Abstract:
:The National Center for Biotechnology Information (NCBI) provides a large suite of online resources for biological information and data, including the GenBank® nucleic acid sequence database and the PubMed® database of citations and abstracts published in life science journals. The Entrez system provides search and retrieval operations for most of these data from 34 distinct databases. The E-utilities serve as the programming interface for the Entrez system. Custom implementations of the BLAST program provide sequence-based searching of many specialized datasets. New resources released in the past year include a new PubMed interface and NCBI datasets. Additional resources that were updated in the past year include PMC, Bookshelf, Genome Data Viewer, SRA, ClinVar, dbSNP, dbVar, Pathogen Detection, BLAST, Primer-BLAST, IgBLAST, iCn3D and PubChem. All of these resources can be accessed through the NCBI home page at https://www.ncbi.nlm.nih.gov.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sayers EW,Beck J,Bolton EE,Bourexis D,Brister JR,Canese K,Comeau DC,Funk K,Kim S,Klimke W,Marchler-Bauer A,Landrum M,Lathrop S,Lu Z,Madden TL,O'Leary N,Phan L,Rangwala SH,Schneider VA,Skripchenko Y,Wang J,Ye J,doi
10.1093/nar/gkaa892subject
Has Abstractpub_date
2021-01-08 00:00:00pages
D10-D17issue
D1eissn
0305-1048issn
1362-4962pii
5937080journal_volume
49pub_type
杂志文章,评审abstract::Stabilization of stalled replication forks prevents excessive fork reversal or degradation, which can undermine genome integrity. The WRN protein is unique among the other human RecQ family members to possess exonuclease activity. However, the biological role of the WRN exonuclease is poorly defined. Recently, the WRN...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz431
更新日期:2019-07-26 00:00:00
abstract::Controllable gene delivery via vector-based systems remains a formidable challenge in mammalian synthetic biology and a desirable asset in gene therapy applications. Here, we introduce a methodology to control the copies and residence time of a gene product delivered in host human cells but also selectively disrupt fr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1326
更新日期:2015-01-01 00:00:00
abstract::Yeast co-expressing rat APOBEC-1 and a fragment of human apolipoprotein B (apoB) mRNA assembled functional editosomes and deaminated C6666 to U in a mooring sequence-dependent fashion. The occurrence of APOBEC-1-complementing proteins suggested a naturally occurring mRNA editing mechanism in yeast. Previously, a hidde...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.8.1772
更新日期:2001-04-15 00:00:00
abstract::We describe a method for anchoring bacteriophage lambda DNA by one end to gold by Au-biotin-streptavidin-biotin-DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.3.492
更新日期:1994-02-11 00:00:00
abstract::We have previously reported the human cDNA encoding MSSP-1, a sequence-specific double- and single-stranded DNA binding protein [Negishi, Nishita, Saëgusa, Kakizaki, Galli, Kihara, Tamai, Miyajima, Iguchi-Ariga and Ariga (1994) Oncogene, 9, 1133-1143]. MSSP-1 binds to a DNA replication origin/transcriptional enhancer ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.25.5576
更新日期:1994-12-25 00:00:00
abstract::A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are generated between PCR products and a PCR-amplified plasmid vector. The procedure does not require the use of restriction enzymes, T4 DNA ligase or ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.20.6069
更新日期:1990-10-25 00:00:00
abstract::Through a computer search of DNA databases, we have identified the homologs of the mgU6-47 snoRNA gene from the yeast Schizosaccharomyces pombe, the fly Drosophila melanogaster and human. The three box C/D-containing snoRNA genes showed no significant similarity in their sequences except for an 11 nt long complementar...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.4.894
更新日期:2002-02-15 00:00:00
abstract::Eukaryotic topoisomerase II (topo II) is the essential decatenase of newly replicated chromosomes and the main relaxase of nucleosomal DNA. Apart from these general tasks, topo II participates in more specialized functions. In mammals, topo IIα interacts with specific RNA polymerases and chromatin-remodeling complexes...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt707
更新日期:2013-11-01 00:00:00
