DNA stretching on functionalized gold surfaces.

abstract：:Alternative splicing has recently emerged as a major mechanism of regulation in the human genome, occurring in perhaps 40-60% of human genes. Thus, microarray studies of functional regulation could, in principle, be extended to detect not only the changes in the overall expression of a gene, but also changes in its sp...

journal_title：Nucleic acids research

authors： Le K,Mitsouras K,Roy M,Wang Q,Xu Q,Nelson SF,Lee C

更新日期：2004-12-14 00:00:00

abstract：:Addition of thiol compounds containing an anionic group to the 3'-terminal unsaturated sugar of the 5' fragment obtained from an oligonucleotide containing an AP site cleaved by beta-elimination, can be followed by gel electrophoresis. The technique enables to distinguish between two mechanisms of cleavage of the C3'-...

journal_title：Nucleic acids research

authors： Bailly V,Verly WG

更新日期：1988-10-25 00:00:00

abstract：:Rad18 is a ubiquitin E3 ligase that monoubiquitinates PCNA on stalled replications forks. This allows recruitment of damage-tolerant polymerases for damage bypass and DNA repair. In this activity, the Rad18 protein has to interact with Rad6, the E2 ubiquitin-conjugating enzyme, ubiquitin, PCNA and DNA. Here we analyze...

journal_title：Nucleic acids research

authors： Notenboom V,Hibbert RG,van Rossum-Fikkert SE,Olsen JV,Mann M,Sixma TK

更新日期：2007-01-01 00:00:00

abstract：:A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisel...

journal_title：Nucleic acids research

authors： Jaye M,de la Salle H,Schamber F,Balland A,Kohli V,Findeli A,Tolstoshev P,Lecocq JP

更新日期：1983-04-25 00:00:00

abstract：:Scanning tunneling microscopy (STM) reveals nanometer scale details of hydrated DNA but the interpretation of the images is controversial because of substrate artifacts and the lack of a theory for image contrast. We demonstrate that we have overcome these problems by identifying five DNA samples by their STM images a...

journal_title：Nucleic acids research

authors： Jeffrey AM,Jing TW,DeRose JA,Vaught A,Rekesh D,Lu FX,Lindsay SM

更新日期：1993-12-25 00:00:00

abstract：:Pax-5, a member of the paired domain family of transcription factors, is a key regulator of B lymphocyte-specific transcription and differentiation. A major target of Pax-5-mediated activation is the mb-1 gene, which encodes the essential transmembrane signaling protein Ig-alpha. Pax-5 recruits three members of the Et...

journal_title：Nucleic acids research

authors： Maier H,Ostraat R,Parenti S,Fitzsimmons D,Abraham LJ,Garvie CW,Hagman J

更新日期：2003-10-01 00:00:00

abstract：:Polyploidy, the presence of multiple sets of chromosomes that are similar but not identical, complicates both chromosome walking and assembly of sequence-ready contigs for many plant taxa including a large number of economically-significant crops. Traditional 'dot-blot hybridization' or PCR-based assays for identifyin...

journal_title：Nucleic acids research

authors： Lin YR,Draye X,Qian X,Ren S,Zhu LH,Tomkins J,Wing RA,Li Z,Paterson AH

更新日期：2000-04-01 00:00:00

abstract：:DNA-PKcs and Ku are essential components of the complex that catalyzes non-homologous end joining (NHEJ) of DNA double-strand breaks (DSBs). Ku, a heterodimeric protein, binds to DNA ends and facilitates recruitment of the catalytic subunit, DNA-PKcs. We have investigated the effect of DNA strand orientation and seque...

journal_title：Nucleic acids research

authors： Pawelczak KS,Andrews BJ,Turchi JJ

更新日期：2005-01-07 00:00:00

abstract：:N-(guanosin-8-yl) acetylaminofluorene (Guo-AAF) was prepared by the reaction of N-acetoxy-N-2-acetylaminofluorene (AAAF) and guanosine. Antibodies to Guo-AAF were elicited in rabbits by immunization with bovine serum albumin-Guo-AAF conjugate. The antibodies were purified by affinity chromatography on a Sepharose-Guo-...

journal_title：Nucleic acids research

authors： Guigues M,Leng M

更新日期：1979-02-01 00:00:00

abstract：:For characterizing in vivo functions of a mammalian protein, it is informative to obtain conditional mutations and apply them to the mouse genetic system. However, the isolation of conditional mutations has been quite difficult in cultured cells. We report here that functional expression of a heterologous mammalian ge...

