Abstract:
:The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film formed on silica surfaces. A variety of aminosilanes, crosslinkers and treatment conditions have been tested to identify optimal conditions for DNA immobilization using this approach. The DNA films which result have been characterized using UV spectroscopy, water contact angle measurement, radiolabeling and hybridization methods.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Chrisey LA,Lee GU,O'Ferrall CEdoi
10.1093/nar/24.15.3031subject
Has Abstractpub_date
1996-08-01 00:00:00pages
3031-9issue
15eissn
0305-1048issn
1362-4962pii
6j0113journal_volume
24pub_type
杂志文章abstract::Oligo(dG).oligo(dC)- or short poly(dG).poly(dC)-containing fragments were enriched and cloned by means of Mg2+-dependent triplex affinity capture and subsequent cloning procedures. A library constructed after three cycles of enrichment showed that approximately 80% of the clones in the supercoiled form formed a comple...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.9.1701
更新日期:1997-05-01 00:00:00
abstract::For the rapid and sensitive detection of p53 'hot spot' mutations, we combined polymerase chain reaction based single-strand conformational polymorphism (PCR-SSCP) analysis with sequence specific-clamping by peptide nucleic acids (PNAs) in a one-step reaction tube protocol. For this purpose, we designed two PNA molecu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.5.1356
更新日期:1998-03-01 00:00:00
abstract::An open reading frame upstream from nifHDK operon of Klebsiella pneumoniae had been described. The orientation of this open reading frame is opposite to that of nifHDK and sequence homology was found between the open reading frame promoter and the promoter of nifHDK operon. A recombinant plasmid carrying the promoter ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.12.4241
更新日期:1983-06-25 00:00:00
abstract::We have previously shown that integration of a polyoma vector containing rodent repetitive elements into rat cellular DNA is non-random (Wallenburg et al. J. Virol. 50: 678-683). Junctions between the polyoma vector and the host DNA occur in the repetitive sequences of the vector about ten times more frequently than w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.19.7849
更新日期:1987-10-12 00:00:00
abstract::Genome-wide transcriptome profiling has enabled non-supervised classification of tumours, revealing different sub-groups characterized by specific gene expression features. However, the biological significance of these subtypes remains for the most part unclear. We describe herein an interactive platform, Minimum Span...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx338
更新日期:2017-07-27 00:00:00
abstract::The purpose of this work was to analyse at the molecular level the DNA recognized by the antibodies to Z-DNA in in situ experiments. Antibodies to Z-DNA interact strongly with R-band positive heterochromatic segments of fixed metaphase chromosomes of Cebus (Viegas-Pequignot et al., 1983). These segments are constitute...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.8.3197
更新日期:1986-04-25 00:00:00
abstract::IGF2 mRNA-binding protein 1 (IMP1) is a key regulator of messenger RNA (mRNA) metabolism and transport in organismal development and, in cancer, its mis-regulation is an important component of tumour metastasis. IMP1 function relies on the recognition of a diverse set of mRNA targets that is mediated by the combinator...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz136
更新日期:2019-05-07 00:00:00
abstract::Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the resp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.18.3653
更新日期:1996-09-15 00:00:00
abstract::The nucleotide sequence of the promoter region and the first five genes of the atp (or unc) operon of Escherichia coli has been determined. The first proposed gene in the operon contains four AUA codons and may be poorly expressed; it encodes a basic but yet hydrophobic protein which could function as a pilot protein ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.16.3919
更新日期:1981-08-25 00:00:00
abstract::We present a theory of pluralistic and stochastic gene regulation. To bridge the gap between empirical studies and mathematical models, we integrate pre-existing observations with our meta-analyses of the ENCODE ChIP-Seq experiments. Earlier evidence includes fluctuations in levels, location, activity, and binding of ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw042
更新日期:2016-06-02 00:00:00
abstract::DEAD-box helicases catalyze the non-processive unwinding of double-stranded RNA (dsRNA) at the expense of adenosine triphosphate (ATP) hydrolysis. Nucleotide and RNA binding and unwinding are mediated by the RecA domains of the helicase core, but their cooperation in these processes remains poorly understood. We there...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku747
更新日期:2014-01-01 00:00:00
abstract::Replication of the promiscuous plasmid pMV158 requires expression of the initiator repB gene, which is controlled by the repressor CopG. Genes repB and copG are co-transcribed from promoter P(cr). We have studied the interactions between RNA polymerase, CopG and the promoter to elucidate the mechanism of repression by...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp503
更新日期:2009-08-01 00:00:00
abstract::HotSpot Wizard is a web server used for the automated identification of hotspots in semi-rational protein design to give improved protein stability, catalytic activity, substrate specificity and enantioselectivity. Since there are three orders of magnitude fewer protein structures than sequences in bioinformatic datab...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky417
更新日期:2018-07-02 00:00:00
abstract::The ability to store and interconnect all available information on proteins is crucial to modern biological research. Accordingly, the Universal Protein Resource (UniProt) plays an increasingly important role by providing a stable, comprehensive, freely accessible central resource on protein sequences and functional a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl929
更新日期:2007-01-01 00:00:00
abstract::The FREP database (http://facts.gsc.riken.go.jp/FREP/) contains 31 396 RepeatMasker-identified non-redundant variant repeat sequences derived from 16,527 mouse cDNAs with protein-coding potential. The repeats were computationally associated with potential effects on transcriptional variation, translation, protein func...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh123
