Monoclonal antibody-based, selective isolation of DNA fragments containing an alkylated base to be quantified in defined gene sequences.

abstract：:The Functional Single Nucleotide Polymorphism (F-SNP) database integrates information obtained from 16 bioinformatics tools and databases about the functional effects of SNPs. These effects are predicted and indicated at the splicing, transcriptional, translational and post-translational level. As such, the database h...

journal_title：Nucleic acids research

authors： Lee PH,Shatkay H

更新日期：2008-01-01 00:00:00

abstract：:SITEHOUND-web (http://sitehound.sanchezlab.org) is a binding-site identification server powered by the SITEHOUND program. Given a protein structure in PDB format SITEHOUND-web will identify regions of the protein characterized by favorable interactions with a probe molecule. These regions correspond to putative ligand...

journal_title：Nucleic acids research

authors： Hernandez M,Ghersi D,Sanchez R

更新日期：2009-07-01 00:00:00

abstract：:Residual dipolar coupling (RDC) represents one of the most exciting emerging NMR techniques for protein structure studies. However, solving a protein structure using RDC data alone is still a highly challenging problem. We report here a computer program, RDC-PROSPECT, for protein structure prediction based on a struct...

journal_title：Nucleic acids research

authors： Qu Y,Guo JT,Olman V,Xu Y

更新日期：2004-01-26 00:00:00

abstract：:Exon arrays are regularly used to analyze differential splicing events. GeneChip Gene 1.0 ST Arrays (gene arrays) manufactured by Affymetrix, Inc. are primarily used to determine expression levels of transcripts, although their basic design is rather similar to GeneChip Exon 1.0 ST Arrays (exon arrays). Here, we show ...

journal_title：Nucleic acids research

authors： Gellert P,Teranishi M,Jenniches K,De Gaspari P,John D,Kreymborg Kg,Braun T,Uchida S

更新日期：2012-03-01 00:00:00

abstract：:Alternative splicing constitutes a major mechanism creating protein diversity in humans. This diversity can result from the alternative skipping of entire exons or by alternative selection of the 5' or 3' splice sites that define the exon boundaries. In this study, we analyze the sequence and evolutionary characterist...

journal_title：Nucleic acids research

authors： Akerman M,Mandel-Gutfreund Y

更新日期：2007-01-01 00:00:00

abstract：:Prostatic steroid binding protein, whose expression is stimulated by androgens, consists of two subunits: one containing the polypeptides C1 and C3 and the other containing C2 and C3. We have characterised genomic clones containing the C1 and C2 genes by restriction enzyme analysis and DNA sequencing. Both genes are 3...

journal_title：Nucleic acids research

authors： Parker M,Needham M,White R,Hurst H,Page M

更新日期：1982-09-11 00:00:00

abstract：:Microarrays are an effective tool for monitoring genome-wide gene expression levels. In current microarray analyses, the majority of genes on arrays are frequently eliminated for further analysis because the changes in their expression levels (ratios) are considered to be not significant. This strategy risks failure t...

journal_title：Nucleic acids research

authors： Yano K,Imai K,Shimizu A,Hanashita T

更新日期：2006-03-14 00:00:00

abstract：:The nucleotide sequence of 5.8S rRNA from the Chinese silkworm Philosamia cynthia ricini has been determined by gel sequencing and mobility shift methods. The complete primary structure is (sequence in text). This is one of the largest known 5.8S rRNAs. As compared to Bombyx 5.8S rRNA, it is two nucleotides longer; t...

journal_title：Nucleic acids research

authors： Feng YX,Krupp G,Gross HJ

更新日期：1982-10-25 00:00:00

abstract：:The VS ribozyme trans-cleavage substrate interacts with the catalytic RNA via tertiary interactions. To study the role of phosphate groups in the ribozyme-substrate interaction, 18 modified substrates were synthesized, where an epimeric phosphorothioate replaces one of the phosphate diester linkages. Sites in the stem...

journal_title：Nucleic acids research

authors： Kovacheva YS,Tzokov SB,Murray IA,Grasby JA

更新日期：2004-12-01 00:00:00

abstract：:The synthesis of cyclic di-, tri-, tetra-, penta- and hexa-thymidylic acids (16; n = 2, 3, 4, 5 and 6) and the cyclic hexadeoxyribonucleotide [d(CpCpTpApGpGp)], via the "filtration" approach, is reported. Some of the physical properties of the cyclic oligonucleotides are discussed, and their susceptibility to digestio...

