An RNasin-resistant ribonuclease selective for interleukin 2 mRNA.

abstract：:The assembly of the complex that forms over the simian virus 40 origin to initiate DNA replication is not well understood. This complex is composed of the virus-coded T antigen and three cellular proteins, replication protein A (RPA), DNA polymerase alpha/primase (pol/prim) and topoisomerase I (topo I) in association ...

journal_title：Nucleic acids research

authors： Simmons DT,Gai D,Parsons R,Debes A,Roy R

更新日期：2004-02-11 00:00:00

abstract：:Reversible methylation of the N6 or N1 position of adenine in RNA has recently been shown to play significant roles in regulating the functions of RNA. RNA can also be alkylated upon exposure to endogenous and exogenous alkylating agents. Here we examined how regio-specific methylation at the hydrogen bonding edge of ...

journal_title：Nucleic acids research

authors： You C,Dai X,Wang Y

更新日期：2017-09-06 00:00:00

abstract：:In eukaryotes, exposure to stress conditions causes a shift from cap-dependent to cap-independent translation. In trypanosomatids, environmental switches are the driving force of a developmental program of gene expression, but it is yet unclear how their translation machinery copes with their constantly changing envir...

journal_title：Nucleic acids research

authors： Zinoviev A,Léger M,Wagner G,Shapira M

更新日期：2011-10-01 00:00:00

abstract：:Patterns in biological sequences frequently signify interesting features in the underlying molecule. Many tools exist to search for well-known patterns. Less support is available for exploratory analysis, where no well-defined patterns are known yet. PatScanUI (https://patscan.secondarymetabolites.org/) provides a hig...

journal_title：Nucleic acids research

authors： Blin K,Wohlleben W,Weber T

更新日期：2018-07-02 00:00:00

abstract：:The ARF tumour suppressor stabilizes p53 by negatively regulating the E3 ubiquitin ligase MDM2 to promote cell cycle arrest and cell death. However, ARF is also able to arrest cell proliferation by inhibiting ribosome biogenesis. In greater part this is achieved by targeting the transcription termination factor I (TTF...

journal_title：Nucleic acids research

authors： Lessard F,Stefanovsky V,Tremblay MG,Moss T

更新日期：2012-07-01 00:00:00

abstract：:Large expansions of the trinucleotide repeat GAA*TTC within the first intron of the X25 (frataxin) gene cause Friedreich's ataxia, the most common inherited ataxia. Expansion leads to reduced levels of frataxin mRNA in affected individuals. Here we show that GAA*TTC tracts, in the absence of any other frataxin gene se...

journal_title：Nucleic acids research

authors： Grabczyk E,Usdin K

更新日期：2000-07-15 00:00:00

abstract：:In the fission yeast Schizosaccharomyces pombe, the cdc11 gene is required for the initiation of septum formation at the end of mitosis. The sce3 gene was cloned as a multi-copy suppressor of the heat-sensitive mutant cdc11-136. When over-expressed, it rescues all mutants of cdc11 and also a heat-sensitive allele of c...

journal_title：Nucleic acids research

authors： Schmidt S,Hofmann K,Simanis V

更新日期：1997-09-01 00:00:00

abstract：:The frequencies of occurrence of four bases in the first, second and third codon positions and in the total coding sequences have been calculated by the codon usage table published in 1990 by Ikemura et al. The distribution of frequencies are further analysed in detail by a graphic technique presented recently by us. ...

journal_title：Nucleic acids research

authors： Zhang CT,Zhang R

更新日期：1991-11-25 00:00:00

abstract：:Peroxisomes are induced in Saccharomyces cerevisiae when this yeast is grown in the presence of oleate, and are repressed when glucose is supplied as the carbon source. Concomitant with this is an induction/repression of peroxisomal beta-oxidation enzymes. We are investigating the transcriptional control of acyl-CoA o...

journal_title：Nucleic acids research

authors： Wang TW,Lewin AS,Small GM

更新日期：1992-07-11 00:00:00

abstract：:Non-coding RNA regulatory elements are important for viral replication, making them promising targets for therapeutic intervention. However, regulatory RNA is challenging to detect and characterise using classical structure-function assays. Here, we present in cell Mutational Interference Mapping Experiment (in cell M...

journal_title：Nucleic acids research

authors： Smyth RP,Smith MR,Jousset AC,Despons L,Laumond G,Decoville T,Cattenoz P,Moog C,Jossinet F,Mougel M,Paillart JC,von Kleist M,Marquet R

