Abstract:
:Homologous recombination provides a means for the in vivo construction of recombinant DNA molecules that may be problematic to assemble in vitro. We have investigated the efficiency of recombination within the Caenorhabditis elegans germ line as a function of the length of homology between recombining molecules. Our findings indicate that recombination can occur between molecules that share only 10 bp of terminal homology, and that 25 bp is sufficient to mediate relatively high levels of recombination. Recombination occurs with lower efficiency when the location of the homologous segment is subterminal or internal. As in yeast, recombination can also be mediated by either single- or double-stranded bridging oligonucleotides. We find that ligation between cohesive ends is highly efficient and does not require that the ends be phosphorylated; furthermore, precise intermolecular ligation between injected molecules that have blunt ends can also occur within the germ line.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Kemp BJ,Hatzold J,Sternick LA,Cornman-Homonoff J,Whitaker JM,Tieu PJ,Lambie EJdoi
10.1093/nar/gkm857subject
Has Abstractpub_date
2007-01-01 00:00:00pages
e133issue
19eissn
0305-1048issn
1362-4962pii
gkm857journal_volume
35pub_type
杂志文章abstract::The human beta-globin LCR plays a key role in the transcriptional regulation of the beta-globin locus and comprises four erythroid specific DNase I hypersensitive sites, designated 5'HS1-4. We have now isolated genomic clones containing 5'HS3 and 5'HS4 of the mouse beta-globin LCR. 5'HS3 and 5'HS4 are located 15 kb an...
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journal_title:Nucleic acids research
pub_type: 杂志文章
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