Abstract：
:Ornithine decarboxylase (ODC) expression is important for proliferation and is elevated in many tumor cells. We previously showed that Sp1 is a major positive regulator of ODC transcription. In this paper we have investigated transcriptional regulation of rat ODC by the closely related factor Sp3. While over-expression of Sp1 caused a dramatic activation of the ODC promoter, over-expression of Sp3 caused little or no activation in either Drosophila SL2 cells (lacking endogenous Sp1 or Sp3) or in H35 rat hepatoma cells. Furthermore, co-transfection studies demonstrated that Sp3 abolished trans -activation of the ODC promoter by Sp1. DNase I footprint studies and electrophoretic mobility shift assays demonstrated that both recombinant Sp1 and Sp3 bind specifically to several sites within the ODC promoter also protected by nuclear extracts, including overlapping GC and CT motifs located between -116 and -104. This CT element is a site of negative ODC regulation. Mutation of either element reduced binding, but mutation of both sites was required to eliminate binding of either Sp1 or Sp3. These results demonstrate that ODC is positively regulated by Sp1 and negatively regulated by Sp3, suggesting that the ratio of these transcription factors may be an important determinant of ODC expression during development or transformation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Kumar AP,Butler APdoi
10.1093/nar/25.10.2012subject
Has Abstractpub_date
1997-05-15 00:00:00pages
2012-9issue
10eissn
0305-1048issn
1362-4962pii
gka334journal_volume
25pub_type
杂志文章abstract：:The cnjC gene from the protozoan Tetrahymena thermophila was completely sequenced. The deduced gene product was found to have significant sequence similarity to the yeast and prokaryotic RNA polymerase subunits involved with subunit assembly. Since cnjC is active only during the sexual stage (conjugation) of Tetrahyme...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.10.2953
更新日期：1990-05-25 00:00:00
abstract：:Leishmania species are protozoan parasites whose remarkably plastic genome limits the establishment of effective genetic manipulation and leishmaniasis treatment. The strategies used by Leishmania to maintain its genome while allowing variability are not fully understood. Here, we used DiCre-mediated conditional gene ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1017
更新日期：2018-12-14 00:00:00
abstract：:The Gibbs Motif Sampler is a software package for locating common elements in collections of biopolymer sequences. In this paper we describe a new variation of the Gibbs Motif Sampler, the Gibbs Recursive Sampler, which has been developed specifically for locating multiple transcription factor binding sites for multip...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg608
更新日期：2003-07-01 00:00:00
abstract：:The pattern of sites for cleavage mediated by topoisomerase II was determined in 830 kb of cloned DNA from the Drosophila X chromosome, with the objectives of comparing it with mapped structural and functional landmarks and examining if the correlations with such landmarks reported in individual loci can be generalize...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.11.2041
更新日期：1997-06-01 00:00:00
abstract：:In plants, small interfering RNAs (siRNAs) can trigger a silencing signal that may spread within a tissue to adjacent cells or even systemically to other organs. Movement of the signal is initially limited to a few cells, but in some cases the signal can be amplified and travel over larger distances. How far silencing...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1240
更新日期：2011-04-01 00:00:00
abstract：:2'-Deoxyoligonucleotides with 5-fluorocytosine residues incorporated at specific positions of the nucleotide sequence are tools of great potential in the study of the catalytic mechanism by which DNA cytosine methyltransferases methylate the 5-position of DNA cytosine residues in specific sequence contexts. Chemical s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.10.2421
更新日期：1992-05-25 00:00:00
abstract：:A peptide initiation factor purified from rat liver and promoting the binding of initiator tRNA and model initiators to 40S and 80S ribosome at an acid pH liberates methionine and N-acetylmethionine from Trna Met f at neutral reaction. Phenylalanyl-tRNA, N-acetylphenylalanyl-tRNA and methionyl-tRNA Met m are not hydro...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.11.2119
更新日期：1975-11-01 00:00:00
abstract：:To investigate the mechanisms by which oligonucleotides hybridize to target molecules, the binding of two oligodeoxynucleotide probes to RNA targets was measured over a broad range of temperatures. Mutations were then scanned across each DNA/RNA hybrid to map, at single base resolution, sequences important for hybridi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn134
更新日期：2008-05-01 00:00:00
abstract：:A growing number of whole genome sequencing projects, in combination with development of phylogenetic methods for reconstructing gene evolution, have provided us with a window into genomes that existed millions, and even billions, of years ago. Ancestral Genomes (http://ancestralgenomes.org) is a resource for comprehe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1009
更新日期：2019-01-08 00:00:00
abstract：:A circular denaturation and restriction map of mitochondrial DNA from Neurospora crassa is presented. The map shows the position of all twelve fragments produced by restriction endonuclease Eco R I and the position of the largest Hin III fragment along the previously established map of AT-rich sequences. The two wild ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.11.3101
更新日期：1976-11-01 00:00:00
abstract：:We describe a new, broadly applicable methodology for screening in parallel interactions of RNA-binding proteins (RBPs) with large numbers of microRNA (miRNA) precursors and for determining their affinities in native form in the presence of cellular factors. The assays aim at identifying pre-miRNAs that are potentiall...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1197
更新日期：2013-02-01 00:00:00
abstract：:The cDNA-chip technology is a highly versatile tool for the comprehensive analysis of gene expression at the transcript level. Although it has been applied successfully in expression profiling projects, there is an ongoing dispute concerning the quality of such expression data. The latter critically depends on the spe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gng001
更新日期：2003-01-15 00:00:00
abstract：:A 200 bp sequence including a stretch of 54 base pairs of alternating guanosine and adenosine nucleotide residues [(dG-dA)27.(dT-dC)27] was cloned in the simian virus 40 (SV40) genome between the KpnI and HpaII sites. This sequence was discovered earlier as part of a region limiting the amplification of sequences adja...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.16.8077
