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Flexibility and stabilization of HgII-mediated C:T and T:T base pairs in DNA duplex.

Abstract：

:Owing to their great potentials in genetic code extension and the development of nucleic acid-based functional nanodevices, DNA duplexes containing HgII-mediated base pairs have been extensively studied during the past 60 years. However, structural basis underlying these base pairs remains poorly understood. Herein, we present five high-resolution crystal structures including one first-time reported C-HgII-T containing duplex, three T-HgII-T containing duplexes and one native duplex containing T-T pair without HgII. Our structures suggest that both C-T and T-T pairs are flexible in interacting with the HgII ion with various binding modes including N3-HgII-N3, N4-HgII-N3, O2-HgII-N3 and N3-HgII-O4. Our studies also reveal that the overall conformations of the C-HgII-T and T-HgII-T pairs are affected by their neighboring residues via the interactions with the solvent molecules or other metal ions, such as SrII. These results provide detailed insights into the interactions between HgII and nucleobases and the structural basis for the rational design of C-HgII-T or T-HgII-T containing DNA nanodevices in the future.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Liu H,Cai C,Haruehanroengra P,Yao Q,Chen Y,Yang C,Luo Q,Wu B,Li J,Ma J,Sheng J,Gan J

doi

10.1093/nar/gkw1296

subject

Has Abstract

pub_date

2017-03-17 00:00:00

pages

2910-2918

issue

5

eissn

0305-1048

issn

1362-4962

pii

gkw1296

journal_volume

45

pub_type

杂志文章

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