Abstract:
:The primary structure of the E. coli rpoC gene (5321 base pairs) coding the beta'-subunit of RNA polymerase as well as its adjacent segment have been determined. The structure analysis of the peptides obtained by cleavage of the protein with cyanogen bromide and trypsin has confirmed the amino acid sequence of the beta'-subunit deduced from the nucleotide sequence analysis. The beta'-subunit of E. coli RNA polymerase contains 1407 amino acid residues. Its translation is initiated by codon GUG and terminated by codon TAA. It has been detected that the sequence following the terminating codon is strikingly homologous to known sequences of rho-independent terminators.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ovchinnikov YuA,Monastyrskaya GS,Gubanov VV,Guryev SO,Salomatina IS,Shuvaeva TM,Lipkin VM,Sverdlov EDdoi
10.1093/nar/10.13.4035subject
Has Abstractpub_date
1982-07-10 00:00:00pages
4035-44issue
13eissn
0305-1048issn
1362-4962journal_volume
10pub_type
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