journal_title：Nucleic acids research

authors： Adachi N,Ikeda H,Kikuchi A

更新日期：1994-10-11 00:00:00

abstract：:The secondary structure of V4, the largest variable area of eukaryotic small subunit ribosomal RNA, was re-examined by comparative analysis of 3253 nucleotide sequences distributed over the animal, plant and fungal kingdoms and a diverse set of protist taxa. An extensive search for compensating base pair substitutions...

journal_title：Nucleic acids research

authors： Wuyts J,De Rijk P,Van de Peer Y,Pison G,Rousseeuw P,De Wachter R

更新日期：2000-12-01 00:00:00

abstract：:A problem in EST clustering is the presence of repeat sequences. To avoid false matches, repeats have to be masked. This can be a time-consuming process, and it depends on available repeat libraries. We present a fast and effective method that aims to eliminate the problems repeats cause in the process of clustering. ...

journal_title：Nucleic acids research

authors： Schneeberger K,Malde K,Coward E,Jonassen I

更新日期：2005-04-14 00:00:00

abstract：:The widely accepted model for the processing of tRNAs in Escherichia coli involves essential initial cleavages by RNase E within polycistronic transcripts to generate pre-tRNAs that subsequently become substrates for RNase P. However, recently we identified two polycistronic tRNA transcripts whose endonucleolytic proc...

journal_title：Nucleic acids research

authors： Mohanty BK,Kushner SR

更新日期：2008-02-01 00:00:00

abstract：:Type I CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated) systems exist in bacterial and archaeal organisms and provide immunity against foreign DNA. The Cas protein content of the DNA interference complexes (termed Cascade) varies between different CRISPR-Cas subtypes. A minima...

journal_title：Nucleic acids research

authors： Dwarakanath S,Brenzinger S,Gleditzsch D,Plagens A,Klingl A,Thormann K,Randau L

更新日期：2015-10-15 00:00:00

abstract：:Several lines of evidence indicate that differences in DNA repair capacity are an important source of variability in cancer risk. However, traditional assays for measurement of DNA repair activity in human samples are laborious and time-consuming. DNA glycosylases are the first step in base excision repair of a variet...

journal_title：Nucleic acids research

authors： Collini M,Caccia M,Chirico G,Barone F,Dogliotti E,Mazzei F

更新日期：2005-10-21 00:00:00

abstract：:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. PHF1 [plant homeodomain (PHD) finger protein 1], which contains two kinds of histone reader modules, a Tudor domain and two PHD fin...

journal_title：Nucleic acids research

authors： Liu R,Gao J,Yang Y,Qiu R,Zheng Y,Huang W,Zeng Y,Hou Y,Wang S,Leng S,Feng D,Yu W,Sun G,Shi H,Teng X,Wang Y

更新日期：2018-07-27 00:00:00

abstract：:Cytosines in genomic DNA are sometimes methylated. This affects many biological processes and diseases. The standard way of measuring methylation is to use bisulfite, which converts unmethylated cytosines to thymines, then sequence the DNA and compare it to a reference genome sequence. We describe a method for the cri...

journal_title：Nucleic acids research

authors： Frith MC,Mori R,Asai K

更新日期：2012-07-01 00:00:00

abstract：:The oncogene BMI1 encodes a polycomb group transcription factor that is required for embryonic development and self-renewal of stem cells. Despite these important functions little is known about the regulation of BMI1 expression. A cDNA microarray based search for target genes of E2F-1 in neuroblastoma cells expressin...

journal_title：Nucleic acids research

authors： Nowak K,Kerl K,Fehr D,Kramps C,Gessner C,Killmer K,Samans B,Berwanger B,Christiansen H,Lutz W

更新日期：2006-03-31 00:00:00

abstract：:The behaviour of highly purified bacterial expressed rat O6-methylguanine-DNA methyltransferase (MGMT) towards the repair of CGCm6GAGCTCGCG and CGCe6GAGCTCGCG (km6G/ke6G = 1.45, where k is the second order repair rate constant determined, m6G and e6G are O6-methyl and O6-ethylguanine) is similar to that of E. coli 39k...

journal_title：Nucleic acids research

authors： Liem LK,Lim A,Li BF

更新日期：1994-05-11 00:00:00

abstract：:CENP-A and CENP-B are major components of centromeric chromatin. CENP-A is the histone H3 variant, which forms the centromere-specific nucleosome. CENP-B specifically binds to the CENP-B box DNA sequence on the centromere-specific repetitive DNA. In the present study, we found that the CENP-A nucleosome more stably re...