journal_title：Nucleic acids research

authors： Rao MV,Reese CB

更新日期：1989-10-25 00:00:00

abstract：:High-throughput chromosome conformation capture (3C) technologies, such as Hi-C, have made it possible to survey 3D genome structure. However, obtaining 3D profiles at kilobase resolution at low cost remains a major challenge. Therefore, we herein present an algorithm for precise identification of chromatin interactio...

journal_title：Nucleic acids research

authors： Zhang H,Li F,Jia Y,Xu B,Zhang Y,Li X,Zhang Z

更新日期：2017-12-15 00:00:00

abstract：:Expression of the rpoBC genes encoding the beta and beta' RNA polymerase subunits of Escherichia coli is autogenously regulated. Although previous studies have demonstrated a post-transcriptional feedback mechanism, complex transcriptional controls of rpoBC expression may also contribute. We show that an attenuator (r...

journal_title：Nucleic acids research

authors： Steward KL,Linn T

更新日期：1992-09-25 00:00:00

abstract：:The p-n-butylphenyl- and p-n-butylanilino- substituted analogs of dGTP and dATP, respectively, were tested as inhibitors of purified human placental DNA polymerases alpha and delta. It was observed that DNA polymerase alpha activity was potently inhibited by these analogs with I0.5 values as low as the nanomolar range...

journal_title：Nucleic acids research

authors： Lee MY,Toomey NL,Wright GE

更新日期：1985-12-09 00:00:00

abstract：:U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1gamma (hF1gamma) intron 9 in vitro. Moreover, h...

journal_title：Nucleic acids research

authors： Fukumura K,Taniguchi I,Sakamoto H,Ohno M,Inoue K

更新日期：2009-04-01 00:00:00

abstract：:Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoure...

journal_title：Nucleic acids research

authors： Yeoh LM,Goodman CD,Hall NE,van Dooren GG,McFadden GI,Ralph SA

更新日期：2015-05-19 00:00:00

abstract：:Functionality of the non-coding transcripts encoded by the human genome is the coveted goal of the modern genomics research. While commonly relied on the classical methods of forward genetics, integration of different genomics datasets in a global Systems Biology fashion presents a more productive avenue of achieving ...

journal_title：Nucleic acids research

authors： St Laurent G,Vyatkin Y,Antonets D,Ri M,Qi Y,Saik O,Shtokalo D,de Hoon MJ,Kawaji H,Itoh M,Lassmann T,Arner E,Forrest AR,FANTOM consortium.,Nicolas E,McCaffrey TA,Carninci P,Hayashizaki Y,Wahlestedt C,Kapranov P

更新日期：2016-04-20 00:00:00

abstract：:Phenotype analysis is commonly recognized to be of great importance for gaining insight into genetic interaction underlying inherited diseases. However, few computational contributions have been proposed for this purpose, mainly owing to lack of controlled clinical information easily accessible and structured for comp...

journal_title：Nucleic acids research

authors： Masseroli M,Galati O,Pinciroli F

更新日期：2005-07-01 00:00:00

abstract：:The miR-290 cluster is expressed in embryonic stem cells (ESCs) and is important for the maintenance of pluripotency, but little is known about the mechanisms regulating the early embryonic microRNA cluster (EEmiRC) expression. Here we report the identification of a 332-bp intragenic enhancer (IE) able to modulate the...

journal_title：Nucleic acids research

authors： Tata PR,Tata NR,Kühl M,Sirbu IO

更新日期：2011-05-01 00:00:00

abstract：:A Fortran computer algorithm has been used to analyze the nucleotide sequence of several structural genes. The analysis performed on both coding and complementary DNA strands shows that whereas open reading frames shorter than 100 codons are randomly distributed on both DNA strands, open reading frames longer than 100...

journal_title：Nucleic acids research

authors： Casino A,Cipollaro M,Guerrini AM,Mastrocinque G,Spena A,Scarlato V

更新日期：1981-03-25 00:00:00

abstract：:Although Dicer is essential for general microRNA (miRNA) biogenesis, vertebrate mir-451 is Dicer independent. Instead, its short pre-miRNA hairpin is 'sliced' by Ago2, then 3'-resected into mature miRNAs. Here, we show that Drosophila cells and animals generate functional small RNAs from mir-451-type precursors. Howev...

