A 6-fold difference in the half-life of immunoglobulin mu heavy chain mRNA in cell lines representing two stages of B cell differentiation.

abstract：:We have analyzed multiple recombinant DNA clones containing ribosomal RNA repeat units of the silkmoth, Bombyx mori. In combination with genomic DNA blots, analysis of these clones indicated that the rDNA repeat of B. mori is 10.8 kilobase pair in length and tandemly repeated in the genome, as reported by Manning et a...

journal_title：Nucleic acids research

authors： Lecanidou R,Eickbush TH,Kafatos FC

更新日期：1984-06-11 00:00:00

abstract：:Sets of genes expressed in the same tissue are believed to be under the regulation of a similar set of transcription factors, and can thus be assumed to contain similar structural patterns in their regulatory regions. Here we present a study of the structural patterns in promoters of genes expressed specifically in 26...

journal_title：Nucleic acids research

authors： Vandenbon A,Nakai K

更新日期：2010-01-01 00:00:00

abstract：:SynergyFinder (https://synergyfinder.fimm.fi) is a stand-alone web-application for interactive analysis and visualization of drug combination screening data. Since its first release in 2017, SynergyFinder has become a widely used web-tool both for the discovery of novel synergistic drug combinations in pre-clinical mo...

journal_title：Nucleic acids research

authors： Ianevski A,Giri AK,Aittokallio T

更新日期：2020-07-02 00:00:00

abstract：:Arylsulfatase, produced by Chlamydomonas reinhardtii during sulfur-limited growth, is secreted into the periplasmic space and is readily assayed using a chromogenic substrate. To assess the usefulness of the gene encoding arylsulfatase (ars) as a reporter gene in C. reinhardtii, we have fused the promoter region of th...

journal_title：Nucleic acids research

authors： Davies JP,Weeks DP,Grossman AR

更新日期：1992-06-25 00:00:00

abstract：:Few transcriptional regulatory networks have been described in non-model organisms. In Entamoeba histolytica seminal aspects of pathogenesis are transcriptionally controlled, however, little is known about transcriptional regulatory networks that effect gene expression in this parasite. We used expression data from tw...

journal_title：Nucleic acids research

authors： Hackney JA,Ehrenkaufer GM,Singh U

更新日期：2007-01-01 00:00:00

abstract：:We have constructed simple test-plasmids to study transcriptional enhancers in yeast. In this paper the reporter-gene is a plasmid borne deletion-substitution derivative (his-del4) of the Saccharomyces cerevisiae HIS3 gene in which the native promoter has been replaced by a dormant, susceptible pseudopromoter. We inve...

journal_title：Nucleic acids research

authors： Ciaramella M,Sacco M,Pulitzer JF

更新日期：1988-09-26 00:00:00

abstract：:The circadian clock comprises transcriptional feedback loops of clock genes. Cryptochromes are essential components of the negative feedback loop in mammals as they inhibit CLOCK-BMAL1-mediated transcription. We purified mouse CRY1 (mCRY1) protein complexes from Sarcoma 180 cells to determine their roles in circadian ...

journal_title：Nucleic acids research

authors： Hara Y,Onishi Y,Oishi K,Miyazaki K,Fukamizu A,Ishida N

更新日期：2009-03-01 00:00:00

abstract：:Cloned ribosomal DNA (rDNA) of Locusta migratoria was analyzed by restriction site mapping and SI nuclease experiments. The repeat unit is 18 kb long. The nontranscribed spacer region (NTS) is very large (11 kb) and homogeneous in length, but many of the restriction sites are heterogeneous among the repeat units. Two ...

journal_title：Nucleic acids research

authors： Schäfer M,Kunz W

更新日期：1985-02-25 00:00:00

abstract：:The presence of the C.C mispair in a defined duplex oligodeoxynucleotide enhanced its capacity to serve as a substrate for highly purified human DNA methyltransferase. Analysis of tritiated reaction products showed that the C.C mispair acted as a "methylation acceptor" in that it was itself rapidly methylated. The m5C...

journal_title：Nucleic acids research

authors： Smith SS,Hardy TA,Baker DJ

更新日期：1987-09-11 00:00:00

abstract：:The primary structure of the E. coli rpoC gene (5321 base pairs) coding the beta'-subunit of RNA polymerase as well as its adjacent segment have been determined. The structure analysis of the peptides obtained by cleavage of the protein with cyanogen bromide and trypsin has confirmed the amino acid sequence of the bet...

journal_title：Nucleic acids research

authors： Ovchinnikov YuA,Monastyrskaya GS,Gubanov VV,Guryev SO,Salomatina IS,Shuvaeva TM,Lipkin VM,Sverdlov ED

