Transactivation and growth suppression by the gut-enriched Krüppel-like factor (Krüppel-like factor 4) are dependent on acidic amino acid residues and protein-protein interaction.

abstract：:Type I CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated) systems exist in bacterial and archaeal organisms and provide immunity against foreign DNA. The Cas protein content of the DNA interference complexes (termed Cascade) varies between different CRISPR-Cas subtypes. A minima...

journal_title：Nucleic acids research

authors： Dwarakanath S,Brenzinger S,Gleditzsch D,Plagens A,Klingl A,Thormann K,Randau L

更新日期：2015-10-15 00:00:00

abstract：:enod40 is a plant gene that participates in the regulation of symbiotic interaction between leguminous plants and bacteria or fungi. Furthermore, it has been suggested to play a general role in non-symbiotic plant development. Although enod40 seems to have multiple functions, being present in many land plants, the mol...

journal_title：Nucleic acids research

authors： Gultyaev AP,Roussis A

更新日期：2007-01-01 00:00:00

abstract：:Giant viruses have extraordinarily large dsDNA genomes, and exceptionally, they encode various components of the translation apparatus, including tRNAs, aminoacyl-tRNA synthetases and translation factors. Here, we focused on the elongation factor 1 (EF1) family of viral translational GTPases (trGTPases), using computa...

journal_title：Nucleic acids research

authors： Zinoviev A,Kuroha K,Pestova TV,Hellen CUT

更新日期：2019-06-20 00:00:00

abstract：:Rap1 is an essential DNA-binding factor from the yeast Saccharomyces cerevisiae involved in transcription and telomere maintenance. Its binding to DNA targets Rap1 at particular loci, and may optimize its ability to form functional macromolecular assemblies. It is a modular protein, rich in large potentially unfolded ...

journal_title：Nucleic acids research

authors： Matot B,Le Bihan YV,Lescasse R,Pérez J,Miron S,David G,Castaing B,Weber P,Raynal B,Zinn-Justin S,Gasparini S,Le Du MH

更新日期：2012-04-01 00:00:00

abstract：:RNase P is primarily responsible for the 5΄ maturation of transfer RNAs (tRNAs) in all domains of life. Archaeal RNase P is a ribonucleoprotein made up of one catalytic RNA and five protein cofactors including L7Ae, which is known to bind the kink-turn (K-turn), an RNA structural element that causes axial bending. How...

journal_title：Nucleic acids research

authors： Lai LB,Tanimoto A,Lai SM,Chen WY,Marathe IA,Westhof E,Wysocki VH,Gopalan V

更新日期：2017-07-07 00:00:00

abstract：:Linkage analysis is a successful procedure to associate diseases with specific genomic regions. These regions are often large, containing hundreds of genes, which make experimental methods employed to identify the disease gene arduous and expensive. We present two methods to prioritize candidates for further experimen...

journal_title：Nucleic acids research

authors： George RA,Liu JY,Feng LL,Bryson-Richardson RJ,Fatkin D,Wouters MA

更新日期：2006-01-01 00:00:00

abstract：:The Dnd (DNA degradation) phenotype, reflecting a novel DNA modification by sulfur in Streptomyces lividans 1326, was strongly aggravated when one (dndB) of the five genes (dndABCDE) controlling it was mutated. Electrophoretic banding patterns of a plasmid (pHZ209), reflecting DNA degradation, displayed a clear change...

journal_title：Nucleic acids research

authors： Liang J,Wang Z,He X,Li J,Zhou X,Deng Z

更新日期：2007-01-01 00:00:00

abstract：UNLABELLED:Partial cleavage of DNA with the restriction endonuclease NotI (5'...GC/GGCCGC...3') is an important technique for genomic mapping. However, partial genomic cleavage with this enzyme is impaired by the agarose matrix in which the DNA must be suspended. To solve this problem we have purified the blocking meth...

journal_title：Nucleic acids research

authors： Hanish J,McClelland M

更新日期：1990-06-11 00:00:00

abstract：:Genomes undergo changes in organization as a result of gene duplications, chromosomal rearrangements and local mutations, among other mechanisms. In contrast to prokaryotes, in which genes of a common function are often organized in operons and reside contiguously along the genome, most eukaryotes show much weaker clu...

