Abstract：
:Expression of the wild-type p53 tumor suppressor gene has been found to play an important role in the regulation of cellular proliferation and differentiation. In addition, in many transformed cells and primary tumors, the gene has undergone allelic deletions and mutant forms of the p53 gene are expressed at elevated levels. In defining transcriptional regulatory regions of the p53 gene, we have previously shown that both the human and murine p53 promoters contain a conserved consensus recognition sequence for the basic-helix-loop-helix (bHLH) containing family of DNA-binding proteins. In the murine p53 promoter this element is required for full promoter activity and contains the sequence CACGTG, a sequence identical to the recognition site for the bHLH containing transcription factors c-Myc, USF and TFE3. Here we examine the ability of one of these factors, USF, to bind to the p53 promoter. By assaying the binding activity of in vitro translated USF as well as factors present in nuclear extracts, we conclude that the transcription factor USF binds in a site-specific manner to a CACGTG motif within the murine p53 promoter and represents the major DNA-binding activity observed in nuclear extracts. Elevated levels of USF, generated upon transfection of a vector expressing USF, lead to enhanced activity of the p53 promoter. These findings indicate that USF may play a central role in regulating p53 expression.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Reisman D,Rotter Vdoi
10.1093/nar/21.2.345subject
Has Abstractpub_date
1993-01-25 00:00:00pages
345-50issue
2eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract：:The Genomes Online Database (GOLD) (https://gold.jgi.doe.gov) is a manually curated data management system that catalogs sequencing projects with associated metadata from around the world. In the current version of GOLD (v.6), all projects are organized based on a four level classification system in the form of a Stud...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw992
更新日期：2017-01-04 00:00:00
abstract：:We have carried out a survey of potential quadruplex structure sequences (PQSS), which occur in the immediate upstream region (500 bp) of human genes. By examining the number and distribution of these we have established that there is a clear link between them and the occurrence of the SP1-binding element 'GGGCGG', su...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn078
更新日期：2008-05-01 00:00:00
abstract：:The mitochondrial DNA of Neurospora crassa has a heterogeneous intramolecular base distribution. A contiguous piece, representing at least 30% of the total genome, has a G+C content that is 6% lower than the overall G+C content of the DNA. The genes for both ribosomal RNAs are contained in the remaining, relatively G+...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.1.129
更新日期：1977-01-01 00:00:00
abstract：:Three novel DNA-binding proteins with apparent molecular masses of 7, 10 and 30 kDa have been isolated from the hyperthermophilic methanogen Methanopyrus kandleri. The proteins were identified using a blot overlay assay that was modified to emulate the high ionic strength intracellular environment of M.kandleri protei...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.3.685
更新日期：2002-02-01 00:00:00
abstract：:In order to study the intragenic profiles of active transcription, we determined the relative levels of active RNA polymerase II present at the 3'- and 5'-ends of 261 yeast genes by run-on. The results obtained indicate that the 3'/5' run-on ratio varies among the genes studied by over 12 log(2) units. This ratio seem...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq215
更新日期：2010-08-01 00:00:00
abstract：:Human telomerase RNA (hTR) contains several guanine tracts at its 5'-end that can form a G4-quadruplex structure. Previous evidence suggests that a G4-quadruplex within this region disrupts the formation of an important structure within hTR known as the P1 helix, a critical element in defining the template boundary fo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1306
更新日期：2012-05-01 00:00:00
abstract：:We have created a database of comparatively derived group I intron secondary structure diagrams. This collection currently contains a broad sampling of phylogenetically and structurally similar and diverse structures from over 200 publicly available intron sequences. As more group I introns are sequenced and added to ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.17.3508
更新日期：1994-09-01 00:00:00
