Site-specific chromosomal integration of large synthetic constructs.

abstract：:ExPASy (http://www.expasy.org) has worldwide reputation as one of the main bioinformatics resources for proteomics. It has now evolved, becoming an extensible and integrative portal accessing many scientific resources, databases and software tools in different areas of life sciences. Scientists can henceforth access s...

journal_title：Nucleic acids research

authors： Artimo P,Jonnalagedda M,Arnold K,Baratin D,Csardi G,de Castro E,Duvaud S,Flegel V,Fortier A,Gasteiger E,Grosdidier A,Hernandez C,Ioannidis V,Kuznetsov D,Liechti R,Moretti S,Mostaguir K,Redaschi N,Rossier G,Xenarios

更新日期：2012-07-01 00:00:00

abstract：:M13 RF IV DNA may be prepared in vitro to contain phosphorothioate-modified internucleotidic linkages in the (-)strand only. Certain restriction enzymes react with this modified DNA to hydrolyze the (+)strand exclusively when a phosphorothioate linkage occurs at the normal cleavage point in the (-)strand. The reaction...

journal_title：Nucleic acids research

authors： Taylor JW,Ott J,Eckstein F

更新日期：1985-12-20 00:00:00

abstract：:Gut-enriched Krüppel-like factor (GKLF or KLF4) is a pleiotropic (activating and repressive) transcription factor. This study characterizes the mechanisms of transactivation by GKLF. Using a GAL4 fusion assay, the activating domain of murine GKLF was localized to the 109 amino acid residues in the N-terminus. Site-dir...

journal_title：Nucleic acids research

authors： Geiman DE,Ton-That H,Johnson JM,Yang VW

更新日期：2000-03-01 00:00:00

abstract：:DNA predominantly contains Watson-Crick (WC) base pairs, but a non-negligible fraction of base pairs are in the Hoogsteen (HG) hydrogen bonding motif at any time. In HG, the purine is rotated ∼180° relative to the WC motif. The transitions between WC and HG may play a role in recognition and replication, but are diffi...

journal_title：Nucleic acids research

authors： Vreede J,Pérez de Alba Ortíz A,Bolhuis PG,Swenson DWH

更新日期：2019-12-02 00:00:00

abstract：:Genomic DNA of African trypanosomes contains a hypermodified thymidine residue termed base J (beta-d-glucosyl-HOMedU). This modified base is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. The base is synthesized in a two-step pathway. Initially,...

journal_title：Nucleic acids research

authors： Cliffe LJ,Kieft R,Southern T,Birkeland SR,Marshall M,Sweeney K,Sabatini R

更新日期：2009-04-01 00:00:00

abstract：:R spider is a web-based tool for the analysis of a gene list using the systematic knowledge of core pathways and reactions in human biology accumulated in the Reactome and KEGG databases. R spider implements a network-based statistical framework, which provides a global understanding of gene relations in the supplied ...

journal_title：Nucleic acids research

authors： Antonov AV,Schmidt EE,Dietmann S,Krestyaninova M,Hermjakob H

更新日期：2010-07-01 00:00:00

abstract：:To confirm the activity of an initial small molecule 'hit compound' from an activity screening, one needs to probe the structure-activity relationships by testing close analogs. The multi-fingerprint browser presented here (http://dcb-reymond23.unibe.ch:8080/MCSS/) enables one to rapidly identify such close analogs am...

journal_title：Nucleic acids research

authors： Awale M,Reymond JL

更新日期：2014-07-01 00:00:00

abstract：:A fundamental problem with applying Consensus, Weight-Matrix or hidden Markov models as search tools for biosequences is that there is no way to know, from the model, if the modeled sequences display any dependencies between positional identities. In some instances, these dependencies are crucial in correctly acceptin...

journal_title：Nucleic acids research

authors： Ray WC

更新日期：2005-07-01 00:00:00

abstract：:Stabilization of stalled replication forks prevents excessive fork reversal or degradation, which can undermine genome integrity. The WRN protein is unique among the other human RecQ family members to possess exonuclease activity. However, the biological role of the WRN exonuclease is poorly defined. Recently, the WRN...

journal_title：Nucleic acids research

authors： Aiello FA,Palma A,Malacaria E,Zheng L,Campbell JL,Shen B,Franchitto A,Pichierri P

更新日期：2019-07-26 00:00:00

abstract：:The S-adenosylmethionine (AdoMet) analog sinefungin is a natural product antibiotic that inhibits nucleic acid methyltransferases and arrests the growth of unicellular eukarya and eukaryal viruses. The basis for the particular sensitivity of fungi and protozoa to sinefungin is not known. Here we report the isolation a...