更新日期：1988-08-25 00:00:00
abstract：:Various antitumor drugs stabilize DNA topoisomerase II-DNA transient covalent complexes. The complexes distribution along pBR322 DNA was shown previously to depend upon the nature of the drug (Tewey et al. (1984) Science 226, 466-468). The position in pBR322 of DNA cleavage by calf DNA topoisomerase II for 115 such si...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.11.2861
更新日期：1991-06-11 00:00:00
abstract：:In an RNase III-deficient strain of E. coli 23S pre-rRNA accumulates unprocessed in 50S ribosomes and in polysomes. These ribosomes provide a substrate for the analysis of rRNA maturation in vitro. S1 nuclease protection analysis of the products obtained in in vitro processing reactions demonstrates that 23S rRNA proc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.14.5041
更新日期：1985-07-25 00:00:00
abstract：:A common aim in ChIP-seq experiments is to identify changes in protein binding patterns between conditions, i.e. differential binding. A number of peak- and window-based strategies have been developed to detect differential binding when the regions of interest are not known in advance. However, careful consideration o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku351
更新日期：2014-06-01 00:00:00
abstract：:The principal early promoter of human papillomaviruses (HPVs), designated P97 in the case of HPV-16, contains four characteristically aligned cis-responsive elements, namely one binding site for Sp1, two for the viral E2 proteins, and the TATA box. The Sp1 binding site is needed to mediate activation of P97 by the rem...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.2.251
更新日期：1992-01-25 00:00:00
abstract：:The crystal structure of the DNA dodecamer duplex CATGGGCCCATG lies on a structural continuum along the transition between A- and B-DNA. The dodecamer possesses the normal vector plot and inclination values typical of B-DNA, but has the crystal packing, helical twist, groove width, sugar pucker, slide and x-displaceme...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkf515
更新日期：2002-09-15 00:00:00
abstract：:The 3' terminal nucleotide sequence of two clones containing DNA complementary to mu chain mRNA of IgM-secreting cells has been determined. The sequence shows a termination codon (UGA) adjacent to the terminal tyrosine codon for the secreted protein and a 3' non-coding region of at least 106 bases. The primary transla...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：
更新日期：1980-02-25 00:00:00
abstract：:The structures of the termini and their flanking regions of two human KpnI family members were investigated. The two differed in length, but the starting sequence at one terminal (defined as the 5' terminal) was found to be common to both members. The Alu family sequence was found in the 5' flanking regions. The KpnI ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.19.6837
更新日期：1983-10-11 00:00:00
abstract：:Sequential chromatin immunoprecipitation (SeqChIP) is a procedure in which formaldehyde-crosslinked, protein-DNA complexes from living cells are subjected to two sequential immunoprecipitations with antibodies of different specificity. SeqChIP has been used to address, in a qualitative manner, whether two proteins can...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh148
更新日期：2004-11-01 00:00:00
abstract：:Nucleotide excision repair is initiated by two different damage recognition subpathways, global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, XPC detects DNA lesions and recruits TFIIH via interaction with the pleckstrin homology (PH) domain of TFIIH subunit p62. In TCR, an elongating form of RNA...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx970
更新日期：2017-12-15 00:00:00
abstract：:U4 RNA from chicken, rat and man was examined for nucleotide sequence and secondary structure. Three molecular species, U4A, U4B and U4C were detected in the three animal species. U4A is 146 nucleotide long and U4B RNA only lacks the 3' terminal G. four nucleotides are missing at the 3'-end of U4C RNA which, in additi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.12.2699
更新日期：1981-06-25 00:00:00
abstract：:The human 7SL RNA component of the signal recognition particle can be separated into four major conformers by nondenaturing polyacrylamide gel electrophoresis. We have investigated what sequences give 7SL RNA the property to exist in different conformations. The human 7SL gene 7L30.1 was mutagenized using the random l...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.11.4639
更新日期：1986-06-11 00:00:00
abstract：:The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at approximately 20% total cell protein (tcp) and the Bordetella pertussis antigen pertactin at greater than 30% tcp. These levels a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.11.2889
更新日期：1991-06-11 00:00:00
abstract：:Studies of bacterial chromosomes and plasmids indicate that their replication initiator proteins bind to origins of replication at many double-stranded sites and also at AT-rich regions where single-stranded DNA is exposed during origin opening. Single-strand binding apparently promotes origin opening by stabilizing a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa826
更新日期：2020-11-04 00:00:00
abstract：:We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the human papillomavirus E6...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn903
更新日期：2009-01-01 00:00:00
abstract：:We attempted to use the polymerase chain reaction (PCR) to monitor in vitro recombination in a plasmid containing directly repeated sequences. Some of the plasmid preparations which had not been exposed to recombination conditions were however found to behave in the PCR test as if they had undergone homologous recombi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.9.2423
更新日期：1991-05-11 00:00:00
abstract：:DNA topoisomerases and DNA site-specific recombinases are biologically important enzymes involved in a diverse set of cellular processes. We show that replacement of a phosphodiester linkage by a 5'-bridging phosphorothioate linkage creates an efficient suicide substrate for calf thymus topoisomerase I and lambda inte...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.15.2973
更新日期：1995-08-11 00:00:00
abstract：:Pairing of a given E3 ubiquitin ligase with different E2s allows synthesis of ubiquitin conjugates of different topologies. While this phenomenon contributes to functional diversity, it remains largely unknown how a single E3 ubiquitin ligase recognizes multiple E2s, and whether identical structural requirements deter...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr655
更新日期：2012-01-01 00:00:00