更新日期：1982-07-10 00:00:00

abstract：:DNA from healthy Malaysian newborns was studied on gene maps after digestion with different restriction endonucleases. Of 65 newborns, two were found to be carriers of two different variants of triplicated alpha-globin loci. In variant no. 1, found in an Malay, the three alpha-globin genes are in an elongated DNA frag...

journal_title：Nucleic acids research

authors： Lie-Injo LE,Herrera AR,Kan YW

更新日期：1981-08-11 00:00:00

abstract：:Bacterial RNase III plays important roles in the processing and degradation of RNA transcripts. A major goal is to identify the cleavage targets of this endoribonuclease at a transcriptome-wide scale and delineate its in vivo cleavage rules. Here we applied to Escherichia coli grown to either exponential or stationary...

journal_title：Nucleic acids research

authors： Altuvia Y,Bar A,Reiss N,Karavani E,Argaman L,Margalit H

更新日期：2018-11-02 00:00:00

abstract：:During S phase, the entire genome must be precisely duplicated, with no sections of DNA left unreplicated. Here, we develop a simple mathematical model to describe the probability of replication failing due to the irreversible stalling of replication forks. We show that the probability of complete genome replication i...

journal_title：Nucleic acids research

authors： Newman TJ,Mamun MA,Nieduszynski CA,Blow JJ

更新日期：2013-11-01 00:00:00

abstract：:The rates of acid-catalyzed hydrolysis of a hypermodified nucleoside, wyosine and its 5'-monophosphate were determined at various pH, temperature and buffer concentrations. The results show that despite distinct differences in structure and the glycosyl bond stability, the hydrolysis of wyosine proceeds via cleavage o...

journal_title：Nucleic acids research

authors： Golankiewicz B,Zielonacka-Lis E,Folkman W

更新日期：1985-04-11 00:00:00

abstract：:Many complex viruses package their genomes into empty protein shells and bacteriophages of the Cystoviridae family provide some of the simplest models for this. The cystoviral hexameric NTPase, P4, uses chemical energy to translocate single-stranded RNA genomic precursors into the procapsid. We previously dissected th...

journal_title：Nucleic acids research

authors： El Omari K,Meier C,Kainov D,Sutton G,Grimes JM,Poranen MM,Bamford DH,Tuma R,Stuart DI,Mancini EJ

更新日期：2013-11-01 00:00:00

abstract：:In addition to the cap-dependent mechanism, eukaryotic initiation of translation can occur by a cap-independent mechanism which directs ribosomes to defined start codons enabled by internal ribosome entry site (IRES) elements. IRES elements from poliovirus and encephalomyocarditis virus are often used to construct bi-...

journal_title：Nucleic acids research

authors： Hennecke M,Kwissa M,Metzger K,Oumard A,Kröger A,Schirmbeck R,Reimann J,Hauser H

更新日期：2001-08-15 00:00:00

abstract：:CRISPR/Cas9 is a powerful genome editing system but uncontrolled Cas9 nuclease expression triggers off-target effects and even in vivo immune responses. Inspired by synthetic biology, here we built a synthetic switch that self-regulates Cas9 expression not only in the transcription step by guide RNA-aided self-cleavag...

journal_title：Nucleic acids research

authors： Shen CC,Hsu MN,Chang CW,Lin MW,Hwu JR,Tu Y,Hu YC

更新日期：2019-02-20 00:00:00

abstract：:Immature oocytes from Xenopus laevis contain a 42S ribonucleoprotein particle (RNP) containing 5S RNA, tRNA, a 43 kDa protein, and a 48 kDa protein. A particle containing 5S RNA and the 43 kDa protein (p43-5S) liberated from the 42S particle upon brief treatment with urea can be purified by anion exchange chromatograp...

journal_title：Nucleic acids research

authors： Sands MS,Bogenhagen DF

更新日期：1991-04-25 00:00:00

abstract：:Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by blunt-end ligation into the Sal I site of plasmid pBR322 which had been repaired with DNA polymerase I to create Taq I sites on each side o...

journal_title：Nucleic acids research

authors： Cochet M,Perrin F,Gannon F,Krust A,Chambon P,McKnight GS,Lee DC,Mayo KE,Palmiter R

更新日期：1979-06-11 00:00:00

abstract：:DNA polymerase lambda (pol lambda) is a member of the X family DNA polymerases and is endowed with multiple enzymatic activities. In this work we investigated the in vitro miscoding properties of full-length, human pol lambda either in the absence or in the presence of the human auxiliary proteins proliferating cell n...

journal_title：Nucleic acids research

authors： Maga G,Shevelev I,Villani G,Spadari S,Hübscher U

更新日期：2006-03-06 00:00:00

abstract：:Pyrimidine adducts in cellular DNA arise from modification of the pyrimidine 5,6-double bond by oxidation, reduction or hydration. The biological outcome includes increased mutation rate and potential lethality. A major DNA N:-glycosylase responsible for the excision of modified pyrimidine bases is the base excision r...