journal_title：Nucleic acids research

authors： Thévenin A,Ein-Dor L,Ozery-Flato M,Shamir R

更新日期：2014-09-01 00:00:00

abstract：:A 170bp long BamHI-Sau3A DNA fragment from the actIII-actI intergenic region of the actinorhodin (Act) biosynthetic gene cluster of Streptomyces coelicolor A3(2) contains two promoters directing transcription in a divergent manner. One of them, the actIII promoter, is responsible for the transcription of the actIII ge...

journal_title：Nucleic acids research

authors： Parro V,Hopwood DA,Malpartida F,Mellado RP

更新日期：1991-05-25 00:00:00

abstract：:A new method of representing DNA sequences has been devised which is termed stave projection. Compared with other formats for showing the base sequences of DNA, this method greatly enhances the ease of visual analysis of the sequences of bases and it is also in a machine readable form. Using this method it is possible...

journal_title：Nucleic acids research

authors： Cowin JE,Jellis CH,Rickwood D

更新日期：1986-01-10 00:00:00

abstract：:The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenoty...

journal_title：Nucleic acids research

authors： Triman KL

更新日期：1996-01-01 00:00:00

abstract：:Intragenomic and intergenomic comparisons of upstream nucleotide sequences of archaeal genes were performed with the goal of predicting transcription regulatory sites (operators) and identifying likely regulons. Learning sets for the detection of regulatory sites were constructed using the available experimental data ...

journal_title：Nucleic acids research

authors： Gelfand MS,Koonin EV,Mironov AA

更新日期：2000-02-01 00:00:00

abstract：:DNA breaks occur during many processes in mammalian cells, including recombination, repair, mutagenesis and apoptosis. Here we report a simple and rapid method for assaying DNA breaks and identifying DNA breaksites. Breaksites are first tagged and amplified by ligation-mediated PCR (LM-PCR), using nested PCR primers t...

journal_title：Nucleic acids research

authors： Kong Q,Maizels N

更新日期：2001-03-15 00:00:00

abstract：:The petunia rbcS gene SSU301 was introduced into tobacco using Agrobacterium tumefaciens-mediated transformation. The time at which rbcS expression was maximal after transfer of the tobacco plants to the greenhouse was determined. The expression level of the SSU301 gene varied up to 9 fold between individual tobacco p...

journal_title：Nucleic acids research

authors： Dean C,Jones J,Favreau M,Dunsmuir P,Bedbrook J

更新日期：1988-10-11 00:00:00

abstract：:The sequence of a DNA fragment about 1 Kbp long located at the 3' boundary of the chicken alpha globin gene domain, including the 3'-side matrix attachment point and the site of transcription termination, was determined. It contains a repetitive DNA element and the AT-rich (easily denaturable) DNA segment conserved at...

journal_title：Nucleic acids research

authors： Farache G,Razin SV,Targa FR,Scherrer K

更新日期：1990-02-11 00:00:00

abstract：:Loss-of-function in melanocortin 1 receptor (MC1R), a GS protein-coupled receptor that regulates signal transduction through cAMP and protein kinase A (PKA) in melanocytes, is a major inherited melanoma risk factor. Herein, we report a novel cAMP-mediated response for sensing and responding to UV-induced DNA damage re...

journal_title：Nucleic acids research

authors： Jarrett SG,Wolf Horrell EM,D'Orazio JA

更新日期：2016-12-15 00:00:00

abstract：:Alu insertions have contributed to >11% of the human genome and ∼30-35 Alu subfamilies remain actively mobile, yet the characterization of polymorphic Alu insertions from short-read data remains a challenge. We build on existing computational methods to combine Alu detection and de novo assembly of WGS data as a means...

journal_title：Nucleic acids research

authors： Wildschutte JH,Baron A,Diroff NM,Kidd JM

更新日期：2015-12-02 00:00:00

abstract：:Tetrahymena thermophila mitochondrial DNA is a linear molecule with two tRNAs, large subunit beta (LSU beta) rRNA (21S rRNA) and LSU alpha rRNA (5.8S-like RNA) encoded near each terminus. The DNA sequence of approximately 550 bp of this region was determined in six species of Tetrahymena. In three species the LSU beta...

journal_title：Nucleic acids research

authors： Morin GB,Cech TR

更新日期：1988-01-11 00:00:00

abstract：:The nucleotide sequence of the Anacystis nidulans 23S rRNA gene, including the 5'- and 3'-flanking regions has been determined. The gene is 2876 nucleotides long and shows higher primary sequence homology to the 23S rRNAs of plastids (84.5%) than to that of E. coli (79%). The predicted rRNA transcript also shares many...

