Abstract:
:Release of RNA polymerase II (Pol II) from promoter-proximal pausing has emerged as a critical step regulating gene expression in multicellular organisms. The transition of Pol II into productive elongation requires the kinase activity of positive transcription elongation factor b (P-TEFb), which is itself under a stringent control by the inhibitory 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. Here, we provide an overview on stimulating Pol II pause release by P-TEFb and on sequestering P-TEFb into 7SK snRNP. Furthermore, we highlight mechanisms that govern anchoring of 7SK snRNP to chromatin as well as means that release P-TEFb from the inhibitory complex, and propose a unifying model of P-TEFb activation on chromatin. Collectively, these studies shine a spotlight on the central role of RNA binding proteins (RBPs) in directing the inhibition and activation of P-TEFb, providing a compelling paradigm for controlling Pol II transcription with a non-coding RNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
C Quaresma AJ,Bugai A,Barboric Mdoi
10.1093/nar/gkw585subject
Has Abstractpub_date
2016-09-19 00:00:00pages
7527-39issue
16eissn
0305-1048issn
1362-4962pii
gkw585journal_volume
44pub_type
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