abstract::The BRENDA enzyme information system (http://www.brenda-enzymes.org/) has developed into an elaborate system of enzyme and enzyme-ligand information obtained from different sources, combined with flexible query systems and evaluation tools. The information is obtained by manual extraction from primary literature, text...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1068
更新日期:2015-01-01 00:00:00
abstract::The nematode Caenorhabditis elegans contains a small family of vitellogenin genes which is expressed abundantly, but only in the intestine of the adult hermaphrodite worm. In order to identify possible regulatory elements, we have sequenced the DNA surrounding the 5' ends of five of the six genes. Contained within reg...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.14.5283
更新日期:1985-07-25 00:00:00
abstract::Sequence-specific binding of proteins to their DNA targets involves a complex spectrum of processes that often induce DNA conformational variation in the bound complex. The forces imposed by protein binding that cause the helical deformations are intimately interrelated and difficult to parse or rank in importance. To...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt492
更新日期:2013-08-01 00:00:00
abstract::The Department of Energy (DOE) Joint Genome Institute (JGI) is a national user facility with massive-scale DNA sequencing and analysis capabilities dedicated to advancing genomics for bioenergy and environmental applications. Beyond generating tens of trillions of DNA bases annually, the Institute develops and maintai...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr947
更新日期:2012-01-01 00:00:00
abstract::The frequencies of occurrence of four bases in the first, second and third codon positions and in the total coding sequences have been calculated by the codon usage table published in 1990 by Ikemura et al. The distribution of frequencies are further analysed in detail by a graphic technique presented recently by us. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.22.6313
更新日期:1991-11-25 00:00:00
abstract::The location of target DNA sequences within chromatin may affect the ability of trans-acting factors to bind cis-elements and regulate gene transcription. To examine the effect of chromatin structure on the ability of the estrogen-estrogen receptor complex (E2R) to bind its respective DNA binding element within the ra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.6.1065
更新日期:1996-03-15 00:00:00
abstract::We characterised the human hSuv3p protein belonging to the family of NTPases/helicases. In yeast mitochondria the hSUV3 orthologue is a component of the degradosome complex and participates in mtRNA turnover and processing, while in Caenorhabditis elegans the hSUV3 orthologue is necessary for viability of early embryo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf647
更新日期:2002-12-01 00:00:00
abstract::RNA editing by adensosine deaminases is a widespread mechanism to alter genetic information in metazoa. In addition to modifications in non-coding regions, editing contributes to diversification of protein function, in analogy to alternative splicing. However, although splicing programs respond to external signals, fa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1045
更新日期:2013-01-01 00:00:00
abstract::Achiral pseudonucleosides bearing an anthraquinone moiety have been incorporated into deoxyoligonucleotides in internal, 3' and 5' positions. The ability of these modified deoxyoligonucleotides to hybridize to complementary RNA and DNA was investigated using Tm measurements. The anthraquinone was shown to enhance bind...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.11.3111
更新日期:1991-06-11 00:00:00
abstract::Partial acid cleavage, comparative HPLC tryptic peptide mapping and amino acid sequencing of the C1 and C2 proteins of HeLa heterogeneous nuclear ribonucleoprotein (hnRNP) particles demonstrate that proteins C1 and C2 differ in primary structure by the presence of a 13 amino acid insert sequence in C2. This C2 insert ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.21.8441
更新日期:1989-11-11 00:00:00
abstract::Human telomerase reverse transcriptase (hTERT), the catalytic subunit of human telomerase, contains conserved motifs common to retroviral reverse transcriptases and telomerases. Within the C motif of hTERT is the Leu866-Val867-Asp868-Asp869 tetrapeptide that includes a catalytically essential aspartate dyad. Site-dire...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm002
更新日期:2007-01-01 00:00:00
