevrick R

更新日期：2004-06-24 00:00:00

abstract：:A frequently used method of comparing large RNA molecules employs the two-dimensional display of oligonucleotides generated through the action of specific RNases (oligonucleotide mapping, fingerprinting). Using computer simulations and simple analytic expressions the number of large RNase T1-resistant oligonucleotides...

journal_title：Nucleic acids research

authors： Aaronson RP,Young JF,Palese P

更新日期：1982-01-11 00:00:00