更新日期：2018-05-18 00:00:00

abstract：:We had shown previously (3) that the E. coli argE gene could be turned-on by an IS3 element inserted in orientation II near the 5' end of the gene. Here we show that this effect is due to the presence of an outward promoter located on IS3. The exact site of insertion of IS3 was determined by DNA sequencing. Using the ...

journal_title：Nucleic acids research

authors： Charlier D,Piette J,Glansdorff N

更新日期：1982-10-11 00:00:00

abstract：:Base compositions were examined at every position in codons of more than 50 genes from taxonomically different bacteria and of the corresponding antisense sequences on the bacterial genes. We propose that the nonstop frame on antisense strand [NSF(a)] of GC-rich bacterial genes is the most promising sequence for newly...

journal_title：Nucleic acids research

authors： Ikehara K,Amada F,Yoshida S,Mikata Y,Tanaka A

更新日期：1996-11-01 00:00:00

abstract：:The solution structure of the complex between 4', 6-diamidino-2-phenylindole (DAPI) and DNA oligomer [d(GCGATTCGC)]2, containing a central T.T mismatch, has been characterized by combined use of proton one- and two-dimensional NMR spectroscopy, molecular mechanics and molecular dynamics computations including relaxati...

journal_title：Nucleic acids research

authors： Trotta E,Paci M

更新日期：1998-10-15 00:00:00

abstract：:Actin is a major cytoskeletal protein in eukaryotes. Recent studies suggest more diverse functional roles for this protein. Actin mRNA is known to be localized to neuronal synapses and undergoes rapid deadenylation during early developmental stages. However, its 3'-untranslated region (UTR) is not characterized and th...

journal_title：Nucleic acids research

authors： Ghosh T,Soni K,Scaria V,Halimani M,Bhattacharjee C,Pillai B

更新日期：2008-11-01 00:00:00

abstract：:Previous studies have implicated the DE-1 (-111/-106) and alpha A-CRYBP1 (-66/-57) sites for activity of the mouse alpha A-crystallin promoter in transiently transfected lens cells. Here we have used the bacterial chloramphenicol acetyltransferase (CAT) reporter gene to test the functional importance of the putative D...

journal_title：Nucleic acids research

authors： Sax CM,Ilagan JG,Piatigorsky J

更新日期：1993-06-11 00:00:00

abstract：:DNA polymerase gamma from purified nuclei of EMT-6 cells (mice) seems to be identical to the mitochondrial DNA polymerase from the same source following several criteria. These two enzyme activities are strongly inhibited by ethidium bromide and acriflavin, while proflavin, acridine orange, daunomycin and chloroquine ...

journal_title：Nucleic acids research

authors： Tarrago-Litvak L,Viratelle O,Darriet D,Dalibart R,Graves PV,Litvak S

更新日期：1978-06-01 00:00:00

abstract：:The activation of a telomere maintenance mechanism is required for cancer development in humans. While most tumors achieve this by expressing the enzyme telomerase, a fraction (5-15%) employs a recombination-based mechanism termed alternative lengthening of telomeres (ALT). Here we show that loss of the single-strande...

journal_title：Nucleic acids research

authors： Grudic A,Jul-Larsen A,Haring SJ,Wold MS,Lønning PE,Bjerkvig R,Bøe SO

更新日期：2007-01-01 00:00:00

abstract：:The rabbit homolog to the locus control region (LCR) of the human beta-like globin gene cluster was isolated, and long segments containing the DNase I hypersensitive sites (HS) were sequenced. The order and spacing of HS4, HS3, HS2 and HS1 are conserved between rabbit and human. Alignment of these sequences with their...

journal_title：Nucleic acids research

authors： Hardison R,Xu J,Jackson J,Mansberger J,Selifonova O,Grotch B,Biesecker J,Petrykowska H,Miller W

更新日期：1993-03-11 00:00:00

abstract：:RNase J is a conserved ribonuclease that belongs to the β-CASP family of nucleases. It possesses both endo- and exo-ribonuclease activities, which play a key role in pre-rRNA maturation and mRNA decay. Here we report high-resolution crystal structures of Deinococcus radiodurans RNase J complexed with RNA or uridine 5'...

journal_title：Nucleic acids research

authors： Zhao Y,Lu M,Zhang H,Hu J,Zhou C,Xu Q,Ul Hussain Shah AM,Xu H,Wang L,Hua Y

更新日期：2015-06-23 00:00:00

abstract：:Five peaks of DNA glycosylase activity showing a preference for MNNG alkylated DNA have been identified from extracts of adapted M. luteus. They are numerically designated as GI to GV in order of their decreasing molecular weights. The first two of these peaks have been highly purified. GI, is a constitutive heat labi...