abstract::Initiation of bacterial DNA replication at oriC is mediated by primosomal proteins that act cooperatively to melt an AT-rich region where the replicative helicase is loaded prior to the assembly of the replication fork. In Bacillus subtilis, the dnaD, dnaB and dnaI genes are essential for initiation of DNA replication...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1236
更新日期:2010-05-01 00:00:00
abstract::The resonance Raman spectrum of native DNA has been obtained using excitation at 257 nm. In a first part, the spectral lines are assigned to the different nucleotide bases which provide the resonance effect. In a second part, the interactions of DNA with basic peptides (Arginine Methylester, Lysine Methylester, Arginy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.5.1707
更新日期:1982-03-11 00:00:00
abstract::Aberrant CpG methylation changes occurring during tumour progression include the loss (hypomethylation) and gain (hypermethylation) of methyl groups. Techniques currently available for examining such changes either require selection of a region, then examination of methylation changes, or utilise methylation-sensitive...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.24.e123
更新日期:2001-12-15 00:00:00
abstract::To generate long arrays of nucleosomes within a topologically defined chromatin domain we have assembled minichromosomes on negatively supercoiled plasmid DNA with extracts from Drosophila preblastoderm embryos. These minichromosomes are dynamic substrates for energy-dependent nucleosome remodeling machines that facil...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.16.3657
更新日期:1998-08-15 00:00:00
abstract::Magnetic tweezers are a powerful single-molecule technique that allows real-time quantitative investigation of biomolecular processes under applied force. High pulling forces exceeding tens of picoNewtons may be required, e.g. to probe the force range of proteins that actively transcribe or package the genome. Frequen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku677
更新日期:2014-10-01 00:00:00
abstract::We describe improve enzymatic methods for sequencing method for sequencing DNA. They are based on partial digestion of duplex DNA with exonuclease III to produce DNA molecules with 3' ends shortened to varying lengths, followed by repair synthesis to extend and label the 3' ends. After asymmetrical cleavage of the DNA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.6.2065
更新日期:1982-03-25 00:00:00
abstract::Previous studies have shown that (i) several RNAs induced by heat shock of Drosophila melanogaster cells are homologous to tandemly repeated alpha beta units found in cloned segments of D. melanogaster DNA, and (ii) the alpha beta sequences are present both at a major heat shock locus, 87Cl, and the chromocenter of po...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.20.5297
更新日期:1981-10-24 00:00:00
abstract::We have isolated the gene coding for the U2 analogue in trypanosomes. The 148 nucleotide long U2 RNA is capped and transcribed from a single copy gene. The 5' half of the molecule is highly homologous to mammalian U2 RNA, while the 3' half does not show any significant sequence homology with the mammalian counterpart....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.22.8893
更新日期:1986-11-25 00:00:00
abstract::PIWI-interacting RNAs (piRNAs) engage PIWI proteins to silence transposons and promote germ cell development in animals. In diverse species, piRNA biogenesis occurs near the mitochondrial surface, and involves mitochondrial membrane-anchored factors. In mice, two cytoplasmic PIWI proteins, MIWI and MILI, receive proce...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1281
更新日期:2019-03-18 00:00:00
abstract::Melting of DNA in a segment bounded at both ends by regions of greater stability during electrophoresis in denaturing gradient gels show complex properties, not accommodated with standard melting theory. Compact bands of some DNA molecules become anomalously broadened at the retardation level in a denaturing gradient,...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.14.2775
更新日期:1995-07-25 00:00:00
abstract::Hepatitis C virus (HCV) RNA is recognized and cleaved in vitro by RNase P enzyme near the AUG start codon. Because RNase P identifies transfer RNA (tRNA) precursors, it has been proposed that HCV RNA adopts structural similarities to tRNA. Here, we present experimental evidence of RNase P sensitivity conservation in n...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki290
更新日期:2005-03-08 00:00:00