journal_title：Nucleic acids research

authors： Fujita R,Otake K,Arimura Y,Horikoshi N,Miya Y,Shiga T,Osakabe A,Tachiwana H,Ohzeki J,Larionov V,Masumoto H,Kurumizaka H

更新日期：2015-05-26 00:00:00

abstract：:The University of Minnesota Biocatalysis/Biodegradation Database (http://www.labmed.umn.edu/umbbd/ ) begins its fifth year having met its initial goals. It contains approximately 100 pathways for microbial catabolic metabolism of primarily xenobiotic organic compounds, including information on approximately 650 reacti...

journal_title：Nucleic acids research

authors： Ellis LB,Hershberger CD,Wackett LP

更新日期：2000-01-01 00:00:00

abstract：:Gene duplication is an important process in evolution. The availability of genome sequences of a number of organisms has made it possible to conduct comprehensive searches for duplicated genes enabling informative studies of their evolution. We have established the FGF (Fishing Gene Family) program to efficiently sear...

journal_title：Nucleic acids research

authors： Zheng H,Shi J,Fang X,Li Y,Vang S,Fan W,Wang J,Zhang Z,Wang W,Kristiansen K,Wang J

更新日期：2007-07-01 00:00:00

abstract：:Spectroscopic studies of the complexes of double-stranded (ds) DNA with the polymethylene-amine linked heterodimers thiazole orange-thiazole blue, thiazole orange-ethidium, and fluorescein-ethidium, in each case show efficient energy transfer from donor to acceptor chromophores (Benson, S.C., Singh, P. and Glazer, A.N...

journal_title：Nucleic acids research

authors： Benson SC,Mathies RA,Glazer AN

更新日期：1993-12-11 00:00:00

abstract：:We investigate the role of water molecules in 89 protein-RNA complexes taken from the Protein Data Bank. Those with tRNA and single-stranded RNA are less hydrated than with duplex or ribosomal proteins. Protein-RNA interfaces are hydrated less than protein-DNA interfaces, but more than protein-protein interfaces. Majo...

journal_title：Nucleic acids research

authors： Barik A,Bahadur RP

更新日期：2014-09-01 00:00:00

abstract：:Proper expression of the genes of the human beta-globin gene locus requires the associated locus control region (LCR). Structurally, the LCR is defined by the presence of four domains of erythroid-specific chromatin structure. These domains, which have been characterized as DNase I hypersensitive sites (HSs), comprise...

journal_title：Nucleic acids research

authors： Pomerantz O,Goodwin AJ,Joyce T,Lowrey CH

更新日期：1998-12-15 00:00:00

abstract：:When B cells differentiate into plasma cells, there is a large increase in the cellular content of mRNA coding for immunoglobulin. This increase cannot be fully accounted for by the increase in rate of transcription of the genes. We have investigated the possibility that the half-life of mu heavy chain mRNA increases ...

journal_title：Nucleic acids research

authors： Cox A,Emtage JS

更新日期：1989-12-25 00:00:00

abstract：:A vexing difficulty of the RNA world hypothesis is how RNA molecules of significant complexity could ever have evolved given their susceptibility to degradation. One way degradation might have been reduced is through low temperature. Here we report that truncated and fragmented derivatives of the hairpin ribozyme can ...

journal_title：Nucleic acids research

authors： Vlassov AV,Johnston BH,Landweber LF,Kazakov SA

更新日期：2004-05-25 00:00:00

abstract：:Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand viral disease, detection and identification of these viruses are essential. Although PCR is widely used for rapid virus identification due to its low cost and high sensitivity and speci...

journal_title：Nucleic acids research

authors： Kim H,Kang N,An K,Kim D,Koo J,Kim MS

更新日期：2017-01-04 00:00:00

abstract：:A series of plasmids were constructed in which a promoter was introduced into a lac-based operon fusion vector. A perfectly symmetrical oligonucleotide of 22-bp corresponding to an idealized binding site for cAMP receptor protein (CRP) of E. coli was chemically synthesized. The synthetic CRP site was placed between th...

journal_title：Nucleic acids research

authors： Morita T,Shigesada K,Kimizuka F,Aiba H

更新日期：1988-08-11 00:00:00

abstract：:We have previously shown that hedamycin, a GC-rich DNA-binding antitumor agent, downregulates survivin transcription (Wu et al. (2005) Molecular mechanism of inhibition of survivin transcription by the GC-rich sequence selective DNA-binding antitumor agent, hedamycin: evidence of survivin downregulation associated wit...

journal_title：Nucleic acids research

authors： Wu J,Apontes P,Song L,Liang P,Yang L,Li F

更新日期：2007-01-01 00:00:00