Abstract:
:An updated list of DNA sequences cloned from the human genome over the past year (1991) is presented. Intended as a guide to clone availability, this list includes published reports of cDNA, genomic and synthetic probes comprising both gene and pseudogene sequences.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Schmidtke J,Cooper DNdoi
10.1093/nar/20.suppl.2181keywords:
subject
Has Abstractpub_date
1992-05-11 00:00:00pages
2181-98eissn
0305-1048issn
1362-4962journal_volume
20 Supplpub_type
杂志文章abstract::Cryptic splice sites are used only when use of a natural splice site is disrupted by mutation. To determine the features that distinguish authentic from cryptic 5' splice sites (5'ss), we systematically analyzed a set of 76 cryptic 5'ss derived from 46 human genes. These cryptic 5'ss have a similar frequency distribut...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg830
更新日期:2003-11-01 00:00:00
abstract::DISCOVER1 (DIStribution COunter VERsion 1) is a new program that can identify DNA motifs occurring with a high deviation from the expected frequency. The program generates families of patterns, each family having a common set of defined bases. Undefined bases are inserted amongst the defined bases in different ways, t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.22.5152
更新日期:1993-11-11 00:00:00
abstract::The binding of transposase (Tnp) to the specific Tn5 end sequences is the first dedicated reaction during transposition. In this study, comparative DNA-binding analyses were performed using purified full-length Tnp and a C-terminal deletion variant (delta369) that lacks the putative dimerization domain. The shape of t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.19.3790
更新日期:1996-10-01 00:00:00
abstract::Two new beta-lactoglobulin (BLG)/human serum albumin (HSA) hybrid gene vectors were constructed and tested for expression in COS-7 cells and in transgenic mice. The HSA sequences were inserted between the second and sixth BLG exons. Transient transfection experiments with these vectors as well as a series of additiona...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.4.602
更新日期:1996-02-15 00:00:00
abstract::We have developed a novel multiplex quantitative DNA-based target amplification method suitable for sensitive, specific and quantitative detection on microarray. This new method named NASBA Implemented Microarray Analysis (NAIMA) was applied to GMO detection in food and feed, but its application can be extended to all...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn524
更新日期:2008-10-01 00:00:00
abstract::When B cells differentiate into plasma cells, there is a large increase in the cellular content of mRNA coding for immunoglobulin. This increase cannot be fully accounted for by the increase in rate of transcription of the genes. We have investigated the possibility that the half-life of mu heavy chain mRNA increases ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.24.10439
更新日期:1989-12-25 00:00:00
abstract::Conformations of 8-bromo-2'-[unk]-triisopropylbenzenesulfonyladenosine ([unk]) and its 3'-[unk]-isomer ([unk]) in solution have been determined by the use of intramolecular nuclear Overhauser effects in (1)H NMR spectroscopy. Compound [unk] has been proved to have a conformation in which the adenosine and benzene ring...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.6.823
更新日期:1974-06-01 00:00:00
abstract::The transactivator Staf, which contains seven contiguous zinc fingers of the C(2)-H(2)type, exerts its effects on gene expression by binding to specific targets in vertebrate small nuclear RNA (snRNA) and snRNA-type gene promoters. Here, we have investigated the interaction of the Staf zinc finger domain with the opti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.10.2114
更新日期:2000-05-15 00:00:00
abstract::The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.15.3031
更新日期:1996-08-01 00:00:00
abstract::The relationship between the similarity of expression patterns for a pair of genes and interaction of the proteins they encode is demonstrated both for the simple genome of the bacteriophage T7 and the considerably more complex genome of the yeast Saccharomyces cerevisiae. Statistical analysis of large-scale gene expr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.17.3513
更新日期:2001-09-01 00:00:00
abstract::The methyltransferase, M.EcoKI, recognizes the DNA sequence 5'-AACNNNNNNGTGC-3' and methylates adenine at the underlined positions. DNA methylation has been shown by crystallography to occur via a base flipping mechanism and is believed to be a general mechanism for all methyltransferases. If no structure is available...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh531
更新日期:2004-04-23 00:00:00
abstract::Site-specific DNA double-strand breaks have been used to generate knock-in through the homology-dependent or -independent pathway. However, low efficiency and accompanying negative impacts such as undesirable indels or tumorigenic potential remain problematic. In this study, we present an enhanced reduced-risk genome ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa195
更新日期:2020-06-04 00:00:00
abstract::The TcSNP database (http://snps.tcruzi.org) integrates information on genetic variation (polymorphisms and mutations) for different stocks, strains and isolates of Trypanosoma cruzi, the causative agent of Chagas disease. The database incorporates sequences (genes from the T. cruzi reference genome, mRNAs, ESTs and ge...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn874
更新日期:2009-01-01 00:00:00
abstract::Long-range enhancers of transcription are a key component of the genomic regulatory architecture. Recent studies have identified bi-directionally transcribed RNAs emanating from these enhancers known as eRNAs. However, it remains unclear how tightly coupled eRNA production is with enhancer activity. Through our system...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1018
更新日期:2014-11-10 00:00:00