journal_title：Nucleic acids research

authors： Douglas SE,Doolittle WF

更新日期：1984-04-11 00:00:00

abstract：:The homeobox-containing gene Hex is expressed in several cell types, including thyroid follicular cells, in which it regulates the transcription of tissue- specific genes. In this study the regulation of Hex promoter activity was investigated. Using co- transfection experiments, we demonstrated that the transcriptiona...

journal_title：Nucleic acids research

authors： Puppin C,D'Elia AV,Pellizzari L,Russo D,Arturi F,Presta I,Filetti S,Bogue CW,Denson LA,Damante G

更新日期：2003-04-01 00:00:00

abstract：:The transcription factor HIV-TF1, which binds to a region about 60 bp upstream from the enhancer of the human immunodeficiency virus-1 (HIV-1), was purified from human B cells. HIV-TF1 had a molecular weight of 39,000. Binding of HIV-TF1 to the HIV long terminal repeat (LTR) activated transcription from the HIV promot...

journal_title：Nucleic acids research

authors： Maekawa T,Sudo T,Kurimoto M,Ishii S

更新日期：1991-09-11 00:00:00

abstract：:We have investigated the attachment of the DNA to the nuclear matrix during the division cycle of the plasmodial slime mold Physarum polycephalum. The DNA of plasmodia was pulse labelled at different times during the S phase and the label distribution was studied by graded DNase digestion of the matrix-DNA complexes p...

journal_title：Nucleic acids research

authors： Aelen JM,Opstelten RJ,Wanka F

更新日期：1983-02-25 00:00:00

abstract：:PDB-REPRDB is a database of representative protein chains from the Protein Data Bank (PDB). Started at the Real World Computing Partnership (RWCP) in August 1997, it developed to the present system of PDB-REPRDB. In April 2001, the system was moved to the Computational Biology Research Center (CBRC), National Institut...

journal_title：Nucleic acids research

authors： Noguchi T,Akiyama Y

更新日期：2003-01-01 00:00:00

abstract：:Chemically modified siRNAs are expected to have resistance toward nuclease degradation and good thermal stability in duplex formation for in vivo applications. We have recently found that 2'-OMe-4'-thioRNA, a hybrid chemical modification based on 2'-OMeRNA and 4'-thioRNA, has high hybridization affinity for complement...

journal_title：Nucleic acids research

authors： Takahashi M,Nagai C,Hatakeyama H,Minakawa N,Harashima H,Matsuda A

更新日期：2012-07-01 00:00:00

abstract：:The gene for the small subunit of human calcium-activated neutral protease was isolated and sequenced. It is 11 kb long and comprises 11 exons. No TATA or CAT box was found upstream of the possible transcription initiation sites, but there are three so-called G-C box sequences and one G-C box-like sequence, which are ...

journal_title：Nucleic acids research

authors： Miyake S,Emori Y,Suzuki K

更新日期：1986-11-25 00:00:00

abstract：:REBASE is a comprehensive database of information about restriction enzymes and their associated methylases, including their recognition and cleavage sites and their commercial availability. Also included is a listing of homing endonucleases. Information from REBASE is available via monthly electronic mailings as well...

journal_title：Nucleic acids research

authors： Roberts RJ,Macelis D

更新日期：1998-01-01 00:00:00

abstract：:Mutations in the RecQ DNA helicase gene BLM give rise to Bloom's syndrome, which is a rare autosomal recessive disorder characterized by genetic instability and cancer predisposition. BLM helicase is highly active in binding and unwinding G-quadruplexes (G4s), which are physiological targets for BLM, as revealed by ge...

journal_title：Nucleic acids research

authors： Wu WQ,Hou XM,Li M,Dou SX,Xi XG

更新日期：2015-05-19 00:00:00

abstract：:For characterizing in vivo functions of a mammalian protein, it is informative to obtain conditional mutations and apply them to the mouse genetic system. However, the isolation of conditional mutations has been quite difficult in cultured cells. We report here that functional expression of a heterologous mammalian ge...

journal_title：Nucleic acids research

authors： Adachi N,Ikeda H,Kikuchi A

更新日期：1994-10-11 00:00:00

abstract：:The E. coli single-stranded binding protein (SSB) has been demonstrated in vitro to be involved in a number of replicative, DNA renaturation, and protective functions. It was shown previously that SSB can interact with exonuclease I to stimulate the hydrolysis of single-stranded DNA. We demonstrate here that E. coli S...

journal_title：Nucleic acids research

authors： Sandigursky M,Franklin WA

更新日期：1994-01-25 00:00:00