Abstract:
:We have previously reported the human cDNA encoding MSSP-1, a sequence-specific double- and single-stranded DNA binding protein [Negishi, Nishita, Saëgusa, Kakizaki, Galli, Kihara, Tamai, Miyajima, Iguchi-Ariga and Ariga (1994) Oncogene, 9, 1133-1143]. MSSP-1 binds to a DNA replication origin/transcriptional enhancer of the human c-myc gene and has turned out to be identical with Scr2, a human protein which complements the defect of cdc2 kinase in S.pombe [Kataoka and Nojima (1994) Nucleic Acid Res., 22, 2687-2693]. We have cloned the cDNA for MSSP-2, another member of the MSSP family of proteins. The MSSP-2 cDNA shares highly homologous sequences with MSSP-1 cDNA, except for the insertion of 48 bp coding 16 amino acids near the C-terminus. Like MSSP-1, MSSP-2 has RNP-1 consensus sequences. The results of the experiments using bacterially expressed MSSP-2, and its deletion mutants, as histidine fusion proteins suggested that the binding specificity of MSSP-2 to double- and single-stranded DNA is the same as that of MSSP-1, and that the RNP consensus sequences are required for the DNA binding of the protein. MSSP-2 stimulated the DNA replication of an SV40-derived plasmid containing the binding sequence for MSSP-1 or -2. MSSP-2 is hence suggested to play an important role in regulation of DNA replication.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Takai T,Nishita Y,Iguchi-Ariga SM,Ariga Hdoi
10.1093/nar/22.25.5576subject
Has Abstractpub_date
1994-12-25 00:00:00pages
5576-81issue
25eissn
0305-1048issn
1362-4962journal_volume
22pub_type
杂志文章abstract::Many mutations occur as a result of DNA synthesis past the site of DNA damage by DNA damage bypass polymerases. The frequency and types of mutations not only depend on the nature of the damage, but also on the sequence context, as revealed from analysis of mutation spectra of DNA exposed to mutagens. Herein we report ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn595
更新日期:2008-10-01 00:00:00
abstract::The current methods for quantifying genome-wide 5-methylcytosine (5mC) oxides are still scarce, mostly restricted with two limitations: assay sensitivity is seriously compromised with cost, assay time and sample input; epigenetic information is irreproducible during polymerase chain reaction (PCR) amplification withou...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz704
更新日期:2019-11-04 00:00:00
abstract::DNA methylation is the best-studied epigenetic modification and describes the conversion of cytosine to 5-methylcytosine. The importance of this phenomenon is that aberrant promoter hypermethylation is a common occurrence in cancer and is frequently associated with gene silencing. Various techniques are currently avai...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnj017
更新日期:2006-02-07 00:00:00
abstract::Investigation of biological processes using selective chemical interventions is generally applied in biomedical research and drug discovery. Many studies of this kind make use of gene expression experiments to explore cellular responses to chemical interventions. Recently, some research groups constructed libraries of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn610
更新日期:2008-11-01 00:00:00
abstract::Polyadenylation is a co-transcriptional process that modifies mRNA 3'-ends in eukaryotes. In yeast, CF IA and CPF constitute the core 3'-end maturation complex. CF IA comprises Rna14p, Rna15p, Pcf11p and Clp1p. CF IA interacts with the C-terminal domain of RNA Pol II largest subunit via Pcf11p which links pre-mRNA 3'-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr800
更新日期:2012-02-01 00:00:00
abstract::Clustered regularly interspaced short palindromic repeats (CRISPRs) and their associated Cas proteins comprise a prokaryotic RNA-guided adaptive immune system that interferes with mobile genetic elements, such as plasmids and phages. The type I-E CRISPR interference complex Cascade from Escherichia coli is composed of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1285
更新日期:2015-01-01 00:00:00
abstract::The InParanoid eukaryotic ortholog database (http://InParanoid.sbc.su.se/) has been updated to version 6 and is now based on 35 species. We collected all available 'complete' eukaryotic proteomes and Escherichia coli, and calculated ortholog groups for all 595 species pairs using the InParanoid program. This resulted ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1020
更新日期:2008-01-01 00:00:00