journal_title：Nucleic acids research

authors： Yang JS,Smibert P,Westholm JO,Jee D,Maurin T,Lai EC

更新日期：2014-02-01 00:00:00

abstract：:To prevent progressive telomere shortening as a result of conventional DNA replication, new telomeric DNA must be added onto the chromosome end. The de novo DNA synthesis involves elongation of the G-rich strand of the telomere by telomerase. In human cells, the CST complex (CTC1-STN1-TEN1) also functions in telomere ...

journal_title：Nucleic acids research

authors： Feng X,Hsu SJ,Kasbek C,Chaiken M,Price CM

更新日期：2017-05-05 00:00:00

abstract：:Initial steps in the synthesis of functional tRNAs require 5'- and 3'-processing of precursor tRNAs (pre-tRNAs), which in yeast mitochondria are achieved by two endonucleases, RNase P and RNase Z. In this study, using a combination of detergent-free Blue Native Gel Electrophoresis, proteomics and in vitro testing of p...

journal_title：Nucleic acids research

authors： Daoud R,Forget L,Lang BF

更新日期：2012-02-01 00:00:00

abstract：:A modification of previously described methods of electron microscopic gene mapping and of gene enrichment based on the avidin-biotin interaction is presented. The modification consists of coupling cytochrome-c instead of pentane diamine to the oxidized 2', 3' terminus of an RNA by Schiff base formation and BH-4 reduc...

journal_title：Nucleic acids research

authors： Sodja A,Davidson N

更新日期：1978-02-01 00:00:00

abstract：:The distribution and amount of 5-methylcytosine (5-MeCyt) in DNA was measured for early embryos of mouse strain CF1 (2 to 4 cell stage to blastocyst) and mouse teratocarcinoma cells. In each case, the pattern of methylation was examined by use of the restriction enzymes Hha I and HPA II HPA II, which cut DNA at the si...

journal_title：Nucleic acids research

authors： Singer J,Roberts-Ems J,Luthardt FW,Riggs AD

更新日期：1979-12-20 00:00:00

abstract：:While antibiotic selection has been routinely used for the selection of genetically modified cells, administration of cytotoxic drugs often leads to deleterious effects not only to inert cells but also to transfected or transduced ones. In this study, we propose an Antigen-MEdiated Genetically modified cell Amplificat...

journal_title：Nucleic acids research

authors： Kawahara M,Ueda H,Morita S,Tsumoto K,Kumagai I,Nagamune T

更新日期：2003-04-01 00:00:00

abstract：:The restriction endonuclease (R.BsuFI) of Bacillus subtilis recognizes the target DNA sequence 5' CCGG. The R.BsuFI gene was found in close proximity to the cognate M.BsuFI gene, which had previously been characterized (1). Cloning of the R.BsuFI gene in E.coli was only possible with the M.BsuFI Mtase gene present on ...

journal_title：Nucleic acids research

authors： Kapfer W,Walter J,Trautner TA

更新日期：1991-12-11 00:00:00

abstract：:We have developed general methods for joining together, via cleavable disulfide bonds, either two unprotected polynucleotides or a polynucleotide and a peptide or protein. To join two oligonucleotides, each is first converted to an adduct in which cystamine is joined to the 5'-terminal phosphate of the oligonucleotide...

journal_title：Nucleic acids research

authors： Chu BC,Orgel LE

更新日期：1988-05-11 00:00:00

abstract：:A method is described to simultaneously amplify and sequence DNA using a new class of nucleotides containing boron. During the polymerase chain reaction, boron-modified nucleotides, i.e. 2'-deoxynucleoside 5'-alpha-[P-borano]-triphosphates, are incorporated into the product DNA. The boranophosphate linkages are resist...

journal_title：Nucleic acids research

authors： Porter KW,Briley JD,Shaw BR

更新日期：1997-04-15 00:00:00

abstract：:We present a theory of pluralistic and stochastic gene regulation. To bridge the gap between empirical studies and mathematical models, we integrate pre-existing observations with our meta-analyses of the ENCODE ChIP-Seq experiments. Earlier evidence includes fluctuations in levels, location, activity, and binding of ...

journal_title：Nucleic acids research

authors： Salas EN,Shu J,Cserhati MF,Weeks DP,Ladunga I

更新日期：2016-06-02 00:00:00

abstract：:Here we describe a two-stepped photobiotin-based procedure to enrich a target (canine retinal) cDNA library for tissue specific clones without removing those containing repetitive ( SINE ) elements, despite the presence of these elements in the driver population. In a first hybridization excess SINE elements were hybr...

journal_title：Nucleic acids research

authors： Lin CT,Sargan DR

更新日期：1997-11-01 00:00:00