abstract：:The Zebrafish Information Network (ZFIN) (https://zfin.org/) is the database for the model organism, zebrafish (Danio rerio). ZFIN expertly curates, organizes, and provides a wide array of zebrafish genetic and genomic data, including genes, alleles, transgenic lines, gene expression, gene function, mutant phenotypes,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1010
更新日期：2021-01-08 00:00:00
abstract：:IL-2 gene expression in activated T-cells is initiated by chromatin remodeling at the IL-2 proximal promoter and conversion of a transcriptional repressor into a potent transcriptional activator. A purine-box regulator complex was purified from activated Jurkat T-cell nuclei based on sequence-specific DNA binding to t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm117
更新日期：2007-01-01 00:00:00
abstract：:Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (beta-S)triphosphates. Ini...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.21.8115
更新日期：1984-11-12 00:00:00
abstract：:Spermine-DNA complexes have been examined by small-angle and wide-angle X-ray scattering as well as by circular dichroism studies. Condensed complexes are building up below a critical ionic strength. We have found that at one and the same ionic strength condensed complexes having two different supramolecular structure...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/7.5.1297
更新日期：1979-11-10 00:00:00
abstract：:Interleukin 2 (IL2) mRNA has a short half-life in the cytoplasm of T lymphocytes, relative to most mRNA. We have discovered a candidate ribonuclease to account for the rapid turnover of IL2 mRNA in the cytosol of the human T lymphocyte cell line Jurkat. In partially purified form, this RNase is about 7 times as active...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.1.155
更新日期：1993-01-11 00:00:00
abstract：:The CRISPR system has become a powerful biological tool with a wide range of applications. However, improving targeting specificity and accurately predicting potential off-targets remains a significant goal. Here, we introduce a web-based CR: ISPR/Cas9 O: ff-target P: rediction and I: dentification T: ool (CROP-IT) th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv575
更新日期：2015-10-15 00:00:00
abstract：:DNA non-homologous end joining, the major mechanism for the repair of DNA double-strands breaks (DSB) in mammalian cells requires the DNA-dependent protein kinase (DNA-PK), a complex composed of a large catalytic subunit of 460 kDa (DNA-PKcs) and the heterodimer Ku70-Ku80 that binds to double-stranded DNA ends. Mutati...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.23.4778
更新日期：2000-12-01 00:00:00
abstract：:The apolipoprotein AI (apoAI) promoter/enhancer contains multiple cis -acting elements on which a variety of hepatocyte-enriched and ubiquitous transcription factors function synergistically to regulate liver-specific transcription. Adenovirus E1A proteins repress tissue-specific gene expression and disrupt the differ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.7.1761
更新日期：1998-04-01 00:00:00
abstract：:Three-dimensional structures have been solved for several naturally occurring RNA triple helices, although all are limited to six or fewer consecutive base triples, hindering accurate estimation of global and local structural parameters. We present an X-ray crystal structure of a right-handed, U•A-U-rich RNA triple he...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz1222
更新日期：2020-04-06 00:00:00
abstract：:Four phage clones which hybridize with U1 small nuclear RNA were obtained from a rat gene library. Two clones contain a presumed pseudogene. A third clone includes two gene candidates that are co-linear with the rat U1-RNA, 3.6kb apart and in the opposite orientation. The two genes are surrounded by identical sequence...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.6.1791
更新日期：1983-03-25 00:00:00
abstract：:Homologous recombination is essential for the preservation of genome stability, thereby preventing cancer. The recombination protein RAD51 drives DNA strand exchange, which requires the assembly, rearrangement and disassembly of a RAD51 filament on DNA, coupled to ATP binding and hydrolysis. This process is facilitate...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr295
更新日期：2011-08-01 00:00:00
abstract：:The RNA-Seq technology has revolutionized transcriptome characterization not only by accurately quantifying gene expression, but also by the identification of novel transcripts like chimeric fusion transcripts. The 'fusion' or 'chimeric' transcripts have improved the diagnosis and prognosis of several tumors, and have...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx315
