Human DNA (cytosine-5)methyltransferase selectively methylates duplex DNA containing mispairs.

Abstract:

:The presence of the C.C mispair in a defined duplex oligodeoxynucleotide enhanced its capacity to serve as a substrate for highly purified human DNA methyltransferase. Analysis of tritiated reaction products showed that the C.C mispair acted as a "methylation acceptor" in that it was itself rapidly methylated. The m5C.G base pair also enhanced the capacity of the oligodeoxynucleotide to serve as a substrate for the enzyme. However, this complementary base pair was found to act as a "methylation director". That is, the presence of the m5C in one strand induced the enzyme to rapidly methylate at the cytosine residue on the opposite strand in an adjacent C.G base pair.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Smith SS,Hardy TA,Baker DJ

doi

10.1093/nar/15.17.6899

subject

Has Abstract

pub_date

1987-09-11 00:00:00

pages

6899-916

issue

17

eissn

0305-1048

issn

1362-4962

journal_volume

15

pub_type

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