journal_title：Nucleic acids research

authors： Zheng S,Shuman S,Schwer B

更新日期：2007-01-01 00:00:00

abstract：:The hybridization of complementary strands of DNA is the underlying principle of all microarray-based techniques for the analysis of DNA variation. In this paper, we study how probe immobilization at surfaces, specifically probe density, influences the kinetics of target capture using surface plasmon resonance (SPR) s...

journal_title：Nucleic acids research

authors： Peterson AW,Heaton RJ,Georgiadis RM

更新日期：2001-12-15 00:00:00

abstract：:Single-stranded DNA-binding proteins (SSBs) support DNA replication by protecting single-stranded DNA from nucleolytic attack, preventing intra-strand pairing events and playing many other regulatory roles within the replisome. Recent developments in single-molecule approaches have led to a revised picture of the repl...

journal_title：Nucleic acids research

authors： Spenkelink LM,Lewis JS,Jergic S,Xu ZQ,Robinson A,Dixon NE,van Oijen AM

更新日期：2019-05-07 00:00:00

abstract：:The single nucleotide polymorphisms (SNPs) in conserved protein regions have been thought to be strong candidates that alter protein functions. Thus, we have developed SNP@Domain, a web resource, to identify SNPs within human protein domains. We annotated SNPs from dbSNP with protein structure-based as well as sequenc...

journal_title：Nucleic acids research

authors： Han A,Kang HJ,Cho Y,Lee S,Kim YJ,Gong S

更新日期：2006-07-01 00:00:00

abstract：:In plants, small interfering RNAs (siRNAs) can trigger a silencing signal that may spread within a tissue to adjacent cells or even systemically to other organs. Movement of the signal is initially limited to a few cells, but in some cases the signal can be amplified and travel over larger distances. How far silencing...

journal_title：Nucleic acids research

authors： de Felippes FF,Ott F,Weigel D

更新日期：2011-04-01 00:00:00

abstract：:Differential display of mRNA is a simple, sensitive and powerful method to identify differentially expressed gene fragments. The main drawback of differential display is the lack of reproducibility and the inability to read and compare complex gels. This issue results from employing unoptimized primer combinations and...

journal_title：Nucleic acids research

authors： Malhotra K,Foltz L,Mahoney WC,Schueler PA

更新日期：1998-02-01 00:00:00

abstract：:A hybrid plasmid was constructed containing beta-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cl...

journal_title：Nucleic acids research

authors： Keshet E,Rosner A,Bernstein Y,Gorecki M,Aviv H

更新日期：1981-01-10 00:00:00

abstract：:At the DNA/RNA level, biological signals are defined by a combination of spatial structures and sequence motifs. Until now, few attempts had been made in writing general purpose search programs that take into account both sequence and structure criteria. Indeed, the most successful structure scanning programs are usua...

journal_title：Nucleic acids research

authors： Billoud B,Kontic M,Viari A

更新日期：1996-04-15 00:00:00

abstract：:A novel resource centre for TP53 mutations and mutants has been developed (http://p53.fr). TP53 gene dysfunction can be found in the majority of human cancer types. The potential use of TP53 mutation as a biomarker for clinical studies or exposome analysis has led to the publication of thousands of reports describing ...

journal_title：Nucleic acids research

authors： Leroy B,Fournier JL,Ishioka C,Monti P,Inga A,Fronza G,Soussi T

更新日期：2013-01-01 00:00:00

abstract：:The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) offers a rich platform for data sharing, publishing and archiving and a globally comprehensive data set for onward use by the scientific community. With a broad scope spanning raw sequencing reads, genome assemblies and functional annotation, the resource...

journal_title：Nucleic acids research

authors： Toribio AL,Alako B,Amid C,Cerdeño-Tarrága A,Clarke L,Cleland I,Fairley S,Gibson R,Goodgame N,Ten Hoopen P,Jayathilaka S,Kay S,Leinonen R,Liu X,Martínez-Villacorta J,Pakseresht N,Rajan J,Reddy K,Rosello M,Silvester N

更新日期：2017-01-04 00:00:00

abstract：:WormBase (https://wormbase.org/) is a mature Model Organism Information Resource supporting researchers using the nematode Caenorhabditis elegans as a model system for studies across a broad range of basic biological processes. Toward this mission, WormBase efforts are arranged in three primary facets: curation, user ...

