Molecular characterization of Mybbp1a as a co-repressor on the Period2 promoter.

abstract：:Generation of double-stranded cDNA during reverse transcription of a variety of mRNA molecules is well known to involve the formation of covalently linked antisense and sense strands in a hairpin configuration. In the present study we have examined the sequence of molecular events which occurs during cDNA synthesis fr...

journal_title：Nucleic acids research

authors： Volloch VZ,Schweitzer B,Rits S

更新日期：1994-12-11 00:00:00

abstract：:Laser-Raman spectra of poly(rI) show the formation of an ordered complex in aqueous solutions of high ionic strength. This structure exhibits the A-helix geometry, contains stacked bases and is apparently stabilized by specific hydrogen bonding involving hypoxanthine C6=0 groups. Thermal dissociation of the poly(rI) c...

journal_title：Nucleic acids research

authors： Chou CH,Thomas GJ Jr,Arnott S,Smith PJ

更新日期：1977-07-01 00:00:00

abstract：:We have isolated genomic clones from Xenopus borealis representing 3 different types of histone gene cluster. We show that the major type (H1, H2B, H2A, H4, H3), present at about 60-70 copies per haploid genome (1), is tandemly reiterated with a repeat length of 15 kb. In situ hybridization to mitotic chromosomes show...

journal_title：Nucleic acids research

authors： Turner PC,Bagenal EB,Vlad MT,Woodland HR

更新日期：1988-04-25 00:00:00

abstract：:The fine structure of the melting curve for the linear colE1 DNA has been obtained. To find the ColE1 DNA regions corresponding to peaks in the melting curve's fine structure, we fixed the melted DNA regions with glyoxal /12/. Electron-microscopic denaturation maps were obtained for nine temperature points within the ...

journal_title：Nucleic acids research

authors： Borovik AS,Kalambet YA,Lyubchenko YL,Shitov VT,Golovanov EI

更新日期：1980-09-25 00:00:00

abstract：:Protein-DNA recognition is a critical component of gene regulatory processes but the underlying molecular mechanisms are not yet completely understood. Whereas the DNA binding preferences of transcription factors (TFs) are commonly described using nucleotide sequences, the 3D DNA structure is recognized by proteins an...

journal_title：Nucleic acids research

authors： Dror I,Zhou T,Mandel-Gutfreund Y,Rohs R

更新日期：2014-01-01 00:00:00

abstract：:Protein-protein interactions regulate many essential enzymatic processes in the cell. Somatic mutations outside of an enzyme active site can therefore impact cellular function by disruption of critical protein-protein interactions. In our investigation of the cellular impact of the T304I cancer mutation of DNA Polymer...

journal_title：Nucleic acids research

authors： Fang Q,Andrews J,Sharma N,Wilk A,Clark J,Slyskova J,Koczor CA,Lans H,Prakash A,Sobol RW

更新日期：2019-07-09 00:00:00

abstract：:A normal human population has been screened for the existence of further restriction fragment length polymorphisms (RFLPs) in the clotting factor IX gene in addition to the TaqI polymorphism already characterised (1,2). Two polymorphic loci were found, both within 6 Kb of the TaqI polymorphism within the body of the f...

journal_title：Nucleic acids research

authors： Winship PR,Anson DS,Rizza CR,Brownlee GG

更新日期：1984-12-11 00:00:00

abstract：:We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of th...

journal_title：Nucleic acids research

authors： Nielsen JT,Arar K,Petersen M

更新日期：2006-04-13 00:00:00

abstract：:The dicistrovirus intergenic internal ribosome entry site (IGR IRES) directly recruits the ribosome and initiates translation using a non-AUG codon. A subset of IGR IRESs initiates translation in either of two overlapping open reading frames (ORFs), resulting in expression of the 0 frame viral structural polyprotein a...

journal_title：Nucleic acids research

authors： Ren Q,Au HH,Wang QS,Lee S,Jan E

更新日期：2014-08-01 00:00:00

abstract：:The DNA repair enzyme human uracil DNA glycosylase (UNG) scans short stretches of genomic DNA and captures rare uracil bases as they transiently emerge from the DNA duplex via spontaneous base pair breathing motions. The process of DNA scanning requires that the enzyme transiently loosen its grip on DNA to allow stoch...

journal_title：Nucleic acids research

authors： Friedman JI,Majumdar A,Stivers JT

更新日期：2009-06-01 00:00:00

abstract：:Gene rewiring is a common evolutionary phenomenon in nature that may lead to extinction for living organisms. Recent studies on synthetic biology demonstrate that cells can survive genetic rewiring. This survival (adaptation) is often linked to the stochastic expression of rewired genes with random transcriptional cha...