journal_title：Nucleic acids research

authors： Riazuddin S,Athar A,Ahmed Z,Lali SM,Sohail A

更新日期：1987-08-25 00:00:00

abstract：:The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a t...

journal_title：Nucleic acids research

authors： Chrisey LA,Lee GU,O'Ferrall CE

更新日期：1996-08-01 00:00:00

abstract：:The sxy (tfoX) gene product is the central regulator of DNA uptake by naturally competent gamma-proteobacteria such as Haemophilus influenzae, Vibrio cholerae and probably Escherichia coli. However, the mechanisms regulating sxy gene expression are not understood despite being key to understanding the physiological ro...

journal_title：Nucleic acids research

authors： Cameron AD,Volar M,Bannister LA,Redfield RJ

更新日期：2008-01-01 00:00:00

abstract：:DNA double-strand breaks (DSBs) and their repair can cause extensive epigenetic changes. As a result, DSBs have been proposed to promote transcriptional and, ultimately, physiological dysfunction via both cell-intrinsic and cell-non-autonomous pathways. Studying the consequences of DSBs in higher organisms has, howeve...

journal_title：Nucleic acids research

authors： Kim J,Sturgill D,Tran AD,Sinclair DA,Oberdoerffer P

更新日期：2016-04-20 00:00:00

abstract：:Small inverted repeats (small palindromes) on plasmids have been shown to mediate a recombinational rearrangement event in Escherichia coli leading to the formation of inverted dimers (giant palindromes). This recombinational rearrangement event is efficient and independent of RecA and RecBCD. In this report, we propo...

journal_title：Nucleic acids research

authors： Lin CT,Lyu YL,Liu LF

更新日期：1997-08-01 00:00:00

abstract：:We have generated transgenic mice that express a diverse repertoire of human sequence immunoglobulins. The expression of this repertoire is directed by light and heavy chain minilocus transgenes comprised of human protein coding sequences in an unrearranged, germ-line configuration. In this paper we describe the const...

journal_title：Nucleic acids research

authors： Taylor LD,Carmack CE,Schramm SR,Mashayekh R,Higgins KM,Kuo CC,Woodhouse C,Kay RM,Lonberg N

更新日期：1992-12-11 00:00:00

abstract：:Correlation of motif occurrences with gene expression intensity is an effective strategy for elucidating transcriptional cis-regulatory logic. Here we demonstrate that this approach can also identify cis-regulatory elements for alternative pre-mRNA splicing. Using data from a human exon microarray, we identified 56 ca...

journal_title：Nucleic acids research

authors： Das D,Clark TA,Schweitzer A,Yamamoto M,Marr H,Arribere J,Minovitsky S,Poliakov A,Dubchak I,Blume JE,Conboy JG

更新日期：2007-01-01 00:00:00

abstract：:Gene-order-based comparison of multiple genomes provides signals for functional analysis of genes and the evolutionary process of genome organization. Gene clusters are regions of co-localized genes on genomes of different species. The rapid increase in sequenced genomes necessitates bioinformatics tools for finding g...

journal_title：Nucleic acids research

authors： Winter S,Jahn K,Wehner S,Kuchenbecker L,Marz M,Stoye J,Böcker S

更新日期：2016-11-16 00:00:00

abstract：:Transcriptional co-activator LEDGF/p75 is the major cellular interactor of HIV-1 integrase (IN), critical to efficient viral replication. In this work, a series of INs from the Betaretrovirus, Gammaretrovirus, Deltaretrovirus, Spumavirus and Lentivirus retroviral genera were tested for interaction with the host factor...

journal_title：Nucleic acids research

authors： Cherepanov P

更新日期：2007-01-01 00:00:00

abstract：:Minimized trans-acting HDV ribozyme systems consisting of three (Rz-3) and two (Rz-2) RNA strands were prepared and their folding conformations were analyzed by NMR spectroscopy. The guanosine residues in one of the enzyme components of Rz-3 were labeled with 13C and 15N. Imino proton signals were assigned by analysis...

journal_title：Nucleic acids research

authors： Tanaka Y,Hori T,Tagaya M,Sakamoto T,Kurihara Y,Katahira M,Uesugi S

更新日期：2002-02-01 00:00:00

abstract：:The unsymmetrical cyanine dye thiazole orange homodimer (TOTO) binds to single-stranded DNA (ssDNA, M13mp18 ssDNA) to form a fluorescent complex that is stable under the standard conditions of electrophoresis. The stability of this complex is indistinguishable from that of the corresponding complex of TOTO with double...

journal_title：Nucleic acids research

authors： Rye HS,Glazer AN

更新日期：1995-04-11 00:00:00