abstract::The Human Genome Project has facilitated the sequencing of many species, yet the current Sanger method is too expensive, labor intensive and time consuming to accomplish medical resequencing of human genomes en masse. Of the 'next-generation' technologies, cyclic reversible termination (CRT) is a promising method with...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm689
更新日期:2007-01-01 00:00:00
abstract::Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand viral disease, detection and identification of these viruses are essential. Although PCR is widely used for rapid virus identification due to its low cost and high sensitivity and speci...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1095
更新日期:2017-01-04 00:00:00
abstract::The Ensembl project (https://www.ensembl.org) annotates genomes and disseminates genomic data for vertebrate species. We create detailed and comprehensive annotation of gene structures, regulatory elements and variants, and enable comparative genomics by inferring the evolutionary history of genes and genomes. Our int...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa942
更新日期:2021-01-08 00:00:00
abstract::Small RNA (sRNA) Sequencing technology has revealed that microRNAs (miRNAs) are capable of exhibiting frequent variations from their canonical sequences, generating multiple variants: the isoforms of miRNAs (isomiRs). However, integrated tool to precisely detect and systematically annotate isomiRs from sRNA sequencing...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw427
更新日期:2016-07-08 00:00:00
abstract::Genome-wide mapping in the identification of novel candidate genes has always been the standard method in genetics and genomics to correlate a clinically interesting phenotypic trait with a genotype. However, the performance of a mapping experiment using classical microsatellite approaches can be very time consuming. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki430
更新日期:2005-07-01 00:00:00
abstract::Revealing the clonal composition of a single tumor is essential for identifying cell subpopulations with metastatic potential in primary tumors or with resistance to therapies in metastatic tumors. Sequencing technologies provide only an overview of the aggregate of numerous cells. Computational approaches to de-mix a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt641
更新日期:2013-09-01 00:00:00
abstract::The DNA-binding specificity and affinity of the dimeric human transcription factor (TF) STAT1, were assessed by total internal reflectance fluorescence protein-binding microarrays (TIRF-PBM) to evaluate the effects of protein phosphorylation, higher-order polymerization and small-molecule inhibition. Active, phosphory...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1085
更新日期:2013-01-01 00:00:00
abstract::Bacillus subtilis plasmid pGR71 is a promoter-probe shuttle vector derived from pUB110. The expression of the cat gene on pGR71 in B. subtilis requires the insertion of a Bacillus promoter and a ribosomal binding site (RBS) into the HindIII cloning site immediately upstream from the cat gene. A recombinant plasmid of ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.4.739
更新日期:1990-02-25 00:00:00
abstract::The RESID Database is a comprehensive collection of annotations and structures for protein post-translational modifications including N-terminal, C-terminal and peptide chain cross-link modifications. The RESID Database includes systematic and frequently observed alternate names, Chemical Abstracts Service registry nu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.1.199
更新日期:2001-01-01 00:00:00
abstract::Acinetobacter sp. strain ADP1 is a nutritionally versatile soil bacterium closely related to representatives of the well-characterized Pseudomonas aeruginosa and Pseudomonas putida. Unlike these bacteria, the Acinetobacter ADP1 is highly competent for natural transformation which affords extraordinary convenience for ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh910
更新日期:2004-10-28 00:00:00
abstract::mRNA expression involves transcription initiation, elongation and degradation. In cells, these dynamic processes are highly regulated. However, experimental characterization of the dynamic processes in vivo is difficult due to the paucity of methods capable of direct measurements. We present a highly sensitive and ver...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw596
更新日期:2016-10-14 00:00:00
abstract::Genes from yeast to mammals are frequently subject to non-coding transcription of their antisense strand; however the genome-wide role for antisense transcription remains elusive. As transcription influences chromatin structure, we took a genome-wide approach to assess which chromatin features are associated with nasc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv666