journal_title：Nucleic acids research

authors： Murakami Y,Matsumoto Y,Tsuru S,Ying BW,Yomo T

更新日期：2015-01-01 00:00:00

abstract：:DDBJ (http://www.ddbj.nig.ac.jp) collected and released 1 880 115 entries or 1 134 086 245 bases in the period from July 2006 to June 2007. The released data contains the high-throughput cDNAs of cricket and high-quality draft genome of medaka among others. Our computer system has been upgraded since March 2007. Anoth...

journal_title：Nucleic acids research

authors： Sugawara H,Ogasawara O,Okubo K,Gojobori T,Tateno Y

更新日期：2008-01-01 00:00:00

abstract：:Post-translational modifications contribute to the spliceosome dynamics by facilitating the physical rearrangements of the spliceosome. Here, we report USP15, a deubiquitinating enzyme, as a regulator of protein-protein interactions for the spliceosome dynamics. We show that PRP31, a component of U4 snRNP, is modified...

journal_title：Nucleic acids research

authors： Das T,Park JK,Park J,Kim E,Rape M,Kim EE,Song EJ

更新日期：2017-05-05 00:00:00

abstract：:The role of the enzyme poly(adenosine diphosphate-ribose) polymerase (PADPRP) in DNA repair at the level of the gene was investigated with human HeLa cells in which PADPRP antisense transcripts are inducible with dexamethasone. After such induction, the cellular content of PADPRP is reduced by 90%. DNA damage and its ...

journal_title：Nucleic acids research

authors： Stevnsner T,Ding R,Smulson M,Bohr VA

更新日期：1994-11-11 00:00:00

abstract：:During S phase, the entire genome must be precisely duplicated, with no sections of DNA left unreplicated. Here, we develop a simple mathematical model to describe the probability of replication failing due to the irreversible stalling of replication forks. We show that the probability of complete genome replication i...

journal_title：Nucleic acids research

authors： Newman TJ,Mamun MA,Nieduszynski CA,Blow JJ

更新日期：2013-11-01 00:00:00

abstract：:Understanding networks of protein-protein interactions constitutes an essential component on a path towards comprehensive description of cell function. Whereas efficient techniques are readily available for the initial identification of interacting protein partners, practical strategies are lacking for the subsequent ...

journal_title：Nucleic acids research

authors： Pajunen M,Turakainen H,Poussu E,Peränen J,Vihinen M,Savilahti H

更新日期：2007-01-01 00:00:00

abstract：:The sequence of 436 nucleotides around the region coding for 5.8S RNA in the Sciara coprophila rDNA transcription unit (1) has been determined. Regions coding for 5.8S and 2S RNAs have been identified; they are 80 - 90% homologous to the corresponding Drosophila sequences and are separated by a 22 nucleotide long spac...

journal_title：Nucleic acids research

authors： Jordan BR,Latil-Damotte M,Jourdan R

更新日期：1980-08-25 00:00:00

abstract：:Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli was transformed with ...

journal_title：Nucleic acids research

authors： Gubbins EJ,Maurer RA,Hartley JL,Donelson JE

更新日期：1979-03-01 00:00:00

abstract：:Exome sequencing strategy is promising for finding novel mutations of human monogenic disorders. However, pinpointing the casual mutation in a small number of samples is still a big challenge. Here, we propose a three-level filtration and prioritization framework to identify the casual mutation(s) in exome sequencing ...

journal_title：Nucleic acids research

authors： Li MX,Gui HS,Kwan JS,Bao SY,Sham PC

更新日期：2012-04-01 00:00:00

abstract：:Tight cell-cycle regulation of the histone H4-K20 methyltransferase PR-Set7 is essential for the maintenance of genome integrity. In mammals, this mainly involves the interaction of PR-Set7 with the replication factor PCNA, which triggers the degradation of the enzyme by the CRL4CDT2 E3 ubiquitin ligase. PR-Set7 is al...

journal_title：Nucleic acids research

authors： Zouaz A,Fernando C,Perez Y,Sardet C,Julien E,Grimaud C

更新日期：2018-04-06 00:00:00

abstract：:Here, we report a webserver for the improved SDM, used for predicting the effects of mutations on protein stability. As a pioneering knowledge-based approach, SDM has been highlighted as the most appropriate method to use in combination with many other approaches. We have updated the environment-specific amino-acid su...

