Abstract：
:We attempted to use the polymerase chain reaction (PCR) to monitor in vitro recombination in a plasmid containing directly repeated sequences. Some of the plasmid preparations which had not been exposed to recombination conditions were however found to behave in the PCR test as if they had undergone homologous recombination. We show here that such false positives are attributable to a small degree of nicking and/or breaking of the DNA template. Presumably, such damage allows the formation of hybrid parental duplexes containing at least one truncated strand, the 3' end of which maps within the homology; extension of this 3' end by the polymerase then results in a linkage of sequences identical to that arising from homologous recombination.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Marton A,Delbecchi L,Bourgaux Pdoi
10.1093/nar/19.9.2423subject
Has Abstractpub_date
1991-05-11 00:00:00pages
2423-6issue
9eissn
0305-1048issn
1362-4962journal_volume
19pub_type
杂志文章abstract：:An updated list of DNA sequences cloned from the human genome over the past year (1991) is presented. Intended as a guide to clone availability, this list includes published reports of cDNA, genomic and synthetic probes comprising both gene and pseudogene sequences. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.suppl.2181
更新日期：1992-05-11 00:00:00
abstract：:Isogenic pluripotent stem cells are critical tools for studying human neurological diseases by allowing one to study the effects of a mutation in a fixed genetic background. Of particular interest are the spectrum of autism disorders, some of which are monogenic such as Timothy syndrome (TS); others are multigenic suc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv164
更新日期：2015-05-26 00:00:00
abstract：:Despite the increasing wealth of available data, the structure of cancer transcriptional space remains largely unknown. Analysis of this space would provide novel insights into the complexity of cancer, assess relative implications in complex biological processes and responses, evaluate the effectiveness of cancer mod...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr533
更新日期：2011-10-01 00:00:00
abstract：:Many interacting proteins regulate and/or assist the activities of RAD51, a recombinase which plays a critical role in both DNA repair and meiotic recombination. Yeast two-hybrid screening of a human testis cDNA library revealed a new protein, RAD51AP2 (RAD51 Associated Protein 2), that interacts strongly with RAD51. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl665
更新日期：2006-01-01 00:00:00
abstract：:The cyanobacteirum Synechocystis sp. strain PCC 6803 possesses nine species of the sigma (sigma)-factor gene for RNA polymerase (RNAP). Here, we identify and characterize the novel-type promoter recognized by a group 3 sigma-factor, SigF. SigF autoregulates its own transcription and recognizes the promoter of pilA1 th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn453
更新日期：2008-09-01 00:00:00
abstract：:Integrating DNA delivery systems hold promise for many applications including treatment of diseases; however, targeted integration is needed for improved safety. The piggyBac (PB) transposon system is a highly active non-viral gene delivery system capable of integrating defined DNA segments into host chromosomes witho...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx572
更新日期：2017-08-21 00:00:00
abstract：:The yeast retrotransposon Ty encodes proteins that assemble into virus-like particles (Ty-VLPs) which can be readily purified. We have recently shown that expression of the pl protein encoded by the TYA gene of Ty is sufficient for particle formation. In this paper we show that when a heterologous coding sequence, hum...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.18.7571
更新日期：1987-09-25 00:00:00
abstract：:Functional crosstalk between histone modifications and chromatin remodeling has emerged as a key regulatory mode of transcriptional control during cell fate decisions, but the underlying mechanisms are not fully understood. Here we discover an HRP2-DPF3a-BAF epigenetic pathway that coordinates methylated histone H3 ly...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa441
更新日期：2020-07-09 00:00:00
abstract：:Cisplatin analogues were synthesised that consisted of platinum(II) diamine complexes tethered via a polymethylene chain ( n = 3, 5, 8 and 10) to a phenanthridinium cation. Both chloro and iodo leaving groups were examined. DNA adduct formation was quantitatively analysed using a linear amplification system with the p...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.17.3933
更新日期：1998-09-01 00:00:00
abstract：:Recently, prokaryotic riboswitches have been identified that regulate transcription in response to change of the concentration of secondary messengers. The ZMP (5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR))-sensing riboswitch from Thermosinus carboxydivorans is a transcriptional ON-switch that is involved in ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa427
更新日期：2020-07-09 00:00:00
abstract：:The human 5S rRNA genes are found in clusters of tandem repeated units. We have cloned and partially characterized six restriction fragments from two clusters of 2.3 kb and 1.6 kb repeats, respectively. Four fragments from the cluster of 2.3 kb repeats contain a 5S rRNA gene and one fragment contains a gene variant wi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.15.4147
更新日期：1991-08-11 00:00:00
abstract：:Mechanisms that preserve genome integrity are highly important during the normal life cycle of human cells. Loss of genome protective mechanisms can lead to the development of diseases such as cancer. Checkpoint kinases function in the cellular surveillance pathways that help cells to cope with DNA damage. Importantly...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkr697
更新日期：2012-01-01 00:00:00
abstract：:Topoisomerases are ubiquitous proteins found in all three domains of life. They change the topology of DNA via transient breaks on either one or two of the DNA strands to allow passage of another single or double DNA strand through the break. Topoisomerases are classified into two types: type I enzymes cleave one DNA ...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkn1009
更新日期：2009-02-01 00:00:00
