Abstract:
:Plasmid DNA incubated in interphase Xenopus egg extracts is normally assembled into chromatin and then into synthetic nuclei which undergo one round of regulated replication. During a study of restriction endonuclease cut plasmid replication intermediates (RIs) by the Brewer-Fangman 2D gel electrophoresis technique, we have observed the formation of a strong spike of X-shaped DNA molecules in extracts that otherwise yield very little or no RIs. Formation of these joint molecules is also efficiently induced in replication-competent extracts upon inhibition of replication fork progression by aphidicolin. Although their electrophoretic properties are quite similar to those of Holliday junctions, 2D gels of doubly cut plasmids show that these junctions can link two plasmid molecules at any pair of DNA sequences, with no regard for sequence homology at the branch points. Neutral-neutral-alkaline 3D gels show that the junctions only contain single strands of parental size and no recombinant strands. A hemicatenane, in which one strand of a duplex is wound around one strand of another duplex, is the most likely structure to account for these observations. The mechanism of formation of these novel joint DNA molecules and their biological implications are discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Lucas I,Hyrien Odoi
10.1093/nar/28.10.2187keywords:
subject
Has Abstractpub_date
2000-05-15 00:00:00pages
2187-93issue
10eissn
0305-1048issn
1362-4962pii
gkd334journal_volume
28pub_type
杂志文章abstract::The effects of Mg++, Mn++, and KCl addition, individually and in combination, on the rate of DNA- and RNA-primed DNA synthesis by avian myeloblastosis virus DNA polymerase (reverse transcriptase) using a variety of natural and synthetic template-primer combinations were examined. Optimal divalent cation concentrations...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.6.1473
更新日期:1976-06-01 00:00:00
abstract::FASTQ has emerged as a common file format for sharing sequencing read data combining both the sequence and an associated per base quality score, despite lacking any formal definition to date, and existing in at least three incompatible variants. This article defines the FASTQ format, covering the original Sanger stand...
journal_title:Nucleic acids research
pub_type: 历史文章,杂志文章,评审
doi:10.1093/nar/gkp1137
更新日期:2010-04-01 00:00:00
abstract::A soluble cell-free extract containing RNA polymerase III and factors essential for selective transcription of the yeast SUP4-o tRNATyr gene was prepared from Saccharomyces cerevisiae cells. An intragenic promoter for yeast RNA polymerase III was identified within the yeast tRNATyr coding sequence by testing several s...
journal_title:Nucleic acids research
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doi:10.1093/nar/10.24.8127
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abstract::Inducible promoters are a central regulatory component in synthetic biology, metabolic engineering, and protein production for laboratory and commercial uses. Many of these applications utilize two or more exogenous promoters, imposing a currently unquantifiable metabolic burden on the living system. Here, we engineer...
journal_title:Nucleic acids research
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doi:10.1093/nar/gkaa734
更新日期:2020-09-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.2.689
更新日期:1979-02-01 00:00:00
abstract::The ribosomal frameshifting signal of the mouse embryonal carcinoma differentiation regulated (Edr) gene represents the sole documented example of programmed -1 frameshifting in mammalian cellular genes [Shigemoto,K., Brennan,J., Walls,E,. Watson,C.J., Stott,D., Rigby,P.W. and Reith,A.D. (2001), Nucleic Acids Res., 29...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki299
更新日期:2005-03-14 00:00:00
abstract::Scalable and cost-effective production of error-free DNA is critical to meet the increased demand for such DNA in the field of biological science. Methods based on 'Dial-out PCR' have enabled the high-throughput error-free DNA synthesis from a microarray-synthesized DNA pool by labeling with retrieval PCR tags, and re...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-04-20 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.21.8115
更新日期:1984-11-12 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkv1133
更新日期:2015-12-02 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/3.8.2079
更新日期:1976-08-01 00:00:00
abstract::The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3'-->5' helicase and 3'-->5' exonuclease activities. Recently, we showed that the exonuclease activity of WRN is greatly stimulated by the human Ku heterodimer protein. We have now mapped this interaction physically and functi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf482
更新日期:2002-08-15 00:00:00
abstract::A modification of previously described methods of electron microscopic gene mapping and of gene enrichment based on the avidin-biotin interaction is presented. The modification consists of coupling cytochrome-c instead of pentane diamine to the oxidized 2', 3' terminus of an RNA by Schiff base formation and BH-4 reduc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.2.385
更新日期:1978-02-01 00:00:00
abstract::Precursor tRNAAsp molecules, containing a 26-base 5' leader, were treated with diethylpyrocarbonate, 50% hydrazine or anhydrous hydrazine/3M NaCl and then subjected to processing by RNase P RNAs from Escherichia coli or Bacillus subtilis. Fully processed tRNAs and material not successfully cleaved by the catalytic RNA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.4.885
更新日期:1991-02-25 00:00:00
abstract::Short DNA sequence motifs have been identified in viral and cellular enhancers which represent the binding sites for a variety of trans- acting factors. One such HeLa cell factor, EBP1, has been purified and shown to bind to sequences in the SV40 enhancer. The PRDII element in the human beta-interferon gene regulatory...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.2.499
更新日期:1989-01-25 00:00:00
abstract::DNA assembly allows individual DNA constructs or libraries to be assembled quickly and reliably. Most methods are either: (i) Modular, easily scalable and suitable for combinatorial assembly, but leave undesirable 'scar' sequences; or (ii) bespoke (non-modular), scarless but less suitable for construction of combinato...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1182
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz452
更新日期:2019-07-02 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.18.3681
更新日期:1997-09-15 00:00:00
abstract::The entire intervening sequence of Tetrahymena thermophila ribosomal DNA has been determined. It is 413 nucleotides long and has the same splice junctions as those in T. pigmentosa. There is 93% homology between the intervening sequences in the two species, and 100% homology between their adjacent 26S RNA coding regio...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1982-05-11 00:00:00
abstract::Plant orthologs of the yeast sucrose non-fermenting (Snf1) kinase and mammalian AMP-activated protein kinase (AMPK) represent an emerging class of important regulators of metabolic and stress signalling. The catalytic alpha-subunits of plant Snf1-related kinases (SnRKs) interact in the yeast two-hybrid system with dif...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2019-05-21 00:00:00
abstract::A previously elaborated technique for fixing a chosen partially melted state of DNA with glyoxal was used in a study of the melting process of the replicative form (RF III) of phi X174 DNA. Electron-microscopic maps corresponding to five points of the melting curve of RF III were obtained and compared with the theoret...
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更新日期:1982-03-25 00:00:00
abstract::Optogenetic control of CRISPR-Cas9 systems has significantly improved our ability to perform genome perturbations in living cells with high precision in time and space. As new Cas orthologues with advantageous properties are rapidly being discovered and engineered, the need for straightforward strategies to control th...
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更新日期:2020-12-16 00:00:00
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journal_title:Nucleic acids research
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更新日期:2016-06-20 00:00:00
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journal_title:Nucleic acids research
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更新日期:2015-12-02 00:00:00
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更新日期:2018-09-19 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx210
更新日期:2017-05-19 00:00:00