更新日期:2004-01-01 00:00:00
abstract::Homologous recombination provides a means for the in vivo construction of recombinant DNA molecules that may be problematic to assemble in vitro. We have investigated the efficiency of recombination within the Caenorhabditis elegans germ line as a function of the length of homology between recombining molecules. Our f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm857
更新日期:2007-01-01 00:00:00
abstract::Non-coding genetic variants/mutations can play functional roles in the cell by disrupting regulatory interactions between transcription factors (TFs) and their genomic target sites. For most human TFs, a myriad of DNA-binding models are available and could be used to predict the effects of DNA mutations on TF binding....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz363
更新日期:2019-07-02 00:00:00
abstract::We report a novel system for efficient and specific targeted delivery of large nucleic acids to and into cells. Plasmid DNA and core histones were assembled to chromatin by salt gradient dialysis and subsequently connected to bispecific antibody derivatives (bsAbs) via a nucleic acid binding peptide bridge. The result...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz137
更新日期:2019-06-04 00:00:00
abstract::A highly repetitive DNA sequence from tilapia (Oreochromis mossambicus/hornorum) has been cloned and sequenced. It is a tandemly arrayed sequence of 237 bp and constitutes 7% of the fish genome. The copy number of the repeat is approximately 3 x 10(5) per haploid genome. DNA sequence analysis of 7 cloned repeats revea...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.13.5071
更新日期:1989-07-11 00:00:00
abstract::Two types of different repetitive DNA sequences called RepMP1 and RepMP2 were identified in the genome of Mycoplasma pneumoniae. The number of these repeated elements, their nucleotide sequence and their localization on a physical map of the M. pneumoniae genome were determined. The results show that RepMP1 appears at...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.17.8337
更新日期:1988-09-12 00:00:00
abstract::We used the high resolution and accuracy of the Cambridge Structural Database (CSD) to provide detailed information regarding base pairing interactions of selected nucleobases. We searched for base pairs in which nucleobases interact with each other through two or more hydrogen bonds and form more or less planar struc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa629
更新日期:2020-09-04 00:00:00
abstract::Increased cytochrome P450 2E1 (CYP2E1) expression is the main cause of oxidative stress, which exacerbates alcoholic liver diseases (ALDs). Estrogen-related receptor gamma (ERRγ) induces CYP2E1 expression and contributes to enhancing alcohol-induced liver injury. Retinoic acid-related orphan receptor alpha (RORα) has ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1034
更新日期:2016-02-18 00:00:00
abstract::Sae2 cooperates with the Mre11-Rad50-Xrs2 (MRX) complex to initiate resection of DNA double-strand breaks (DSBs) and to maintain the DSB ends in close proximity to allow their repair. How these diverse MRX-Sae2 functions contribute to DNA damage resistance is not known. Here, we describe mre11 alleles that suppress th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky086
更新日期:2018-04-06 00:00:00
abstract::Differential melting profiles and denaturation maps are calculated for fdDNA whose sequence of nucleotides has been determined recently. The melting profiles for the total DNA and a number of its restriction fragments are compared with experimental data taken from literature. The comparison enables one to correlate a ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.7.2547
更新日期:1978-07-01 00:00:00
abstract::The Restriction-modification system AhdI contains two convergent transcription units, one with genes encoding methyltransferase subunits M and S and another with genes encoding the controller (C) protein and the restriction endonuclease (R). We show that AhdI transcription is controlled by two independent regulatory l...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1116
更新日期:2008-03-01 00:00:00
abstract::The numerous genomic sequences and ESTs released by the Arabidopsis thaliana Genome Initiative (AGI) have allowed a systematic and functional study of the DEAD box RNA helicase family. Sequencing and in silico analysis led to the characterization of 28 novel A. thaliana DEAD box RNA helicases forming a family of 32 me...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.2.628
更新日期:1999-01-15 00:00:00
abstract::The three-dimensional Poisson-Boltzmann equation for the distribution of counterion charge density around double helical DNA has been solved by an iterative procedure. These computations have been performed for 0.01M monovalent salt solutions. A systematic study of the ten possible sequences found among adjacent nucle...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.3.1479
更新日期:1986-02-11 00:00:00
abstract::The transactivator responsive region (TAR) present in the 5'-NTR of the HIV-1 genome represents a potential target for antiretroviral intervention and a model system for the development of specific inhibitors of RNA-protein interaction. Earlier, we have shown that an anti-TAR polyamide nucleotide analog (PNA(TAR)) con...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki743
更新日期:2005-08-02 00:00:00
abstract::DNA was purified from double minutes isolated from MTX-resistant EL4/8 mouse lymphoma cells, digested to completion with Bam H1 restriction endonuclease and cloned in lambda-1059. The properties of the library suggest that the DNA from which it was made was not detectably contaminated with non-dm chromosome material, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.21.6597
更新日期:1982-11-11 00:00:00
abstract::The Comparative Toxicogenomics Database (CTD; http://ctdbase.org/) is a premier public resource for literature-based, manually curated associations between chemicals, gene products, phenotypes, diseases, and environmental exposures. In this biennial update, we present our new chemical-phenotype module that codes chemi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky868
更新日期:2019-01-08 00:00:00