Abstract:
:The presence of the C.C mispair in a defined duplex oligodeoxynucleotide enhanced its capacity to serve as a substrate for highly purified human DNA methyltransferase. Analysis of tritiated reaction products showed that the C.C mispair acted as a "methylation acceptor" in that it was itself rapidly methylated. The m5C.G base pair also enhanced the capacity of the oligodeoxynucleotide to serve as a substrate for the enzyme. However, this complementary base pair was found to act as a "methylation director". That is, the presence of the m5C in one strand induced the enzyme to rapidly methylate at the cytosine residue on the opposite strand in an adjacent C.G base pair.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Smith SS,Hardy TA,Baker DJdoi
10.1093/nar/15.17.6899subject
Has Abstractpub_date
1987-09-11 00:00:00pages
6899-916issue
17eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract::Nucleosomes released from oviduct nuclei during brief micrococcal nuclease digestions are enriched in transcribed sequences (bloom K.S. and Anderson, J.N. (1978) Cell, 15, 141-150). Such nucleosomes released into this 1Sf supernatant fraction are enriched in proteins HMG14, 17 and a third lower molecular weight protei...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.12.2761
更新日期:1981-06-25 00:00:00
abstract::The eukaryotic linear motif (ELM) resource is a repository of manually curated experimentally validated short linear motifs (SLiMs). Since the initial release almost 20 years ago, ELM has become an indispensable resource for the molecular biology community for investigating functional regions in many proteins. In this...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1030
更新日期:2020-01-08 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.5.1683
更新日期:1988-03-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.22.6625
更新日期:1990-11-25 00:00:00
abstract::We have used gel retardation analysis to show that human DNA topoisomerase IIbeta can bind a 40 bp linear duplex containing a single DNA topoisomerase IIbeta cleavage site. Furthermore, we demonstrate for the first time that human DNA topoisomerase IIbeta binds to four-way junction DNA. This supports previous suggesti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.4.984
更新日期:1999-02-15 00:00:00
abstract::The finding in animal species of complexes homologous to the products of six Saccharomyces cerevisiae genes, origin of replication recognition complex (ORC), has suggested that ORC-related mechanisms have been conserved in all eukaryotes. In plants, however, the only cloned putative homologs of ORC subunits are the Ar...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg138
更新日期:2003-01-15 00:00:00
abstract::Yeast two-hybrid (Y2H) screenings result in identification of many out-of-frame (OOF) clones that code for short (2-100 amino acids) peptides with no sequence homology to known proteins. We hypothesize that these peptides can reveal common short linear motifs (SLiMs) responsible for their selection. We present a new p...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
abstract::The Integrated Microbial Genomes (IMG) system serves as a community resource for comparative analysis of publicly available genomes in a comprehensive integrated context. IMG integrates publicly available draft and complete genomes from all three domains of life with a large number of plasmids and viruses. IMG provide...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1044
更新日期:2012-01-01 00:00:00
abstract::The E. coli single-stranded binding protein (SSB) has been demonstrated in vitro to be involved in a number of replicative, DNA renaturation, and protective functions. It was shown previously that SSB can interact with exonuclease I to stimulate the hydrolysis of single-stranded DNA. We demonstrate here that E. coli S...