更新日期：2017-07-27 00:00:00
abstract：:Trf4/5p-Air1/2p-Mtr4p polyadenylation complex (TRAMP) is an essential component of nuclear RNA surveillance in yeast. It recognizes a variety of nuclear transcripts produced by all three RNA polymerases, adds short poly(A) tails to aberrant or unstable RNAs and activates the exosome for their degradation. Despite the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks223
更新日期：2012-07-01 00:00:00
abstract：:We have characterized triple-helix-mediated inhibition of an artificial bacteriophage promoter with respect to relief of inhibition by incoming RNA polymerases that initiate upstream or downstream from the operator sequence. Whereas oligonucleotide-directed triple-helix formation inhibits the test promoter, promoter a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.17.4055
更新日期：1993-08-25 00:00:00
abstract：:Virulence factor database (VFDB) was set up in 2004 dedicated for providing current knowledge of virulence factors (VFs) from various medical significant bacterial pathogens to facilitate pathogenomic research. Nowadays, complete genome sequences of almost all the major pathogenic microbes have been determined, which ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm951
更新日期：2008-01-01 00:00:00
abstract：:The Pk-rec gene, encoding a RecA/RAD51 homologue from the hyperthermophilic archaeon Pyrococcussp. KOD1, was expressed in Escherichia coli. The recombinant Pk-REC was purified to homogeneity and was shown to be in a dimeric form. A striking property of the purified recombinant Pk-REC was the unusual DNase activity on ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.4.719
更新日期：1997-02-15 00:00:00
abstract：:Hepatitis B viruses, or hepadnaviruses, are small DNA-containing viruses that replicate through reverse transcription. Their prototype, HBV, causes severe liver disease in humans. The hepadnaviral P protein is an unusual reverse transcriptase (RT) that initiates DNA synthesis by host-factor-dependent protein priming o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.7.1679
更新日期：2002-04-01 00:00:00
abstract：:Stabilization of stalled replication forks prevents excessive fork reversal or degradation, which can undermine genome integrity. The WRN protein is unique among the other human RecQ family members to possess exonuclease activity. However, the biological role of the WRN exonuclease is poorly defined. Recently, the WRN...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz431
更新日期：2019-07-26 00:00:00
abstract：:The human 5S rRNA genes are found in clusters of tandem repeated units. We have cloned and partially characterized six restriction fragments from two clusters of 2.3 kb and 1.6 kb repeats, respectively. Four fragments from the cluster of 2.3 kb repeats contain a 5S rRNA gene and one fragment contains a gene variant wi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.15.4147
更新日期：1991-08-11 00:00:00
abstract：:The ability to store and interconnect all available information on proteins is crucial to modern biological research. Accordingly, the Universal Protein Resource (UniProt) plays an increasingly important role by providing a stable, comprehensive, freely accessible central resource on protein sequences and functional a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl929
更新日期：2007-01-01 00:00:00
abstract：:We have isolated and characterized a S. pombe promoter using a functional heterologous gene product assay. Random S. pombe genomic fragments were cloned upstream from the promoterless 'lacZ gene and tested in vivo for their efficiency to promote expression of the beta-galactosidase protein in the fission yeast. An eff...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.17.8603
更新日期：1988-09-12 00:00:00
abstract：:The genetic organization of the multiple ribosomal transcription units (RTUs) on the genome of the yeast Saccharomyces carlsbergensis was studied by electron microscopy of purified ribosomal DNA hybridized to 26S rRNA using the R-loop technique (Thomas, M., White, R.L. and Davis, R.W. (1973) Proc. Natl. Acad. Sci. U.S...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.8.2801
更新日期：1978-08-01 00:00:00
abstract：:The Ensembl project has been aggregating, processing, integrating and redistributing genomic datasets since the initial releases of the draft human genome, with the aim of accelerating genomics research through rapid open distribution of public data. Large amounts of raw data are thus transformed into knowledge, which...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1098
更新日期：2018-01-04 00:00:00