journal_title：Nucleic acids research

authors： Pandurangan AP,Ochoa-Montaño B,Ascher DB,Blundell TL

更新日期：2017-07-03 00:00:00

abstract：:The unwinding of supercoiled phi X174 RFI DNA induced by the tumorigenic (+) and non-tumorigenic (-) enantiomers of trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) has been investigated by agarose slab-gel and ethidium titration tube gel electrophoresis. The differences in adduct conformat...

journal_title：Nucleic acids research

authors： Xu R,Birke S,Carberry SE,Geacintov NE,Swenberg CE,Harvey RG

更新日期：1992-12-11 00:00:00

abstract：:HeLa cell OTF-1 has been purified on the basis of its DNA binding activity and used to raise a polyclonal rabbit antiserum. This antiserum is shown to recognize both native and denatured OTF-1 from both human and a similar protein from Xenopus culture cells, but to react either more weakly or not at all with the lymph...

journal_title：Nucleic acids research

authors： Pruijn JM,van der Vliet PC,Dathan NA,Mattaj IW

更新日期：1989-03-11 00:00:00

abstract：:Since its discovery in 1979, left-handed Z-DNA has evolved from an in vitro curiosity to a challenging DNA structure with crucial roles in gene expression, regulation and recombination. A fundamental question that has puzzled researchers for decades is how the transition from B-DNA, the prevalent right-handed form of ...

journal_title：Nucleic acids research

authors： Moradi M,Babin V,Roland C,Sagui C

更新日期：2013-01-07 00:00:00

abstract：:Long non-coding RNAs (lncRNAs) play critical roles in diverse cellular processes; however, their involvement in many critical aspects of the immune response including the interferon (IFN) response remains poorly understood. To address this gap, we compared the global gene expression pattern of primary human hepatocyte...

journal_title：Nucleic acids research

authors： Kambara H,Niazi F,Kostadinova L,Moonka DK,Siegel CT,Post AB,Carnero E,Barriocanal M,Fortes P,Anthony DD,Valadkhan S

更新日期：2014-01-01 00:00:00

abstract：:Several cDNA clones were isolated from cDNA libraries constructed with mRNA longer than 28S RNA from the murine cell line PYS-2/12. The plasmids have inserts containing 1-1.2 kb of the ribosomal 5' external transcribed spacer followed by nearly 700 nt of sequence for 18S rRNA and ending with a B2 element (retroposon)....

journal_title：Nucleic acids research

authors： Oberbäumer I

更新日期：1992-02-25 00:00:00

abstract：:A crucial step in analyzing mRNA-Seq data is to accurately and efficiently map hundreds of millions of reads to the reference genome and exon junctions. Here we present OLego, an algorithm specifically designed for de novo mapping of spliced mRNA-Seq reads. OLego adopts a multiple-seed-and-extend scheme, and does not ...

journal_title：Nucleic acids research

authors： Wu J,Anczuków O,Krainer AR,Zhang MQ,Zhang C

更新日期：2013-05-01 00:00:00

abstract：:The lytic-lysogenic decision in bacteriophage 186 is governed by the 186 CI repressor protein in a unique way. The 186 CI is proposed to form a wheel-like oligomer that can mediate either wrapped or looped nucleoprotein complexes to provide the cooperative and competitive interactions needed for regulation. Although c...

journal_title：Nucleic acids research

authors： Wang H,Dodd IB,Dunlap DD,Shearwin KE,Finzi L

更新日期：2013-06-01 00:00:00

abstract：:Circular DNA can arise from all parts of eukaryotic chromosomes. In yeast, circular ribosomal DNA (rDNA) accumulates dramatically as cells age, however little is known about the accumulation of other chromosome-derived circles or the contribution of such circles to genetic variation in aged cells. We profiled circular...

journal_title：Nucleic acids research

authors： Prada-Luengo I,Møller HD,Henriksen RA,Gao Q,Larsen CE,Alizadeh S,Maretty L,Houseley J,Regenberg B

更新日期：2020-08-20 00:00:00

abstract：:Recent studies employing genome-wide approaches have provided an unprecedented view of the scope of L1 activities on structural variations in the human genome, and further reinforced the role of L1s as one of the major driving forces behind human genome evolution. The rapid identification of novel L1 elements by these...

journal_title：Nucleic acids research

authors： Xie Y,Rosser JM,Thompson TL,Boeke JD,An W

更新日期：2011-02-01 00:00:00