abstract：:The Mouse Genome Database (MGD) focuses on the integration of mapping, homology, polymorphism and molecular data about the laboratory mouse. Detailed descriptions of genes including their chromosomal location, gene function, disease associations, mutant phenotypes, molecular polymorphisms and links to representative s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.1.95
更新日期：1999-01-01 00:00:00
abstract：UNLABELLED:The PubMed® database of biomedical citations allows the retrieval of scientific articles studying the function of chemicals in biology and medicine. Mining millions of available citations to search reported associations between chemicals and topics of interest would require substantial human time. We have im...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku432
更新日期：2014-07-01 00:00:00
abstract：:'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made with 6 to 8 base substitutions in the center of the primers. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.16.3745
更新日期：1993-08-11 00:00:00
abstract：:DNA topoisomerases and DNA site-specific recombinases are biologically important enzymes involved in a diverse set of cellular processes. We show that replacement of a phosphodiester linkage by a 5'-bridging phosphorothioate linkage creates an efficient suicide substrate for calf thymus topoisomerase I and lambda inte...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.15.2973
更新日期：1995-08-11 00:00:00
abstract：:The long terminal repeat (LTR) of the proviral human immunodeficiency virus (HIV)-1 genome is integral to virus transcription and host cell infection. The guanine-rich U3 region within the LTR promoter, previously shown to form G-quadruplex structures, represents an attractive target to inhibit HIV transcription and r...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw432
更新日期：2016-07-27 00:00:00
abstract：:The entire nucleotide sequence of the Ad. 2 EcoRI D fragment has been determined using the Maxam and Gilbert method. This sequence of 2678 bp contains informations relative to late mRNAs ending at position 78 and for which an AATAAA sequence corresponding to their 3' ends is found at residue number 833. Position of th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.10.2173
更新日期：1980-05-24 00:00:00
abstract：:In this study we describe a novel method to investigate the RNA-RNA interactions between a small RNA and its target that we termed 'RNA walk'. The method is based on UV-induced AMT cross-linking in vivo followed by affinity selection of the hybrid molecules and mapping the intermolecular adducts by RT-PCR or real-time...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp872
更新日期：2010-01-01 00:00:00
abstract：:During protein synthesis, the nascent peptide chain traverses the peptide exit tunnel of the ribosome. We monitor the co-translational movement of the nascent peptide using a fluorescent probe attached to the N-terminus of the nascent chain. Due to fluorophore quenching, the time-dependent fluorescence signal emitted ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky740
更新日期：2018-12-14 00:00:00
abstract：:This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A) x n poly(U) (n = 1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered struc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.18.4283
更新日期：1980-09-25 00:00:00
abstract：:We have isolated and characterized a S. pombe promoter using a functional heterologous gene product assay. Random S. pombe genomic fragments were cloned upstream from the promoterless 'lacZ gene and tested in vivo for their efficiency to promote expression of the beta-galactosidase protein in the fission yeast. An eff...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.17.8603
更新日期：1988-09-12 00:00:00
abstract：:Targeted differentiation of human induced pluripotent stem cells (hiPSCs) using only chemicals would have value-added clinical potential in the regeneration of complex cell types including cardiomyocytes. Despite the availability of several chemical inhibitors targeting proteins involved in signaling pathways, no bioa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx693
更新日期：2017-09-19 00:00:00
abstract：:DNA supercoiling is an important regulator of gene activity. The transmission of transcription-generated supercoiling wave along a DNA helix provides a way for a gene being transcribed to communicate with and regulate its neighboring genes. Currently, the dynamic behavior of supercoiling transmission remains unclear o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky534
更新日期：2018-08-21 00:00:00
abstract：:Recent rapid advances in next generation RNA sequencing (RNA-Seq)-based provide researchers with unprecedentedly large data sets and open new perspectives in transcriptomics. Furthermore, RNA-Seq-based transcript profiling can be applied to non-model and newly discovered organisms because it does not require a predefi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks540
更新日期：2012-07-01 00:00:00
abstract：:Transcriptional regulatory networks specify regulatory proteins controlling the context-specific expression levels of genes. Inference of genome-wide regulatory networks is central to understanding gene regulation, but remains an open challenge. Expression-based network inference is among the most popular methods to i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw963
更新日期：2017-02-28 00:00:00
abstract：:Three different types of repetitive Eco RI fragments, which comigrate within a visible band of approximately 500 bp at gel electrophoresis of Xenopus laevis DNA Eco RI digests have been cloned and sequenced. These sequences are designated as Repetitive Eco RI Monomers: REM 1, REM 2 and REM 3. The sequences contain dir...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.12.4921
更新日期：1984-06-25 00:00:00
abstract：:We have modeled local DNA sequence parameters to search for DNA architectural motifs involved in transcription regulation and promotion within the Xenopus laevis ribosomal gene promoter and the intergenic spacer (IGS) sequences. The IGS was found to be shaped into distinct topological domains. First, intrinsic bends s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.18.3433
更新日期：2000-09-15 00:00:00
abstract：:OligoArrayDb is a comprehensive database containing pangenomic oligonucleotide microarray probe sets designed for most of the sequenced genomes that are not covered by commercial catalog arrays. The availability of probe sequences, associated with custom microarray fabrication services offered by many companies and co...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn761
更新日期：2009-01-01 00:00:00