journal_title：Nucleic acids research

authors： Yang H,Phan IT,Fitz-Gibbon S,Shivji MK,Wood RD,Clendenin WM,Hyman EC,Miller JH

更新日期：2001-02-01 00:00:00

abstract：:Mismatch repair is a highly conserved cellular pathway responsible for repairing mismatched dsDNA. Errors are detected by the MutS enzyme, which most likely senses altered mechanical property of damaged dsDNA rather than a specific molecular pattern. While the curved shape of dsDNA in crystallographic MutS/DNA structu...

journal_title：Nucleic acids research

authors： Bouchal T,Durník I,Illík V,Réblová K,Kulhánek P

更新日期：2020-11-18 00:00:00

abstract：:We have used DNase I footprinting to examine the association kinetics of GA-, GT- and CT-containing oligonucleotides with the target sequence (GGA)5GG. (CCT)5CC. These reactions are slow yielding bimolecular association rate constants between 50 and 2000 M-1s-1. We find that GT-containing oligonucleotides bind much fa...

journal_title：Nucleic acids research

authors： Paes HM,Fox KR

更新日期：1997-08-15 00:00:00

abstract：:The RecQL4 helicase is involved in the maintenance of genome integrity and DNA replication. Mutations in the human RecQL4 gene cause the Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. Mouse models and experiments in human and Xenopus have proven the N-terminal part of RecQL4 to be vital for cell growth. We ...

journal_title：Nucleic acids research

authors： Ohlenschläger O,Kuhnert A,Schneider A,Haumann S,Bellstedt P,Keller H,Saluz HP,Hortschansky P,Hänel F,Grosse F,Görlach M,Pospiech H

更新日期：2012-09-01 00:00:00

abstract：:Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein-sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2...

journal_title：Nucleic acids research

authors： Nguyen Q,Iritani A,Ohkita S,Vu BV,Yokoya K,Matsubara A,Ikeda KI,Suzuki N,Nakayashiki H

更新日期：2018-03-16 00:00:00

abstract：:Benzo[c]phenanthrene dihydrodiol epoxide (B[c] PhDE) is well known as an important environmental chemical carcinogen that preferentially modifies DNA in adenine residues. However, the molecular mechanism by which B[c]PhDE induces tumorigenesis is not fully understood. In this report, we demonstrate that DNA mismatch r...

journal_title：Nucleic acids research

authors： Wu J,Zhu BB,Yu J,Zhu H,Qiu L,Kindy MS,Gu L,Seidel A,Li GM

更新日期：2003-11-15 00:00:00

abstract：:To elucidate the response to oxidative stress in eukaryotic cells, the effect of an oxidized nucleotide, 8-oxo-2'-deoxyguanosine 5'-triphosphate (8-oxo-dGTP), generated from dGTP with an active oxygen, on DNA synthesis was studied using a cell-free DNA replication system derived from Xenopus egg lysates with a single-...

journal_title：Nucleic acids research

authors： Kai T,Matsunaga R,Eguchi M,Kamiya H,Kasai H,Suzuki M,Izuta S

更新日期：2002-01-15 00:00:00

abstract：:A key issue when designing and using DNA-targeting nucleases is specificity. Ideally, an optimal DNA-targeting tool has only one recognition site within a genomic sequence. In practice, however, almost all designer nucleases available today can accommodate one to several mutations within their target site. The ability...

journal_title：Nucleic acids research

authors： Juillerat A,Dubois G,Valton J,Thomas S,Stella S,Maréchal A,Langevin S,Benomari N,Bertonati C,Silva GH,Daboussi F,Epinat JC,Montoya G,Duclert A,Duchateau P

更新日期：2014-04-01 00:00:00

abstract：:The P. falciparum pPFrep20 repetitive element from the Palo Alto Uganda strain has been isolated and sequenced. The Palo Alto pPFrep20 repeat (pPFPArep20) has a clustered subtelomeric location and on chromosome 1 has been deleted from one end. Analysis of chromosome 1 from 5 other strains has revealed that pPFrep20 se...

journal_title：Nucleic acids research

authors： Patarapotikul J,Langsley G

更新日期：1988-05-25 00:00:00

abstract：:Genomic DNA methylation contributes substantively to transcriptional regulations that underlie mammalian development and cellular differentiation. Much effort has been made to decipher the molecular mechanisms governing the establishment and maintenance of DNA methylation patterns. However, little is known about genom...

journal_title：Nucleic acids research

authors： Xie H,Wang M,de Andrade A,Bonaldo Mde F,Galat V,Arndt K,Rajaram V,Goldman S,Tomita T,Soares MB

更新日期：2011-05-01 00:00:00