Hormonal regulation of transcription of rDNA: use of nucleoside thiotriphosphates to measure initiation in isolated nuclei.

Abstract:

:Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (beta-S)triphosphates. Initiation of pre-rRNA was completely resistant to alpha-amanitin but was inhibited by either actinomycin D or heparin. Experiments with P1798 mouse lymphoma cells indicated that the antiproliferative effects of glucocorticoids on lymphoid cells includes a reduction in the ability of nuclei to initiate pre-rRNA chains.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Zhang ZY,Thompson EA,Stallcup MR

doi

10.1093/nar/12.21.8115

subject

Has Abstract

pub_date

1984-11-12 00:00:00

pages

8115-28

issue

21

eissn

0305-1048

issn

1362-4962

journal_volume

12

pub_type

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