journal_title:Nucleic acids research
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更新日期:1994-01-25 00:00:00
abstract::DNA intercalators are found to recognize a DNA lesion as a high affinity receptor site. This lesion-specific binding is observed when one strand of a DNA double helix contains an extra, unpaired nucleotide. Our assay for binding controls for the effects of sequence with a series of oligodeoxynucleotide duplexes which ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.24.11607
更新日期:1988-12-23 00:00:00
abstract::Proteomic and RNomic approaches have identified many components of different ribonucleoprotein particles (RNPs), yet still little is known about the organization and protein proximities within these heterogeneous and highly dynamic complexes. Here we describe a targeted cross-linking approach, which combines cross-lin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1366
更新日期:2016-02-18 00:00:00
abstract::Oxoguanine DNA glycosylase (OGG1) initiates the repair of 8-oxoguanine (8-oxoG), a major oxidative DNA base modification that has been directly implicated in cancer and aging. OGG1 functions in the base excision repair pathway, for which a molecular hand-off mechanism has been proposed. To date, only one functional an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki636
更新日期:2005-06-07 00:00:00
abstract::LINE1s occupy 17% of the human genome and are its only active autonomous mobile DNA. L1s are also responsible for genomic insertion of processed pseudogenes and >1 million non-autonomous retrotransposons (Alus and SVAs). These elements have significant effects on gene organization and expression. Despite the importanc...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
abstract::The mitochondrial DNA (mtDNA) control regions for common chimpanzee, pygmy chimpanzee and gorilla were sequenced and the lengths and termini of their D-loop DNA's characterized. In these and all other species for which there are data, 5' termini map to sequences that contain the trinucleotide YAY. 3' termini are 25-51...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.13.5841
更新日期:1988-07-11 00:00:00
abstract::A newly discovered group of spherical plant viruses contains a bipartite genome consisting of a single-strand linear RNA molecule (RNA 1, Mr 1.5 x 10(6) ), and a single-strand, covalently closed circular viroid-like RNA molecule (RNA 2, Mr approximately 125,000). The nucleotide sequences of the RNA 2 of two of these, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.12.3681
更新日期:1982-06-25 00:00:00
abstract::ADGO 2.0 is a web-based tool that provides composite interpretations for microarray data comparing two sample groups as well as lists of genes from diverse sources of biological information. Some other tools also incorporate composite annotations solely for interpreting lists of genes but usually provide highly redund...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr392
更新日期:2011-07-01 00:00:00
abstract::Diastereomeric dithymidine methanephosphonamidates (TnpmT) were synthesized by reaction of 3'-amino-3'-deoxythymidine with 3'- O -acetylthymidin-5-yl-methanephosphonochloridate. Separated dinucleotide TnpmT(fast) and TnpmT(slow) diastereomers were used as building blocks to prepare chimeric dodecathy-midylates, posses...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.11.2650
更新日期:1998-06-01 00:00:00
abstract::Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented DNA, information traditionally lost in standard NGS library prepa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa128
更新日期:2020-05-07 00:00:00
abstract::A phage-display library of random peptides is a combinatorial experimental technique that can be harnessed for studying antibody-antigen interactions. In this technique, a phage peptide library is scanned against an antibody molecule to obtain a set of peptides that are bound by the antibody with high affinity. This s...
journal_title:Nucleic acids research
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更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2004-06-04 00:00:00
abstract::Mutations in the RecQ DNA helicase gene BLM give rise to Bloom's syndrome, which is a rare autosomal recessive disorder characterized by genetic instability and cancer predisposition. BLM helicase is highly active in binding and unwinding G-quadruplexes (G4s), which are physiological targets for BLM, as revealed by ge...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2015-01-01 00:00:00
abstract::The sequence of the 356 nucleotide residues of chrysanthemum stunt viroid (CSV) has been determined. Overlapping linear viroid fragments were obtained by partial ribonuclease digestion, radiolabelled in vitro at their 5'-ends, and sequenced using partial enzymic cleavage methods. Of the CSV sequence, 69% is contained ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1981-06-25 00:00:00
abstract::To understand the epigenetic regulation of transcriptional response of macrophages during early-stage M. tuberculosis (Mtb) infection, we performed ChIPseq analysis of H3K4 monomethylation (H3K4me1), a marker of poised or active enhancers. De novo H3K4me1 peaks in infected cells were associated with genes implicated i...
journal_title:Nucleic acids research
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更新日期:2016-12-15 00:00:00
abstract::G-quadruplex (G4) DNA structures can form physical barriers within the genome that must be unwound to ensure cellular genomic integrity. Here, we report unanticipated roles for the Escherichia coli Rep helicase and RecA recombinase in tolerating toxicity induced by G4-stabilizing ligands in vivo. We demonstrate that R...
journal_title:Nucleic acids research
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更新日期:2020-07-09 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1985-11-25 00:00:00
abstract::DNA assembly allows individual DNA constructs or libraries to be assembled quickly and reliably. Most methods are either: (i) Modular, easily scalable and suitable for combinatorial assembly, but leave undesirable 'scar' sequences; or (ii) bespoke (non-modular), scarless but less suitable for construction of combinato...
journal_title:Nucleic acids research
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更新日期:2019-02-20 00:00:00
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journal_title:Nucleic acids research
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更新日期:2019-07-09 00:00:00
abstract::Mitotic chromosome structure is pivotal to cell division but difficult to observe in fine detail using conventional methods. DNA catenation has been implicated in both sister chromatid cohesion and chromosome condensation, but has never been observed directly. We have used a lab-on-a-chip microfluidic device and fluor...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2017-08-21 00:00:00