abstract::A library of three synthetic ribozymes with randomized arms, targeting NUX, GUX and NXG triplets, respectively, were used to identify ribozyme-accessible sites on the HIV-1 LTR transcript comprising positions -533 to 386. Three cleavable sites were identified at positions 109, 115 and 161. Ribozymes were designed agai...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.21.4059
更新日期:2000-11-01 00:00:00
abstract::Cell differentiation is a central process in development and in cancer growth and dissemination. OCT4 (POU5F1) and NANOG are essential for cell stemness and pluripotency; yet, the mechanisms that regulate their expression remain largely unknown. Repetitive elements account for almost half of the Human Genome; still, t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw095
更新日期:2016-06-02 00:00:00
abstract::Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoure...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv311
更新日期:2015-05-19 00:00:00
abstract::Previously, we have shown that all class-1 polypeptide release factors (RFs) share a common glycine-glycine-glutamine (GGQ) motif, which is critical for RF activity. Here, we subjected to site-directed mutagenesis two invariant amino acids, Gln185 and Arg189, situated in the GGQ minidomain of human eRF1, followed by d...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.19.3982
更新日期:2001-10-01 00:00:00
abstract::The HSV gene encoding ICP4 is negatively regulated and the HSV gene encoding thymidine kinase is positively regulated by ICP4 in vivo. We report that ICP4 is a component of a stable complex that contains protein and a sequence of approximately 28 nucleotides that span the ICP4 gene transcription initiation site. The a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.2.555
更新日期:1988-01-25 00:00:00
abstract::Plasmid DNA incubated in interphase Xenopus egg extracts is normally assembled into chromatin and then into synthetic nuclei which undergo one round of regulated replication. During a study of restriction endonuclease cut plasmid replication intermediates (RIs) by the Brewer-Fangman 2D gel electrophoresis technique, w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.10.2187
更新日期:2000-05-15 00:00:00
abstract::The principal early promoter of human papillomaviruses (HPVs), designated P97 in the case of HPV-16, contains four characteristically aligned cis-responsive elements, namely one binding site for Sp1, two for the viral E2 proteins, and the TATA box. The Sp1 binding site is needed to mediate activation of P97 by the rem...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.2.251
更新日期:1992-01-25 00:00:00
abstract::Human cytomegalovirus (HCMV) terminase is composed of subunits pUL56 (130 kDa) and pUL89 ( approximately 75 kDa), encoded by the UL56 and UL89 genes. In a recent investigation, we demonstrated that the main ATPase activity is associated with the large terminase subunit pUL56. The protein has two putative ATP-binding s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg229
更新日期:2003-03-01 00:00:00
abstract::We have modeled local DNA sequence parameters to search for DNA architectural motifs involved in transcription regulation and promotion within the Xenopus laevis ribosomal gene promoter and the intergenic spacer (IGS) sequences. The IGS was found to be shaped into distinct topological domains. First, intrinsic bends s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.18.3433
更新日期:2000-09-15 00:00:00
abstract::Alternative splicing produces more than one protein from the majority of genes and the rarer forms can have dominant functions. Instability of alternative transcripts can also hinder the study of regulation of gene expression by alternative splicing. To investigate the true extent of alternative splicing we have devel...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl592
更新日期:2006-01-01 00:00:00
abstract::Most type II restriction-modification (R-M) systems produce separate endonuclease (REase) and methyltransferase (MTase) proteins. After R-M genes enter a new cell, MTase activity must appear before REase or the host chromosome will be cleaved. Temporal control of these genes thus has life-or-death consequences. PvuII ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn1010
更新日期:2009-02-01 00:00:00
abstract::Owing to their great potentials in genetic code extension and the development of nucleic acid-based functional nanodevices, DNA duplexes containing HgII-mediated base pairs have been extensively studied during the past 60 years. However, structural basis underlying these base pairs remains poorly understood. Herein, w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1296
更新日期:2017-03-17 00:00:00
abstract::Release of RNA polymerase II (Pol II) from promoter-proximal pausing has emerged as a critical step regulating gene expression in multicellular organisms. The transition of Pol II into productive elongation requires the kinase activity of positive transcription elongation factor b (P-TEFb), which is itself under a str...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkw585
更新日期:2016-09-19 00:00:00
abstract::SNiPlay is a web-based tool for detection, management and analysis of genetic variants including both single nucleotide polymorphisms (SNPs) and InDels. Version 3 now extends functionalities in order to easily manage and exploit SNPs derived from next generation sequencing technologies, such as GBS (genotyping by sequ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv351
更新日期:2015-07-01 00:00:00
abstract::Given the inherent limitations of in silico studies relying solely on DNA sequence analysis, the functional characterization of mammalian promoters and associated cis-regulatory elements requires experimental support, which demands cloning and analysis of putative promoter regions. Focusing on human chromosome 21, we ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq402
更新日期:2010-10-01 00:00:00
abstract::The Human Genome Project has facilitated the sequencing of many species, yet the current Sanger method is too expensive, labor intensive and time consuming to accomplish medical resequencing of human genomes en masse. Of the 'next-generation' technologies, cyclic reversible termination (CRT) is a promising method with...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm689
更新日期:2007-01-01 00:00:00