Comparative analysis of non-autonomous effects of tasiRNAs and miRNAs in Arabidopsis thaliana.


:In plants, small interfering RNAs (siRNAs) can trigger a silencing signal that may spread within a tissue to adjacent cells or even systemically to other organs. Movement of the signal is initially limited to a few cells, but in some cases the signal can be amplified and travel over larger distances. How far silencing initiated by other classes of plant small RNAs (sRNAs) than siRNAs can extend has been less clear. Using a system based on the silencing of the CH42 gene, we have tracked the mobility of silencing signals initiated in phloem companion cells by artificial microRNAs (miRNA) and trans-acting siRNA (tasiRNA) that have the same primary sequence. In this system, both the ta-siRNA and the miRNA act at a distance. Non-autonomous effects of the miRNA can be triggered by several different miRNA precursors deployed as backbones. While the tasiRNA also acts non-autonomously, it has a much greater range than the miRNA or hairpin-derived siRNAs directed against CH42, indicating that biogenesis can determine the non-autonomous effects of sRNAs. In agreement with this hypothesis, the silencing signals initiated by different sRNAs differ in their genetic requirements.


Nucleic Acids Res


Nucleic acids research


de Felippes FF,Ott F,Weigel D




Has Abstract


2011-04-01 00:00:00














  • General method of rapid Smith/Birnstiel mapping adds for gap closure in shotgun microbial genome sequencing projects: application to Pseudomonas putida KT2440.

    abstract::A physical mapping strategy has been developed to verify and accelerate the assembly and gap closure phase of a microbial genome shotgun-sequencing project. The protocol was worked out during the ongoing Pseudomonas putida KT2440 genome project. A macro-restriction map was constructed by linking probe hybridisation of...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Weinel C,Tümmler B,Hilbert H,Nelson KE,Kiewitz C

    更新日期:2001-11-15 00:00:00

  • Structure of the 70S ribosome from human pathogen Staphylococcus aureus.

    abstract::Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact translation specificity and its regulation. Although the importance of such features may be less obvious within more closely rela...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Khusainov I,Vicens Q,Bochler A,Grosse F,Myasnikov A,Ménétret JF,Chicher J,Marzi S,Romby P,Yusupova G,Yusupov M,Hashem Y

    更新日期:2016-12-01 00:00:00

  • Polymerase chain reaction mapping of yeast GAL7 mRNA polyadenylation sites demonstrates that 3' end processing in vitro faithfully reproduces the 3' ends observed in vivo.

    abstract::In general, synthetic RNA transcripts corresponding to the 3' ends of Saccharomyces cerevisiae genes appear to be accurately cleaved and polyadenylated in vitro under appropriate conditions in yeast cell extracts. Initially, however, the endpoints observed in vitro for the GAL7 gene failed to correlate adequately with...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sadhale PP,Sapolsky R,Davis RW,Butler JS,Platt T

    更新日期:1991-07-11 00:00:00

  • Structural insights into dynamics of RecU-HJ complex formation elucidates key role of NTR and stalk region toward formation of reactive state.

    abstract::Holliday junction (HJ) resolving enzyme RecU is involved in DNA repair and recombination. We have determined the crystal structure of inactive mutant (D88N) of RecU from Bacillus subtilis in complex with a 12 base palindromic DNA fragment at a resolution of 3.2 Å. This structure shows the stalk region and the essentia...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Khavnekar S,Dantu SC,Sedelnikova S,Ayora S,Rafferty J,Kale A

    更新日期:2017-01-25 00:00:00

  • Autoregulation of MBNL1 function by exon 1 exclusion from MBNL1 transcript.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Konieczny P,Stepniak-Konieczna E,Taylor K,Sznajder LJ,Sobczak K

    更新日期:2017-02-28 00:00:00

  • Examining the contribution of a dA+dT element to the conformation of Escherichia coli integration host factor-DNA complexes.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Hales LM,Gumport RI,Gardner JF

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Waddell J,Wang XM,Wu M

    更新日期:1984-05-11 00:00:00

  • Activation of the Bcl-2 promoter by nerve growth factor is mediated by the p42/p44 MAPK cascade.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


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  • Identify bilayer modules via pseudo-3D clustering: applications to miRNA-gene bilayer networks.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Xu Y,Guo M,Liu X,Wang C,Liu Y,Liu G

    更新日期:2016-11-16 00:00:00

  • Sequence-specific interactions of the tight-binding I12-X86 lac repressor with non-operator DNA.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schmitz A,Galas DJ

    更新日期:1980-02-11 00:00:00

  • Highly efficient preparation of single-stranded DNA rings by T4 ligase at abnormally low Mg(II) concentration.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: An R,Li Q,Fan Y,Li J,Pan X,Komiyama M,Liang X

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Nguyen NTT,Vincens P,Roest Crollius H,Louis A

    更新日期:2018-01-04 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Hsu CH,Wang AH

    更新日期:2011-08-01 00:00:00

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    pub_type: 杂志文章


    authors: Dutreix M,Bäckman A,Célérier J,Bagdasarian MM,Sommer S,Bailone A,Devoret R,Bagdasarian M

    更新日期:1988-11-25 00:00:00

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    pub_type: 杂志文章


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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Bramlage B,Luzi E,Eckstein F

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    pub_type: 杂志文章


    authors: Berberof M,Vanhamme L,Alexandre S,Lips S,Tebabi P,Pays E

    更新日期:2000-01-15 00:00:00

  • The bacteriophage lambda O and P protein initiators promote the replication of single-stranded DNA.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kohwi-Shigematsu T,Kohwi Y

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chen CL,Liang D,Zhou H,Zhuo M,Chen YQ,Qu LH

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tsukahara T,Casciato C,Helfman DM

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Jay E,Bambara R,Padmanabhan R,Wu R

    更新日期:1974-03-01 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Dilek A,Belviranli ME,Dogrusoz U

    更新日期:2010-07-01 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Blanks R,McLaughlin LW

    更新日期:1988-11-11 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Copoiu L,Torres PHM,Ascher DB,Blundell TL,Malhotra S

    更新日期:2020-01-08 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Karahalil B,Girard PM,Boiteux S,Dizdaroglu M

    更新日期:1998-03-01 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Bibillo A,Lener D,Klarmann GJ,Le Grice SF

    更新日期:2005-01-12 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wolff J,Rabbani L,Gilsbach R,Richard G,Manke T,Backofen R,Grüning BA

    更新日期:2020-07-02 00:00:00