journal_title：Nucleic acids research

authors： Harris TW,Arnaboldi V,Cain S,Chan J,Chen WJ,Cho J,Davis P,Gao S,Grove CA,Kishore R,Lee RYN,Muller HM,Nakamura C,Nuin P,Paulini M,Raciti D,Rodgers FH,Russell M,Schindelman G,Auken KV,Wang Q,Williams G,Wright AJ

更新日期：2020-01-08 00:00:00

abstract：:Accurate T-cell epitope prediction is a principal objective of computational vaccinology. As a service to the immunology and vaccinology communities at large, we have implemented, as a server on the World Wide Web, a partial least squares-based multivariate statistical approach to the quantitative prediction of peptid...

journal_title：Nucleic acids research

authors： Guan P,Doytchinova IA,Zygouri C,Flower DR

更新日期：2003-07-01 00:00:00

abstract：:In Arabidopsis thaliana, endogenous trans-acting and exogenous siRNA pathways are initiated by the interaction of DRB4 with trigger dsRNA. Further, DCL4:DRB4 complex cleaves the dsRNA into 21 bp siRNA. Understanding molecular determinants and mechanistic details of dsRNA recognition by DRB4 is vital for inducing long-...

journal_title：Nucleic acids research

authors： Chiliveri SC,Aute R,Rai U,Deshmukh MV

更新日期：2017-08-21 00:00:00

abstract：:To prevent progressive telomere shortening as a result of conventional DNA replication, new telomeric DNA must be added onto the chromosome end. The de novo DNA synthesis involves elongation of the G-rich strand of the telomere by telomerase. In human cells, the CST complex (CTC1-STN1-TEN1) also functions in telomere ...

journal_title：Nucleic acids research

authors： Feng X,Hsu SJ,Kasbek C,Chaiken M,Price CM

更新日期：2017-05-05 00:00:00

abstract：:Several fungal species, representatives of all broad groups like basidiomycetes, ascomycetes and phycomycetes, were examined for the nature of repeated DNA sequences by DNA:DNA reassociation studies using hydroxyapatite chromatography. All of the fungal species tested contained 10-20% repeated DNA sequences. There are...

journal_title：Nucleic acids research

authors： Dutta SK

更新日期：1974-11-01 00:00:00

abstract：:A very mild and efficient procedure has been developed for the preparation of C-5 substituted deoxyuridines. The substituent carries a masked primary aliphatic amino group. These compounds are readily converted into their phosphoramidites and can be used to prepare oligonucleotides carrying one or more aliphatic amino...

journal_title：Nucleic acids research

authors： Haralambidis J,Chai M,Tregear GW

更新日期：1987-06-25 00:00:00

abstract：:Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imin...

journal_title：Nucleic acids research

authors： Kierzek R,He L,Turner DH

更新日期：1992-04-11 00:00:00

abstract：:A cDNA that encodes a transcription factor able to recognize the thyroglobulin gene promoter in vitro was isolated from a dog thyroid cDNA expression library in lambda gt11. The library was screened with a multimerized 20 bp-oligonucleotide probe corresponding to the -126 to -107 bp region of the bovine thyroglobulin ...

journal_title：Nucleic acids research

authors： Javaux F,Donda A,Vassart G,Christophe D

更新日期：1991-03-11 00:00:00

abstract：:Large differences in plant genome sizes are mainly due to numerous events of insertions or deletions (indels). The balance between these events determines the evolutionary direction of genome changes. To address the question of what phenomena trigger these alterations, we compared the genomic sequences of two Arabidop...

journal_title：Nucleic acids research

authors： Ziolkowski PA,Koczyk G,Galganski L,Sadowski J

更新日期：2009-06-01 00:00:00

abstract：:From a sarkosyl-insoluble outer membrane fraction prepared from the Helicobacter pylori strain ATCC 43504, 19 proteins could be sequenced N-terminally by Edman degradation. Oligonucleotides were deduced and used for screening of a genomic library. From the isolated genes, five code for different members of a H.pylori ...

journal_title：Nucleic acids research

authors： Peck B,Ortkamp M,Diehl KD,Hundt E,Knapp B

更新日期：1999-08-15 00:00:00

abstract：:Mild ultraviolet irradiation of E. coli ribosomal subunits leads to the formation of a number of intra-RNA cross-links, in addition to the RNA-protein cross-links already reported (see refs. 9, 10). After partial ribonuclease digestion of the RNA from irradiated subunits, complexes containing these intra-RNA cross-lin...

journal_title：Nucleic acids research

authors： Zwieb C,Brimacombe R

更新日期：1980-06-11 00:00:00