更新日期:2015-09-18 00:00:00
abstract::REBASE is a comprehensive database of information about restriction enzymes and their associated methylases, including their recognition and cleavage sites and their commercial availability. Also included is a listing of homing endonucleases. Information from REBASE is available via monthly electronic mailings as well...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.1.338
更新日期:1998-01-01 00:00:00
abstract::G-quadruplexes play various roles in multiple biological processes, which can be positive when a G4 is involved in the regulation of gene expression or detrimental when the folding of a stable G4 impairs DNA replication promoting genome instability. This duality interrogates the significance of their presence within g...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz463
更新日期:2019-07-09 00:00:00
abstract::Cancer-associated somatic mutations outside protein-coding regions remain largely unexplored. Analyses of the TERT locus have indicated that non-coding regulatory mutations can be more frequent than previously suspected and play important roles in oncogenesis. Using a computational method called SASE-hunter, developed...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv419
更新日期:2015-06-23 00:00:00
abstract::Telomerase is a ribonucleoprotein reverse transcriptase that synthesizes and maintains telomeric DNA. Studies of telomeres and telomerase are facilitated by the large number of linear DNA molecules found in ciliated protozoa, such as Tetrahymena thermophila. To examine the expression of telomerase, we investigated the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.21.4269
更新日期:1999-11-01 00:00:00
abstract::We report that greA expression is driven by two strong, overlapping P1 and P2 promoters. The P1 promoter is sigma(70)-dependent and P2 is sigma(E)-dependent. Two-thirds of transcripts terminate within the leader region and the remaining third comprises greA mRNA. Termination efficiency seems to be unaffected by growth...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1150
更新日期:2010-03-01 00:00:00
abstract::In highly oncogenic adenovirus (Ad) 12-transformed cells, major histocompatibility complex (MHC) class I gene expression is down-regulated by the products of the viral E1A oncogene at the level of initiation of transcription. However, class I gene expression is unaltered or elevated in non-oncogenic Ad2- or Ad5-transf...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.22.4779
更新日期:1994-11-11 00:00:00
abstract::Two types of different repetitive DNA sequences called RepMP1 and RepMP2 were identified in the genome of Mycoplasma pneumoniae. The number of these repeated elements, their nucleotide sequence and their localization on a physical map of the M. pneumoniae genome were determined. The results show that RepMP1 appears at...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.17.8337
更新日期:1988-09-12 00:00:00
abstract::We have sequenced the Escherichia coli grpE gene and shown that it encodes a 197-amino acid residue protein of 21,668-Mr. The predicted N-terminal amino acid sequence, as well as the overall amino acid composition agree well with that of the purified protein. From Northern analysis, we have shown that transcription of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.15.7545
更新日期:1988-08-11 00:00:00
abstract::We have established a sensitive, monoclonal antibody (Mab)-based procedure permitting the selective enrichment of sequences containing the miscoding alkylation product O6-ethylguanine (O6-EtGua) from mammalian DNA. H5 rat hepatoma cells were reacted with the N-nitroso carcinogen N-ethyl-N-nitrosourea in vitro, to give...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.16.4467
更新日期:1991-08-25 00:00:00
abstract::Recognizing the methylation status of specific DNA sequences is central to the function of many systems in eukaryotes and prokaryotes. Restriction-modification systems have to distinguish between 'self' and 'non-self' DNA and depend on the inability of restriction endonucleases to cleave their DNA substrates when the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.16.3143
更新日期:2000-08-15 00:00:00
abstract::Motif3D is a web-based protein structure viewer designed to allow sequence motifs, and in particular those contained in the fingerprints of the PRINTS database, to be visualised on three-dimensional (3D) structures. Additional functionality is provided for the rhodopsin-like G protein-coupled receptors, enabling finge...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg534
更新日期:2003-07-01 00:00:00