Abstract:
:We have sequenced the Escherichia coli grpE gene and shown that it encodes a 197-amino acid residue protein of 21,668-Mr. The predicted N-terminal amino acid sequence, as well as the overall amino acid composition agree well with that of the purified protein. From Northern analysis, we have shown that transcription of the grpE gene is under heat shock regulation, i.e., there is a rapid and transient increase in the rate of synthesis of grpE mRNA upon a shift-up in temperature. Forty-six bases upstream of the structural gene is a sequence closely related to the consensus heat shock promoter identified by Cowing et al. [Proc. Natl. Acad. Sci. U.S.A, 82, 2679-2683]. We have shown by S1 mapping and RNA sequencing that this is indeed the promoter for the grpE mRNA. It appears that all discernable transcription initiates only from this promoter, even under non-heat shock conditions.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Lipinska B,King J,Ang D,Georgopoulos Cdoi
10.1093/nar/16.15.7545subject
Has Abstractpub_date
1988-08-11 00:00:00pages
7545-62issue
15eissn
0305-1048issn
1362-4962journal_volume
16pub_type
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